Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,832,490 T→C H683R (CAC→CGC)  yicI ← putative alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,832,4900TC97.2% 365.0 / ‑4.7 108H683R (CAC→CGC) yicIputative alpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base C (50/54);  total (50/58)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 110 total aligned reads displayed.

CTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGTGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCCACAGGTGTGTCCAGC  >  NC_000913/3832342‑3832637
                                                                                                                                                    |                                                                                                                                                   
cttcGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCgcgc                                                                                                                                                   >  4:905971/1‑151 (MQ=255)
cttcGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCgcgc                                                                                                                                                   >  4:306817/1‑151 (MQ=255)
 ttcGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGcc                                                                                                                                                  >  2:908411/1‑151 (MQ=255)
     cctcgccCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCacac                                                                                                                                              <  4:1211327/151‑1 (MQ=255)
         gccCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTaa                                                                                                                                          <  2:1629909/151‑1 (MQ=255)
         gccCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTa                                                                                                                                           >  1:166790/1‑150 (MQ=255)
         gccCGCACAAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTa                                                                                                                                           >  2:1218636/1‑150 (MQ=255)
            cGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCg                                                                                                                                       <  3:232080/151‑1 (MQ=255)
            cGCACCAGTAACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGAAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGCAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCg                                                                                                                                       >  2:1503226/1‑151 (MQ=255)
             gCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCgg                                                                                                                                      >  4:1561791/1‑151 (MQ=255)
             gCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCgg                                                                                                                                      <  3:166804/151‑1 (MQ=255)
                     cacaGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTg                                                                                                                               >  4:126136/1‑150 (MQ=255)
                     cacaGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGa                                                                                                                              <  4:662559/151‑1 (MQ=255)
                      acaGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGAt                                                                                                                             >  4:833674/1‑151 (MQ=255)
                       caGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGAt                                                                                                                             >  2:607707/1‑150 (MQ=255)
                       caGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATc                                                                                                                            <  4:737764/151‑1 (MQ=255)
                         gTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCgt                                                                                                                          <  4:400976/151‑1 (MQ=255)
                         gTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCgt                                                                                                                          <  2:556314/151‑1 (MQ=255)
                            aTCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCgttgt                                                                                                                       <  2:1150771/151‑1 (MQ=255)
                              cGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGttgttg                                                                                                                     >  2:284661/1‑151 (MQ=255)
                               gtgtTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGc                                                                                                                    <  4:229005/151‑1 (MQ=255)
                                 gtTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGccc                                                                                                                  <  4:68398/151‑1 (MQ=255)
                                   tGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGccc                                                                                                                  >  4:297569/1‑149 (MQ=255)
                                    gCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAgc                                                                                                               <  2:712420/151‑1 (MQ=255)
                                      cAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAgcgc                                                                                                             >  4:1522595/1‑151 (MQ=255)
                                      cAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAgcg                                                                                                              <  3:360037/150‑1 (MQ=255)
                                       aGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGcc                                                                                                            >  4:375156/1‑151 (MQ=255)
                                       aGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCGTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGcc                                                                                                            <  2:962418/151‑1 (MQ=255)
                                        gTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGcc                                                                                                            >  2:1629268/1‑150 (MQ=255)
                                          aCGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGt                                                                                                         <  2:304467/151‑1 (MQ=255)
                                           cGTTCTGATTTGAAAGTAAACATCATCGTTTCTTCAGCAGCCGTCCCTTCTCAGACGGGTGCATGCCCGTCTTGCAGATTGAAGAGGCGTCATGCTTTCCCTGCGTGCTACACGTAACCTCTTCGTTGATCGTTGTTGCGCTGCGCCTGTa                                                                                                        <  2:1165264/151‑1 (MQ=255)
                                           cGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGt                                                                                                         >  3:305835/1‑150 (MQ=255)
                                            gTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTag                                                                                                       <  2:350863/151‑1 (MQ=255)
                                              gctgctTTTAAAGTAAAGATCACCGTTCCTTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGTAATGCCGTGCCTTCGCGCCACTCGTAATCGGTACGTTGATCGTTGTTGCCCAGCGCCAGTaga                                                                                                      <  2:418687/150‑1 (MQ=255)
                                              gctgctTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTaga                                                                                                      <  2:1118871/150‑1 (MQ=255)
                                               ctgctTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAgt                                                                                                    >  2:665389/1‑151 (MQ=255)
                                                     ttAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATc                                                                                              >  4:1251738/1‑151 (MQ=255)
                                                      taaagtaaagATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATc                                                                                              <  2:479237/150‑1 (MQ=255)
                                                       aaagtaaagATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCAc                                                                                            <  3:370741/151‑1 (MQ=255)
                                                         agtaaagATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGc                                                                                          >  2:826526/1‑151 (MQ=255)
                                                          gtaaagATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGc                                                                                          >  2:60383/1‑150 (MQ=255)
                                                             aagATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACAt                                                                                      >  2:822548/1‑151 (MQ=255)
                                                               gATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATaa                                                                                    <  4:875652/151‑1 (MQ=255)
                                                               gATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGAGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATaa                                                                                    <  4:1567465/151‑1 (MQ=255)
                                                                aTCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATaa                                                                                    >  4:1072439/1‑150 (MQ=255)
                                                                 tCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAAc                                                                                  >  3:253406/1‑151 (MQ=255)
                                                                     cGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGc                                                                              >  4:305045/1‑151 (MQ=255)
                                                                       aTCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACgg                                                                                >  4:57028/1‑147 (MQ=255)
                                                                       aTCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGc                                                                              >  4:1063/1‑149 (MQ=255)
                                                                         ccGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGa                                                                           <  2:1576486/150‑1 (MQ=255)
                                                                         ccGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGAc                                                                          >  1:306173/1‑151 (MQ=255)
                                                                          cGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACt                                                                         >  4:1600978/1‑151 (MQ=255)
                                                                           gTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTc                                                                        <  2:1172262/151‑1 (MQ=255)
                                                                             cagcagCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCa                                                                       <  1:109818/150‑1 (MQ=255)
                                                                              agcagcGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAgg                                                                     >  1:350291/1‑151 (MQ=255)
                                                                              agcagcGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAgg                                                                     <  4:829834/151‑1 (MQ=255)
                                                                               gcagcGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAgg                                                                     >  3:36490/1‑150 (MQ=255)
                                                                               gcagcGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGa                                                                    <  2:75189/151‑1 (MQ=255)
                                                                                   cGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGcc                                                                <  4:742832/151‑1 (MQ=255)
                                                                                   cGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGcc                                                                <  2:123441/151‑1 (MQ=255)
                                                                                   cGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGcc                                                                <  4:1035053/151‑1 (MQ=255)
                                                                                     ggCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGt                                                              <  4:1300394/151‑1 (MQ=255)
                                                                                        aCTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGtgct                                                           >  2:1435481/1‑151 (MQ=255)
                                                                                          ttCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGtgctgc                                                         >  2:978436/1‑151 (MQ=255)
                                                                                          ttCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGtgctgc                                                         >  4:1277222/1‑151 (MQ=255)
                                                                                          ttCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGtgctgc                                                         >  3:449279/1‑151 (MQ=255)
                                                                                            cacaGACGGCTTCATGCCCCTCTTGGAGATTGAAGAGGTGGAATGCCGCGCCCTCGTGCCGCACGTAATCGGGACGTTGATCGTTGTTGACCAGCGCCAGTAGAGTATTATCACGCCCATAATCGGGCCGACTCAGGAAGACGCGctgctg                                                       <  2:1472692/151‑1 (MQ=255)
                                                                                              caGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGtt                                                     <  4:1431469/151‑1 (MQ=255)
                                                                                              caGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGtt                                                     >  1:384693/1‑151 (MQ=255)
                                                                                               aGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGttt                                                    >  2:1292374/1‑151 (MQ=255)
                                                                                               aGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGttt                                                    >  1:135926/1‑151 (MQ=255)
                                                                                                 aCGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTAt                                                  <  2:1456622/151‑1 (MQ=255)
                                                                                                  cGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATg                                                 >  4:1190416/1‑151 (MQ=255)
                                                                                                  cGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATg                                                 <  2:822048/151‑1 (MQ=255)
                                                                                                  cGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATg                                                 >  3:179403/1‑151 (MQ=255)
                                                                                                         aTGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCg                                           >  2:473095/1‑150 (MQ=255)
                                                                                                         aTGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGa                                          <  4:258851/151‑1 (MQ=255)
                                                                                                           gCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACt                                        <  3:406888/151‑1 (MQ=255)
                                                                                                               gTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTAc                                      <  1:166575/149‑1 (MQ=255)
                                                                                                                tCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGt                                   >  2:154778/1‑151 (MQ=255)
                                                                                                                      aGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGtt                             <  2:780876/151‑1 (MQ=255)
                                                                                                                      aGATTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGtt                             <  4:285107/151‑1 (MQ=255)
                                                                                                                        aTTGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCa                           <  2:1240928/151‑1 (MQ=255)
                                                                                                                          tGAAGAGGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCAt                          <  2:444748/150‑1 (MQ=255)
                                                                                                                            aaGAGGTGGAATGCCGTGCCTTCGTTCCACATGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGACGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCg                        <  2:783367/150‑1 (MQ=255)
                                                                                                                             agagGTGGAATGCCGTGCCTTCGCGCCACACGTATTCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCCGGAAGCCGTGCTGCTGTTTATGCAAGCGACTGCCGTCGAGTTCATCGtt                      <  2:1319977/151‑1 (MQ=255)
                                                                                                                               agGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTtgt                    <  1:42133/151‑1 (MQ=255)
                                                                                                                               agGTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTtgt                    >  2:1642923/1‑151 (MQ=255)
                                                                                                                                ggTGGAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTtgtg                   <  4:101758/151‑1 (MQ=255)
                                                                                                                                    gAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCcac               >  2:574192/1‑151 (MQ=255)
                                                                                                                                    gAATGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCcac               >  4:1588333/1‑151 (MQ=255)
                                                                                                                                     aaTGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCcac               >  2:500969/1‑150 (MQ=255)
                                                                                                                                     aaTGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCcac               >  4:623675/1‑150 (MQ=255)
                                                                                                                                     aaTGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCcac               >  1:477763/1‑150 (MQ=255)
                                                                                                                                      aTGCCGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCCACAg             <  2:1250162/151‑1 (MQ=255)
                                                                                                                                         ccGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCCACAGgtg          <  2:1452451/151‑1 (MQ=255)
                                                                                                                                          cGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCCACAGgtgt         <  3:64430/151‑1 (MQ=255)
                                                                                                                                          cGTGCCTTCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCCACAGgtgt         >  4:295249/1‑151 (MQ=255)
                                                                                                                                                 tCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCCACAGGTGTGTCCa    <  4:262542/149‑1 (MQ=255)
                                                                                                                                                 tCGCGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCCACAGGTGTGTCCAGc  >  1:462745/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTTCGCCTCGCCCGCACCAGTCACAGTAATCGTGTTGCCAGTACGTGCTGCTTTTAAAGTAAAGATCACCGATCCGTCAGCAGCGGGCACTTCACAGACGGCTTCATGCCCGTCTTGCAGATTGAAGAGGTGGAATGCCGTGCCTTCGTGCCACACGTAATCGGGACGTTGATCGTTGTTGCCCAGCGCCAGTAGAGTGTTATCACGCACATAAACGGGCAGACTCAGGAAGCCGTGCTGCTGTTTATGCCAGCGACTACCGTCGAGTTCATCGTTGTGCCACAGGTGTGTCCAGC  >  NC_000913/3832342‑3832637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: