Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 489,636 A→G H1034R (CAC→CGC)  mscK → mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913489,6360AG100.0% 418.9 / NA 117H1034R (CAC→CGC) mscKmechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (56/61);  total (56/61)

Only 100 of 119 total aligned reads displayed.

GCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAA  >  NC_000913/489488‑489780
                                                                                                                                                    |                                                                                                                                                
gCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGcgcg                                                                                                                                                <  4:508724/151‑1 (MQ=255)
 cGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGcgcg                                                                                                                                                >  2:1476252/1‑150 (MQ=255)
 cGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGa                                                                                                                                               <  4:263953/151‑1 (MQ=255)
  gTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGaa                                                                                                                                              <  1:21275/151‑1 (MQ=255)
  gTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGa                                                                                                                                               >  3:471692/1‑150 (MQ=255)
   tttGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAAc                                                                                                                                             >  2:1336597/1‑151 (MQ=255)
   tttGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAAc                                                                                                                                             >  2:702011/1‑151 (MQ=255)
   tttGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGGCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTAATGAAGGCGGCGGCTGAGCACCCAAGGGTGATGCGCGAAc                                                                                                                                             >  4:210627/1‑151 (MQ=255)
    ttGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAAc                                                                                                                                             <  3:113636/150‑1 (MQ=255)
     tGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAAcc                                                                                                                                            >  4:638546/1‑150 (MQ=255)
      gTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCa                                                                                                                                           <  2:520729/150‑1 (MQ=255)
         aCCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGc                                                                                                                                       <  1:386722/151‑1 (MQ=255)
         aCCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCAAACCAATg                                                                                                                                        <  2:193578/150‑1 (MQ=255)
          ccGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGcc                                                                                                                                      <  4:552741/151‑1 (MQ=255)
            gAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCgg                                                                                                                                    <  2:743810/151‑1 (MQ=255)
               cGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAg                                                                                                                                 <  2:1594588/151‑1 (MQ=255)
                   tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGtctt                                                                                                                             >  3:237278/1‑151 (MQ=255)
                    gATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGtctt                                                                                                                             <  2:510836/150‑1 (MQ=255)
                     aTCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGtcttct                                                                                                                           <  1:125481/151‑1 (MQ=255)
                       cAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTcttctt                                                                                                                          <  4:1377545/150‑1 (MQ=255)
                           tGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACgg                                                                                                                     >  4:704160/1‑151 (MQ=255)
                           tGGTCGTTGACTGACACTACTACGCGTCTGGAGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGAGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACgg                                                                                                                     >  1:36067/1‑151 (MQ=255)
                               cGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCAtt                                                                                                                 >  2:485493/1‑151 (MQ=255)
                               cGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCAt                                                                                                                  <  2:1484038/150‑1 (MQ=255)
                                 ttGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCAttt                                                                                                                <  2:894013/150‑1 (MQ=255)
                                  tgactgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTg                                                                                                               <  4:1144656/150‑1 (MQ=255)
                                      tgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGcc                                                                                                          >  1:346192/1‑151 (MQ=255)
                                       gacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGcc                                                                                                          <  4:1338271/150‑1 (MQ=255)
                                        acacTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAg                                                                                                        <  4:89011/151‑1 (MQ=255)
                                             actacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACg                                                                                                    <  1:157839/150‑1 (MQ=255)
                                             actacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGt                                                                                                   >  4:608609/1‑151 (MQ=255)
                                                acGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTgg                                                                                                <  2:414587/151‑1 (MQ=255)
                                                acGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGAAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGtt                                                                                                  <  1:449251/149‑1 (MQ=255)
                                                   cgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATc                                                                                             <  4:494941/151‑1 (MQ=255)
                                                    gTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATc                                                                                             >  2:171658/1‑150 (MQ=255)
                                                     tCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCAt                                                                                           >  3:431074/1‑151 (MQ=255)
                                                        ggTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATg                                                                                          <  4:1123383/149‑1 (MQ=255)
                                                        ggTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATg                                                                                          <  4:1241803/149‑1 (MQ=255)
                                                         gTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGc                                                                                       <  2:882932/151‑1 (MQ=255)
                                                              ccGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCg                                                                                   <  4:397367/150‑1 (MQ=255)
                                                              ccGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCg                                                                                   <  2:1209033/150‑1 (MQ=255)
                                                               cGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTc                                                                                 <  1:416609/151‑1 (MQ=255)
                                                                 tctcGGCGTGGCCTATGGCTCCGGTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTg                                                                               >  2:1417101/1‑151 (MQ=255)
                                                                   tcGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGt                                                                              >  4:123975/1‑150 (MQ=255)
                                                                      gCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTAtgt                                                                          >  2:84886/1‑151 (MQ=255)
                                                                      gCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTAtgt                                                                          <  4:1342446/151‑1 (MQ=255)
                                                                       cGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTAtgtg                                                                         >  2:1229907/1‑151 (MQ=255)
                                                                          ggCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGATCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCg                                                                       <  3:465364/150‑1 (MQ=255)
                                                                          ggCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCg                                                                       <  2:687863/150‑1 (MQ=255)
                                                                          ggCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGt                                                                      >  2:386182/1‑151 (MQ=255)
                                                                           gCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTg                                                                     <  4:1361392/151‑1 (MQ=255)
                                                                            ccTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGa                                                                    <  3:337379/151‑1 (MQ=255)
                                                                             cTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGa                                                                    >  3:14440/1‑150 (MQ=255)
                                                                               aTGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACt                                                                 <  3:421011/151‑1 (MQ=255)
                                                                               aTGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACt                                                                 <  2:955257/151‑1 (MQ=255)
                                                                                tGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTg                                                                >  3:186442/1‑151 (MQ=255)
                                                                                tGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGACGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTg                                                                >  4:565484/1‑151 (MQ=255)
                                                                                 ggCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGc                                                               >  2:1047378/1‑151 (MQ=255)
                                                                                  gCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCg                                                              <  4:915498/151‑1 (MQ=255)
                                                                                  gCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCg                                                              <  4:554908/151‑1 (MQ=255)
                                                                                      cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCAAGCACGTTGGATCATGAGCTGCGTCTGAATGTGCGTGAACTGCGTGAc                                                          >  1:454593/1‑151 (MQ=255)
                                                                                       gATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGAcc                                                         >  2:1523836/1‑151 (MQ=255)
                                                                                            ggAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCg                                                        <  2:1331323/147‑1 (MQ=255)
                                                                                            ggAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTa                                                      <  2:1284419/149‑1 (MQ=255)
                                                                                            ggAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTa                                                      <  4:1310810/149‑1 (MQ=255)
                                                                                              aaaaaGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCg                                                  >  2:1330850/1‑151 (MQ=255)
                                                                                              aaaaaGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCg                                                  >  4:578063/1‑151 (MQ=255)
                                                                                               aaaaGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGt                                                 >  3:233057/1‑151 (MQ=255)
                                                                                               aaaaGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGt                                                 >  1:162838/1‑151 (MQ=255)
                                                                                                  aGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACt                                              <  4:175014/151‑1 (MQ=255)
                                                                                                   gTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTg                                             >  4:244706/1‑151 (MQ=255)
                                                                                                    tGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGt                                            >  1:396692/1‑151 (MQ=255)
                                                                                                     gCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGt                                            <  2:107586/150‑1 (MQ=255)
                                                                                                      cGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCg                                          >  2:1517403/1‑151 (MQ=255)
                                                                                                      cGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCg                                          <  1:36676/151‑1 (MQ=255)
                                                                                                         aaaGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGGGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATg                                       >  1:115741/1‑151 (MQ=255)
                                                                                                         aaaGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATg                                       <  2:178111/151‑1 (MQ=255)
                                                                                                          aaGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGa                                      <  3:38067/151‑1 (MQ=255)
                                                                                                           aGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCg                                          >  1:383315/1‑146 (MQ=255)
                                                                                                           aGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATg                                       >  4:635034/1‑149 (MQ=255)
                                                                                                           aGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAg                                     <  4:1450629/151‑1 (MQ=255)
                                                                                                              gtTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCt                                   <  4:1014713/150‑1 (MQ=255)
                                                                                                                tACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGa                                 >  1:356581/1‑150 (MQ=255)
                                                                                                                       ggcggcGACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACt                         >  2:1148933/1‑151 (MQ=255)
                                                                                                                         tggcgACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTAt                       >  2:393943/2‑151 (MQ=255)
                                                                                                                           gcgACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCg                     >  4:1476464/1‑151 (MQ=255)
                                                                                                                            cgACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCg                     >  2:1147370/1‑150 (MQ=255)
                                                                                                                             gACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGAt                   >  2:116672/1‑151 (MQ=255)
                                                                                                                             gACTGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGAt                   <  2:720679/151‑1 (MQ=255)
                                                                                                                                tGAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATc                  >  4:1099863/1‑149 (MQ=255)
                                                                                                                                 gAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAg                <  4:784854/150‑1 (MQ=255)
                                                                                                                                 gAGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGCAGTCGTACTGTCGATGAGCTGAACCTTACTATCGATCa                 <  2:210287/149‑1 (MQ=255)
                                                                                                                                  aGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCt              >  2:1279285/1‑151 (MQ=255)
                                                                                                                                  aGCACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCt              <  4:630403/151‑1 (MQ=255)
                                                                                                                                    cACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCtgt            <  4:172307/151‑1 (MQ=255)
                                                                                                                                    cACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCtgt            >  4:1079074/1‑151 (MQ=255)
                                                                                                                                    cACCCAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCtgt            <  3:202229/151‑1 (MQ=255)
                                                                                                                                      cccAAGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGc          >  4:1603473/1‑151 (MQ=255)
                                                                                                                                         aaGGGTGATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGt       >  4:855184/1‑151 (MQ=255)
                                                                                                                                               gATGCGCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGaaaa  <  2:607779/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAA  >  NC_000913/489488‑489780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑