Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 976,248 A→G E209E (GAA→GAG mukE → chromosome condensin MukBEF, MukE localization factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913976,2480AG99.3% 529.0 / ‑4.1 140E209E (GAA→GAGmukEchromosome condensin MukBEF, MukE localization factor
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (57/82);  total (58/82)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

Only 100 of 140 total aligned reads displayed.

ACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGGAACGGCTTTTTTGCCCGAACTTTTG  >  NC_000913/976100‑976395
                                                                                                                                                    |                                                                                                                                                   
aCCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGc                                                                                                                                                   >  3:429143/1‑151 (MQ=255)
 ccGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGc                                                                                                                                                   >  2:540602/1‑150 (MQ=255)
 ccGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCa                                                                                                                                                  <  3:199795/151‑1 (MQ=255)
  cGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCa                                                                                                                                                  >  4:1195091/1‑150 (MQ=255)
   gTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAAt                                                                                                                                                <  2:408953/151‑1 (MQ=255)
   gTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAAt                                                                                                                                                <  1:95723/151‑1 (MQ=255)
       gCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGcc                                                                                                                                             <  4:938298/150‑1 (MQ=255)
       gCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCg                                                                                                                                            <  2:487687/151‑1 (MQ=255)
        cgtcgtTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCg                                                                                                                                            >  3:279245/1‑150 (MQ=255)
         gtcgtTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGAt                                                                                                                                          <  4:283513/151‑1 (MQ=255)
               tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGaaa                                                                                                                                     <  2:780554/150‑1 (MQ=255)
                aGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGaaaa                                                                                                                                    <  2:43389/150‑1 (MQ=255)
                   cATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAatcat                                                                                                                                <  4:481124/151‑1 (MQ=255)
                   cATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAat                                                                                                                                   >  2:159249/1‑148 (MQ=255)
                      ggTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTg                                                                                                                             <  2:641298/151‑1 (MQ=255)
                       gtgtGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGc                                                                                                                            >  4:513271/1‑151 (MQ=255)
                         gtgGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCa                                                                                                                           <  4:643263/150‑1 (MQ=255)
                           ggTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACt                                                                                                                        >  3:361117/1‑151 (MQ=255)
                            gTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTc                                                                                                                       >  2:1356268/1‑151 (MQ=255)
                              ttATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCa                                                                                                                      <  2:437920/150‑1 (MQ=255)
                                aTGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACg                                                                                                                   <  4:1354548/151‑1 (MQ=255)
                                aTGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACg                                                                                                                   >  4:506966/1‑151 (MQ=255)
                                 tGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACg                                                                                                                   >  2:1079694/1‑150 (MQ=255)
                                  gggCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGAt                                                                                                                 <  3:473244/151‑1 (MQ=255)
                                   ggCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATg                                                                                                                >  4:213167/1‑151 (MQ=255)
                                     ccACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGaa                                                                                                              <  1:248820/151‑1 (MQ=255)
                                      cACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGaaa                                                                                                             <  2:684572/151‑1 (MQ=255)
                                      cACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGaaa                                                                                                             <  2:346232/151‑1 (MQ=255)
                                       aCGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAAc                                                                                                            >  2:742337/1‑151 (MQ=255)
                                        cGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAAcc                                                                                                           <  3:50524/151‑1 (MQ=255)
                                        cGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAAcc                                                                                                           <  1:49680/151‑1 (MQ=255)
                                         gACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCg                                                                                                          >  2:1225163/1‑151 (MQ=255)
                                            agcagcAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGaa                                                                                                        <  4:1124202/150‑1 (MQ=255)
                                               agcaAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAag                                                                                                       >  4:817931/1‑148 (MQ=255)
                                               agcaAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAgaga                                                                                                    <  4:1497394/151‑1 (MQ=255)
                                                gcaAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGaa                                                                                                   <  2:888865/151‑1 (MQ=255)
                                                   aGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCa                                                                                                <  2:867020/151‑1 (MQ=255)
                                                     tttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGc                                                                                              >  2:719800/1‑151 (MQ=255)
                                                      ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGcc                                                                                             >  2:1338408/1‑151 (MQ=255)
                                                      ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGcc                                                                                             >  3:100123/1‑151 (MQ=255)
                                                        cGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAg                                                                                           <  2:195973/151‑1 (MQ=255)
                                                        cGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAg                                                                                           <  4:919701/151‑1 (MQ=255)
                                                          cATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGAt                                                                                         <  3:173877/151‑1 (MQ=255)
                                                           aTTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGAt                                                                                         >  1:277287/1‑150 (MQ=255)
                                                           aTTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATa                                                                                        <  4:527224/151‑1 (MQ=255)
                                                             tACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAg                                                                                       <  2:1338893/150‑1 (MQ=255)
                                                             tACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGc                                                                                      >  1:158202/1‑151 (MQ=255)
                                                              aCCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCg                                                                                     <  1:154957/151‑1 (MQ=255)
                                                              aCCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCg                                                                                     >  2:186712/1‑151 (MQ=255)
                                                               ccGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCgg                                                                                    <  2:1246579/151‑1 (MQ=255)
                                                               ccGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCgg                                                                                    >  4:1547780/1‑151 (MQ=255)
                                                                cGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGga                                                                                   <  4:1174982/151‑1 (MQ=255)
                                                                 gAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCgg                                                                                    >  2:797435/1‑149 (MQ=255)
                                                                  aaTCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCgg                                                                                    >  4:922959/1‑148 (MQ=255)
                                                                     cGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGga                                                                              <  4:297425/151‑1 (MQ=255)
                                                                      ggTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACATCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGgaa                                                                             <  2:1146606/151‑1 (MQ=255)
                                                                         gtTCCGCTTCGGAGCCGATGTGCGTGCTGGTGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGgaaga                                                                           <  4:126338/150‑1 (MQ=255)
                                                                            ccGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGaataa                                                                       <  4:1425191/151‑1 (MQ=255)
                                                                               cTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATg                                                                     >  2:1525619/1‑150 (MQ=255)
                                                                                 tCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGa                                                                    <  1:8656/149‑1 (MQ=255)
                                                                                  cGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGAtt                                                                  <  2:1164927/150‑1 (MQ=255)
                                                                                   ggAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTg                                                                 >  3:62088/1‑150 (MQ=255)
                                                                                   ggAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGa                                                                <  4:1634023/151‑1 (MQ=255)
                                                                                      gCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAAcg                                                             >  1:207529/1‑151 (MQ=255)
                                                                                       ccGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAAcgc                                                            >  4:3265/1‑151 (MQ=255)
                                                                                       ccGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAAcgc                                                            <  1:279229/151‑1 (MQ=255)
                                                                                         gATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAAcgcc                                                           <  4:1621446/150‑2 (MQ=255)
                                                                                            gtgcgtgcTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTa                                                        <  2:1511499/150‑1 (MQ=255)
                                                                                                gtgcTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAAttt                                                   >  4:1178695/1‑151 (MQ=255)
                                                                                                  gcTGGCGACGGTCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTc                                                  <  3:390452/150‑1 (MQ=255)
                                                                                                  gcTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTc                                                  <  1:100109/150‑1 (MQ=255)
                                                                                                  gcTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTc                                                  <  1:356523/150‑1 (MQ=255)
                                                                                                  gcTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCg                                                 <  2:952911/151‑1 (MQ=255)
                                                                                                     ggCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTc                                              >  4:653673/1‑151 (MQ=255)
                                                                                                       cgacgaTCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCAc                                            <  2:1466098/151‑1 (MQ=255)
                                                                                                         acgaTCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTg                                          <  4:937505/151‑1 (MQ=255)
                                                                                                          cgaTCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTg                                          >  4:895955/1‑150 (MQ=255)
                                                                                                                 cGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGAt                                  >  1:316300/1‑151 (MQ=255)
                                                                                                                  gTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGAtt                                 >  4:212441/1‑151 (MQ=255)
                                                                                                                  gTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGAtt                                 <  1:388535/151‑1 (MQ=255)
                                                                                                                   tGAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGAtt                                 >  4:1129166/1‑150 (MQ=255)
                                                                                                                    gAAGCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTa                                <  4:1275882/150‑1 (MQ=255)
                                                                                                                       gCACAGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTg                            <  3:474353/151‑1 (MQ=255)
                                                                                                                        cacaGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTgg                           <  2:20902/151‑1 (MQ=255)
                                                                                                                         acaGCGTCGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGGa                          <  4:1371703/151‑1 (MQ=255)
                                                                                                                                cGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGGAACGGCtt                   <  2:1177214/151‑1 (MQ=255)
                                                                                                                                cGCCTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGGAACGGCt                    <  2:3260/150‑1 (MQ=255)
                                                                                                                                  ccTGATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGGAACGGCtttt                 <  2:508570/151‑1 (MQ=255)
                                                                                                                                     gATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGGAACGGCtttttt               <  4:1357826/150‑1 (MQ=255)
                                                                                                                                     gATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGGAACGGCTTTTTTg              <  4:1525757/151‑1 (MQ=255)
                                                                                                                                     gATTCGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGGAACGGCTTTTTTg              >  2:17305/1‑151 (MQ=255)
                                                                                                                                         cGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGGAACGGCTTTTTTGcca           <  2:595481/150‑2 (MQ=255)
                                                                                                                                         cGTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGGAACGGCTTTTTTGCCCg          <  4:1418202/151‑1 (MQ=255)
                                                                                                                                          gTGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGGAACGGCTTTTTTGCCCg          >  3:70956/1‑150 (MQ=255)
                                                                                                                                           tGATGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGGAACGGCTTTTTTGCCCGa         >  4:421777/1‑150 (MQ=255)
                                                                                                                                              tGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGGAACGGCTTTTTTGCCCGAACtt     >  4:606874/1‑151 (MQ=255)
                                                                                                                                              tGGCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGGAACGGCTTTTTTGCCCGAACtt     >  2:1502120/1‑151 (MQ=255)
                                                                                                                                               ggCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGGACCGGCTTTTTTGCCCGAACtt     <  2:1094245/150‑1 (MQ=255)
                                                                                                                                                gCGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGGAACGGCTTTTTTGCCCGAACtttt   <  2:1466327/151‑1 (MQ=255)
                                                                                                                                                 cGAGGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGGAACGGCTTTTTTGCCCGAACTTTTg  <  4:734810/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGGAACGGCTTTTTTGCCCGAACTTTTG  >  NC_000913/976100‑976395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: