Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,550,505 T→C T657A (ACG→GCG)  malP ← maltodextrin phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,550,5050TC100.0% 426.4 / NA 116T657A (ACG→GCG) malPmaltodextrin phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (73/43);  total (73/43)

Only 100 of 116 total aligned reads displayed.

GCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCA  >  NC_000913/3550357‑3550651
                                                                                                                                                    |                                                                                                                                                  
gCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCAc                                                                                                                                                  <  2:1298844/151‑1 (MQ=255)
  gTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCg                                                                                                                                                <  1:124361/151‑1 (MQ=255)
          ccTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTa                                                                                                                                          >  2:1408553/1‑149 (MQ=255)
           cTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAgc                                                                                                                                       >  4:122238/1‑151 (MQ=255)
            ttATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAgcg                                                                                                                                      >  3:116485/1‑151 (MQ=255)
             tATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTCGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAgc                                                                                                                                       <  4:184939/149‑1 (MQ=255)
               tctttcttCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAgcg                                                                                                                                      >  2:1329836/1‑148 (MQ=255)
                 tttcttCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCAcc                                                                                                                                  >  4:303696/1‑150 (MQ=255)
                 tttcttCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCAcc                                                                                                                                  >  4:1454917/1‑150 (MQ=255)
                  ttcttcCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCa                                                                                                                                    <  4:190920/147‑1 (MQ=255)
                  ttcttcCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCAc                                                                                                                                   <  4:154193/148‑1 (MQ=255)
                  ttcttcCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCAt                                                                                                                                >  1:287880/1‑151 (MQ=255)
                   tcttcCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCAtt                                                                                                                               >  4:961323/1‑151 (MQ=255)
                   tcttcCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCAtt                                                                                                                               >  3:320187/1‑151 (MQ=255)
                     ttcCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTg                                                                                                                              >  4:1183183/1‑150 (MQ=255)
                      tcCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCAtt                                                                                                                               <  1:364328/148‑1 (MQ=255)
                      tcCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGa                                                                                                                             <  4:534416/150‑1 (MQ=255)
                        cGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAgcg                                                                                                                          >  4:8737/1‑151 (MQ=255)
                        cGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAgcg                                                                                                                          >  4:140732/1‑151 (MQ=255)
                           cATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGcc                                                                                                                        >  3:212634/1‑150 (MQ=255)
                              ttCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGtt                                                                                                                    >  1:400524/1‑151 (MQ=255)
                               tCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGtt                                                                                                                    <  2:1393334/150‑1 (MQ=255)
                                cACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGttt                                                                                                                   <  4:382097/150‑1 (MQ=255)
                                cACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTc                                                                                                                  >  2:1381745/1‑151 (MQ=255)
                                 aCCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTc                                                                                                                  >  2:978276/1‑150 (MQ=255)
                                  ccGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCat                                                                                                                <  4:777476/151‑1 (MQ=255)
                                       tCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGc                                                                                                           >  2:501019/1‑151 (MQ=255)
                                        cGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGcc                                                                                                          <  3:201240/151‑1 (MQ=255)
                                            gCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGt                                                                                                       >  2:1014438/1‑150 (MQ=255)
                                              cTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTAcc                                                                                                    >  2:445438/1‑151 (MQ=255)
                                                ttGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTAcc                                                                                                    <  1:105769/149‑1 (MQ=255)
                                                 tGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCgg                                                                                                  >  2:948704/1‑150 (MQ=255)
                                                  ggCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGaa                                                                                                <  2:374709/151‑1 (MQ=255)
                                                  ggCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGa                                                                                                 <  1:396795/150‑1 (MQ=255)
                                                     cAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGct                                                                                             >  3:207752/1‑151 (MQ=255)
                                                       gAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGcttc                                                                                           >  4:426305/1‑151 (MQ=255)
                                                       gAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGcttc                                                                                           >  2:547487/1‑151 (MQ=255)
                                                        aaTTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGcttct                                                                                          >  1:391781/1‑151 (MQ=255)
                                                        aaTTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGcttc                                                                                           <  2:1579974/150‑1 (MQ=255)
                                                         aTTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGcttctt                                                                                         >  2:427808/1‑151 (MQ=255)
                                                            gCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTAc                                                                                      >  4:1165343/1‑151 (MQ=255)
                                                            gCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTAc                                                                                      >  1:418489/1‑151 (MQ=255)
                                                             ccTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTAcc                                                                                     >  4:1467466/1‑151 (MQ=255)
                                                                 cACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGc                                                                                  >  2:185246/1‑150 (MQ=255)
                                                                 cACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGc                                                                                  <  1:219945/150‑1 (MQ=255)
                                                                 cACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCa                                                                                 <  2:647776/151‑1 (MQ=255)
                                                                     tGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTc                                                                              <  4:1530459/150‑1 (MQ=255)
                                                                         ccACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAAt                                                                         <  4:42632/151‑1 (MQ=255)
                                                                          cACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAAtt                                                                        <  4:581822/151‑1 (MQ=255)
                                                                          cACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAAtt                                                                        >  3:159116/1‑151 (MQ=255)
                                                                             ggTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGt                                                                     <  2:1185007/151‑1 (MQ=255)
                                                                             ggTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGt                                                                     >  4:1498769/1‑151 (MQ=255)
                                                                              gTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGtt                                                                    >  4:295492/1‑151 (MQ=255)
                                                                                  gACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCgg                                                                 >  4:1052503/1‑150 (MQ=255)
                                                                                   aCCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCgg                                                                 >  4:170000/1‑149 (MQ=255)
                                                                                   aCCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCgg                                                                 >  4:1234044/1‑149 (MQ=255)
                                                                                      aaaaaTAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGat                                                             >  3:447867/1‑150 (MQ=255)
                                                                                       aaaaTAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGatat                                                           >  2:1571180/1‑151 (MQ=255)
                                                                                                atatTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATccgccgccg                                                  <  4:1290959/151‑1 (MQ=255)
                                                                                                atatTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCACATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATccgccgccg                                                  >  2:1386499/1‑151 (MQ=255)
                                                                                                   ttttCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTAAGCGCCAGTTTCATATTACCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATccgccgtc                                                   >  2:421502/1‑145 (MQ=255)
                                                                                                    tttCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGAt                                              >  4:761463/1‑151 (MQ=255)
                                                                                                    tttCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGAt                                              >  2:124007/1‑151 (MQ=255)
                                                                                                     ttcttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATc                                             >  2:852805/1‑151 (MQ=255)
                                                                                                     ttcttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATc                                             >  3:332481/1‑151 (MQ=255)
                                                                                                       cttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATc                                             >  4:7680/1‑149 (MQ=255)
                                                                                                        ttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGt                                          >  2:295066/1‑151 (MQ=255)
                                                                                                         tcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGtt                                         >  4:1668661/1‑151 (MQ=255)
                                                                                                          cACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGttt                                        <  2:7665/151‑1 (MQ=255)
                                                                                                           aCCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGtttt                                       >  3:152140/1‑151 (MQ=255)
                                                                                                           aCCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGtttt                                       >  2:2351/1‑151 (MQ=255)
                                                                                                           aCCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGtttt                                       >  4:784386/1‑151 (MQ=255)
                                                                                                            ccGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGtttt                                       <  4:1023532/150‑1 (MQ=255)
                                                                                                             cGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTc                                     <  1:287979/151‑1 (MQ=255)
                                                                                                             cGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTc                                     >  1:34119/1‑151 (MQ=255)
                                                                                                              gACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTcc                                    >  3:430332/1‑151 (MQ=255)
                                                                                                              gACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTcc                                    >  2:912575/1‑151 (MQ=255)
                                                                                                                  ttCTCGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTccgcc                                 <  4:252392/150‑1 (MQ=255)
                                                                                                                   tctcGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTccgccgc                               >  3:115456/1‑151 (MQ=255)
                                                                                                                   tctcGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGCACCGGAAGCTTCTTTACCTGCCGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTccgccgc                               >  1:440743/1‑151 (MQ=255)
                                                                                                                      cGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGGAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTccgccgc                               <  4:205160/148‑1 (MQ=255)
                                                                                                                      cGGCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGa                            <  3:41780/151‑1 (MQ=255)
                                                                                                                       ggCGATTTCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGaa                           <  3:379583/151‑1 (MQ=255)
                                                                                                                             ttCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCa                      <  2:944070/150‑1 (MQ=255)
                                                                                                                              tCAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCaa                     <  2:1650423/150‑1 (MQ=255)
                                                                                                                               cAACGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCaat                    >  2:611894/1‑150 (MQ=255)
                                                                                                                                aaCGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCaataa                  <  2:190447/151‑1 (MQ=255)
                                                                                                                                aaCGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCaata                   >  2:482362/1‑150 (MQ=255)
                                                                                                                                 aCGTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCaataat                 >  3:268523/1‑151 (MQ=255)
                                                                                                                                   gTTCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATcc               >  2:160830/1‑151 (MQ=255)
                                                                                                                                    ttCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCg              >  2:661117/1‑151 (MQ=255)
                                                                                                                                    ttCGCCCCATACAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCg              >  4:1465328/1‑151 (MQ=255)
                                                                                                                                     tCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTGCCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCg              <  4:151312/150‑1 (MQ=255)
                                                                                                                                     tCGCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCgg             >  3:298500/1‑151 (MQ=255)
                                                                                                                                       gCCCCATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCa           <  4:1410315/151‑1 (MQ=255)
                                                                                                                                        ccccATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGc            >  1:76200/1‑149 (MQ=255)
                                                                                                                                         cccATCCAGCGCACCGGCAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTGCCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCGATAATCCGGCAgg         <  4:276746/151‑1 (MQ=255)
                                                                                                                                           cATCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGaa       <  1:430314/151‑1 (MQ=255)
                                                                                                                                             tCCAGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAac      >  2:1047946/1‑150 (MQ=255)
                                                                                                                                                aGCGCACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAAcacca  <  4:501722/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCGTCCAGCACCTTATCTTTCTTCCGCCATTTCACCGGGTCGTAGCCTTTGGCCAGAATTGCCTTCACTTGTTCCACGGTATGACCAAAAATAAAGATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCA  >  NC_000913/3550357‑3550651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: