BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,523,544	T→C	V225A (GTG→GCG)	mrcA →	penicillin‑binding protein 1a, murein transglycosylase and transpeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,523,544	0	T	C	100.0%	387.0 / NA	113	V225A (GTG→GCG)	mrcA	penicillin‑binding protein 1a, murein transglycosylase and transpeptidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (54/59); total (54/59)

Only 100 of 116 total aligned reads displayed.

GTCGGTGCTGCGGCACAAGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGTGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTAT  >  NC_000913/3523396‑3523689

gtCGGTGCTGCGGCACAAGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCgcgc                                                                                                                                                 <  2:1558651/151‑1 (MQ=255)

    gTGCTGCGGCACAAGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTc                                                                                                                                             <  2:748259/151‑1 (MQ=255)

    gTGCTGCGGCACAAGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTc                                                                                                                                             <  3:163900/151‑1 (MQ=255)

      gctgcGGCACAAGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTcgc                                                                                                                                           <  2:868959/151‑1 (MQ=255)

         gcGGCACAAGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTcgcg                                                                                                                                          <  1:361634/149‑1 (MQ=255)

          cGGCACAAGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGAt                                                                                                                                       <  4:1173128/151‑1 (MQ=255)

             cacaAGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCCCCTTCAAACCGCTCTACTCGATGGGTCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCgg                                                                                                                                         >  2:417501/1‑146 (MQ=255)

              acaAGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGAGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGGGGAAAGTCGCGCTGTCGCGGATGCTg                                                                                                                                   >  3:12151/1‑151 (MQ=255)

               caAGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTgg                                                                                                                                  <  4:736171/151‑1 (MQ=255)

                aaGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCCACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGGAAAGTCGCGCTGTCGCGGGTGCTgg                                                                                                                                  >  2:1071976/1‑150 (MQ=255)

                aaGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCCCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTgg                                                                                                                                  >  4:463549/1‑150 (MQ=255)

                aaGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCCTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGGTGCTgg                                                                                                                                  >  2:764025/1‑150 (MQ=255)

                      aTTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAg                                                                                                                            <  2:873568/150‑1 (MQ=255)

                      aTTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAg                                                                                                                            <  1:355943/150‑1 (MQ=255)

                      aTTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAg                                                                                                                            <  1:33076/150‑1 (MQ=255)

                           ggAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGgat                                                                                                                       >  4:131087/1‑150 (MQ=255)

                            gAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTatat                                                                                                                     <  1:457083/151‑1 (MQ=255)

                             aaaaaCGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTatat                                                                                                                     <  4:82701/150‑1 (MQ=255)

                             aaaaaCGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAAACCGCCCTACTCGATGGGTCGTGCCGTCGCGCGGCGGAAAGTCGCGCTGTCGCGGATGCTGGGTGAAGGGgata                                                                                                                      >  2:1107003/1‑149 (MQ=255)

                                aaCGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAACCGGGGTGCCGAAAGCGCCCTCCACCCTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGGATATCAcc                                                                                                                 >  2:62668/1‑151 (MQ=255)

                                  cGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCCGGGCGCCGGAAACGCCCTCCACCTTTAACCCGCTCTACTCGATGGATTGTGCCGTCGAGGGGGGTAACGTCGCGGTGTCGCCGACGCTGGGTGGAGGGGATATCAccc                                                                                                                >  2:1411976/1‑150 (MQ=255)

                                   ggTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGGAACGTCGCGCTGTCGCGGATGCTGGGTGAAGGGGATATCAccc                                                                                                                >  4:1146436/1‑149 (MQ=255)

                                   ggTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCCTCCACCCTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGGTGCTGGATGAAGGGGATATCACCca                                                                                                               >  2:904519/1‑150 (MQ=255)

                                     tCGACCACCTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCcaa                                                                                                              <  4:540619/149‑1 (MQ=255)

                                      cGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCcaacaa                                                                                                           <  4:1122682/151‑1 (MQ=255)

                                       gACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGGATATCACCcaacaa                                                                                                           >  4:1641617/1‑150 (MQ=255)

                                         ccAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGAGCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAg                                                                                                        <  2:1456253/151‑1 (MQ=255)

                                               gACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCTCCCAACAACAGTTCg                                                                                                    <  2:1273056/149‑1 (MQ=255)

                                               gACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCg                                                                                                    <  2:1253834/149‑1 (MQ=255)

                                               gACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCCTCCACCTTCAACCCGCTCTACTCGATGGGTCGTGCCGTCGCGCGGCGTAAAGTCGCGCTGTCGCGGAGGCTGGATGAAGGGGATATCACCCAACAACAGTTCGAt                                                                                                  >  2:248979/1‑151 (MQ=255)

                                                 cGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCCTCGCGCGGCGTAACGTCGCGCTGTCGCGGGTGCTGGATGAAGGGGATATCACCCAACAACAGGTCg                                                                                                    >  1:47198/1‑147 (MQ=255)

                                                  gCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAg                                                                                               <  2:407803/151‑1 (MQ=255)

                                                           aaTGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACt                                                                                      <  4:183432/151‑1 (MQ=255)

                                                              ggCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTg                                                                                     >  2:945338/1‑149 (MQ=255)

                                                              ggCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGa                                                                                    <  4:452153/150‑1 (MQ=255)

                                                              ggCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAg                                                                                   <  4:1044626/151‑1 (MQ=255)

                                                                cGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCCCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGc                                                                                 >  3:469919/1‑151 (MQ=255)

                                                                 ggTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCg                                                                                <  1:75721/151‑1 (MQ=255)

                                                                   tGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGAt                                                                              >  3:288107/1‑151 (MQ=255)

                                                                   tGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGAt                                                                              >  2:1284803/1‑151 (MQ=255)

                                                                    gATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGAtt                                                                             >  2:253310/1‑151 (MQ=255)

                                                                    gATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGAtt                                                                             >  2:1089648/1‑151 (MQ=255)

                                                                    gATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGAt                                                                              <  4:1465425/150‑1 (MQ=255)

                                                                    gATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGAt                                                                              <  4:592240/150‑1 (MQ=255)

                                                                     aTAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTa                                                                            <  4:1277072/151‑1 (MQ=255)

                                                                      tAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGAtt                                                                             <  4:452739/149‑1 (MQ=255)

                                                                         ccGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGc                                                                        <  4:765045/151‑1 (MQ=255)

                                                                          cGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCt                                                                       >  2:1351065/1‑151 (MQ=255)

                                                                            ggCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTa                                                                      <  2:1481839/150‑1 (MQ=255)

                                                                                gCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACt                                                                   <  2:1732/149‑1 (MQ=255)

                                                                                gCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTAt                                                                 >  4:161501/1‑151 (MQ=255)

                                                                                gCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTAt                                                                 <  2:1032943/151‑1 (MQ=255)

                                                                                  cGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATc                                                                >  4:164191/1‑150 (MQ=255)

                                                                                    aaaGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCAc                                                              >  4:1581635/1‑150 (MQ=255)

                                                                                     aaGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCAcgc                                                            <  3:461316/151‑1 (MQ=255)

                                                                                     aaGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCAcgc                                                            <  1:469776/151‑1 (MQ=255)

                                                                                      tgcgcCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCAcgcg                                                           >  2:584497/2‑151 (MQ=255)

                                                                                       gcgcCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACgcgc                                                          >  3:374888/1‑151 (MQ=255)

                                                                                       gcgcCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACgcgc                                                          <  4:1073341/151‑1 (MQ=255)

                                                                                           cTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCgg                                                       >  2:914179/1‑150 (MQ=255)

                                                                                            ttCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCgg                                                       >  2:1086569/1‑149 (MQ=255)

                                                                                             tCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGgag                                                     <  2:1663371/150‑1 (MQ=255)

                                                                                             tCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGga                                                      <  2:995862/149‑1 (MQ=255)

                                                                                               cACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGAtt                                                  >  4:805549/1‑151 (MQ=255)

                                                                                                  cTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCt                                               >  2:49805/1‑151 (MQ=255)

                                                                                                   ttCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCtt                                              >  2:1321940/1‑151 (MQ=255)

                                                                                                    tCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCttt                                             >  4:1005565/1‑151 (MQ=255)

                                                                                                        cccGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTctct                                         <  3:415472/151‑1 (MQ=255)

                                                                                                         ccGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTg                                        <  2:107374/151‑1 (MQ=255)

                                                                                                             tctACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGcc                                    >  2:1143492/1‑151 (MQ=255)

                                                                                                              ctACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCg                                   >  2:756432/1‑151 (MQ=255)

                                                                                                               tACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGt                                  >  3:189096/1‑151 (MQ=255)

                                                                                                                aCTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGAGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTACTCTGCGCCGt                                  <  1:255537/150‑1 (MQ=255)

                                                                                                                   cGATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCt                              >  4:109120/1‑151 (MQ=255)

                                                                                                                    gATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTg                             <  3:401339/151‑1 (MQ=255)

                                                                                                                    gATGGATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTg                             >  1:402175/1‑151 (MQ=255)

                                                                                                                       ggATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGc                          <  4:1025046/151‑1 (MQ=255)

                                                                                                                        gATCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCg                         <  4:68432/151‑1 (MQ=255)

                                                                                                                         aTCGTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGa                        <  1:70681/151‑1 (MQ=255)

                                                                                                                            gTGCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAAt                     >  2:1403316/1‑151 (MQ=255)

                                                                                                                              gCCGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATgg                   <  4:222240/151‑1 (MQ=255)

                                                                                                                                cGTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTg                 <  1:143791/151‑1 (MQ=255)

                                                                                                                                 gTCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTgc                >  4:1114116/1‑151 (MQ=255)

                                                                                                                                  tCGCGCGGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTgcg               >  4:641094/1‑151 (MQ=255)

                                                                                                                                   cgcgcgGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTgcgc              <  4:1537495/151‑1 (MQ=255)

                                                                                                                                   cgcgcgGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTgcg               <  2:805471/150‑1 (MQ=255)

                                                                                                                                    gcgcgGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGcc             >  3:96701/1‑151 (MQ=255)

                                                                                                                                     cgcgGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGcc             >  2:811407/1‑150 (MQ=255)

                                                                                                                                     cgcgGCGTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCa            <  1:173012/151‑1 (MQ=255)

                                                                                                                                      gcggcgTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGcc             >  2:117427/1‑149 (MQ=255)

                                                                                                                                      gcggcgTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGcc             >  4:993161/1‑149 (MQ=255)

                                                                                                                                          cgTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAgg          >  2:333865/1‑148 (MQ=255)

                                                                                                                                          cgTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGgaga       <  2:239409/151‑1 (MQ=255)

                                                                                                                                          cgTAACGTCGCGCTGTCGCGGATGCTGGAGGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGTTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGgag        <  4:253715/150‑1 (MQ=255)

                                                                                                                                           gTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAgg          >  2:676705/1‑147 (MQ=255)

                                                                                                                                           gTAACGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGAt      <  4:1626190/151‑1 (MQ=255)

                                                                                                                                             aaCGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATg     <  4:1331869/150‑1 (MQ=255)

                                                                                                                                             aaCGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGt    <  2:1444501/151‑1 (MQ=255)

                                                                                                                                              aCGTCGCGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGta   <  2:142383/151‑1 (MQ=255)

                                                                                                                                               agtcgcGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGtat  >  2:397032/2‑151 (MQ=255)

GTCGGTGCTGCGGCACAAGTCTATTTCGGAAAAACGGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGCTGCCGAAAGCGCCTTCCACCTTCAACCCGCTCTACTCGATGGATCGTGCCGTCGCGCGGCGTAACGTCGTGCTGTCGCGGATGCTGGATGAAGGGTATATCACCCAACAACAGTTCGATCAGACACGCACTGAGGCGATTAACGCTAACTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAATGGTGCGCCAGGAGATGTAT  >  NC_000913/3523396‑3523689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`