BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,932,390	C→T	H359Y (CAC→TAC)	kup →	potassium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,932,390	0	C	T	99.2%	493.6 / ‑4.8	128	H359Y (CAC→TAC)	kup	potassium transporter
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (64/62); total (65/63)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 128 total aligned reads displayed.

CTCATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCG  >  NC_000913/3932242‑3932537

ctcATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTAc                                                                                                                                                   >  3:173573/1‑151 (MQ=255)

         gcgTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAAc                                                                                                                                          <  1:272428/151‑1 (MQ=255)

          cgTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAAcc                                                                                                                                         >  4:1454699/1‑151 (MQ=255)

           gTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCt                                                                                                                                        <  2:641081/151‑1 (MQ=255)

           gTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCt                                                                                                                                        >  2:299694/1‑151 (MQ=255)

            tCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGACGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTg                                                                                                                                       >  2:349756/1‑151 (MQ=255)

             cAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTgg                                                                                                                                      >  4:345997/1‑151 (MQ=255)

             cAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTg                                                                                                                                       <  2:1605717/150‑1 (MQ=255)

                gCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTggcgg                                                                                                                                   <  2:892730/151‑1 (MQ=255)

                 cGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTggcggc                                                                                                                                  <  2:1116710/151‑1 (MQ=255)

                   gTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTggcggcgg                                                                                                                                >  2:57105/1‑151 (MQ=255)

                     aCGTCTGGGATATTTGTCGCCGATTCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGgcggcggcg                                                                                                                              >  4:778648/1‑151 (MQ=255)

                     aCGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGgcggcggcg                                                                                                                              >  4:1115405/1‑151 (MQ=255)

                          tGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACgg                                                                                                                         >  2:930845/1‑151 (MQ=255)

                                    gTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGt                                                                                                                <  4:710565/150‑1 (MQ=255)

                                      cGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGAc                                                                                                             <  3:7877/151‑1 (MQ=255)

                                       gCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGAcc                                                                                                            <  2:479769/151‑1 (MQ=255)

                                         cGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCgg                                                                                                          <  1:454944/151‑1 (MQ=255)

                                         cGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCgg                                                                                                          >  2:269554/1‑151 (MQ=255)

                                         cGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCgg                                                                                                          >  4:670286/1‑151 (MQ=255)

                                          gATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGa                                                                                                         <  4:1012658/151‑1 (MQ=255)

                                          gATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGa                                                                                                         <  2:45570/151‑1 (MQ=255)

                                           aTGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGa                                                                                                         >  1:106894/1‑150 (MQ=255)

                                           aTGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGa                                                                                                         >  2:277243/1‑150 (MQ=255)

                                             gcgcATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAAcc                                                                                                      >  2:339382/1‑151 (MQ=255)

                                             gcgcATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAAcc                                                                                                      <  4:1495633/151‑1 (MQ=255)

                                             gcgcATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAAc                                                                                                       <  4:727956/150‑1 (MQ=255)

                                              cgcATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCa                                                                                                     <  4:365464/151‑1 (MQ=255)

                                               gcATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCAt                                                                                                    <  2:1117459/151‑1 (MQ=255)

                                                cATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATg                                                                                                   <  2:558557/151‑1 (MQ=255)

                                                 attattCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATgg                                                                                                  <  3:458911/151‑1 (MQ=255)

                                                 attattCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATgg                                                                                                  <  4:122529/151‑1 (MQ=255)

                                                 attattCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATg                                                                                                   <  4:1680464/150‑1 (MQ=255)

                                                       cacaCCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTg                                                                                             <  3:65405/150‑1 (MQ=255)

                                                        acacCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGAc                                                                                           <  2:834773/151‑1 (MQ=255)

                                                          acCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGt                                                                                         <  4:1112267/151‑1 (MQ=255)

                                                            cTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCt                                                                                       >  1:434148/1‑151 (MQ=255)

                                                             tCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCt                                                                                       >  1:117978/1‑150 (MQ=255)

                                                              ccGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTAt                                                                                     >  4:942540/1‑151 (MQ=255)

                                                              ccGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTAt                                                                                     <  2:1202892/151‑1 (MQ=255)

                                                               cGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTAtt                                                                                    <  2:995898/151‑1 (MQ=255)

                                                                gAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATtc                                                                                   >  1:253321/1‑151 (MQ=255)

                                                                 aaaTGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATtct                                                                                  <  4:1135742/151‑1 (MQ=255)

                                                                 aaaTGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATtct                                                                                  >  4:70705/1‑151 (MQ=255)

                                                                      gAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGa                                                                             <  2:482343/151‑1 (MQ=255)

                                                                           aGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGAGGCGTCCGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTAcc                                                                        >  1:216503/1‑151 (MQ=255)

                                                                                aaaTCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGc                                                                   >  2:1321921/1‑151 (MQ=255)

                                                                                 aaTCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGc                                                                   >  1:409579/1‑150 (MQ=255)

                                                                                 aaTCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGc                                                                   >  2:613271/1‑150 (MQ=255)

                                                                                 aaTCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGc                                                                   >  1:466676/1‑150 (MQ=255)

                                                                                 aaTCGATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGGACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCa                                                                  <  1:387725/151‑1 (MQ=255)

                                                                                   tCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACg                                                                >  2:1574785/1‑151 (MQ=255)

                                                                                     tataTTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTc                                                              >  4:1444722/1‑151 (MQ=255)

                                                                                      atatTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCa                                                             <  2:1513669/151‑1 (MQ=255)

                                                                                       tatTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTc                                                              >  2:64646/1‑149 (MQ=255)

                                                                                        atTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACg                                                                >  2:1293677/1‑146 (MQ=255)

                                                                                        atTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTc                                                              >  4:256933/1‑148 (MQ=255)

                                                                                          tCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGGACTCGATCAACGTGGCGGCGGCGTACGGGATTGCGGTGACCGGCACCATGGAGCTGACGTCTATTCTATCGACTACCGGGGCACGTCAGAAc                                                         >  4:210172/1‑151 (MQ=255)

                                                                                                tgtgAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCa                                                    <  4:300161/150‑1 (MQ=255)

                                                                                                tgtgAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCAc                                                   >  3:380028/1‑151 (MQ=255)

                                                                                                tgtgAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCAc                                                   >  1:208449/1‑151 (MQ=255)

                                                                                                 gtgAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACt                                                  >  4:1389089/1‑151 (MQ=255)

                                                                                                 gtgAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACt                                                  >  2:154884/1‑151 (MQ=255)

                                                                                                 gtgAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACt                                                  <  2:1605928/151‑1 (MQ=255)

                                                                                                       tGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAAt                                             >  4:193320/1‑150 (MQ=255)

                                                                                                       tGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAAt                                             >  1:129075/1‑150 (MQ=255)

                                                                                                        ggATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAAt                                             <  2:345480/149‑1 (MQ=255)

                                                                                                        ggATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATaa                                           <  1:33091/151‑1 (MQ=255)

                                                                                                        ggATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATaa                                           <  3:338050/151‑1 (MQ=255)

                                                                                                            gCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTa                                        <  2:1227999/150‑1 (MQ=255)

                                                                                                            gCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTAt                                       <  1:158003/151‑1 (MQ=255)

                                                                                                            gCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGAAt                                       >  4:125199/1‑151 (MQ=255)

                                                                                                                 aTGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTAtttt                                    <  2:546756/149‑1 (MQ=255)

                                                                                                                 aTGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTttg                                   <  2:1401324/150‑1 (MQ=255)

                                                                                                                 aTGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTttg                                   <  4:1457371/150‑1 (MQ=255)

                                                                                                                     cgcgGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTgcg                              <  2:674300/151‑1 (MQ=255)

                                                                                                                      gcgGTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTgcgc                             >  4:1222533/1‑151 (MQ=255)

                                                                                                                        ggTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTg                           <  1:235744/151‑1 (MQ=255)

                                                                                                                        ggTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTg                           >  3:246511/1‑151 (MQ=255)

                                                                                                                         gTCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGa                          <  1:179899/151‑1 (MQ=255)

                                                                                                                          tCGTGATTGTGATTGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGAt                         >  2:1032155/1‑151 (MQ=255)

                                                                                                                            gtgattgtgattGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATcc                       >  2:1142898/1‑151 (MQ=255)

                                                                                                                            gtgattgtgattGTCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGAGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATcc                       >  2:190372/1‑151 (MQ=255)

                                                                                                                              gattgtgattgtCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCt                      <  4:584463/150‑1 (MQ=255)

                                                                                                                              gattgtgattgtCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTg                     <  4:22310/151‑1 (MQ=255)

                                                                                                                              gattgtgattgtCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTg                     >  4:1082866/1‑151 (MQ=255)

                                                                                                                              gattgtgattgtCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTg                     <  4:729559/151‑1 (MQ=255)

                                                                                                                               attgtgattgtCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTg                     >  2:1495577/1‑150 (MQ=255)

                                                                                                                                  gtgattgtCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGGCTACCGTTGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGAtt                  <  3:216518/150‑1 (MQ=255)

                                                                                                                                  gtgattgtCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGAtt                  <  2:1433426/150‑1 (MQ=255)

                                                                                                                                  gtgattgtCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTg                 <  4:339891/151‑1 (MQ=255)

                                                                                                                                   tgattgtCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGc                >  2:1466510/1‑151 (MQ=255)

                                                                                                                                    gattgtCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCt               >  4:296882/1‑151 (MQ=255)

                                                                                                                                        gtCAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTcc           <  4:235974/151‑1 (MQ=255)

                                                                                                                                          cAGCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCtt         <  1:83733/151‑1 (MQ=255)

                                                                                                                                            gCTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCGGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCttt        >  2:1241581/1‑150 (MQ=255)

                                                                                                                                             cTTTGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTtgt      >  2:265303/1‑151 (MQ=255)

                                                                                                                                                tGAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTgtgt    <  2:591441/150‑1 (MQ=255)

                                                                                                                                                 gAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTc   <  4:775124/150‑1 (MQ=255)

                                                                                                                                                 gAGTACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCg  >  3:307782/1‑151 (MQ=255)

CTCATTGACGCGTCAGGCGGTACGTCTGGGATATTTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCGTTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCG  >  NC_000913/3932242‑3932537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`