BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,636,906	C→T	A169T (GCG→ACG)	yhiN ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,636,906	0	C	T	99.5%	735.3 / NA	188	A169T (GCG→ACG)	yhiN	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (90/97); minor base A (1/0); total (91/97)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

CGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTT  >  NC_000913/3636759‑3637053

    aaccccGGAGGGCCCCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCa                                                                                                                                                <  2:1257743/144‑1 (MQ=255)

        aCGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCg                                                                                                                                          >  2:1534796/1‑151 (MQ=255)

          ggaggggcccGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGc                                                                                                                                        <  2:670182/143‑1 (MQ=255)

          ggAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGc                                                                                                                                        >  3:452233/1‑151 (MQ=255)

          gaagggCCCCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCg                                                                                                                                          <  2:1616917/145‑1 (MQ=255)

            agggCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCa                                                                                                                                       <  2:976768/148‑1 (MQ=255)

             aGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCAtt                                                                                                                                     >  4:643479/1‑151 (MQ=255)

              ggCCCCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCAt                                                                                                                                      <  2:346041/149‑1 (MQ=255)

              ggCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGc                                                                                                                                        <  1:270164/147‑1 (MQ=255)

                    gCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGcc                                                                                                                              >  3:375871/1‑151 (MQ=255)

                    gCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGcc                                                                                                                              >  4:1160269/1‑151 (MQ=255)

                     ccACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGccc                                                                                                                             >  2:520173/1‑151 (MQ=255)

                       aCGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCccac                                                                                                                           >  2:289557/1‑151 (MQ=255)

                        cgcccgccAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCccacc                                                                                                                          <  4:1546045/151‑1 (MQ=255)

                            cgccAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTc                                                                                                                      >  4:798122/1‑151 (MQ=255)

                                aGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTcgcg                                                                                                                   >  4:282592/1‑150 (MQ=255)

                                aGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTcgc                                                                                                                    >  2:1399881/1‑149 (MQ=255)

                                  cACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATg                                                                                                                >  4:710858/1‑151 (MQ=255)

                                  cACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATg                                                                                                                <  4:407226/151‑1 (MQ=255)

                                    ccTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGa                                                                                                               <  4:371983/150‑1 (MQ=255)

                                       gTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGAcc                                                                                                             <  4:62709/149‑1 (MQ=255)

                                       gTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGAcc                                                                                                             <  4:1648488/149‑1 (MQ=255)

                                        tAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGc                                                                                                          >  4:913251/1‑151 (MQ=255)

                                         aaCTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGc                                                                                                          >  2:360886/1‑150 (MQ=255)

                                         aaCTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCt                                                                                                         >  4:890494/1‑151 (MQ=255)

                                          aCTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCtt                                                                                                        <  4:43070/151‑1 (MQ=255)

                                             ctTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTc                                                                                                     >  2:922503/1‑151 (MQ=255)

                                              ttCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCg                                                                                                    >  2:1498861/1‑151 (MQ=255)

                                               tCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCtttt                                                                                                      <  1:84816/148‑1 (MQ=255)

                                               tCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCtttt                                                                                                      <  4:1479116/148‑1 (MQ=255)

                                               tCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCg                                                                                                    <  2:1572077/150‑1 (MQ=255)

                                                cGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCa                                                                                                  <  2:1123709/151‑1 (MQ=255)

                                                   gCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAAct                                                                                               <  2:108528/151‑2 (MQ=255)

                                                   gCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAAcc                                                                                               >  2:430025/1‑151 (MQ=255)

                                                     aaCGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCg                                                                                              >  4:534052/1‑150 (MQ=255)

                                                        ggTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGAc                                                                                            >  4:31550/1‑149 (MQ=255)

                                                        ggTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAg                                                                                          <  3:92100/151‑1 (MQ=255)

                                                        ggTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAg                                                                                          <  2:1178720/151‑1 (MQ=255)

                                                         gTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAg                                                                                          >  2:406529/1‑150 (MQ=255)

                                                            tATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCAt                                                                                      >  1:47055/1‑151 (MQ=255)

                                                              tGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGc                                                                                    >  4:1577000/1‑151 (MQ=255)

                                                               gCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGcc                                                                                   >  1:284317/1‑151 (MQ=255)

                                                                cAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCg                                                                                  <  4:1679222/151‑1 (MQ=255)

                                                                cAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCg                                                                                  >  2:1002407/1‑151 (MQ=255)

                                                                 atagTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGt                                                                                 >  4:210740/3‑151 (MQ=255)

                                                                    gTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGATCAGCTTTTCGCAACCGACAGTCATGCCGTTCa                                                                              <  2:862957/151‑1 (MQ=255)

                                                                    gTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTc                                                                               >  4:194509/1‑150 (MQ=255)

                                                                      gAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGa                                                                            <  3:175028/151‑1 (MQ=255)

                                                                       aaTGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTc                                                                               >  2:623606/1‑147 (MQ=255)

                                                                       aaTGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGAt                                                                           <  2:886473/151‑1 (MQ=255)

                                                                       aaTGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGAGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGt                                                                                 >  2:1024193/1‑145 (MQ=255)

                                                                        aTGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTc                                                                               >  4:1184289/1‑146 (MQ=255)

                                                                        aTGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATc                                                                          <  2:741461/151‑1 (MQ=255)

                                                                             aCCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCg                                                                     >  1:386753/1‑151 (MQ=255)

                                                                              ccAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCg                                                                     <  4:1359820/150‑1 (MQ=255)

                                                                                  aCCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGa                                                                  >  4:947198/1‑149 (MQ=255)

                                                                                    ccGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGcc                                                              >  2:26666/1‑151 (MQ=255)

                                                                                      gcgcgGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTg                                                            >  1:143833/1‑151 (MQ=255)

                                                                                      gcgcgGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTg                                                            >  4:1415167/1‑151 (MQ=255)

                                                                                        gcgGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGtt                                                          <  3:138575/151‑1 (MQ=255)

                                                                                         cgGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGttt                                                         <  3:227675/151‑1 (MQ=255)

                                                                                          gggCCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTc                                                        <  4:1683935/151‑1 (MQ=255)

                                                                                              cGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATcc                                                    <  1:452213/151‑1 (MQ=255)

                                                                                               ggCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCt                                                   <  3:352553/151‑1 (MQ=255)

                                                                                                gcagcaCGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCGCCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCtt                                                  <  4:1022071/151‑1 (MQ=255)

                                                                                                gcagcaCGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCtt                                                  <  4:1361484/151‑1 (MQ=255)

                                                                                                  agcaCGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCg                                                <  1:112040/151‑1 (MQ=255)

                                                                                                    caCGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGcc                                              >  4:208858/1‑151 (MQ=255)

                                                                                                     aCGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCgac                                              >  2:1201510/1‑148 (MQ=255)

                                                                                                         tGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCacac                                          <  2:1578573/150‑1 (MQ=255)

                                                                                                         tGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCacac                                          >  2:1459358/1‑150 (MQ=255)

                                                                                                              ccAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGc                                     >  2:208716/1‑150 (MQ=255)

                                                                                                              ccAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCa                                    <  2:1466214/151‑1 (MQ=255)

                                                                                                               cAAATTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCAc                                   >  2:1661483/1‑151 (MQ=255)

                                                                                                                aaaTTGTTCGGCAATCTTATACCCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAGCCGACGGTCATGCCGTGCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGc                                     <  1:397527/148‑1 (MQ=255)

                                                                                                                aaaTTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACt                                  >  1:446781/1‑151 (MQ=255)

                                                                                                                 aaTTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACtt                                 <  4:782116/151‑1 (MQ=255)

                                                                                                                  aTTGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTc                                <  2:482948/151‑1 (MQ=255)

                                                                                                                   ttGTTCGGCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCg                               >  4:1267785/1‑151 (MQ=255)

                                                                                                                          gCAATCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCa                        <  2:589761/151‑1 (MQ=255)

                                                                                                                            aaTCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAAt                      <  2:252196/151‑1 (MQ=255)

                                                                                                                            aaTCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAAt                      >  4:470523/1‑151 (MQ=255)

                                                                                                                              tCTTATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCt                    >  4:936959/1‑151 (MQ=255)

                                                                                                                                ttATAACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAACCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTg                   >  4:240329/1‑150 (MQ=255)

                                                                                                                                 tataACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAATCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGa                  <  2:1336578/150‑1 (MQ=255)

                                                                                                                                 tataACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCt                    <  4:750466/148‑1 (MQ=255)

                                                                                                                                 tataACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTg                   <  1:289155/149‑1 (MQ=255)

                                                                                                                                 tataACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGa                  <  4:346763/150‑1 (MQ=255)

                                                                                                                                  ataACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGaa                 <  2:970715/150‑1 (MQ=255)

                                                                                                                                  ataACCAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAg                <  4:1112108/151‑1 (MQ=255)

                                                                                                                                    aaCCAAACGGCGACGTGCCCAGCCTCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGt              <  2:192153/151‑1 (MQ=255)

                                                                                                                                      ccAAACGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTca            <  4:561480/151‑1 (MQ=255)

                                                                                                                                        aaaCGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTcaca          <  2:642609/151‑1 (MQ=255)

                                                                                                                                         aaCGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACAt         >  4:864116/1‑151 (MQ=255)

                                                                                                                                         aaCGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACAt         >  2:56912/1‑151 (MQ=255)

                                                                                                                                         aaCGGCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACAt         >  1:418345/1‑151 (MQ=255)

                                                                                                                                             gCGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGcc     >  4:1005537/1‑151 (MQ=255)

                                                                                                                                              cGACGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGccc    <  4:764369/151‑1 (MQ=255)

                                                                                                                                                aCGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGccc    <  2:807747/149‑1 (MQ=255)

                                                                                                                                                aCGTGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCtt  >  4:991245/1‑151 (MQ=255)

CGGTAATCACGGAAGGCACCGCCACGCCCGCCAGCACCTGTAACTCTTCGAGCAACGGTTTATGCAGAGTGAATGGCACCAGACCCGCGCGGGTCGGCAGCACGTTGAGGCCAAATTGTTCGGCAATCTTATAACCAAACGGCGACGCGCCCAGCCCCGGCATTGACAGCCCACCAGTCGCGATGACCAGCTTTTCGCAACCGACAGTCATGCCGTTCAGATCAAGCGTGAAGCCTGTTTCATCCTTCGCCACACTCAGCACTTCGCTACGCAATCTGAAGGTCACATTGCCCTT  >  NC_000913/3636759‑3637053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`