Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 455,102 A→G intergenic (+313/‑31) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913455,1020AG98.2% 414.3 / ‑2.3 113intergenic (+313/‑31)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (56/54);  total (58/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 113 total aligned reads displayed.

GTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAA  >  NC_000913/454955‑455244
                                                                                                                                                   |                                                                                                                                              
gTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCg                                                                                                                                             >  4:846907/1‑151 (MQ=255)
  tctcGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCg                                                                                                                                             >  1:182473/1‑149 (MQ=255)
     cGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAg                                                                                                                                           <  2:1595482/148‑1 (MQ=255)
     cGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCAc                                                                                                                                        >  1:245795/1‑151 (MQ=255)
     cGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCAc                                                                                                                                        >  4:1655338/1‑151 (MQ=255)
      gACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACt                                                                                                                                       >  2:21410/1‑151 (MQ=255)
          cATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACtgtg                                                                                                                                    <  4:896489/150‑1 (MQ=255)
          cATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACtgtg                                                                                                                                    >  2:1585398/1‑150 (MQ=255)
          cATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACtgtg                                                                                                                                    >  2:863955/1‑150 (MQ=255)
          cATAAAAGTGATGCCGCTATAATGCCGCGTCCTATCTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACtgt                                                                                                                                     <  4:429162/149‑1 (MQ=255)
            tAAAAGTGATGCCGCGATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAGCACTGTGAtt                                                                                                                                 >  4:1213189/1‑151 (MQ=255)
               aaGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGAttttt                                                                                                                              >  2:313193/1‑151 (MQ=255)
                aGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGAtttttt                                                                                                                             <  2:1117083/151‑1 (MQ=255)
                aGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGAtttttt                                                                                                                             <  2:176892/151‑1 (MQ=255)
                 gTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGAtttttt                                                                                                                             >  4:1087303/1‑150 (MQ=255)
                   gATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTg                                                                                                                            >  2:727628/1‑149 (MQ=255)
                    aTGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAgg                                                                                                                         <  4:1525277/151‑1 (MQ=255)
                    aTGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATGCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACATCCTGTGCGTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAgg                                                                                                                         <  1:329112/151‑1 (MQ=255)
                     tGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGt                                                                                                                        >  2:81289/1‑151 (MQ=255)
                      gCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTa                                                                                                                       <  2:996085/151‑1 (MQ=255)
                        cGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAAc                                                                                                                     >  4:1536644/1‑151 (MQ=255)
                         gCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACa                                                                                                                    <  4:1492138/151‑1 (MQ=255)
                            ataATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACaa                                                                                                                   <  4:1638166/149‑1 (MQ=255)
                            ataATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACa                                                                                                                    >  4:680133/1‑148 (MQ=255)
                             taATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACa                                                                                                                    >  2:1368174/1‑147 (MQ=255)
                              aaTGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATg                                                                                                               >  4:727006/1‑151 (MQ=255)
                               aTGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGc                                                                                                              >  4:475393/1‑151 (MQ=255)
                               aTGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGc                                                                                                              <  4:1271531/151‑1 (MQ=255)
                               aTGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGATAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGc                                                                                                              >  2:134402/1‑151 (MQ=255)
                                  ccGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCa                                                                                                             >  1:275353/1‑149 (MQ=255)
                                  ccGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCa                                                                                                             >  4:1175079/1‑149 (MQ=255)
                                    gcgTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGtt                                                                                                         <  4:491072/151‑1 (MQ=255)
                                      gTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTc                                                                                                       <  4:1393820/151‑1 (MQ=255)
                                        ccTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCa                                                                                                      <  4:1280881/150‑1 (MQ=255)
                                        ccTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAg                                                                                                     <  3:346405/151‑1 (MQ=255)
                                           aTTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTg                                                                                                  >  2:224959/1‑151 (MQ=255)
                                             ttGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGa                                                                                                 <  2:619524/150‑1 (MQ=255)
                                             ttGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGa                                                                                                 <  4:1119696/150‑1 (MQ=255)
                                             ttGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGa                                                                                                 >  3:325858/1‑150 (MQ=255)
                                             ttGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGa                                                                                                 >  3:324999/1‑150 (MQ=255)
                                                 aTGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAAcc                                                                                             >  4:885225/1‑150 (MQ=255)
                                                      ttCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCa                                                                                        <  1:386385/150‑1 (MQ=255)
                                                         gggATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGc                                                                                    >  4:792372/1‑151 (MQ=255)
                                                              gatTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTg                                                                                <  3:197434/150‑1 (MQ=255)
                                                                 tCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGgcc                                                                             <  2:1636272/150‑1 (MQ=255)
                                                                       aaCAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCgtgt                                                                      <  2:64979/151‑1 (MQ=255)
                                                                       aaCAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCgtg                                                                       <  3:171973/150‑1 (MQ=255)
                                                                          aGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAAc                                                                   <  1:95225/151‑1 (MQ=255)
                                                                          aGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAAc                                                                   <  4:360544/151‑1 (MQ=255)
                                                                           gggAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACg                                                                  <  2:908848/151‑1 (MQ=255)
                                                                           gggAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACg                                                                  <  4:651501/151‑1 (MQ=255)
                                                                           gggAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGAGTAACg                                                                  <  3:371568/151‑1 (MQ=255)
                                                                            ggAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGa                                                                 <  2:1051848/151‑1 (MQ=255)
                                                                             gAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGAt                                                                <  1:134541/151‑1 (MQ=255)
                                                                             gAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGAt                                                                <  4:1399056/151‑1 (MQ=255)
                                                                             gAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGAt                                                                <  4:1651365/151‑1 (MQ=255)
                                                                              aaTGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGAtt                                                               <  2:442875/151‑1 (MQ=255)
                                                                                   gattgattATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTa                                                           <  1:132489/150‑1 (MQ=255)
                                                                                    attgattATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATc                                                         >  4:534167/1‑151 (MQ=255)
                                                                                     ttgattATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATc                                                         <  2:860786/150‑1 (MQ=255)
                                                                                        gatctaAGAACATCAGGGTTCCGCGAAGCCAACAACCTGTGCCTGCGGGGAAAGAGTTGTCCGAGCACTGTGATTTTTTGAGGTAACAAGATCTAAGTTTCAGTTGAAACCACTCAAGGCCTTGGTCACCGTGTAACGAGTACTATc                                                         >  1:57681/5‑147 (MQ=255)
                                                                                        attATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCgct                                                      <  3:450568/150‑1 (MQ=255)
                                                                                          tataAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCgctgct                                                   >  2:677891/1‑151 (MQ=255)
                                                                                            taAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGctgctg                                                  >  2:843583/1‑150 (MQ=255)
                                                                                               gAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAg                                              <  3:5223/151‑1 (MQ=255)
                                                                                                 aCATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGTGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGc                                             >  2:305127/1‑150 (MQ=255)
                                                                                                    tCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCg                                         >  2:1155073/1‑151 (MQ=255)
                                                                                                    tCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCg                                         >  2:834665/1‑151 (MQ=255)
                                                                                                      ccGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCgag                                       <  2:590778/151‑1 (MQ=255)
                                                                                                           tCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGc                                  >  2:821508/1‑151 (MQ=255)
                                                                                                            ccGCGAAGCCAACAACCTGTGCTTGCGTGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCt                                 >  2:994829/1‑151 (MQ=255)
                                                                                                            ccGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCt                                 >  4:1301689/1‑151 (MQ=255)
                                                                                                             cgcgAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTg                                >  2:509848/1‑151 (MQ=255)
                                                                                                             cgcgAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTg                                <  3:63563/151‑1 (MQ=255)
                                                                                                              gcgAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGt                               >  2:1582959/1‑151 (MQ=255)
                                                                                                              gcgAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGt                               >  4:1368601/1‑151 (MQ=255)
                                                                                                                gAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGtt                              >  2:583633/1‑150 (MQ=255)
                                                                                                                gAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGtt                              >  3:96129/1‑150 (MQ=255)
                                                                                                                   gCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTaaaa                          <  2:781114/151‑1 (MQ=255)
                                                                                                                   gCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTaaaa                          <  2:1315147/151‑1 (MQ=255)
                                                                                                                   gCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTaaaa                          <  4:1091146/151‑1 (MQ=255)
                                                                                                                   gCCAACAACCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTaaaa                          <  4:1369878/151‑1 (MQ=255)
                                                                                                                      aacaacCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCg                       >  4:20082/1‑151 (MQ=255)
                                                                                                                       acaacCTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGa                      <  4:193193/151‑1 (MQ=255)
                                                                                                                           ccTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTg                  >  3:203020/1‑151 (MQ=255)
                                                                                                                           ccTGTGCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTACTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTg                  <  4:756573/151‑1 (MQ=255)
                                                                                                                               tgCTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTc               >  3:437494/1‑150 (MQ=255)
                                                                                                                                 cTTGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCaa             >  2:1445503/1‑150 (MQ=255)
                                                                                                                                  ttGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACg           >  4:230360/1‑151 (MQ=255)
                                                                                                                                   tGCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGt          >  1:161761/1‑151 (MQ=255)
                                                                                                                                    gCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGtt         <  3:116902/151‑1 (MQ=255)
                                                                                                                                    gCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGt          <  4:1187241/150‑1 (MQ=255)
                                                                                                                                    gCGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGt          <  1:103421/150‑1 (MQ=255)
                                                                                                                                     cGGGGTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTg        <  3:182487/151‑1 (MQ=255)
                                                                                                                                      ggggTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGc       >  1:389017/1‑151 (MQ=255)
                                                                                                                                       aggTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCg      >  2:933079/2‑151 (MQ=255)
                                                                                                                                        ggcaaGAGTTGGCCGCGCACTGCGATTTTTTGAGGCAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCg      >  2:1114825/4‑150 (MQ=255)
                                                                                                                                        ggTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGa     <  4:685860/151‑1 (MQ=255)
                                                                                                                                         gTAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGaa    <  3:330866/151‑1 (MQ=255)
                                                                                                                                          tAAGAGTTGGCCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGaa    >  2:336124/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
GTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAATGCTTTCGGGATGATTCTGGTAACAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAA  >  NC_000913/454955‑455244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: