Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	994,513	A→G	V107A (GTG→GCG)	ssuC ←	aliphatic sulfonate ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	994,513	0	A	G	100.0%	326.4 / NA	94	V107A (GTG→GCG)	ssuC	aliphatic sulfonate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/45);  total (49/45)

CTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCACGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCC  >  NC_000913/994365‑994657                                                                                                                                                     |                                                                                                                                                 ctcCACCAACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGcgcg                                                                                                                                                >  2:304499/1‑151 (MQ=255)   ccaccaACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGtt                                                                                                                                              <  2:941715/151‑1 (MQ=255)   ccaccaACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGtt                                                                                                                                              <  2:1230479/151‑1 (MQ=255)   ccaccaACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGtt                                                                                                                                              <  4:1251759/151‑1 (MQ=255)    caccaACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCCCCAGCGGGATCAGCGCCAGATGCGGCGCGTTg                                                                                                                                             <  1:66161/151‑1 (MQ=255)    caccaACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTg                                                                                                                                             >  1:212600/1‑151 (MQ=255)        aaCCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCa                                                                                                                                         <  4:1085179/151‑1 (MQ=255)          cccGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAAc                                                                                                                                       >  4:679946/1‑151 (MQ=255)          cccGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAAc                                                                                                                                       <  2:755196/151‑1 (MQ=255)             gcgATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATc                                                                                                                                    >  4:103178/1‑151 (MQ=255)              cgatcgatATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCt                                                                                                                                   <  2:68741/151‑1 (MQ=255)               gatcgatATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTg                                                                                                                                  >  2:111294/1‑151 (MQ=255)               gatcgatATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTg                                                                                                                                  <  4:1485495/151‑1 (MQ=255)                  cgatATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAAt                                                                                                                               >  4:633953/1‑151 (MQ=255)                   gatATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAAtt                                                                                                                              <  4:1461002/151‑1 (MQ=255)                   gatATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAAtt                                                                                                                              <  2:227621/151‑1 (MQ=255)                      atTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGa                                                                                                                            <  4:1629137/150‑1 (MQ=255)                      atTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAg                                                                                                                           <  4:262731/151‑1 (MQ=255)                       ttACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAgg                                                                                                                          >  1:200615/1‑151 (MQ=255)                       ttACGGATGCCATGCCAGGGGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAgg                                                                                                                          >  2:602378/1‑151 (MQ=255)                           ggATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATc                                                                                                                      >  4:456677/1‑151 (MQ=255)                           ggATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATc                                                                                                                      >  2:930767/1‑151 (MQ=255)                           ggATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATc                                                                                                                      >  2:916154/1‑151 (MQ=255)                             atgccatgccAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCca                                                                                                                    <  2:1135715/151‑1 (MQ=255)                               gccatgccaGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCcag                                                                                                                   <  4:1057014/150‑1 (MQ=255)                               gccatgccaGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGAGCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCcag                                                                                                                   <  3:282596/150‑1 (MQ=255)                                ccatgccaGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCcagca                                                                                                                 <  2:810235/151‑1 (MQ=255)                                  atgccaGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCagcagc                                                                                                               >  4:491921/1‑151 (MQ=255)                                    gccaGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGcc                                                                                                              <  4:1149757/150‑1 (MQ=255)                                    gccaGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCg                                                                                                             <  2:422281/151‑1 (MQ=255)                                       aGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTtc                                                                                                          >  4:1214432/1‑151 (MQ=255)                                           gtTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTccc                                                                                                      >  4:1004899/1‑151 (MQ=255)                                                tataAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCg                                                                                                 >  1:427737/1‑151 (MQ=255)                                                  taAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCg                                                                                                 >  2:686875/1‑149 (MQ=255)                                                      tGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGAt                                                                                             >  4:1141481/1‑149 (MQ=255)                                                        gggAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGcc                                                                                         >  4:223023/1‑151 (MQ=255)                                                         ggAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGccc                                                                                        >  2:881965/1‑151 (MQ=255)                                                          gAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCg                                                                                       >  4:575433/1‑151 (MQ=255)                                                           aaTAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGc                                                                                      <  4:407417/151‑1 (MQ=255)                                                           aaTAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGc                                                                                      <  4:286838/151‑1 (MQ=255)                                                            aTAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCt                                                                                     <  4:1292427/151‑1 (MQ=255)                                                            aTAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCt                                                                                     >  4:839437/1‑151 (MQ=255)                                                             tAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCt                                                                                     >  4:1454748/1‑150 (MQ=255)                                                               gCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAAt                                                                                  <  4:1355732/151‑1 (MQ=255)                                                                  tACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATc                                                                                 >  4:287066/1‑149 (MQ=255)                                                                   aCCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGt                                                                              >  4:978826/1‑151 (MQ=255)                                                                     cGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTcc                                                                            >  2:1095507/1‑151 (MQ=255)                                                                     cGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTcc                                                                            >  2:1610549/1‑151 (MQ=255)                                                                      gAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTccc                                                                           >  4:1611377/1‑151 (MQ=255)                                                                           ccaccaGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAAt                                                                      >  2:477279/1‑151 (MQ=255)                                                                            caccaGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATc                                                                     >  2:776113/1‑151 (MQ=255)                                                                             accaGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCa                                                                    <  3:188748/151‑1 (MQ=255)                                                                              ccaGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCaa                                                                   <  1:383732/151‑1 (MQ=255)                                                                                aGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAAcc                                                                 >  2:1631843/1‑151 (MQ=255)                                                                                  aaaGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAAacc                                                                >  4:1336627/1‑150 (MQ=255)                                                                                   aaGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCa                                                              <  2:177592/151‑1 (MQ=255)                                                                                    aGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAg                                                             <  3:476339/151‑1 (MQ=255)                                                                                    aGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAg                                                             <  2:243661/151‑1 (MQ=255)                                                                                    aGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAg                                                             <  3:309652/151‑1 (MQ=255)                                                                                     gATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGc                                                            >  2:1447511/1‑151 (MQ=255)                                                                                      aTTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCg                                                           <  4:1008171/151‑1 (MQ=255)                                                                                        ttttGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGa                                                          <  4:254198/150‑1 (MQ=255)                                                                                           tGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATcc                                                       <  2:296277/150‑1 (MQ=255)                                                                                           tGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCg                                                      >  2:1505112/1‑151 (MQ=255)                                                                                            gCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATcca                                                      <  2:1468511/150‑2 (MQ=255)                                                                                              ggATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCa                                                   <  4:1621289/151‑1 (MQ=255)                                                                                              ggATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATACAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCa                                                   <  4:1223318/151‑1 (MQ=255)                                                                                               gATACATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACCTCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCa                                                   >  1:204357/1‑150 (MQ=255)                                                                                                   catcaATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTg                                               <  4:1351548/150‑1 (MQ=255)                                                                                                   catcaATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTg                                               >  4:1043555/1‑150 (MQ=255)                                                                                                    atcaATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGa                                              <  4:281986/150‑1 (MQ=255)                                                                                                    atcaATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGa                                              >  4:534278/1‑150 (MQ=255)                                                                                                              aaCCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATc                                    >  4:808419/1‑150 (MQ=255)                                                                                                                ccACAAAATCGCCAGCGGGATCAGCGCCAGATGCAGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAgc                                 <  4:410735/151‑1 (MQ=255)                                                                                                                ccACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAgc                                 <  4:917331/151‑1 (MQ=255)                                                                                                                 cacaAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAgcg                                <  4:1511786/151‑1 (MQ=255)                                                                                                                  acaAAATCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAgcgc                               >  4:969067/1‑151 (MQ=255)                                                                                                                      aaTCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGcgcccgc                           >  4:1087900/1‑151 (MQ=255)                                                                                                                       aTCACCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGcgcccgcc                          <  2:491231/151‑1 (MQ=255)                                                                                                                          aCCAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAgg                       >  3:328658/1‑151 (MQ=255)                                                                                                                            cAGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAgg                       >  3:205849/1‑149 (MQ=255)                                                                                                                             aGCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGc                    >  3:316945/1‑151 (MQ=255)                                                                                                                              gCGGGATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCt                   <  1:35179/151‑1 (MQ=255)                                                                                                                                gggATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTg                  >  2:322762/1‑150 (MQ=255)                                                                                                                                gggATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGa                 <  2:1198415/151‑1 (MQ=255)                                                                                                                                 ggATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGAt                <  4:227584/151‑1 (MQ=255)                                                                                                                                  gATCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATc               >  4:176284/1‑151 (MQ=255)                                                                                                                                   aTCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCg              <  4:450413/151‑1 (MQ=255)                                                                                                                                   aTCAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCg              >  2:1180007/1‑151 (MQ=255)                                                                                                                                     cAGCGCCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGcc            >  4:311173/1‑151 (MQ=255)                                                                                                                                     cAGCGCCAGATCCGCGGCGTGGGGCAAAATGTGGATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGcc            >  2:826899/1‑151 (MQ=255)                                                                                                                                       gcgcCAGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGcc            >  3:125179/1‑149 (MQ=255)                                                                                                                                            aGATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGAtgct     >  4:1384407/1‑151 (MQ=255)                                                                                                                                             gATGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGAtgctg    <  4:563814/151‑1 (MQ=255)                                                                                                                                               tGCGGCGCGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGcc  >  2:910732/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CTCCACCAACCCGCGATCGATATTACGGATGCCATGCCAGGTGTTGATATAAATGGGGAATAGCGTACCGAGCGCCACCAGAAAGATTTTTGCGGATTCATCAATGCCAAACCACAAAATCACCAGCGGGATCAGCGCCAGATGCGGCACGTTGCGCAACATCTGAATTGAGGTATCCAGCAGCCGTTCTCCCCAGCGAGATAGCCCGCTAATCAGTCCCAAAATCAACCCCAGCGATCCGCCAATTGAAAAGCCAATCAGCGCCCGCCAGGAGCTGATCGCCAGATGCTGCC  >  NC_000913/994365‑994657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑