Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,655,517 A→G V129A (GTG→GCG)  yhiD ← putative Mg(2+) transport ATPase, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,655,5170AG99.5% 769.4 / ‑4.3 206V129A (GTG→GCG) yhiDputative Mg(2+) transport ATPase, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (112/93);  total (113/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

Only 100 of 206 total aligned reads displayed.

ATTTTCCTGCAACGAAACTAAATCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCG  >  NC_000913/3655369‑3655663
                                                                                                                                                    |                                                                                                                                                  
aTTTTCCTGCAACGAAACTAAATCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGcc                                                                                                                                                  >  2:674305/1‑151 (MQ=255)
aTTTTCCTGCAACGAAACTAAATCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGcc                                                                                                                                                  >  4:406339/1‑151 (MQ=255)
   ttCCTGCAACGAAACTAAATCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCcaac                                                                                                                                               >  3:110087/1‑151 (MQ=255)
     ccTGCAACGAAACTAAATCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCcaacaa                                                                                                                                             <  1:323171/151‑1 (MQ=255)
     ccTGCAACGAAACTAAATCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCcaaca                                                                                                                                              >  4:262226/1‑150 (MQ=255)
        gCAACGAAACTAAATCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGt                                                                                                                                          <  4:1060231/151‑1 (MQ=255)
        gCAACGAAACTAAATCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGt                                                                                                                                          <  4:494934/151‑1 (MQ=255)
          aaCGAAACTAAATCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGtca                                                                                                                                        <  4:1214218/151‑1 (MQ=255)
            cGAAACTAAATCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGtcatc                                                                                                                                      >  4:1550047/1‑151 (MQ=255)
               aaCTAAATCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGtcatca                                                                                                                                     <  4:431006/149‑1 (MQ=255)
                aCTAAATCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTg                                                                                                                                  <  1:375347/151‑1 (MQ=255)
                aCTAAATCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTg                                                                                                                                  <  2:370123/151‑1 (MQ=255)
                 cTAAATCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTg                                                                                                                                  >  2:188841/1‑150 (MQ=255)
                  tAAATCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGa                                                                                                                                 >  2:1076212/1‑150 (MQ=255)
                   aaaTCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGaa                                                                                                                                <  1:472014/150‑1 (MQ=255)
                    aaTCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAAcc                                                                                                                              >  4:374770/1‑151 (MQ=255)
                    aaTCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAAcc                                                                                                                              >  2:83203/1‑151 (MQ=255)
                       cAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCAt                                                                                                                            <  2:751444/150‑1 (MQ=255)
                       cAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATa                                                                                                                           <  2:1528140/151‑1 (MQ=255)
                               aTTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATAccc                                                                                                                    >  4:537295/1‑150 (MQ=255)
                                ttttttGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATAccc                                                                                                                    >  4:606664/1‑149 (MQ=255)
                                ttttttGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATAccc                                                                                                                    >  4:457082/1‑149 (MQ=255)
                                  ttttGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGt                                                                                                                 <  3:140482/150‑1 (MQ=255)
                                    ttGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCg                                                                                                              >  2:1506658/1‑151 (MQ=255)
                                    ttGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCg                                                                                                              >  2:710890/1‑151 (MQ=255)
                                      gctgctTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGt                                                                                                             >  4:1627663/1‑150 (MQ=255)
                                       ctgctTAAACCAGTCGCGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGtt                                                                                                                >  4:268919/1‑146 (MQ=255)
                                       ctgctTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGGCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATGCCCAGtt                                                                                                                <  2:268488/146‑1 (MQ=255)
                                        tgctTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTAc                                                                                                           >  2:63629/1‑150 (MQ=255)
                                          ctTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACAt                                                                                                         >  2:1312661/1‑150 (MQ=255)
                                          ctTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGATCGTACa                                                                                                          >  4:901860/1‑149 (MQ=255)
                                             aaaCCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCg                                                                                                     >  4:1446404/1‑151 (MQ=255)
                                             aaaCCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCg                                                                                                     <  4:1033710/151‑1 (MQ=255)
                                             aaaCCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCg                                                                                                     >  1:121496/1‑151 (MQ=255)
                                             aaaCCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCg                                                                                                     <  2:1438163/151‑1 (MQ=255)
                                              aaCCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGc                                                                                                    >  4:131167/1‑151 (MQ=255)
                                              aaCCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGc                                                                                                    >  4:1235069/1‑151 (MQ=255)
                                               aCCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCt                                                                                                   >  3:472002/1‑151 (MQ=255)
                                               aCCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCt                                                                                                   >  1:264469/1‑151 (MQ=255)
                                                ccAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTg                                                                                                  <  4:1389116/151‑1 (MQ=255)
                                                 cAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGc                                                                                                 <  2:456435/151‑1 (MQ=255)
                                                 cAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGc                                                                                                 >  4:347537/1‑151 (MQ=255)
                                                     cGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCaa                                                                                              <  3:42792/150‑1 (MQ=255)
                                                     cGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAAt                                                                                             >  2:774565/1‑151 (MQ=255)
                                                         cATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAAcc                                                                                         <  4:1660650/151‑1 (MQ=255)
                                                         cATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAAcc                                                                                         >  2:969197/1‑151 (MQ=255)
                                                          aTTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAAcc                                                                                         >  3:220662/1‑150 (MQ=255)
                                                          aTTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCa                                                                                        <  2:1623199/151‑1 (MQ=255)
                                                           ttGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCAt                                                                                       >  1:310815/1‑151 (MQ=255)
                                                                  cGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATg                                                                                <  2:39578/151‑1 (MQ=255)
                                                                   gTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATgg                                                                               >  2:572080/1‑151 (MQ=255)
                                                                     aTTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATggcg                                                                             <  2:55332/151‑1 (MQ=255)
                                                                     aTTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATggcg                                                                             >  4:1471158/1‑151 (MQ=255)
                                                                       tACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATggcggc                                                                           >  4:1413010/1‑151 (MQ=255)
                                                                       tACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATggcggc                                                                           >  4:737158/1‑151 (MQ=255)
                                                                       tACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATggcgg                                                                            <  4:1653619/150‑1 (MQ=255)
                                                                          cGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGt                                                                        >  2:436409/1‑151 (MQ=255)
                                                                           gTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTc                                                                       <  4:641228/151‑1 (MQ=255)
                                                                                 tAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCAccc                                                                   >  4:51213/1‑149 (MQ=255)
                                                                                   aTGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGat                                                               <  1:414579/151‑1 (MQ=255)
                                                                                    tGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGat                                                               >  1:142187/1‑150 (MQ=255)
                                                                                     gTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGatat                                                             <  4:189152/151‑1 (MQ=255)
                                                                                      tCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATc                                                            >  1:36289/1‑151 (MQ=255)
                                                                                       cAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATcc                                                           <  2:1401180/151‑1 (MQ=255)
                                                                                       cAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATc                                                            <  2:1594933/150‑1 (MQ=255)
                                                                                       cAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATc                                                            <  3:310832/150‑1 (MQ=255)
                                                                                          cTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCt                                                        >  4:572361/1‑151 (MQ=255)
                                                                                          cTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCt                                                        >  4:812774/1‑151 (MQ=255)
                                                                                           tGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTg                                                       >  3:94424/1‑151 (MQ=255)
                                                                                            gTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTg                                                       <  3:250523/150‑1 (MQ=255)
                                                                                               aaTGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCcgt                                                   >  1:199220/1‑151 (MQ=255)
                                                                                                  ggTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCcgtcg                                                 <  2:845720/150‑1 (MQ=255)
                                                                                                  ggTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCcgtcg                                                 <  2:907494/150‑1 (MQ=255)
                                                                                                  ggTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCcgtca                                                 <  2:1284755/150‑2 (MQ=255)
                                                                                                      aTTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCgtcgtc                                               >  2:1203270/1‑148 (MQ=255)
                                                                                                      aTTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGa                                            <  1:52558/151‑1 (MQ=255)
                                                                                                              tcatcaGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCTAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATACGCTGCCGTCATCTGCGCTACaa                                     >  2:475188/1‑150 (MQ=255)
                                                                                                              tcatcaGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACaa                                     <  2:1555291/150‑1 (MQ=255)
                                                                                                              tcatcaGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAat                                    >  3:452248/1‑151 (MQ=255)
                                                                                                               catcaGGCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAat                                    <  3:106138/150‑1 (MQ=255)
                                                                                                                     gCGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTgg                             <  2:1167106/151‑1 (MQ=255)
                                                                                                                      cGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGt                            >  2:106891/1‑151 (MQ=255)
                                                                                                                      cGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGt                            <  2:265231/151‑1 (MQ=255)
                                                                                                                      cGGAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGt                            <  4:1117799/151‑1 (MQ=255)
                                                                                                                       ggAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGtt                           >  3:357462/1‑151 (MQ=255)
                                                                                                                       ggAAGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGtt                           >  4:1177938/1‑151 (MQ=255)
                                                                                                                         aaGGTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTac                         <  4:93144/151‑1 (MQ=255)
                                                                                                                            gTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTacgaa                      <  4:956722/151‑1 (MQ=255)
                                                                                                                            gTTAATTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTacga                       >  2:310041/1‑150 (MQ=255)
                                                                                                                               aaTTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTacgaacg                    >  2:1651775/1‑150 (MQ=255)
                                                                                                                                aTTGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTacgaacg                    >  4:240780/1‑149 (MQ=255)
                                                                                                                                  tGATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACgaga                 <  4:661790/150‑1 (MQ=255)
                                                                                                                                   gATGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAAt               <  4:1508542/151‑1 (MQ=255)
                                                                                                                                    aTGGAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATg              >  3:414330/1‑151 (MQ=255)
                                                                                                                                      ggAAGACTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGt             <  4:873028/150‑1 (MQ=255)
                                                                                                                                           aCTTCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTg       <  2:805331/151‑1 (MQ=255)
                                                                                                                                             ttCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGc      >  3:381554/1‑150 (MQ=255)
                                                                                                                                              tCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCa     <  2:405753/150‑1 (MQ=255)
                                                                                                                                              tCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCa     <  4:1602247/150‑1 (MQ=255)
                                                                                                                                              tCCAGCGCCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCa     <  3:23280/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATTTTCCTGCAACGAAACTAAATCAGTTTTTATTTTTTGCTGCTTAAACCAGTCGAGCATTGATACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCG  >  NC_000913/3655369‑3655663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: