BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	396,507	A→G	intergenic (‑220/‑132)	ampH ← / → sbmA	D‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase; penicillin‑binding protein; weak beta‑lactamase/peptide antibiotic transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	396,507	0	A	G	99.1%	445.7 / ‑4.5	114	intergenic (‑220/‑132)	ampH/sbmA	D‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase; penicillin‑binding protein; weak beta‑lactamase/peptide antibiotic transporter
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (55/58); total (56/58)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 114 total aligned reads displayed.

AATTAGTGCAATAATAGTGTTAAAAACGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTT  >  NC_000913/396360‑396653

aaTTAGTGCAATAATAGTGTTAAAAACGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTgg                                                                                                                                                 <  3:39980/151‑1 (MQ=255)

aaTTAGTGCAATAATAGTGTTAAAAACGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTgg                                                                                                                                                 >  2:881330/1‑151 (MQ=255)

    aGTGCAATAATAGTGTTAAAAACGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTc                                                                                                                                               >  4:689982/1‑149 (MQ=255)

     gTGCAATAATAGTGTTAAAAACGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAgg                                                                                                                                            >  2:476955/1‑151 (MQ=255)

       gCAATAATAGTGTTAAAAACGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCt                                                                                                                                          <  4:1666704/151‑1 (MQ=255)

          ataataGTGTTAAAAACGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGc                                                                                                                                       >  2:1341438/1‑151 (MQ=255)

            aataGTGTTAAAAACGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCg                                                                                                                                      >  2:99339/1‑150 (MQ=255)

            aataGTGTTAAAAACGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGa                                                                                                                                     <  2:1649679/151‑1 (MQ=255)

             ataGTGTTAAAAACGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGaa                                                                                                                                    <  4:1358292/151‑1 (MQ=255)

              taGTGTTAAAAACGTAACGGCGCGTACACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAAc                                                                                                                                   >  1:72406/1‑151 (MQ=255)

               aGTGTTAAAAACGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACt                                                                                                                                  <  4:1500113/151‑1 (MQ=255)

               aGTGTTAAAAACGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACt                                                                                                                                  <  4:1305130/151‑1 (MQ=255)

               aGTGTTAAAAACGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACt                                                                                                                                  >  4:898317/1‑151 (MQ=255)

                 tgtTAAAAACGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTa                                                                                                                                 >  4:613217/1‑150 (MQ=255)

                  gtTAAAAACGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTa                                                                                                                                 >  1:4866/1‑149 (MQ=255)

                  gtTAAAAACGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAg                                                                                                                               <  4:1335720/151‑1 (MQ=255)

                  gtTAAAAACGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAg                                                                                                                               <  2:698846/151‑1 (MQ=255)

                       aaaCGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATACGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGcc                                                                                                                           >  1:220501/1‑150 (MQ=255)

                        aaCGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCtt                                                                                                                         >  4:644976/1‑151 (MQ=255)

                        aaCGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCtt                                                                                                                         <  4:503982/151‑1 (MQ=255)

                         aCGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTg                                                                                                                        >  2:319155/1‑151 (MQ=255)

                         aCGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTg                                                                                                                        >  4:1535985/1‑151 (MQ=255)

                              aCGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATg                                                                                                                   <  4:1365927/151‑1 (MQ=255)

                                ggCGCGTAAACACGATCTTTTCCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATggg                                                                                                                 >  4:917266/1‑151 (MQ=255)

                                  cgcgTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTc                                                                                                               >  2:223371/1‑151 (MQ=255)

                                   gcgTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTca                                                                                                              <  2:1478025/151‑1 (MQ=255)

                                    cgTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTcac                                                                                                             <  1:206745/151‑1 (MQ=255)

                                     gTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTcaca                                                                                                            <  4:665959/151‑1 (MQ=255)

                                     gTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTcac                                                                                                             >  2:567266/1‑150 (MQ=255)

                                      tAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTcaca                                                                                                            >  4:442940/1‑150 (MQ=255)

                                       aaaCACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAAt                                                                                                          <  4:1050779/151‑1 (MQ=255)

                                           acGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGc                                                                                                      <  4:1443399/151‑1 (MQ=255)

                                           acGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGc                                                                                                      >  4:1150411/1‑151 (MQ=255)

                                            cGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCg                                                                                                     >  3:24609/1‑151 (MQ=255)

                                                cTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGttt                                                                                                 >  4:884388/1‑151 (MQ=255)

                                                 tttttCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAg                                                                                                                                             <  1:428102/106‑1 (MQ=255)

                                                  ttttCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGtt                                                                                                  <  4:152049/148‑1 (MQ=255)

                                                    ttCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCAt                                                                                              <  4:568046/150‑1 (MQ=255)

                                                      ccTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCAttt                                                                                            <  2:1285230/150‑1 (MQ=255)

                                                      ccTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTg                                                                                           >  1:477143/1‑151 (MQ=255)

                                                        tGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGc                                                                                         >  1:299451/1‑151 (MQ=255)

                                                         gCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCt                                                                                        <  4:26359/151‑1 (MQ=255)

                                                         gCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCt                                                                                        <  1:200588/151‑1 (MQ=255)

                                                           tCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTAGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTg                                                                                       >  4:649231/1‑150 (MQ=255)

                                                             aCCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACg                                                                                    >  2:283583/1‑151 (MQ=255)

                                                                 aTGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCttt                                                                                <  1:467683/151‑1 (MQ=255)

                                                                   gCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGt                                                                              >  2:1526392/1‑151 (MQ=255)

                                                                       gCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGc                                                                           <  2:1208313/150‑1 (MQ=255)

                                                                         gtttGGGATCCTGCGCGTGTTAGTAAGCTTATGTAGACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTATGGTCAAGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCgg                                                                         >  2:99607/2‑150 (MQ=255)

                                                                         aTTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGt                                                                        >  3:209329/1‑151 (MQ=255)

                                                                          tttGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTc                                                                       >  1:316469/1‑151 (MQ=255)

                                                                           ttGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTc                                                                       <  4:1266075/150‑1 (MQ=255)

                                                                            tgtgATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCa                                                                      <  4:345441/150‑1 (MQ=255)

                                                                            tgtgATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCa                                                                      <  1:394174/150‑1 (MQ=255)

                                                                             gtgATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCAt                                                                     <  4:1531289/150‑1 (MQ=255)

                                                                               gATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCg                                                                  >  4:1645287/1‑151 (MQ=255)

                                                                               gATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCg                                                                  <  4:803977/151‑1 (MQ=255)

                                                                                aTCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCgg                                                                 >  2:852834/1‑151 (MQ=255)

                                                                                  ccTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGtt                                                               <  4:284029/151‑1 (MQ=255)

                                                                                  ccTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGtt                                                               <  3:4866/151‑1 (MQ=255)

                                                                                    tGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTa                                                              >  2:313307/1‑150 (MQ=255)

                                                                                     gcgcGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAAt                                                            <  2:442313/151‑1 (MQ=255)

                                                                                      cgcgTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATa                                                           <  4:27180/151‑1 (MQ=255)

                                                                                        cgTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATac                                                          >  2:594441/1‑150 (MQ=255)

                                                                                          tgtTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATacac                                                        >  2:156716/1‑150 (MQ=255)

                                                                                           gtTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATAcaca                                                       <  1:54066/150‑1 (MQ=255)

                                                                                           gtTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATAcaca                                                       <  4:1047042/150‑1 (MQ=255)

                                                                                           gtTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACAcag                                                      <  4:70995/151‑1 (MQ=255)

                                                                                             tAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACAcagc                                                     <  4:205126/150‑1 (MQ=255)

                                                                                              aaTCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACAcagc                                                     >  4:1046314/1‑149 (MQ=255)

                                                                                                 ccGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAgtt                                                 <  3:88082/150‑1 (MQ=255)

                                                                                                 ccGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAgtt                                                 <  1:69287/150‑1 (MQ=255)

                                                                                                   gCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAgttgtt                                              >  4:529077/1‑151 (MQ=255)

                                                                                                     ttATGTAAACAATATTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTa                                             >  3:77069/1‑150 (MQ=255)

                                                                                                      tATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTa                                             <  4:1476733/149‑1 (MQ=255)

                                                                                                       aTGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTaaaa                                          <  3:299468/151‑1 (MQ=255)

                                                                                                       aTGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTaaa                                           >  2:1006977/1‑150 (MQ=255)

                                                                                                              cAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATa                                     >  2:961981/1‑149 (MQ=255)

                                                                                                               aaTCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATaaga                                  <  4:1122827/151‑1 (MQ=255)

                                                                                                                aTCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATaaga                                  >  2:1544909/1‑150 (MQ=255)

                                                                                                                    ttGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATaagaag                                <  3:316486/148‑1 (MQ=255)

                                                                                                                    ttGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGtt                              >  3:65598/1‑150 (MQ=255)

                                                                                                                    ttGGTTTGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGt                               <  1:128896/149‑1 (MQ=255)

                                                                                                                        tttGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGc                           >  4:752218/1‑149 (MQ=255)

                                                                                                                         ttGTGAAATCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAgg                        >  4:154904/1‑151 (MQ=255)

                                                                                                                                aTCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCat                 <  3:195580/151‑1 (MQ=255)

                                                                                                                                 tCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCat                 >  2:1527903/1‑150 (MQ=255)

                                                                                                                                 tCCGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCat                 >  3:472370/1‑150 (MQ=255)

                                                                                                                                  ccGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCatat               >  4:321074/1‑151 (MQ=255)

                                                                                                                                  ccGTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCata                <  2:689045/150‑1 (MQ=255)

                                                                                                                                    gTCGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGt             <  4:1505094/151‑1 (MQ=255)

                                                                                                                                      cGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCGGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGttt           >  2:472438/1‑151 (MQ=255)

                                                                                                                                      cGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGttt           >  4:110222/1‑151 (MQ=255)

                                                                                                                                      cGAAACAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGttt           <  4:477627/151‑1 (MQ=255)

                                                                                                                                         aaCAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAg        <  2:1534078/151‑1 (MQ=255)

                                                                                                                                           cAATTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTc      <  4:1565305/151‑1 (MQ=255)

                                                                                                                                            aaTTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCt     >  2:51935/1‑151 (MQ=255)

                                                                                                                                             aTTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCtt    >  4:1143690/1‑151 (MQ=255)

                                                                                                                                             aTTCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCtt    <  2:875065/151‑1 (MQ=255)

                                                                                                                                               tCTTGTGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCtttt  >  2:903312/1‑151 (MQ=255)

AATTAGTGCAATAATAGTGTTAAAAACGTAACGGCGCGTAAACACGATCTTTTTCCTGCTCACCAATGCCTGCATTTGTGATCCTGCGCGTGTTAATCCGCTTATGTAAACAATCTTTGGTTTGTGAAATCCGTCGAAACAATTCTTATGGTCAGGCTGGCGAACTAAGCGCCTTGCTATGGGTCACAATGGGCGGTTTCATTTGGCTGACGCTTTGTAGCGGTCATGCGGTTAATACACAGCAGTTGTTAAAACGATAAGAAGTTAGCAGGAGTGCATATGTTTAAGTCTTTT  >  NC_000913/396360‑396653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`