Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,352,312 G→A R238R (CGC→CGT)  arcB ← aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,352,3120GA99.0% 406.6 / NA 103R238R (CGC→CGT) arcBaerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (42/60);  minor base C (1/0);  total (43/60)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

AGATAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTGCGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATCAGCAGGTTTCAGGTGAACCAGTTGTTTTTCGCTTTTT  >  NC_000913/3352164‑3352459
                                                                                                                                                    |                                                                                                                                                   
agaTAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTacg                                                                                                                                                   <  3:432628/151‑1 (MQ=255)
  aTAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGcc                                                                                                                                                 <  4:1417269/151‑1 (MQ=255)
  aTAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGcc                                                                                                                                                 <  4:266827/151‑1 (MQ=255)
    aaaCGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGccc                                                                                                                                                <  4:170177/150‑1 (MQ=255)
    aaaCGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCg                                                                                                                                               >  4:1194681/1‑151 (MQ=255)
     aaCGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGt                                                                                                                                              >  4:781946/1‑151 (MQ=255)
     aaCGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGt                                                                                                                                              <  4:1312933/151‑1 (MQ=255)
       cgtcgtTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTcc                                                                                                                                            <  4:1450539/151‑1 (MQ=255)
       cgtcgtTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTcc                                                                                                                                            <  4:424213/151‑1 (MQ=255)
        gtcgtTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCg                                                                                                                                           <  2:54248/151‑1 (MQ=255)
          cgtTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCggg                                                                                                                                         >  1:325320/1‑151 (MQ=255)
           gtTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGt                                                                                                                                        <  1:258568/151‑1 (MQ=255)
               tGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATc                                                                                                                                    >  2:409650/1‑151 (MQ=255)
                  cgcgGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCa                                                                                                                                  <  2:636877/150‑1 (MQ=255)
                   gcgGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGc                                                                                                                                >  2:1093936/1‑151 (MQ=255)
                     ggCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGcc                                                                                                                               >  2:759165/1‑150 (MQ=255)
                         ggCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGt                                                                                                                          >  4:1113354/1‑151 (MQ=255)
                         ggCCCGTTCAAGCGCATCCTGACACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGt                                                                                                                          <  1:311575/151‑1 (MQ=255)
                          gCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGtt                                                                                                                         <  3:451359/151‑1 (MQ=255)
                            ccGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCa                                                                                                                       <  2:634394/151‑1 (MQ=255)
                            ccGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCa                                                                                                                       <  2:880840/151‑1 (MQ=255)
                              gTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATa                                                                                                                     <  3:435913/151‑1 (MQ=255)
                                tCAAGCGCATCCTGATGCCGCTTACCCTCGGTAATGTCGCGACCAAAGCCCATCAAACCCTGCCGTTTACCCACGCGGTCGTAGTACGGCACTTTCCGGGTTTCAAAGCAGGCTTTCCGCCCGTCCGCGCAATCCAGCCACTGTTCATa                                                                                                                     >  2:832602/1‑149 (MQ=255)
                                tCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAg                                                                                                                    <  2:1070868/150‑1 (MQ=255)
                                  aaGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTc                                                                                                                 >  3:374156/1‑151 (MQ=255)
                                    gcgcATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTc                                                                                                                 >  4:1235902/1‑149 (MQ=255)
                                    gcgcATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAg                                                                                                               <  1:414451/151‑1 (MQ=255)
                                      gcATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTg                                                                                                             <  4:630623/151‑1 (MQ=255)
                                      gcATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTg                                                                                                             >  2:787126/1‑151 (MQ=255)
                                       cATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTg                                                                                                             >  2:79761/1‑150 (MQ=255)
                                             gATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGAcaca                                                                                                        <  4:514502/149‑1 (MQ=255)
                                             gATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACAtt                                                                                                      <  3:162046/151‑1 (MQ=255)
                                             gATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACAtt                                                                                                      <  2:699187/151‑1 (MQ=255)
                                                aCCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTAt                                                                                                    <  4:30455/150‑1 (MQ=255)
                                                aCCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATg                                                                                                   <  1:122310/151‑1 (MQ=255)
                                                 ccGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATg                                                                                                   <  4:1559776/150‑1 (MQ=255)
                                                  cGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGAc                                                                                                 <  2:50773/151‑1 (MQ=255)
                                                    cTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACg                                                                                                <  2:1284276/150‑1 (MQ=255)
                                                      tACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACgg                                                                                               <  2:982098/149‑1 (MQ=255)
                                                      tACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACgg                                                                                               <  4:1006397/149‑1 (MQ=255)
                                                       aCGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGaa                                                                                             <  2:257601/150‑1 (MQ=255)
                                                        cGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAac                                                                                            >  4:213332/1‑150 (MQ=255)
                                                         gCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAacac                                                                                          <  4:279080/151‑1 (MQ=255)
                                                           tCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACtt                                                                                        >  4:593587/1‑151 (MQ=255)
                                                           tCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACtt                                                                                        >  1:453430/1‑151 (MQ=255)
                                                            cGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACtt                                                                                        <  4:1649684/150‑1 (MQ=255)
                                                             ggTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACtttt                                                                                      <  2:1018414/151‑1 (MQ=255)
                                                             ggTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACtttt                                                                                      >  3:285906/1‑151 (MQ=255)
                                                               tAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTc                                                                                    >  2:365248/1‑151 (MQ=255)
                                                                aaTGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTc                                                                                    >  2:1571910/1‑150 (MQ=255)
                                                                aaTGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCa                                                                                   <  3:268770/151‑1 (MQ=255)
                                                                 aTGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCAt                                                                                  >  4:475251/1‑151 (MQ=255)
                                                                 aTGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCAt                                                                                  >  2:1423186/1‑151 (MQ=255)
                                                                   gTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCg                                                                                <  2:59501/151‑1 (MQ=255)
                                                                   gTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCg                                                                                <  4:81334/151‑1 (MQ=255)
                                                                     cgcgACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCgga                                                                              <  2:895141/151‑2 (MQ=255)
                                                                     cgcgACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCgg                                                                               <  1:320275/150‑1 (MQ=255)
                                                                     cgcgACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGt                                                                              <  3:8087/151‑1 (MQ=255)
                                                                      gcgACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGt                                                                              <  4:1436086/150‑1 (MQ=255)
                                                                       tgACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGttt                                                                            >  1:216850/2‑151 (MQ=255)
                                                                       cgACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGCACACTTTTTCATCGGttt                                                                            >  1:135760/1‑151 (MQ=255)
                                                                           cAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAAt                                                                        <  3:146187/151‑1 (MQ=255)
                                                                           cAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAAt                                                                        >  2:834077/1‑151 (MQ=255)
                                                                                cccATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACtt                                                                   <  2:1089860/151‑1 (MQ=255)
                                                                                 ccATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACttt                                                                  <  2:780798/151‑1 (MQ=255)
                                                                                    tCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTgc                                                               >  2:550761/1‑151 (MQ=255)
                                                                                    tCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTg                                                                >  2:970481/1‑150 (MQ=255)
                                                                                     cAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTgcg                                                              >  1:321359/1‑151 (MQ=255)
                                                                                     cAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTgc                                                               <  4:1553701/150‑1 (MQ=255)
                                                                                      aaaCCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTgcgg                                                             <  3:308788/151‑1 (MQ=255)
                                                                                      aaaCCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTgcgg                                                             <  1:179168/151‑1 (MQ=255)
                                                                                         ccGTGACGTTTACCCACGCGGTCGTAATACGGCACTTTACGGATTTCAAAGAAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTgcggcga                                                          <  1:447513/151‑2 (MQ=255)
                                                                                             gACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCtt                                                       <  2:1608405/150‑1 (MQ=255)
                                                                                             gACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTc                                                      >  3:167433/1‑151 (MQ=255)
                                                                                                gTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCgg                                                   >  2:118858/1‑151 (MQ=255)
                                                                                                 tttACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCgg                                                   >  2:491909/1‑150 (MQ=255)
                                                                                                  ttACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGt                                                  <  4:94620/150‑1 (MQ=255)
                                                                                                  ttACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTg                                                 >  3:26325/1‑151 (MQ=255)
                                                                                                    aCCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTg                                                 >  4:757433/1‑149 (MQ=255)
                                                                                                       cACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTaa                                            <  3:65935/151‑1 (MQ=255)
                                                                                                           cgGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACAt                                        <  2:429586/151‑1 (MQ=255)
                                                                                                           cgGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGGGAGTaaa                                           >  4:1131255/1‑148 (MQ=255)
                                                                                                             gTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATc                                       >  2:299584/1‑150 (MQ=255)
                                                                                                               cGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATcagc                                    >  2:783837/1‑151 (MQ=255)
                                                                                                               cGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATcagc                                    <  4:410236/151‑1 (MQ=255)
                                                                                                                        gCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATCAGCAGGTTTCAg                           <  2:780919/151‑1 (MQ=255)
                                                                                                                        gCACTTTACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATCAGCAGGTTTCAg                           <  1:247705/151‑1 (MQ=255)
                                                                                                                             ttACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATCAGCAGGTTTCAGGTGa                       >  3:46111/1‑150 (MQ=255)
                                                                                                                              tACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATCAGCAGGTTTCAGGTGaa                      <  2:1616519/150‑1 (MQ=255)
                                                                                                                              tACGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATCAGCAGGTTTCAGGTGAAc                     >  2:879812/1‑151 (MQ=255)
                                                                                                                               aCGGATTTCAAAGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATCAGCAGGTTTCAGGTGAAc                     <  4:287029/150‑1 (MQ=255)
                                                                                                                                          aGCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATCAGCAGGTTTCAGGTGAACCAGTTGTTTTTc         <  2:339350/151‑1 (MQ=255)
                                                                                                                                           gCAGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATCAGCAGGTTTCAGGTGAACCAGTTGTTTTTc         <  4:1362600/150‑1 (MQ=255)
                                                                                                                                             aGGCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATCAGCAGGTTTCAGGTGAACCAGTTGTTTTTCGCt      >  2:1611238/1‑151 (MQ=255)
                                                                                                                                              ggCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATCAGCAGGTTTCAGGTGAACCAGTTGTTTTTCGCtt     >  4:232021/1‑151 (MQ=255)
                                                                                                                                              ggCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATCAGCAGGTTTCAGGTGAACCAGTTGTTTTTCGCtt     >  2:822433/1‑151 (MQ=255)
                                                                                                                                              ggCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATCAGCAGGTTTCAGGTGAACCAGTTGTTTTTCGCtt     <  4:85126/151‑1 (MQ=255)
                                                                                                                                              ggCTTTACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATCAGCAGGTTTCAGGTGAACCAGTTGTTTTTCGCtt     >  3:294329/1‑151 (MQ=255)
                                                                                                                                                 tttACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATCAGCAGGTTTCAGGTGAACCAGTTGTTTTTCGCttttt  >  3:478472/1‑151 (MQ=255)
                                                                                                                                                 tttACGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATCAGCAGGTTTCAGGTGAACCAGTTGTTTTTCGCttttt  >  2:100729/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AGATAAACGTCGTTTTGTCGCGGCTGGCCCGTTCAAGCGCATCCTGATACCGCTTACGCTCGGTAATGTCGCGACCAAAGCCCATCAAACCGTGACGTTTACCCACGCGGTCGTAGTACGGCACTTTACGGATTTCAAAGCAGGCTTTGCGCCCGTCCGGGTAATCCAGCCACTGTTCATAGGTCAGTGACACATTATGACGGAACACTTTTTCATCGGTTTCAATGACTTTTGCGGCGGCTTCCGGTGAGTAAACATCAGCAGGTTTCAGGTGAACCAGTTGTTTTTCGCTTTTT  >  NC_000913/3352164‑3352459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑