Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 667,766 T→C Y151C (TAC→TGC)  mrdA ← penicillin‑binding protein 2, transpeptidase involved in peptidoglycan synthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913667,7660TC100.0% 507.3 / NA 133Y151C (TAC→TGC) mrdApenicillin‑binding protein 2, transpeptidase involved in peptidoglycan synthesis
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (69/64);  total (69/64)

Only 100 of 133 total aligned reads displayed.

CGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTCC  >  NC_000913/667618‑667912
                                                                                                                                                    |                                                                                                                                                  
cGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCAc                                                                                                                                                  <  3:377103/151‑1 (MQ=255)
  cATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTg                                                                                                                                                >  1:48720/1‑151 (MQ=255)
      gTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTg                                                                                                                                            >  1:12460/1‑151 (MQ=255)
      gTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTg                                                                                                                                            <  3:59544/151‑1 (MQ=255)
        tGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGAc                                                                                                                                          >  2:674183/1‑151 (MQ=255)
         ggCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACg                                                                                                                                         >  4:864309/1‑151 (MQ=255)
         ggCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACg                                                                                                                                         >  4:490912/1‑151 (MQ=255)
              gTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCaaa                                                                                                                                    <  2:1268908/151‑1 (MQ=255)
                  gCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCg                                                                                                                                 <  4:759128/150‑1 (MQ=255)
                   ccGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAg                                                                                                                               <  4:1027657/151‑1 (MQ=255)
                     gTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCt                                                                                                                             <  3:124872/151‑1 (MQ=255)
                       cATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTa                                                                                                                            <  2:1061382/150‑1 (MQ=255)
                          tattCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTg                                                                                                                        >  2:1189481/1‑151 (MQ=255)
                               aGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCTAGCTACTTGTACtt                                                                                                                   >  4:95044/1‑151 (MQ=255)
                                   gTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCgg                                                                                                                <  2:1282176/150‑1 (MQ=255)
                                   gTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGt                                                                                                               >  4:147279/1‑151 (MQ=255)
                                   gTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGt                                                                                                               >  4:454053/1‑151 (MQ=255)
                                     tCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTc                                                                                                              <  4:639139/150‑1 (MQ=255)
                                     tCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACCTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTc                                                                                                              >  3:367969/1‑150 (MQ=255)
                                      cGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAg                                                                                                            <  4:669582/151‑1 (MQ=255)
                                      cGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAg                                                                                                            <  3:67099/151‑1 (MQ=255)
                                       gACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAgg                                                                                                           >  2:1585815/1‑151 (MQ=255)
                                       gACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAgg                                                                                                           >  1:203779/1‑151 (MQ=255)
                                       gACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAgg                                                                                                           >  2:1502760/1‑151 (MQ=255)
                                       gACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAgg                                                                                                           >  4:326185/1‑151 (MQ=255)
                                        aCGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGt                                                                                                          >  2:728926/1‑151 (MQ=255)
                                        aCGTCTTTAGCGTTGATTTTCGACACATAGCCGATGACTTGGGTCAACGGCGAACCTTTAGGATAGTTTTGACATTTATACCCCGCAACCCCGACACCCCTAAAACGGCCCTGACTGTCTTCCAAGCGAGCTCCTTCTCCTTCgc                                                                                                                <  2:757549/145‑2 (MQ=255)
                                         cGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGtt                                                                                                         <  4:907264/151‑1 (MQ=255)
                                              ttATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGt                                                                                                      <  4:675109/149‑1 (MQ=255)
                                              ttATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGt                                                                                                      <  4:939263/149‑1 (MQ=255)
                                               tATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGtttt                                                                                                   >  4:120498/1‑151 (MQ=255)
                                                aTCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGtt                                                                                                     <  3:295619/148‑1 (MQ=255)
                                                 tCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTc                                                                                                  >  3:336350/1‑150 (MQ=255)
                                                  cGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCAc                                                                                                >  4:430293/1‑151 (MQ=255)
                                                   gTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCAcc                                                                                               >  4:935474/1‑151 (MQ=255)
                                                     tGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCg                                                                                              <  2:1227979/150‑1 (MQ=255)
                                                       aTTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGa                                                                                            >  4:419254/1‑150 (MQ=255)
                                                         tttCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAAt                                                                                          >  3:173452/1‑150 (MQ=255)
                                                           tCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATag                                                                                        <  3:284825/150‑1 (MQ=255)
                                                             gACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCg                                                                                                                 >  1:260929/1‑123 (MQ=255)
                                                               cacaTAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTg                                                                                   <  2:15002/151‑1 (MQ=255)
                                                                acaTAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTg                                                                                   >  4:1019137/1‑150 (MQ=255)
                                                                acaTAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGa                                                                                  <  2:1234979/151‑1 (MQ=255)
                                                                  aTAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGaa                                                                                 >  2:454802/1‑150 (MQ=255)
                                                                     gCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACgg                                                                             <  2:556885/151‑1 (MQ=255)
                                                                       cGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGgtg                                                                           <  1:334790/151‑1 (MQ=255)
                                                                       cGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGgtg                                                                           >  2:1277501/1‑151 (MQ=255)
                                                                         aTGACGTGGGTCAACGCCGAACCGGAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGtgtg                                                                         <  2:378450/151‑1 (MQ=255)
                                                                           gACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGaa                                                                       <  4:885625/151‑1 (MQ=255)
                                                                           gACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGa                                                                        >  4:1174522/1‑150 (MQ=255)
                                                                            aCGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAAc                                                                      <  2:772155/151‑1 (MQ=255)
                                                                                gggTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTgc                                                                  >  4:844580/1‑151 (MQ=255)
                                                                                    cAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTgcgcgc                                                              >  4:1301373/1‑151 (MQ=255)
                                                                                    cAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTgcgcg                                                               <  2:606874/150‑1 (MQ=255)
                                                                                      aCGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGctc                                                            >  2:510200/1‑151 (MQ=255)
                                                                                       cGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGctct                                                           >  4:17891/1‑151 (MQ=255)
                                                                                           gAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAATCGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTc                                                       <  1:468419/151‑1 (MQ=255)
                                                                                           gAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTc                                                       >  3:385156/1‑151 (MQ=255)
                                                                                           gAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTc                                                       <  1:271300/151‑1 (MQ=255)
                                                                                            aaCCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCg                                                      <  2:1198198/151‑1 (MQ=255)
                                                                                              ccGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGa                                                    <  3:219596/151‑1 (MQ=255)
                                                                                               cGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGa                                                    >  4:396265/1‑150 (MQ=255)
                                                                                               cGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGACAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGa                                                    >  4:1575549/1‑150 (MQ=255)
                                                                                                gTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAAt                                                  <  2:252160/151‑1 (MQ=255)
                                                                                                gTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAAt                                                  <  4:483310/151‑1 (MQ=255)
                                                                                                  aaGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATgc                                                <  4:572929/151‑1 (MQ=255)
                                                                                                     gATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATgcagc                                             >  2:591998/1‑151 (MQ=255)
                                                                                                     gATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATgcagc                                             <  4:729905/151‑1 (MQ=255)
                                                                                                     gATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATgcagc                                             <  4:893844/151‑1 (MQ=255)
                                                                                                        aGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCaa                                           <  4:634194/150‑1 (MQ=255)
                                                                                                           aaCGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATc                                       >  4:24219/1‑151 (MQ=255)
                                                                                                           aaCGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATc                                       >  4:1568723/1‑151 (MQ=255)
                                                                                                            acgacgTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCg                                      >  2:1248616/1‑151 (MQ=255)
                                                                                                            acgacgTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCg                                      <  1:193206/151‑1 (MQ=255)
                                                                                                             cgacgTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGGGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGt                                     >  4:199855/1‑151 (MQ=255)
                                                                                                              gacgTTTATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTc                                    >  2:671468/1‑151 (MQ=255)
                                                                                                                   ttATAGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCgg                               >  3:257885/1‑151 (MQ=255)
                                                                                                                     ataGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTc                             >  4:342597/1‑151 (MQ=255)
                                                                                                                       aGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCa                            <  2:972206/150‑1 (MQ=255)
                                                                                                                       aGCCTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAg                           <  3:276286/151‑1 (MQ=255)
                                                                                                                         ccTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTc                             >  2:910234/1‑147 (MQ=255)
                                                                                                                          cTTTAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTc                             >  2:169132/1‑146 (MQ=255)
                                                                                                                           tttAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCt                       >  4:212570/1‑151 (MQ=255)
                                                                                                                             tAACTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTa                      <  2:452041/150‑1 (MQ=255)
                                                                                                                               aCTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTAcc                    >  2:426955/1‑150 (MQ=255)
                                                                                                                               aCTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTAcc                    >  2:13596/1‑150 (MQ=255)
                                                                                                                               aCTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCa                   <  2:524689/151‑1 (MQ=255)
                                                                                                                                cTTCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCAc                  >  4:172455/1‑151 (MQ=255)
                                                                                                                                 ttCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCAc                  >  4:1304614/1‑150 (MQ=255)
                                                                                                                                 ttCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACg                 >  2:944719/1‑151 (MQ=255)
                                                                                                                                  tCGACACCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCAc                  <  4:314982/149‑1 (MQ=255)
                                                                                                                                      cacCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTgcg            <  2:648375/151‑1 (MQ=255)
                                                                                                                                      cacCCGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTgcg            <  4:1351386/151‑1 (MQ=255)
                                                                                                                                        cccGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCa          <  4:1233876/151‑1 (MQ=255)
                                                                                                                                         ccGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCaa         <  4:218154/151‑1 (MQ=255)
                                                                                                                                         ccGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCa          >  2:1682243/1‑150 (MQ=255)
                                                                                                                                          cGGAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCaaa        <  2:863185/151‑1 (MQ=255)
                                                                                                                                           ggAAAACGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCaa         >  2:759960/1‑149 (MQ=255)
                                                                                                                                              aaaCGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGt    >  4:1025476/1‑151 (MQ=255)
                                                                                                                                                 cGGCACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTcc  >  1:105241/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGCATAGTTGGCCAGTTTGCCGTCATTATTCAGGCGTTCGACGTCTTTATCGTTGATTTTCGACACATAGCCGATGACGTGGGTCAACGCCGAACCGTAAGGATAGTAACGACGTTTATAGCCTTTAACTTCGACACCCGGAAAACGGTACTGATTGACGGCAAAGCGAGCTACTTGTACTTCGGTCAGGTTAGTTTTCACCGGAATAGAGGTGAAACGGTGTGAACGTGCGCGCTCTTTTCGGAATGCAGCAATATCGTCATCGGTCAGATCTACCACGCTGCGCAAAGCGTCC  >  NC_000913/667618‑667912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑