BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,582,590	A→G	R153R (CGT→CGC)	hsdM ←	DNA methyltransferase M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,582,590	0	A	G	99.1%	398.9 / NA	112	R153R (CGT→CGC)	hsdM	DNA methyltransferase M
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (61/50); total (62/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

CGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGT  >  NC_000913/4582445‑4582734

  ccATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCgggg                                                                                                                                           >  1:234433/1‑151 (MQ=255)

    aTCAGGGTCGCCCAGATCTTTGGTTTGCGACTTAACATAGCGGTCGGTTTAAAACAAAAGGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGgcgc                                                                                                                                               <  2:644123/145‑1 (MQ=255)

    aTCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTg                                                                                                                                         >  3:223534/1‑151 (MQ=255)

       aaGGTCGCCCAGATCATTGTTTGGCGACTAAACAAGGCGGTGGGCTTAAATAAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAg                                                                                                                                      <  2:1489835/151‑1 (MQ=255)

       aaGGTCGCCCAGATCATTGGTTTGCGATTTAACATGGGGGTCGGCTTAAATAAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGcgcg                                                                                                                                              <  4:477042/143‑1 (MQ=255)

        aGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGt                                                                                                                                     >  2:696625/1‑151 (MQ=255)

           tcgtcCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACt                                                                                                                                  >  2:731896/1‑151 (MQ=255)

             gtcCAGATCTTTGGTTGGCGACTTAACATAGCGGTGGGCTTCAACAAAAAGGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACt                                                                                                                                  <  2:1244468/149‑1 (MQ=255)

             gtcCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTgg                                                                                                                                >  2:508498/1‑151 (MQ=255)

               ccAGATCTTTGTTTTGCGACTTAACATAGCGGTCGGCTTCAATAAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTg                                                                                                                                 <  4:110318/148‑1 (MQ=255)

               ccAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGcc                                                                                                                              >  2:1602032/1‑151 (MQ=255)

               ccAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATAAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGcc                                                                                                                              <  3:437302/151‑1 (MQ=255)

                  gATCTTTGTTTTGCGACTTAACATAGCGGTCGGCTTCAACCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGc                                                                                                                               <  1:141555/147‑1 (MQ=255)

                    tCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCAc                                                                                                                         >  4:1327582/1‑151 (MQ=255)

                     cATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCAcc                                                                                                                        >  3:385390/1‑151 (MQ=255)

                           tttGCGACTTAACATAGGGGCCGACTTAAACCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGa                                                                                                                     <  2:637839/148‑1 (MQ=255)

                             tGCGACTTAACATAGCGGTCGGCTTCAATCAAAACGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTgg                                                                                                                >  1:56504/1‑151 (MQ=255)

                              gCGACTTAACATGGCGGGGGGGGGAAAAGAGAAAGCCCTCCGTGGCTGCCGCCGGGGCGTGCCCCACTTCAAACAACAGAAGATTCAGCAGATGAATAATTGTTTTAATCAGCGGACGCGGGGTGAAGTACAGTCCTGCACCAGACTTgg                                                                                                                >  2:838695/1‑150 (MQ=255)

                               cGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGtt                                                                                                              >  2:840198/1‑151 (MQ=255)

                                gACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGttt                                                                                                             >  2:1180122/1‑151 (MQ=255)

                                 gtttAAAATAGGGGTCGGCTTAAATAAAAAACCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTc                                                                                                            <  2:680479/149‑1 (MQ=255)

                                 aCTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTc                                                                                                            >  3:321115/1‑151 (MQ=255)

                                   taagcatggggttCGGTTTAAATAAAAAACCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTc                                                                                                            <  2:983513/137‑1 (MQ=255)

                                     aaCATGGCTGTCGGTTTAAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCAt                                                                                                          <  1:127727/149‑1 (MQ=255)

                                     aaCATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTc                                                                                                        >  4:392880/1‑151 (MQ=255)

                                        aTAGCGGTCGGTTTCAATAAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTcgc                                                                                                      <  3:609/150‑1 (MQ=255)

                                           gCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGttc                                                                                                  >  3:403044/1‑151 (MQ=255)

                                              gTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGttcttc                                                                                               >  4:922309/1‑151 (MQ=255)

                                               tCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTtcttct                                                                                              >  4:640930/1‑151 (MQ=255)

                                                cGGCTTGAATCAAAAAGACCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCAGCACCAGACTTGCTTTCATTCGCGGTctgctg                                                                                             >  1:198336/1‑151 (MQ=255)

                                                   cTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCa                                                                                           >  4:711579/1‑150 (MQ=255)

                                                       aataaaAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACa                                                                                        <  2:1437679/149‑1 (MQ=255)

                                                       aataaaAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGc                                                                                      <  4:1215581/151‑1 (MQ=255)

                                                        atcaaAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGcc                                                                                     >  2:114686/1‑151 (MQ=255)

                                                             aaaCCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGAGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCg                                                                                <  1:187130/151‑1 (MQ=255)

                                                              aaGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGt                                                                               <  3:17082/151‑1 (MQ=255)

                                                               aGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGt                                                                               >  4:770707/1‑150 (MQ=255)

                                                                 cccGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTAc                                                                             >  2:505844/1‑150 (MQ=255)

                                                                    gccgTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACatat                                                                         >  4:1011205/1‑151 (MQ=255)

                                                                    gccgTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACatat                                                                         <  4:160292/151‑1 (MQ=255)

                                                                       gTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGc                                                                      >  2:325775/1‑151 (MQ=255)

                                                                       gTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGc                                                                      >  2:888710/1‑151 (MQ=255)

                                                                       gTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGc                                                                      >  4:280879/1‑151 (MQ=255)

                                                                       gTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGc                                                                      <  2:130605/151‑1 (MQ=255)

                                                                       gTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGc                                                                      <  2:1329104/151‑1 (MQ=255)

                                                                         aCCTGCAGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCg                                                                    >  3:407386/1‑151 (MQ=255)

                                                                          ccTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCg                                                                    <  2:955165/150‑1 (MQ=255)

                                                                          ccTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGa                                                                   <  3:336725/151‑1 (MQ=255)

                                                                           cTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCg                                                                    >  2:63547/1‑149 (MQ=255)

                                                                           cTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGCGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGa                                                                   >  4:1306488/1‑150 (MQ=255)

                                                                            tGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGa                                                                   >  2:190511/1‑149 (MQ=255)

                                                                            tGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGa                                                                   >  3:308665/1‑149 (MQ=255)

                                                                             gccgccGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAg                                                                 <  4:279292/150‑1 (MQ=255)

                                                                             gccgccGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGt                                                                >  4:820483/1‑151 (MQ=255)

                                                                               cgcctGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTc                                                               >  2:895360/1‑150 (MQ=255)

                                                                               cgccgGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCa                                                              <  4:207520/151‑1 (MQ=255)

                                                                               cgccgGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGACTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCa                                                              <  4:232494/151‑1 (MQ=255)

                                                                                gccgGGTCCTGCACCACTTCACGCGGCTGCGGATTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCa                                                              >  4:1650925/1‑150 (MQ=255)

                                                                                 ccgGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATc                                                            >  3:107907/1‑151 (MQ=255)

                                                                                 ccgGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATc                                                            >  2:546044/1‑151 (MQ=255)

                                                                                   gggTCCTGCACCACTTCACGCGGCTGCGGTTTCAGGAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATcgc                                                          <  4:446024/151‑1 (MQ=255)

                                                                                   gggTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATcgc                                                          >  3:125681/1‑151 (MQ=255)

                                                                                     gTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATcgcgc                                                        <  2:258852/151‑1 (MQ=255)

                                                                                      tCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACatat                                                                         >  1:35565/1‑133 (MQ=255)

                                                                                      tCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACatat                                                                         <  3:35567/133‑1 (MQ=255)

                                                                                      tCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATcgcgcg                                                       >  4:1136701/1‑151 (MQ=255)

                                                                                       ccTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGa                                                      <  2:241492/151‑1 (MQ=255)

                                                                                            accacTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTAc                                                 >  2:555653/1‑151 (MQ=255)

                                                                                             ccacTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTAcc                                                <  2:467065/151‑1 (MQ=255)

                                                                                               acTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGt                                              <  4:131113/151‑1 (MQ=255)

                                                                                                cTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTg                                             >  4:1521365/1‑151 (MQ=255)

                                                                                                  tCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTgcg                                           >  1:412056/1‑151 (MQ=255)

                                                                                                  tCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTgcg                                           >  4:880122/1‑151 (MQ=255)

                                                                                                   cACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTgcgc                                          <  2:840257/151‑1 (MQ=255)

                                                                                                   cACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTgcgc                                          <  4:164931/151‑1 (MQ=255)

                                                                                                    aCGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTgcgc                                          >  4:1158654/1‑150 (MQ=255)

                                                                                                      gcgGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACACATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGcc                                       >  2:187110/1‑151 (MQ=255)

                                                                                                       cgGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCg                                      >  4:1090595/1‑151 (MQ=255)

                                                                                                         gCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGtt                                    <  2:262361/151‑1 (MQ=255)

                                                                                                           tGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGt                                  >  4:468702/1‑151 (MQ=255)

                                                                                                                   cagcagATGAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTcc                          >  4:1266343/1‑151 (MQ=255)

                                                                                                                           gAATAATGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAAt                   <  1:88441/150‑1 (MQ=255)

                                                                                                                            aataatGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATcc                 <  1:237328/151‑1 (MQ=255)

                                                                                                                              taatGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCat               >  4:1306251/1‑151 (MQ=255)

                                                                                                                               aatGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCata              <  4:1454956/151‑1 (MQ=255)

                                                                                                                               aatGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCata              <  4:1590330/151‑1 (MQ=255)

                                                                                                                               aatGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCata              <  4:1251305/151‑1 (MQ=255)

                                                                                                                               aatGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCata              <  1:113098/151‑1 (MQ=255)

                                                                                                                                atGGTTTTAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCatat             >  2:949932/1‑151 (MQ=255)

                                                                                                                                     tttAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTg        >  3:267859/1‑151 (MQ=255)

                                                                                                                                       tAATCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGa       >  4:655003/1‑150 (MQ=255)

                                                                                                                                        aaTCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGAcc     >  2:390789/1‑151 (MQ=255)

                                                                                                                                        aaTCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGAcc     <  4:1214304/151‑1 (MQ=255)

                                                                                                                                        aaTCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGAcc     >  2:204155/1‑151 (MQ=255)

                                                                                                                                        aaTCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGAcc     >  2:816850/1‑151 (MQ=255)

                                                                                                                                          tCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCt         <  4:1401083/145‑1 (MQ=255)

                                                                                                                                          tCAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCt         >  2:1399332/1‑145 (MQ=255)

                                                                                                                                           cAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGt  <  2:769639/151‑1 (MQ=255)

                                                                                                                                           cAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGt  >  4:1143111/1‑151 (MQ=255)

                                                                                                                                           cAGCGGGCGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGt  <  2:710626/151‑1 (MQ=255)

CGCCATCAAGGTCGTCCAGATCATTGGTTTGCGACTTAACATAGCGGTCGGCTTCAATCAAAAAGCCCGCCGTACCTGCCGCCGGGTCCTGCACCACTTCACGCGGCTGCGGTTTCAGCAGATGAATAATGGTTTTAATCAGCGGACGCGGGGTGAAGTACTGGCCTGCACCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGT  >  NC_000913/4582445‑4582734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`