Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,340,559 C→A T95T (ACC→ACA yrbG → putative calcium/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,340,5590CA99.2% 520.5 / ‑4.2 134T95T (ACC→ACAyrbGputative calcium/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (68/65);  total (69/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 134 total aligned reads displayed.

GTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTT  >  NC_000913/3340411‑3340706
                                                                                                                                                    |                                                                                                                                                   
gTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGt                                                                                                                                                   <  4:811152/151‑1 (MQ=255)
  aTTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTcc                                                                                                                                                 <  3:24687/151‑1 (MQ=255)
   ttGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTcc                                                                                                                                                 >  2:265477/1‑150 (MQ=255)
     ggTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCAt                                                                                                                                               <  4:1335968/150‑1 (MQ=255)
        aCATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCt                                                                                                                                            <  3:63000/150‑1 (MQ=255)
        aCATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTg                                                                                                                                           <  4:20982/151‑1 (MQ=255)
          aTCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCt                                                                                                                                            <  2:1093089/148‑1 (MQ=255)
            cGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATg                                                                                                                                        <  3:150697/150‑1 (MQ=255)
               tACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCt                                                                                                                                    >  2:909994/1‑151 (MQ=255)
                 ccAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTAc                                                                                                                                  >  4:1557828/1‑151 (MQ=255)
                  cAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACg                                                                                                                                 <  2:1535782/151‑1 (MQ=255)
                   aGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGc                                                                                                                                >  3:146958/1‑151 (MQ=255)
                   aGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGc                                                                                                                                >  4:358230/1‑151 (MQ=255)
                    gAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGcc                                                                                                                               >  2:4972/1‑151 (MQ=255)
                    gAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGc                                                                                                                                <  4:1090571/150‑1 (MQ=255)
                          aTCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAAt                                                                                                                         >  1:455212/1‑151 (MQ=255)
                            cGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAAtt                                                                                                                        >  2:71818/1‑150 (MQ=255)
                             gTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTAc                                                                                                                      >  4:1545643/1‑151 (MQ=255)
                               ctcGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTAccc                                                                                                                    >  3:100667/1‑151 (MQ=255)
                                 cGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCtt                                                                                                                  <  4:138894/151‑1 (MQ=255)
                                   cTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTa                                                                                                                 >  2:1526916/1‑150 (MQ=255)
                                    ttGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAAt                                                                                                               >  2:1131452/1‑151 (MQ=255)
                                     tGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAAtg                                                                                                              >  4:1333813/1‑151 (MQ=255)
                                     tGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAAt                                                                                                               <  2:1002854/150‑1 (MQ=255)
                                      gCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAAtg                                                                                                              <  2:432432/150‑1 (MQ=255)
                                       ccGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAAtgtt                                                                                                            >  3:453757/1‑151 (MQ=255)
                                        cgcgTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAAtgttg                                                                                                           >  1:94474/1‑151 (MQ=255)
                                         gcgTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAAtgttgt                                                                                                          >  2:517947/1‑151 (MQ=255)
                                              tctGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTc                                                                                                     >  2:40630/1‑151 (MQ=255)
                                                 gCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGc                                                                                                  <  4:592857/151‑1 (MQ=255)
                                                      aacaacGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCgtggt                                                                                             <  2:661991/151‑1 (MQ=255)
                                                          acGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGcc                                                                                         <  2:63175/151‑1 (MQ=255)
                                                            gcgATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCgg                                                                                       <  1:100653/151‑1 (MQ=255)
                                                             cgATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGt                                                                                      <  1:427361/151‑1 (MQ=255)
                                                                tttAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTcc                                                                                   >  2:1185598/1‑151 (MQ=255)
                                                                  tAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGt                                                                                 >  3:205907/1‑151 (MQ=255)
                                                                     ccGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTAct                                                                              <  4:750041/151‑1 (MQ=255)
                                                                       gTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTActct                                                                            <  2:716758/151‑1 (MQ=255)
                                                                       gTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTActct                                                                            >  4:680324/1‑151 (MQ=255)
                                                                          ggTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGTCGGTTCCGTACTCTATg                                                                         >  4:1485447/1‑151 (MQ=255)
                                                                          ggTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTAt                                                                          <  4:865463/150‑1 (MQ=255)
                                                                           gTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATg                                                                         >  4:1249199/1‑150 (MQ=255)
                                                                              cAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACgg                                                                     <  3:94488/151‑1 (MQ=255)
                                                                                gCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGAc                                                                   >  3:265309/1‑151 (MQ=255)
                                                                                   cTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAAc                                                                >  4:346595/1‑151 (MQ=255)
                                                                                     cGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACt                                                               <  4:1323536/150‑1 (MQ=255)
                                                                                      ggCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACtt                                                              >  4:461627/1‑150 (MQ=255)
                                                                                      ggCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTa                                                             <  4:577723/151‑1 (MQ=255)
                                                                                       gCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTAAGACGGACAACTTAg                                                            <  4:1280267/151‑1 (MQ=255)
                                                                                          cAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTc                                                          <  4:803975/150‑1 (MQ=255)
                                                                                            aaCATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGc                                                        >  4:787082/1‑150 (MQ=255)
                                                                                            aaCATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCa                                                       <  4:720105/151‑1 (MQ=255)
                                                                                             aCATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGc                                                        >  4:328527/1‑149 (MQ=255)
                                                                                             aCATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAg                                                      <  4:1401113/151‑1 (MQ=255)
                                                                                             aCATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAg                                                      <  2:1092803/151‑1 (MQ=255)
                                                                                              cATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCGGc                                                     <  4:1220089/151‑1 (MQ=255)
                                                                                              cATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGc                                                     >  2:1044188/1‑151 (MQ=255)
                                                                                                ttATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCg                                                    >  4:478430/1‑150 (MQ=255)
                                                                                                ttATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCg                                                    >  1:107712/1‑150 (MQ=255)
                                                                                                 tATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGAt                                                  >  1:72706/1‑151 (MQ=255)
                                                                                                   tCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATgg                                                >  1:223831/1‑151 (MQ=255)
                                                                                                   tCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATgg                                                >  2:649362/1‑151 (MQ=255)
                                                                                                   tCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATg                                                 <  2:1035944/150‑1 (MQ=255)
                                                                                                      atatatTGCTGATCCTCGGTCGGGCTGCGGGGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGACgg                                                >  1:19070/1‑148 (MQ=255)
                                                                                                       tatatTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATc                                            >  4:700228/1‑151 (MQ=255)
                                                                                                         tatTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCtt                                          >  2:735053/1‑151 (MQ=255)
                                                                                                         tatTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCtt                                          >  2:1258274/1‑151 (MQ=255)
                                                                                                         tatTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCt                                           <  2:460978/150‑1 (MQ=255)
                                                                                                          atTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCttt                                         >  2:1617423/1‑151 (MQ=255)
                                                                                                            tGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTtct                                       >  4:269388/1‑151 (MQ=255)
                                                                                                            tGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTtc                                        <  4:40709/150‑1 (MQ=255)
                                                                                                                gATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTctcctc                                   >  4:1365903/1‑151 (MQ=255)
                                                                                                                gATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTctcctc                                   >  2:437293/1‑151 (MQ=255)
                                                                                                                gATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTctcct                                    <  4:1050874/150‑1 (MQ=255)
                                                                                                                gATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTctcc                                     <  4:545313/149‑1 (MQ=255)
                                                                                                                 aTCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCctct                                  >  4:749328/1‑151 (MQ=255)
                                                                                                                 aTCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCctct                                  <  2:1555903/151‑1 (MQ=255)
                                                                                                                  tCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCtt                                 >  4:15225/1‑151 (MQ=255)
                                                                                                                  tCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCtt                                 >  4:1677070/1‑151 (MQ=255)
                                                                                                                   ccTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCttt                                <  4:1140254/151‑1 (MQ=255)
                                                                                                                   ccTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCttt                                <  2:211656/151‑1 (MQ=255)
                                                                                                                   ccTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCtt                                 <  2:1644726/150‑1 (MQ=255)
                                                                                                                     tCGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCt                              >  2:133954/1‑151 (MQ=255)
                                                                                                                      cGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTg                             >  1:135502/1‑151 (MQ=255)
                                                                                                                      cGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTg                             >  2:1405635/1‑151 (MQ=255)
                                                                                                                      cGGTCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTg                             <  2:910826/151‑1 (MQ=255)
                                                                                                                         tCTGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCt                          >  4:1209129/1‑151 (MQ=255)
                                                                                                                           tGGCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCtgt                        >  2:291306/1‑151 (MQ=255)
                                                                                                                            ggCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCtgt                        <  4:1338301/150‑1 (MQ=255)
                                                                                                                             gCTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGc                      <  2:1419346/151‑1 (MQ=255)
                                                                                                                              cTGCGCTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCt                     >  4:1432353/1‑151 (MQ=255)
                                                                                                                                gcgcTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTAt                   <  4:870657/151‑1 (MQ=255)
                                                                                                                                 cgcTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATg                  <  1:405018/151‑1 (MQ=255)
                                                                                                                                  gcTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATgg                 <  2:1646233/151‑1 (MQ=255)
                                                                                                                                   cTGGTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGCGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATgg                 >  4:1646130/1‑150 (MQ=255)
                                                                                                                                     ggTTCGTCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGgc                <  4:1639959/149‑1 (MQ=255)
                                                                                                                                           tCCTTTTACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTc        >  4:747251/1‑151 (MQ=255)
                                                                                                                                              ttttACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCAtt     >  1:60769/1‑151 (MQ=255)
                                                                                                                                                 tACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGtt  >  1:244937/1‑151 (MQ=255)
                                                                                                                                                 tACAGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGt   <  2:1188792/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTCCGTACTCTATGACGGACAACTTAGTCGCAGCGATGGTATCTTTCTCCTCTTTCTGGCTGTGCTATGGCTGCTGTTCATTGTT  >  NC_000913/3340411‑3340706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: