Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 756,306 A→G T134A (ACT→GCT)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,3060AG100.0% 334.5 / NA 93T134A (ACT→GCT) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/47);  total (46/47)

ATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCAC  >  NC_000913/756159‑756453
                                                                                                                                                   |                                                                                                                                                   
aTTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTg                                                                                                                                                  >  4:838733/1‑151 (MQ=255)
aTTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTg                                                                                                                                                  <  4:543466/151‑1 (MQ=255)
       atatGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGgcagc                                                                                                                                           <  2:57133/151‑1 (MQ=255)
        tatGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGgc                                                                                                                                              >  3:99810/1‑147 (MQ=255)
        tatGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGgc                                                                                                                                              <  1:99796/147‑1 (MQ=255)
         atGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGgcagc                                                                                                                                           >  4:1168059/1‑149 (MQ=255)
               aaaaCCGGGCCGGAAGCGTTTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTg                                                                                                                                      <  2:907694/148‑1 (MQ=255)
               aaaaCCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGAc                                                                                                                                    <  2:1222214/150‑1 (MQ=255)
                aaaCCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCg                                                                                                                                  <  4:1346758/151‑1 (MQ=255)
                aaaCCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCg                                                                                                                                  <  2:122714/151‑1 (MQ=255)
                aaaCCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCg                                                                                                                                  <  1:271035/151‑1 (MQ=255)
                aaaCCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCg                                                                                                                                  <  4:767087/151‑1 (MQ=255)
                 aaCCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGt                                                                                                                                 <  4:1685625/151‑1 (MQ=255)
                 aaCCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGt                                                                                                                                 <  4:938066/151‑1 (MQ=255)
                  aCCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGt                                                                                                                                 >  4:68023/1‑150 (MQ=255)
                   ccGGGCCGGAAGCGATTCTGGAATCCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTAc                                                                                                                               <  1:27760/151‑1 (MQ=255)
                   ccGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTAc                                                                                                                               >  4:508967/1‑151 (MQ=255)
                    cGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTAcc                                                                                                                              >  2:599208/1‑151 (MQ=255)
                     gggCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCg                                                                                                                             <  4:137466/151‑1 (MQ=255)
                     gggCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCg                                                                                                                             >  3:199810/1‑151 (MQ=255)
                      ggCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCgg                                                                                                                            >  3:372559/1‑151 (MQ=255)
                        ccGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTc                                                                                                                          <  4:1240333/151‑1 (MQ=255)
                          ggAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCAc                                                                                                                        >  1:305229/1‑151 (MQ=255)
                          ggAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCAc                                                                                                                        <  3:111144/151‑1 (MQ=255)
                           gAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACg                                                                                                                       <  1:148276/151‑1 (MQ=255)
                            aaGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGc                                                                                                                      >  1:393418/1‑151 (MQ=255)
                              gCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCa                                                                                                                     <  4:1298116/150‑1 (MQ=255)
                              gCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCa                                                                                                                     <  1:9480/150‑1 (MQ=255)
                               tgATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACt                                                                                                                   >  1:112844/2‑151 (MQ=255)
                                gATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTg                                                                                                                  <  4:1089874/151‑1 (MQ=255)
                                  ttCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGtt                                                                                                                >  2:1122587/1‑151 (MQ=255)
                                   tCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGtt                                                                                                                <  4:1142240/150‑1 (MQ=255)
                                   tCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTg                                                                                                               >  4:96028/1‑151 (MQ=255)
                                   tCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTg                                                                                                               >  2:730288/1‑151 (MQ=255)
                                   tCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTg                                                                                                               >  2:653697/1‑151 (MQ=255)
                                      ggAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGca                                                                                                             <  3:308688/150‑1 (MQ=255)
                                      ggAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGca                                                                                                             <  4:689182/150‑1 (MQ=255)
                                             gAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCttt                                                                                                     >  4:74713/1‑151 (MQ=255)
                                              aaCACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTa                                                                                                    <  4:1079300/151‑1 (MQ=255)
                                                cacaTGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATc                                                                                                  <  2:288844/151‑1 (MQ=255)
                                                 acaTGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATc                                                                                                  >  1:106574/1‑150 (MQ=255)
                                                 acaTGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATc                                                                                                  >  2:798558/1‑150 (MQ=255)
                                                   aTGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATcagc                                                                                               <  2:979037/151‑1 (MQ=255)
                                                   aTGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGAACTGTTGCACACGCTTTATca                                                                                                 >  2:957761/1‑149 (MQ=255)
                                                    tGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATcagc                                                                                               >  2:1079964/1‑150 (MQ=255)
                                                     gggCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATcagca                                                                                              <  2:838233/150‑1 (MQ=255)
                                                      ggCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGa                                                                                            <  3:393435/151‑1 (MQ=255)
                                                         cTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAAcc                                                                                         >  2:196733/1‑151 (MQ=255)
                                                           gCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTg                                                                                       >  3:218599/1‑151 (MQ=255)
                                                            ccGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGa                                                                                      <  1:148522/151‑1 (MQ=255)
                                                                  tcGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAac                                                                                 >  2:1034979/1‑150 (MQ=255)
                                                                   cgcgTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAacca                                                                               <  4:600031/151‑1 (MQ=255)
                                                                   cgcgTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAacc                                                                                <  4:611954/150‑1 (MQ=255)
                                                                   cgcgTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAacc                                                                                <  4:115765/150‑1 (MQ=255)
                                                                    gcgTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAaccac                                                                              >  2:696507/1‑151 (MQ=255)
                                                                         tcGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAaccacaccac                                                                         >  2:1447774/1‑151 (MQ=255)
                                                                            atgatgGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCAt                                                                      <  2:1613654/151‑1 (MQ=255)
                                                                            atgatgGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCAt                                                                      <  4:1643046/151‑1 (MQ=255)
                                                                              gatgGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCAttt                                                                    <  1:94797/151‑1 (MQ=255)
                                                                               atgGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCAtttt                                                                   <  1:174218/151‑1 (MQ=255)
                                                                                  gTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTtctc                                                                >  2:1363907/1‑151 (MQ=255)
                                                                                     gTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCg                                                              >  4:1449835/1‑150 (MQ=255)
                                                                                       atctatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGt                                                           >  4:584218/1‑151 (MQ=255)
                                                                                         ctatcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTgg                                                         >  2:88027/1‑151 (MQ=255)
                                                                                           atcAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGt                                                        >  1:300840/1‑150 (MQ=255)
                                                                                              aaCGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATgc                                                    <  4:794610/151‑1 (MQ=255)
                                                                                                 gTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCt                                                 >  3:95236/1‑151 (MQ=255)
                                                                                                  tCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTg                                                >  2:1271543/1‑151 (MQ=255)
                                                                                                    cGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTgg                                               >  2:1021606/1‑150 (MQ=255)
                                                                                                    cGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGa                                              <  2:751710/151‑1 (MQ=255)
                                                                                                     gTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGAt                                             <  2:992147/151‑1 (MQ=255)
                                                                                                        tGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCt                                           <  2:1332813/150‑1 (MQ=255)
                                                                                                         ggCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTg                                          <  2:1512711/150‑1 (MQ=255)
                                                                                                          gCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTgg                                         >  2:833888/1‑150 (MQ=255)
                                                                                                            ggTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTg                                       >  1:163487/1‑150 (MQ=255)
                                                                                                               cAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGaaa                                    >  2:1248339/1‑150 (MQ=255)
                                                                                                                aGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGaaaaa                                  <  1:437505/151‑1 (MQ=255)
                                                                                                                aGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGaaaaa                                  <  3:288478/151‑1 (MQ=255)
                                                                                                                    gAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAAcc                                >  4:174122/1‑149 (MQ=255)
                                                                                                                    gAAAAACTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAg                              <  2:4880/151‑1 (MQ=255)
                                                                                                                        aaCTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATg                          >  2:228741/1‑151 (MQ=255)
                                                                                                                         aCTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATgg                         >  2:79143/1‑151 (MQ=255)
                                                                                                                          cTTCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGgc                        >  2:93658/1‑151 (MQ=255)
                                                                                                                            tCGGCGGCGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATg                          <  2:323902/147‑1 (MQ=255)
                                                                                                                              ggcggcGAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCgg                    <  4:705030/151‑1 (MQ=255)
                                                                                                                               gcggcgAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCgg                    >  4:1442258/1‑150 (MQ=255)
                                                                                                                                cggcgAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCggtg                  >  4:19980/1‑151 (MQ=255)
                                                                                                                                cggcgAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCggtg                  <  4:938320/151‑1 (MQ=255)
                                                                                                                                    gAGCAGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGtggtgg              >  4:917274/1‑151 (MQ=255)
                                                                                                                                        aGGCGGCACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTg          >  3:154316/1‑151 (MQ=255)
                                                                                                                                           cggcACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTAc       >  2:1641588/1‑151 (MQ=255)
                                                                                                                                           cggcACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTAc       <  3:266598/151‑1 (MQ=255)
                                                                                                                                            ggcACGCGCTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTAcc      >  4:1580893/1‑151 (MQ=255)
                                                                                                                                                cgcgcTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCa   <  2:1448025/150‑1 (MQ=255)
                                                                                                                                                cgcgcTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCAc  <  4:951332/151‑1 (MQ=255)
                                                                                                                                                cgcgcTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCAc  >  2:1144106/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACACGCTTTATCAGCAGAACCTGAAAAACCACACCACCATTTTCTCCGAGTGGTATGCGCTGGATCTGGTGAAAAACCAGGATGGCGCGGTGGTGGGTTGTACCGCAC  >  NC_000913/756159‑756453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: