Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 54,593 A→G V37A (GTG→GCG)  surA ← peptidyl‑prolyl cis‑trans isomerase (PPIase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091354,5930AG100.0% 401.3 / NA 110V37A (GTG→GCG) surApeptidyl‑prolyl cis‑trans isomerase (PPIase)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (58/52);  total (58/52)

Only 100 of 112 total aligned reads displayed.

TTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATT  >  NC_000913/54446‑54736
                                                                                                                                                   |                                                                                                                                               
ttCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGcgcg                                                                                                                                               >  2:1185771/1‑150 (MQ=255)
ttCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGcgcg                                                                                                                                               <  2:859808/150‑1 (MQ=255)
   tGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGc                                                                                                                                           >  2:1084693/1‑151 (MQ=255)
    gCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGcc                                                                                                                                          >  2:586175/1‑151 (MQ=255)
    gCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGcc                                                                                                                                          >  1:97485/1‑151 (MQ=255)
    gCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGca                                                                                                                                          <  4:1403594/151‑2 (MQ=255)
     ccccATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCg                                                                                                                                         >  3:319568/1‑151 (MQ=255)
      cccATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGt                                                                                                                                        <  2:1329157/151‑1 (MQ=255)
      cccATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGt                                                                                                                                        <  2:1391159/151‑1 (MQ=255)
       ccATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGtt                                                                                                                                       >  2:1490377/1‑151 (MQ=255)
            tGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTg                                                                                                                                   >  1:236611/1‑150 (MQ=255)
             gCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTg                                                                                                                                   <  3:362103/149‑1 (MQ=255)
              cAGGATGATTTGATCCATGATCAAACGTTCCATGCTTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTgacg                                                                                                                                >  4:1367590/1‑151 (MQ=255)
                    gatTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCt                                                                                                                          <  1:209033/151‑1 (MQ=255)
                    gatTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCt                                                                                                                          >  4:1661678/1‑151 (MQ=255)
                     atTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTg                                                                                                                         >  4:599415/1‑151 (MQ=255)
                        tGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCg                                                                                                                       >  2:969234/1‑150 (MQ=255)
                        tGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCg                                                                                                                       >  4:172761/1‑150 (MQ=255)
                         gATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGAc                                                                                                                     >  1:15917/1‑151 (MQ=255)
                          aTCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACt                                                                                                                    >  2:361709/1‑151 (MQ=255)
                          aTCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACt                                                                                                                    <  4:274244/151‑1 (MQ=255)
                           tCCATGATCAACCGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACtt                                                                                                                   >  3:13127/1‑151 (MQ=255)
                            ccATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACttt                                                                                                                  >  2:837621/1‑151 (MQ=255)
                            ccATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACtt                                                                                                                   <  3:141525/150‑1 (MQ=255)
                             cATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTa                                                                                                                 <  4:490343/151‑1 (MQ=255)
                                gATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCg                                                                                                              <  4:857069/151‑1 (MQ=255)
                                 aTCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGa                                                                                                             <  2:1226577/151‑1 (MQ=255)
                                  tCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGAc                                                                                                            >  4:1133672/1‑151 (MQ=255)
                                      aCGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTAcc                                                                                                        >  2:63245/1‑151 (MQ=255)
                                      aCGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTAcc                                                                                                        <  4:124335/151‑1 (MQ=255)
                                          tCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTggg                                                                                                    >  4:499379/1‑151 (MQ=255)
                                          tCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTgg                                                                                                     <  4:1561742/150‑1 (MQ=255)
                                          tCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTgg                                                                                                     <  2:845048/150‑1 (MQ=255)
                                            cATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTggggg                                                                                                  <  4:1411650/151‑1 (MQ=255)
                                                aTTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGc                                                                                                >  3:465479/1‑149 (MQ=255)
                                                  ttGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGc                                                                                             <  1:451369/150‑1 (MQ=255)
                                                  ttGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGc                                                                                             <  2:1410052/150‑1 (MQ=255)
                                                    ggtggCGCAGCGTCGCGTCATCAGGACGTTGGTGCCTTGCCTGAGCAGAGCTCAGTTTTACCTACTGCATTAATCCATCAACGTCGTTTTCCAGCGCGACGCCGCTATTGACGACGGGTGCGACTTTATCGACTACCCGGGGGGCACCGa                                                                                           >  4:1088445/1‑150 (MQ=255)
                                                     gtggCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGaaa                                                                                         <  4:670625/151‑1 (MQ=255)
                                                           cAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGt                                                                                    >  4:1655172/1‑150 (MQ=255)
                                                           cAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGt                                                                                    >  1:151354/1‑150 (MQ=255)
                                                           cAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTa                                                                                   <  4:31819/151‑1 (MQ=255)
                                                            aGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTAt                                                                                  >  3:136925/1‑151 (MQ=255)
                                                              cgtcgcgtcATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTc                                                                                <  2:858652/151‑1 (MQ=255)
                                                               gtcgcgtcATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTcg                                                                               <  4:1390287/151‑1 (MQ=255)
                                                                tcgcgtcATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTcgc                                                                              >  3:295218/1‑151 (MQ=255)
                                                                 cgcgtcATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTcgcg                                                                             <  2:1035991/151‑1 (MQ=255)
                                                                   cgtcATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGAt                                                                           >  4:433038/1‑151 (MQ=255)
                                                                    gtcATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCGGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGAt                                                                           >  4:1272476/1‑150 (MQ=255)
                                                                        tcaGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATgg                                                                      >  2:1221044/1‑151 (MQ=255)
                                                                                 tgctgcCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTCCCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCg                                                              <  4:1331136/150‑1 (MQ=255)
                                                                                 tgctgcCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCg                                                              >  3:130865/1‑150 (MQ=255)
                                                                                 tgctgcCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCg                                                              <  2:817249/150‑1 (MQ=255)
                                                                                    tgccttgcctGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCgaga                                                           >  4:1485474/1‑150 (MQ=255)
                                                                                    tgccttgcctGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCgaga                                                           >  2:1269238/1‑150 (MQ=255)
                                                                                    tgccttgcctGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCgaga                                                           >  4:39890/1‑150 (MQ=255)
                                                                                       cttgcctGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAagc                                                        >  2:503608/1‑150 (MQ=255)
                                                                                         tgcctGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAagca                                                       <  1:8281/149‑1 (MQ=255)
                                                                                          gcctGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAagcagc                                                     <  3:151498/150‑1 (MQ=255)
                                                                                           cctGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCACGATCATGGCGATACCGAGAAGCAGCGt                                                   <  4:25780/151‑1 (MQ=255)
                                                                                              gAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGtttt                                                >  4:671426/1‑151 (MQ=255)
                                                                                               agcagcGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTc                                               >  4:916921/1‑151 (MQ=255)
                                                                                                gcagcGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTcc                                              >  2:1100637/1‑151 (MQ=255)
                                                                                                  agcGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAg                                            <  4:1560869/151‑1 (MQ=255)
                                                                                                   gcGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGt                                           >  2:109379/1‑151 (MQ=255)
                                                                                                    cGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGtt                                          <  4:329505/151‑1 (MQ=255)
                                                                                                    cGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGtt                                          <  2:1476124/151‑1 (MQ=255)
                                                                                                     gTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGttc                                         <  4:1353997/151‑1 (MQ=255)
                                                                                                       tCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGttct                                        <  2:1126594/150‑1 (MQ=255)
                                                                                                        cAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGGAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGttctt                                       <  3:383527/150‑1 (MQ=255)
                                                                                                        cAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGttcttc                                      <  3:236626/151‑1 (MQ=255)
                                                                                                        cAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGttctta                                      <  1:465579/151‑2 (MQ=255)
                                                                                                         aGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCa                                     <  1:183658/151‑1 (MQ=255)
                                                                                                          gTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCAt                                    >  2:1211271/1‑151 (MQ=255)
                                                                                                            tttACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATAc                                  >  2:928300/1‑151 (MQ=255)
                                                                                                              tACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACtt                                >  4:615052/1‑151 (MQ=255)
                                                                                                                 cGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACttttt                             >  3:363107/1‑151 (MQ=255)
                                                                                                                 cGACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACttttt                             <  4:174308/151‑1 (MQ=255)
                                                                                                                  gACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTc                            <  4:497329/151‑1 (MQ=255)
                                                                                                                  gACTGCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTc                            >  3:242694/1‑151 (MQ=255)
                                                                                                                      gCATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCAtt                        <  4:970494/151‑1 (MQ=255)
                                                                                                                       cATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCAttt                       >  1:151117/1‑151 (MQ=255)
                                                                                                                       cATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCAttt                       >  2:829246/1‑151 (MQ=255)
                                                                                                                       cATTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCAttt                       >  4:1002218/1‑151 (MQ=255)
                                                                                                                        aTTAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTc                      <  4:586985/151‑1 (MQ=255)
                                                                                                                         ttAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTc                      >  2:467813/1‑150 (MQ=255)
                                                                                                                          tAATCCATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCa                     <  4:894366/150‑1 (MQ=255)
                                                                                                                              ccATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTa                 <  3:391475/150‑1 (MQ=255)
                                                                                                                              ccATCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTa                 >  2:714885/1‑150 (MQ=255)
                                                                                                                                 tCAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCg             >  4:1450528/1‑151 (MQ=255)
                                                                                                                                  cAACGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGc            <  4:1187266/151‑1 (MQ=255)
                                                                                                                                     cGTCGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACt         <  2:598594/151‑1 (MQ=255)
                                                                                                                                        cGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGc       <  4:1492245/150‑1 (MQ=255)
                                                                                                                                        cGCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCg      <  1:393836/151‑1 (MQ=255)
                                                                                                                                         gCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCgg     >  4:493760/1‑151 (MQ=255)
                                                                                                                                         gCTTTCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCgg     <  4:327146/151‑1 (MQ=255)
                                                                                                                                           tttCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGAt   >  3:256152/1‑151 (MQ=255)
                                                                                                                                           tttCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGAt   >  2:1598950/1‑151 (MQ=255)
                                                                                                                                            ttCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGAt   <  1:423819/150‑1 (MQ=255)
                                                                                                                                             tCCAGCGCGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGAtt  <  4:838724/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TTCTGCCCCATCTGCAGGATGATTTGATCCATGATCAAACGTTCCATGATTTGGTGGCGCAGCGTCGCGTCATCAGGAAGTTGCTGCCTTGCCTGAGCAGCGTTCAGTTTTACCGACTGCATTAATCCATCAACGTCGCTTTCCAGCACGACGCCGTTATTGACGACGGCTGCGACTTTATCGACTACCTGGGGGGCAGCGAAACTGGTATTCGCGATCATGGCGATACCGAGAAGCAGCGTTTTCCAGTTCTTCATACTTTTTCCATTTCAATTAACCGCACTGCGGATT  >  NC_000913/54446‑54736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑