Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	699,271	T→C	intergenic (‑94/+303)	asnB ← / ← umpH	asparagine synthetase B/UMP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	699,271	0	T	C	100.0%	350.2 / NA	95	intergenic (‑94/+303)	asnB/umpH	asparagine synthetase B/UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/57);  total (38/57)

ACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTT  >  NC_000913/699124‑699418                                                                                                                                                    |                                                                                                                                                    aCGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGaa                                                                                                                                                  <  3:390213/151‑1 (MQ=255)  cGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGaaa                                                                                                                                                 <  4:712307/151‑1 (MQ=255)     aGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGaaaaaa                                                                                                                                              <  4:857664/151‑1 (MQ=255)     aGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGaaaaaa                                                                                                                                              <  4:1375373/151‑1 (MQ=255)      gCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAGAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAAt                                                                                                                                             <  2:291323/151‑1 (MQ=255)      gCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAAt                                                                                                                                             <  1:97205/151‑1 (MQ=255)        tCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATa                                                                                                                                            >  2:337394/1‑150 (MQ=255)          aaCTGCGTCTGTTTTGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATaaaa                                                                                                                                         <  1:28893/151‑1 (MQ=255)             tGCGTCTGTTTTGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAAc                                                                                                                                      >  2:539617/1‑151 (MQ=255)              gCGTCTGTTTTGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAAc                                                                                                                                      <  2:703247/150‑1 (MQ=255)              gCGTCTGTTTTGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACg                                                                                                                                     <  2:658418/151‑1 (MQ=255)                    gTTTTGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAAt                                                                                                                                 <  2:44697/149‑1 (MQ=255)                    gTTTTGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATgg                                                                                                                               <  2:846607/151‑1 (MQ=255)                      tttGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGt                                                                                                                              <  4:1320916/150‑1 (MQ=255)                        tGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGa                                                                                                                            <  4:118123/150‑1 (MQ=255)                         gATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGAtt                                                                                                                          <  2:711626/151‑1 (MQ=255)                          ttatCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTg                                                                                                                         >  2:133357/2‑151 (MQ=255)                          atatCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTg                                                                                                                         >  4:1092533/1‑151 (MQ=255)                            atCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTc                                                                                                                       <  2:257812/151‑1 (MQ=255)                              cGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCg                                                                                                                      <  2:424815/150‑1 (MQ=255)                              cGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCg                                                                                                                      <  4:879327/150‑1 (MQ=255)                              cGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGa                                                                                                                     <  2:978562/151‑1 (MQ=255)                                aaTACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGAtt                                                                                                                   <  2:1112157/151‑1 (MQ=255)                                    cGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTg                                                                                                               >  2:929154/1‑151 (MQ=255)                                     gCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGa                                                                                                              <  4:1526916/151‑1 (MQ=255)                                        aaaaaTTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGaaaa                                                                                                           <  4:1622751/151‑1 (MQ=255)                                        aaaaaTTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGaaaa                                                                                                           <  2:1486737/151‑1 (MQ=255)                                          aaaTTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGaaaaaa                                                                                                         <  4:1376562/151‑1 (MQ=255)                                              tGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTc                                                                                                     >  2:592231/1‑151 (MQ=255)                                                aaCACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTg                                                                                                   >  4:645185/1‑151 (MQ=255)                                                  cacaTAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGgt                                                                                                 >  4:1129663/1‑151 (MQ=255)                                                   acaTAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGgtg                                                                                                <  2:1517790/151‑1 (MQ=255)                                                       aaCCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGa                                                                                             <  2:1048684/150‑1 (MQ=255)                                                        aCCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAgg                                                                                           >  4:1101550/1‑151 (MQ=255)                                                         ccTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAgg                                                                                           >  4:1320289/1‑150 (MQ=255)                                                         ccTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGAAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAgg                                                                                           >  2:1157886/1‑150 (MQ=255)                                                            tctcCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGatat                                                                                       >  4:1266777/1‑151 (MQ=255)                                                            tctcCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGata                                                                                        <  4:917991/150‑1 (MQ=255)                                                            tctcCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGata                                                                                        <  2:901637/150‑1 (MQ=255)                                                            tctcCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGat                                                                                         <  2:942129/149‑1 (MQ=255)                                                            tctcCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGat                                                                                         <  2:1608698/149‑1 (MQ=255)                                                             ctcCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGatat                                                                                       >  4:692181/1‑150 (MQ=255)                                                               ccGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGatata                                                                                      <  1:462395/149‑1 (MQ=255)                                                               ccGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATAtatt                                                                                    <  2:526913/151‑1 (MQ=255)                                                                    aaCCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATAtatttatt                                                                                <  3:68982/150‑1 (MQ=255)                                                                    aaCCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATAtatttatt                                                                                <  4:691095/150‑1 (MQ=255)                                                                    aaCCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATtg                                                                               >  2:621748/1‑151 (MQ=255)                                                                    aaCCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATtg                                                                               <  2:438550/151‑1 (MQ=255)                                                                       cTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATtgatg                                                                            >  4:433091/1‑151 (MQ=255)                                                                        tGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATtgatg                                                                            >  3:173132/1‑150 (MQ=255)                                                                         ggTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATtgatga                                                                           >  1:242912/1‑150 (MQ=255)                                                                         ggTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATtgatga                                                                           >  2:347173/1‑150 (MQ=255)                                                                         ggTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATtgatga                                                                           >  2:1671807/1‑150 (MQ=255)                                                                         ggTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATtgatg                                                                            <  1:456469/149‑1 (MQ=255)                                                                          gTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAAt                                                                         >  4:1329372/1‑151 (MQ=255)                                                                            aTTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCg                                                                       <  4:214750/151‑1 (MQ=255)                                                                            aTTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCg                                                                       <  2:129390/151‑1 (MQ=255)                                                                              ttgttgCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGa                                                                      <  3:444063/150‑1 (MQ=255)                                                                              ttgttgCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGAt                                                                     >  2:725662/1‑151 (MQ=255)                                                                                 ttgCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAAt                                                                  >  4:1350771/1‑151 (MQ=255)                                                                                    cTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAActtt                                                               >  4:811306/1‑151 (MQ=255)                                                                                      tgttgtGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAAtttt                                                               <  4:473936/149‑1 (MQ=255)                                                                                       gttgtGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAAttttt                                                              >  4:628581/1‑149 (MQ=255)                                                                                       gttgtGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTa                                                             <  2:328098/150‑1 (MQ=255)                                                                                           tgtTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCggg                                                        >  4:977582/1‑151 (MQ=255)                                                                                           tgtTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCggg                                                        >  1:94082/1‑151 (MQ=255)                                                                                            gtTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGt                                                       <  4:258993/151‑1 (MQ=255)                                                                                               tGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGtttt                                                    >  2:1206300/1‑151 (MQ=255)                                                                                               tGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGtttt                                                    >  2:959379/1‑151 (MQ=255)                                                                                                gCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGtttt                                                    <  2:1327711/150‑1 (MQ=255)                                                                                                gCTTGTTTAAAAAAATGCCGCAAAGCAACACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGtttt                                                    <  2:1137924/150‑1 (MQ=255)                                                                                                   tGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTAtt                                                 <  2:890992/150‑1 (MQ=255)                                                                                                    gTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTg                                                >  1:99758/1‑150 (MQ=255)                                                                                                       tAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAAt                                             <  2:1235671/150‑1 (MQ=255)                                                                                                        aaaaaaaTGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATg                                            <  4:911031/150‑1 (MQ=255)                                                                                                         aaaaaaTGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGtt                                          >  4:240345/1‑151 (MQ=255)                                                                                                           aaaaTGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGttt                                         >  1:439568/1‑150 (MQ=255)                                                                                                           aaaaTGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTta                                        <  4:133582/151‑1 (MQ=255)                                                                                                              aTGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTatat                                     >  4:1303033/1‑151 (MQ=255)                                                                                                               ttccGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATAtt                                    >  3:298477/3‑151 (MQ=255)                                                                                                                gCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCAAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATAttt                                   <  4:1525969/151‑1 (MQ=255)                                                                                                                  cGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATAtttt                                  >  4:1635532/1‑150 (MQ=255)                                                                                                                      aaGCAGCACTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACggg                             >  3:41273/1‑151 (MQ=255)                                                                                                                        gcagcaCTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACggggg                           <  4:1291978/151‑1 (MQ=255)                                                                                                                            caCTGTGCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCaa                       <  4:110231/151‑1 (MQ=255)                                                                                                                                gtgCGCAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTg                   >  4:1312050/1‑151 (MQ=255)                                                                                                                                  gcgcAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGc                  <  4:540365/150‑1 (MQ=255)                                                                                                                                  gcgcAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCt                 <  4:659420/151‑1 (MQ=255)                                                                                                                                   cgcAAGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTg                <  2:644314/151‑1 (MQ=255)                                                                                                                                      aaGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGAc              <  4:1637199/150‑1 (MQ=255)                                                                                                                                       aGCGATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACaa            <  1:241858/151‑1 (MQ=255)                                                                                                                                          taTTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGt         >  2:703832/2‑151 (MQ=255)                                                                                                                                          gATTTGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGt         <  2:983571/151‑1 (MQ=255)                                                                                                                                              tGGCGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCg      <  3:75069/150‑1 (MQ=255)                                                                                                                                               ggcggcGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGAt    >  4:1015364/1‑151 (MQ=255)                                                                                                                                               ggcggcGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGAGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGa     <  2:1660819/150‑1 (MQ=255)                                                                                                                                                 cGGCGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGAttt  >  4:1231172/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ACGCAGCTCAACTGCGTCTGTTTTGATATCGAATACGCCAAAAATTGAACACATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTT  >  NC_000913/699124‑699418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑