Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,061,552 G→A R766Q (CGA→CAA)  torA → trimethylamine N‑oxide (TMAO) reductase I, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,061,5520GA99.2% 461.2 / ‑5.3 118R766Q (CGA→CAA) torAtrimethylamine N‑oxide (TMAO) reductase I, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (57/60);  total (58/60)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 118 total aligned reads displayed.

AGCGGGTAAAGAGCCAGTATTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACGAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAA  >  NC_000913/1061406‑1061697
                                                                                                                                                  |                                                                                                                                                 
aGCGGGTAAAGAGCCAGTATTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAAtt                                                                                                                                               <  2:41359/151‑1 (MQ=255)
  tgggTAAAGAGCCAGTATTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTc                                                                                                                                              >  2:1592494/2‑150 (MQ=255)
    tgTAAAGAGCCAGTATTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACg                                                                                                                                           >  1:51389/2‑151 (MQ=255)
      tAAAGAGCCAGTATTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGa                                                                                                                                          >  3:274808/1‑150 (MQ=255)
       aaaGAGCCAGTATTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGaa                                                                                                                                         <  1:277323/150‑1 (MQ=255)
       aaaGAGCCAGTATTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGa                                                                                                                                          >  2:1067211/1‑149 (MQ=255)
        aaGAGCCAGTATTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAgg                                                                                                                                       >  2:734061/1‑151 (MQ=255)
        aaGAGCCAGTATTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAgg                                                                                                                                       >  2:1512638/1‑151 (MQ=255)
         agagCCAGTATTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAggg                                                                                                                                      <  2:709009/151‑1 (MQ=255)
         agagCCAGTATTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGAAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAggg                                                                                                                                      >  3:123489/1‑151 (MQ=255)
           agCCAGTATTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGc                                                                                                                                    <  4:724204/151‑1 (MQ=255)
             ccAGTATTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCAt                                                                                                                                  <  1:71450/151‑1 (MQ=255)
              cAGTATTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATg                                                                                                                                 >  2:159601/1‑151 (MQ=255)
               aGTATTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATgg                                                                                                                                <  4:923948/151‑1 (MQ=255)
               aGTATTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATgg                                                                                                                                <  2:1028907/151‑1 (MQ=255)
                gTATTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGt                                                                                                                               >  1:389996/1‑151 (MQ=255)
                  aTTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTa                                                                                                                              <  2:268571/150‑1 (MQ=255)
                    tCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTAct                                                                                                                            <  4:1510362/150‑2 (MQ=255)
                    tCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACg                                                                                                                            >  2:615618/1‑150 (MQ=255)
                    tCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACg                                                                                                                            >  2:74820/1‑150 (MQ=255)
                     cATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGa                                                                                                                           <  3:328546/150‑1 (MQ=255)
                     cATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGAt                                                                                                                          >  4:1665301/1‑151 (MQ=255)
                     cATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGAt                                                                                                                          >  4:1646858/1‑151 (MQ=255)
                      aTTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATc                                                                                                                         <  2:1146760/151‑1 (MQ=255)
                        tAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATcc                                                                                                                        >  1:354761/1‑150 (MQ=255)
                        tAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATcc                                                                                                                        <  2:979961/150‑1 (MQ=255)
                        tAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATcc                                                                                                                        <  4:506119/150‑1 (MQ=255)
                         aaCCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCa                                                                                                                       <  1:462123/150‑1 (MQ=255)
                            ccGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGAt                                                                                                                    >  2:1640817/1‑150 (MQ=255)
                              gCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATaa                                                                                                                  >  2:1276671/1‑150 (MQ=255)
                              gCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATaa                                                                                                                  >  4:279880/1‑150 (MQ=255)
                               cAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAg                                                                                                                <  4:622174/151‑1 (MQ=255)
                                  gATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAggcg                                                                                                             >  3:427935/1‑151 (MQ=255)
                                  gATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAggcg                                                                                                             <  4:625651/151‑1 (MQ=255)
                                   aTGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAggcgg                                                                                                            <  4:1544637/151‑1 (MQ=255)
                                     gCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGgcggcg                                                                                                          >  4:693606/1‑151 (MQ=255)
                                      ccAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGgcggcg                                                                                                          >  2:1524711/1‑150 (MQ=255)
                                      ccAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGa                                                                                                         <  3:21489/151‑1 (MQ=255)
                                       cAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAg                                                                                                        <  2:635989/151‑1 (MQ=255)
                                           gcgcgcgGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCt                                                                                                     >  2:786409/1‑150 (MQ=255)
                                           gcgcgcgGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTg                                                                                                    <  2:1646336/151‑1 (MQ=255)
                                             gcgcgGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTgg                                                                                                   >  4:407709/1‑150 (MQ=255)
                                             gcgcgGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGt                                                                                                  <  2:1064467/151‑1 (MQ=255)
                                             gcgcgGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGt                                                                                                  <  2:1114591/151‑1 (MQ=255)
                                              cgcgGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTg                                                                                                 >  2:388258/1‑151 (MQ=255)
                                               gcgGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTgc                                                                                                <  2:239402/151‑1 (MQ=255)
                                                cgGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTgcg                                                                                               >  2:1014119/1‑151 (MQ=255)
                                                cgGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGGgcg                                                                                               >  3:87547/1‑151 (MQ=255)
                                                    aTTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCtgt                                                                                           <  4:1087084/151‑1 (MQ=255)
                                                     ttCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCtgtg                                                                                          >  4:891129/1‑151 (MQ=255)
                                                     ttCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCtgtg                                                                                          >  4:775808/1‑151 (MQ=255)
                                                      tCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCtgtg                                                                                          <  4:1270067/150‑1 (MQ=255)
                                                      tCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGc                                                                                         >  2:383146/1‑151 (MQ=255)
                                                       cGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGc                                                                                         >  4:669365/1‑150 (MQ=255)
                                                          aaCGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCaaa                                                                                      <  1:40770/150‑1 (MQ=255)
                                                          aaCGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCaaa                                                                                      <  1:221600/150‑1 (MQ=255)
                                                          aaCGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAAt                                                                                     <  4:105908/151‑1 (MQ=255)
                                                           aCGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAAt                                                                                     >  3:161643/1‑150 (MQ=255)
                                                              gTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACgg                                                                                 <  4:1350855/151‑1 (MQ=255)
                                                                   gtgGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAAcc                                                                            <  3:380620/151‑1 (MQ=255)
                                                                   gtgGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAAcc                                                                            <  1:294485/151‑1 (MQ=255)
                                                                      gTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCa                                                                         <  3:445831/151‑1 (MQ=255)
                                                                         cgcgTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACg                                                                      >  4:559860/1‑151 (MQ=255)
                                                                          gcgTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACGAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACgt                                                                     >  2:141891/1‑151 (MQ=255)
                                                                           cgTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACgtg                                                                    >  4:656037/1‑151 (MQ=255)
                                                                                  aaCGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGAcc                                                              <  2:1196113/150‑1 (MQ=255)
                                                                                    cGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGTGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATc                                                           <  4:1249670/151‑1 (MQ=255)
                                                                                    cGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATc                                                           >  2:1357401/1‑151 (MQ=255)
                                                                                        cgcgGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGAc                                                        <  4:1514529/150‑1 (MQ=255)
                                                                                          cgGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATc                                                     <  1:32118/151‑1 (MQ=255)
                                                                                          cgGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATc                                                     <  4:52693/151‑1 (MQ=255)
                                                                                            gTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTCTGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGGGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCgg                                                   >  1:459823/1‑151 (MQ=255)
                                                                                              cAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGt                                                  >  4:226821/1‑150 (MQ=255)
                                                                                               aGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTAc                                                >  2:11350/1‑151 (MQ=255)
                                                                                                ggTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTAc                                                >  4:1475421/1‑150 (MQ=255)
                                                                                                   gtTGGCAGGGGCAGTGGTTTCTGACAGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGTTCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCg                                            >  4:522638/1‑151 (MQ=255)
                                                                                                     tGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGc                                           >  3:266979/1‑150 (MQ=255)
                                                                                                            ggCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGgcg                                   <  4:357713/151‑1 (MQ=255)
                                                                                                            ggCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGgcg                                   <  4:72004/151‑1 (MQ=255)
                                                                                                              cAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGgcgc                                  >  4:847865/1‑150 (MQ=255)
                                                                                                                gTGGTTTCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAgg                               <  2:432540/151‑1 (MQ=255)
                                                                                                                      tCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGAcc                          >  2:935875/1‑150 (MQ=255)
                                                                                                                      tCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGAcc                          <  4:1192053/150‑1 (MQ=255)
                                                                                                                      tCTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGAcc                          <  2:115393/150‑1 (MQ=255)
                                                                                                                       cTGACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTAGATCGCAGCTGGCGCAGGCGAcc                          >  2:782863/1‑149 (MQ=255)
                                                                                                                         gACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCa                         <  4:1594458/148‑1 (MQ=255)
                                                                                                                         gACCGCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTg                      >  4:1406262/1‑151 (MQ=255)
                                                                                                                             gCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTgcgc                   >  4:670534/1‑150 (MQ=255)
                                                                                                                             gCTATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGca                  <  1:292833/151‑1 (MQ=255)
                                                                                                                               tATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGcac                 >  1:200299/1‑150 (MQ=255)
                                                                                                                               tATGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGcac                 >  4:528129/1‑150 (MQ=255)
                                                                                                                                aTGCACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCacac               >  2:1108954/1‑151 (MQ=255)
                                                                                                                                   cACCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTAc            <  4:898633/151‑1 (MQ=255)
                                                                                                                                    aCCCGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACg           <  2:559090/151‑1 (MQ=255)
                                                                                                                                     cccGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGc          <  4:491715/151‑1 (MQ=255)
                                                                                                                                       cGGCGTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCtg        <  2:889971/151‑1 (MQ=255)
                                                                                                                                           gTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCtggtgg    <  4:616452/151‑1 (MQ=255)
                                                                                                                                           gTGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCtggtgg    >  4:609987/1‑151 (MQ=255)
                                                                                                                                            tGGCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCtggtgg    >  2:40404/1‑150 (MQ=255)
                                                                                                                                             ggCACAAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGaa  <  4:1205093/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AGCGGGTAAAGAGCCAGTATTCATTAACCCGCAGGATGCCAGCGCGCGCGGTATTCGTAACGGTGATGTGGTACGCGTCTTTAACGCTCGCGGTCAGGTGTTGGCAGGGGCAGTGGTTTCTGACCGCTATGCACCCGGCGTGGCACGAATTCACGAAGGGGCATGGTACGATCCAGATAAAGGCGGCGAGCCTGGTGCGCTGTGCAAATACGGTAACCCCAACGTGTTGACCATCGACATCGGTACATCGCAGCTGGCGCAGGCGACCAGTGCGCACACTACGCTGGTGGAA  >  NC_000913/1061406‑1061697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: