Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 379,275 A→G R99R (CGT→CGC)  frmA ← alcohol dehydrogenase class III; glutathione‑dependent formaldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913379,2750AG99.1% 417.0 / ‑4.4 110R99R (CGT→CGC) frmAalcohol dehydrogenase class III; glutathione‑dependent formaldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (53/56);  total (54/56)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 112 total aligned reads displayed.

CGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCGGGAATACACCTTCCGGGTCA  >  NC_000913/379128‑379422
                                                                                                                                                   |                                                                                                                                                   
cgcgACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCg                                                                                                                                                   >  2:1651257/1‑150 (MQ=255)
cgcgACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGa                                                                                                                                                  <  2:1545783/151‑1 (MQ=255)
 tcgACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGAc                                                                                                                                                 >  2:320237/2‑151 (MQ=255)
  cgACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACa                                                                                                                                                <  4:790015/151‑1 (MQ=255)
         ggTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTc                                                                                                                                          >  4:518873/1‑150 (MQ=255)
         ggTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTc                                                                                                                                          >  4:374399/1‑150 (MQ=255)
          gtgtATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTcac                                                                                                                                        >  4:951570/1‑151 (MQ=255)
                 cACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTcgcc                                                                                                                                 <  4:462757/151‑1 (MQ=255)
                     gAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCa                                                                                                                              <  1:262858/150‑1 (MQ=255)
                     gAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCAc                                                                                                                             >  4:620282/1‑151 (MQ=255)
                       aTGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTc                                                                                                                           <  2:205541/151‑1 (MQ=255)
                       aTGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTc                                                                                                                           >  4:1033354/1‑151 (MQ=255)
                       aTGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTc                                                                                                                           <  4:1103454/151‑1 (MQ=255)
                        tGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTcc                                                                                                                          >  2:937494/1‑151 (MQ=255)
                        tGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTcc                                                                                                                          >  4:460535/1‑151 (MQ=255)
                         gTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTcc                                                                                                                          <  3:398448/150‑1 (MQ=255)
                         gTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCcg                                                                                                                         <  2:472975/151‑1 (MQ=255)
                           agagCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCcgcg                                                                                                                       >  4:1014410/1‑151 (MQ=255)
                           agagCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCcgcg                                                                                                                       >  4:1000039/1‑151 (MQ=255)
                           agagCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCcgcg                                                                                                                       <  4:726780/151‑1 (MQ=255)
                           agagCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCcgcg                                                                                                                       >  4:924871/1‑151 (MQ=255)
                            gagCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCgg                                                                                                                      >  2:844870/1‑151 (MQ=255)
                                aCCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGgtgt                                                                                                                   >  4:1539621/1‑150 (MQ=255)
                                     aTGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCg                                                                                                             <  4:1630141/151‑1 (MQ=255)
                                     aTGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCg                                                                                                             >  2:267161/1‑151 (MQ=255)
                                       gTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCggg                                                                                                           <  4:374197/151‑1 (MQ=255)
                                          gTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATc                                                                                                        >  4:1180010/1‑151 (MQ=255)
                                           tGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATc                                                                                                        >  2:853365/1‑150 (MQ=255)
                                             aTAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATcaca                                                                                                     <  3:392299/151‑1 (MQ=255)
                                               aaaGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATg                                                                                                   <  2:330592/151‑1 (MQ=255)
                                                aaGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATgg                                                                                                  <  2:39355/151‑1 (MQ=255)
                                                   cGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCg                                                                                               >  4:1479453/1‑151 (MQ=255)
                                                    ggCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGc                                                                                              >  4:1603500/1‑151 (MQ=255)
                                                    ggCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGc                                                                                              <  4:791665/151‑1 (MQ=255)
                                                     gCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGcc                                                                                             >  1:293652/1‑151 (MQ=255)
                                                        gCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAgg                                                                                          <  3:295834/151‑1 (MQ=255)
                                                        gCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAAAACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCa                                                                                            <  4:1228471/149‑1 (MQ=255)
                                                         cccGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAgg                                                                                          <  4:1056520/150‑1 (MQ=255)
                                                         cccGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGt                                                                                         <  2:225884/151‑1 (MQ=255)
                                                          ccGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGTGGTGTAAAGCGGGATCACATGGTCGCCAGGtt                                                                                        >  2:1267101/1‑151 (MQ=255)
                                                          ccGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGtt                                                                                        <  1:441002/151‑1 (MQ=255)
                                                            gTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTg                                                                                      >  4:210396/1‑151 (MQ=255)
                                                            gTTGTAAGAAAAACAGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTg                                                                                      <  3:293668/151‑1 (MQ=255)
                                                                  aGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTg                                                                                <  4:1653263/151‑1 (MQ=255)
                                                                  aGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTg                                                                                >  1:251062/1‑151 (MQ=255)
                                                                   gAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTgg                                                                               >  3:423955/1‑151 (MQ=255)
                                                                     aaaaCGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGtt                                                                             >  3:311005/1‑151 (MQ=255)
                                                                     aaaaCGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGtt                                                                             <  4:510417/151‑1 (MQ=255)
                                                                         cGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGc                                                                         <  4:320717/151‑1 (MQ=255)
                                                                         cGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGc                                                                         >  4:1386526/1‑151 (MQ=255)
                                                                                tgCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCAcc                                                                  >  4:1683048/1‑151 (MQ=255)
                                                                                 gCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCg                                                                 >  1:258541/1‑151 (MQ=255)
                                                                                 gCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCg                                                                 <  4:449308/151‑1 (MQ=255)
                                                                                    gTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACt                                                              >  1:310649/1‑151 (MQ=255)
                                                                                    gTCTGGCATCAGGCCTCTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGAc                                                               <  2:745301/150‑1 (MQ=255)
                                                                                     tCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACtt                                                             >  4:1412324/1‑151 (MQ=255)
                                                                                     tCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACtt                                                             >  2:1359670/1‑151 (MQ=255)
                                                                                           aTCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACca                                                       <  2:619439/151‑1 (MQ=255)
                                                                                            tCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACcac                                                      >  2:350290/1‑151 (MQ=255)
                                                                                            tCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACcac                                                      >  4:794140/1‑151 (MQ=255)
                                                                                             cAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACcaca                                                     <  4:1624579/151‑1 (MQ=255)
                                                                                             cAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACcac                                                      >  4:145029/1‑150 (MQ=255)
                                                                                                gCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACg                                                  <  4:1139446/151‑1 (MQ=255)
                                                                                                 ccTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGc                                                 <  1:151892/151‑1 (MQ=255)
                                                                                                 ccTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGc                                                 <  2:1406039/151‑1 (MQ=255)
                                                                                                 ccTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGc                                                 <  2:950329/151‑1 (MQ=255)
                                                                                                    ttACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCgg                                              >  2:114319/1‑151 (MQ=255)
                                                                                                    ttACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCgg                                              >  2:539787/1‑151 (MQ=255)
                                                                                                     tACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCgg                                              <  4:143621/150‑1 (MQ=255)
                                                                                                        ccTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGcccc                                          >  2:316166/1‑151 (MQ=255)
                                                                                                          tGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCtt                                        >  4:625043/1‑151 (MQ=255)
                                                                                                             gTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCg                                      <  2:69855/150‑1 (MQ=255)
                                                                                                               ttCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTg                                    <  4:1204126/150‑1 (MQ=255)
                                                                                                                 cgcgAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGAcc                                 <  4:569784/151‑1 (MQ=255)
                                                                                                                   cgAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCg                                >  4:725915/1‑150 (MQ=255)
                                                                                                                    gAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCgag                              <  4:868183/151‑1 (MQ=255)
                                                                                                                    gAACCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCg                                >  2:1056952/1‑149 (MQ=255)
                                                                                                                     aaCCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCGTCGTGACCgaga                             <  2:662816/151‑1 (MQ=255)
                                                                                                                     aaCCGCAAAACAGAGGTTAGATTTGCCAGAGCGACAGAACTCACACGCGCCGCGCTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCTCAACGCCGGCCCCTTCGTGACCga                               <  2:1524421/149‑1 (MQ=255)
                                                                                                                      aCCGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGaa                            <  4:1504976/151‑1 (MQ=255)
                                                                                                                        cGCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAacc                          >  3:441792/1‑151 (MQ=255)
                                                                                                                        agCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAacc                          >  2:579646/2‑151 (MQ=255)
                                                                                                                         gCAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACGTGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAacc                          >  4:1132153/1‑150 (MQ=255)
                                                                                                                          cAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAaccac                        >  1:456641/1‑151 (MQ=255)
                                                                                                                          cAACACAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAacca                         <  2:1519897/150‑1 (MQ=255)
                                                                                                                            acacAGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCg                      <  1:423938/151‑1 (MQ=255)
                                                                                                                             cacaGAGGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCgg                     <  1:106920/151‑1 (MQ=255)
                                                                                                                                 gagGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCGGGAAt                 <  3:288606/151‑1 (MQ=255)
                                                                                                                                  agGTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCGGGAATa                <  1:321300/151‑1 (MQ=255)
                                                                                                                                    gTTAGTTTTGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCGGGAATac               >  4:1216441/1‑150 (MQ=255)
                                                                                                                                          tttCCCAGAGCGACAGAACTCACACTCGCCGCACTCCTCGGTGTAAACCGGGATCCCATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGCCTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCGGGAATACACCTTcc        >  1:386675/1‑151 (MQ=255)
                                                                                                                                           ttGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCGGGAATACACCTTcc        <  4:276017/150‑1 (MQ=255)
                                                                                                                                           gtGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCGGGAATACACCTTCCg       >  2:676502/2‑151 (MQ=255)
                                                                                                                                            tGCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCGGGAATACACCTTCCg       <  2:459889/150‑1 (MQ=255)
                                                                                                                                             gCCAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCGGGAATACACCTTCCgg      <  2:346846/150‑1 (MQ=255)
                                                                                                                                              ccAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCGGGAATACACCTTCCGGGt    >  3:389086/1‑151 (MQ=255)
                                                                                                                                               cAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCGGGAATACACCTTCCGGGTc   <  2:1503515/151‑1 (MQ=255)
                                                                                                                                               cAGAGCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCGGGAATACACCTTCCGGGTc   >  4:1039491/1‑151 (MQ=255)
                                                                                                                                                agagCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCGGGAATACACCTTCCGGGTCa  <  3:198398/151‑1 (MQ=255)
                                                                                                                                                agagCGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCGGGAATACACCTTCCGGGTCa  <  3:235705/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGCGACTACGGTGTATTCACTGAATGTAGAGCACCCCATGTAGTGATAAAGCGGCTGCCCGTTGTAAGAAAAACGGGTGGTGCCGTCTGGCATCAGGCCTTTACCCTGGGTTTCGCGAACCGCAACACAGAGGTTAGTTTTGCCAGAACGACAGAACTCACACTCGCCGCACTCCGCGGTGTAAAGCGGGATCACATGGTCGCCAGGTTTGACGCTGGTTACGCCTTCACCGACTTCAACCACAACGCCGGCCCCTTCGTGACCGAGAACCACCGGGAATACACCTTCCGGGTCA  >  NC_000913/379128‑379422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑