Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 57,698 G→A R112H (CGC→CAC)  djlA → membrane‑anchored DnaK co‑chaperone, DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091357,6980GA100.0% 390.3 / NA 100R112H (CGC→CAC) djlAmembrane‑anchored DnaK co‑chaperone, DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (47/53);  total (47/53)

Only 100 of 103 total aligned reads displayed.

GGGCATTTAACCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCGCGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCTGTATGTCATTGCAGAAG  >  NC_000913/57550‑57841
                                                                                                                                                       |                                                                                                                                               
gggCATTTAACCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGcacg                                                                                                                                               <  2:1096432/151‑1 (MQ=255)
gggCATTTAACCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGcacg                                                                                                                                               <  2:81100/151‑1 (MQ=255)
gggCATTTAACCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGcacg                                                                                                                                               <  3:460491/151‑1 (MQ=255)
 ggCATTTAACCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGa                                                                                                                                              <  3:216732/151‑1 (MQ=255)
 ggCATTTAACCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGa                                                                                                                                              <  4:1668754/151‑1 (MQ=255)
   cATTTAACCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGaaa                                                                                                                                            <  4:32978/151‑1 (MQ=255)
    aTTTAACCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGaaaa                                                                                                                                           <  2:921470/151‑1 (MQ=255)
    aTTTAACCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGaaaa                                                                                                                                           <  2:520835/151‑1 (MQ=255)
    aTTTAACCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGaaa                                                                                                                                            >  2:1379077/1‑150 (MQ=255)
    aTTTAACCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGaaa                                                                                                                                            >  1:196522/1‑150 (MQ=255)
      ttAACCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGaaa                                                                                                                                            >  2:1622593/1‑148 (MQ=255)
        aaCCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGAt                                                                                                                                        <  4:1184324/150‑1 (MQ=255)
        aaCCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGAt                                                                                                                                        <  2:1044519/150‑1 (MQ=255)
        aaCCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGAt                                                                                                                                        <  1:378274/150‑1 (MQ=255)
         aCCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATgc                                                                                                                                      >  1:94734/1‑151 (MQ=255)
          ccAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATgcg                                                                                                                                     <  4:126499/151‑1 (MQ=255)
           cAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATgcgc                                                                                                                                    >  3:237801/1‑151 (MQ=255)
             aaTCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCa                                                                                                                                  <  4:257638/151‑1 (MQ=255)
              aTCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAg                                                                                                                                 <  4:413741/151‑1 (MQ=255)
                ccAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGtt                                                                                                                               <  4:509248/151‑1 (MQ=255)
                    aGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGc                                                                                                                           <  3:145423/151‑1 (MQ=255)
                    aGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGc                                                                                                                           <  3:9760/151‑1 (MQ=255)
                    aGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGc                                                                                                                           >  2:488619/1‑151 (MQ=255)
                       tCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAgt                                                                                                                        >  4:190842/1‑151 (MQ=255)
                          cgTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTc                                                                                                                     <  4:982449/151‑1 (MQ=255)
                          agTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTc                                                                                                                     >  4:736050/2‑151 (MQ=255)
                           gTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCt                                                                                                                    <  4:1368/151‑1 (MQ=255)
                              aCGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCt                                                                                                                 >  4:1015920/1‑151 (MQ=255)
                                ggAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCtt                                                                                                                <  1:108499/150‑1 (MQ=255)
                                 gAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTg                                                                                                              >  1:372849/1‑151 (MQ=255)
                                  aGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTgg                                                                                                             >  4:1542329/1‑151 (MQ=255)
                                        atatTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGttt                                                                                                       >  2:1519386/1‑151 (MQ=255)
                                        atatTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGttt                                                                                                       >  2:492755/1‑151 (MQ=255)
                                           ttCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTg                                                                                                     >  2:1322885/1‑150 (MQ=255)
                                             cATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGAc                                                                                                   <  1:237786/150‑1 (MQ=255)
                                             cATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACt                                                                                                  >  1:224408/1‑151 (MQ=255)
                                                atCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTaa                                                                                               <  1:110159/151‑1 (MQ=255)
                                                 tCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAAt                                                                                              >  2:1605413/1‑151 (MQ=255)
                                                  cGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAAtt                                                                                             >  3:370631/1‑151 (MQ=255)
                                                   gccagccaGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTc                                                                                            >  4:475228/1‑151 (MQ=255)
                                                     cagccagTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGt                                                                                          >  1:435411/1‑151 (MQ=255)
                                                      agccagTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTa                                                                                         <  4:1751/151‑1 (MQ=255)
                                                       gccagTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTAt                                                                                        <  1:137390/151‑1 (MQ=255)
                                                        ccagTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATg                                                                                       <  4:886533/151‑1 (MQ=255)
                                                          agTTGATGGACCGAATGAATCTTCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTAt                                                                                        >  2:1133753/1‑151 (MQ=255)
                                                          agTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGtt                                                                                     <  4:400820/151‑1 (MQ=255)
                                                           gTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGttt                                                                                    <  1:269978/151‑1 (MQ=255)
                                                              gATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTg                                                                                 >  3:7997/1‑151 (MQ=255)
                                                              gATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTg                                                                                 <  1:99611/151‑1 (MQ=255)
                                                                 ggACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTgg                                                                                >  4:1270376/1‑149 (MQ=255)
                                                                   aCCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTgg                                                                                >  1:242302/1‑147 (MQ=255)
                                                                     cGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATcc                                                                          >  2:920698/1‑151 (MQ=255)
                                                                       aatgaa‑‑‑tCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAg                                                                        <  1:2568/151‑1 (MQ=255)
                                                                          gaa‑‑‑tCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGAtt                                                                     >  2:98972/1‑151 (MQ=255)
                                                                          gaa‑‑‑tCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGAt                                                                      <  1:334058/150‑1 (MQ=255)
                                                                                   tCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAgg                                                                 <  4:1293744/149‑1 (MQ=255)
                                                                                     aTGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAggcggc                                                             >  3:164287/1‑151 (MQ=255)
                                                                                      tGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGgcggcg                                                            >  4:1394631/1‑151 (MQ=255)
                                                                                      tGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGgcggcg                                                            >  4:477985/1‑151 (MQ=255)
                                                                                         cgcTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGttt                                                         <  4:384598/151‑1 (MQ=255)
                                                                                          gcTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTg                                                        >  2:136145/1‑151 (MQ=255)
                                                                                            ttCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCt                                                      >  4:1545411/1‑151 (MQ=255)
                                                                                              cccGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTg                                                     >  2:395865/1‑150 (MQ=255)
                                                                                              cccGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGa                                                    <  2:706859/151‑1 (MQ=255)
                                                                                                cGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATg                                                  <  4:1130599/151‑1 (MQ=255)
                                                                                                      gcggcgCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCAc                                            <  2:944486/151‑1 (MQ=255)
                                                                                                       cggcgCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACt                                           <  1:144339/151‑1 (MQ=255)
                                                                                                         gcgcAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGc                                         >  4:1671861/1‑151 (MQ=255)
                                                                                                          cgcAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCa                                        <  2:469874/151‑1 (MQ=255)
                                                                                                          cgcAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCa                                        <  4:684369/151‑1 (MQ=255)
                                                                                                           gcAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCa                                        <  4:1130622/150‑1 (MQ=255)
                                                                                                           gcAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCAc                                       >  2:829351/1‑151 (MQ=255)
                                                                                                               aaTGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGa                                   <  3:121735/151‑1 (MQ=255)
                                                                                                                aTGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGaa                                  <  4:51498/151‑1 (MQ=255)
                                                                                                                  gCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAAt                                 <  3:146879/150‑1 (MQ=255)
                                                                                                                  gCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATg                                >  2:1157555/1‑151 (MQ=255)
                                                                                                                    gTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGa                               >  2:157555/1‑150 (MQ=255)
                                                                                                                      tCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACg                            >  2:278294/1‑151 (MQ=255)
                                                                                                                      tCCGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACg                            >  4:1541642/1‑151 (MQ=255)
                                                                                                                        cGGGTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACggg                          >  2:1646454/1‑151 (MQ=255)
                                                                                                                         gggTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGc                         <  4:104939/151‑1 (MQ=255)
                                                                                                                         gggTGGGAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGc                         >  2:1081089/1‑151 (MQ=255)
                                                                                                                             gggAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGt                      <  1:109449/150‑1 (MQ=255)
                                                                                                                              ggAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGc                    <  2:411868/151‑1 (MQ=255)
                                                                                                                               gAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCt                   <  2:438726/151‑1 (MQ=255)
                                                                                                                               gAAAATCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCt                   <  4:1607377/151‑1 (MQ=255)
                                                                                                                                 aaaTCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCTGt                 >  4:173185/1‑151 (MQ=255)
                                                                                                                                   aTCAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTTGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCTGTAt               <  2:338926/151‑1 (MQ=255)
                                                                                                                                     cAGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCTGTATg              <  2:902584/150‑1 (MQ=255)
                                                                                                                                      aGACAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCTGTATGTc            <  2:678706/151‑1 (MQ=255)
                                                                                                                                         cAATTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTTTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGGTCACTGCACCCGAATGAACGGGCGGTGCTGTATGTCAtt         <  4:104616/151‑1 (MQ=255)
                                                                                                                                          aaTTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCTGTATGTCATTg        >  1:427629/1‑151 (MQ=255)
                                                                                                                                           aTTACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCTGTATGTCATTGc       >  4:1307873/1‑151 (MQ=255)
                                                                                                                                            ttACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTACTGTATGTCATTGc       >  1:113276/1‑150 (MQ=255)
                                                                                                                                             tACCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCTGTATGTCATTGc       >  2:1111885/1‑149 (MQ=255)
                                                                                                                                              aCCCGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCTGTATGTCATTGc       >  4:517318/1‑148 (MQ=255)
                                                                                                                                               cccGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCTGTATGTCATTGCaga    >  2:999294/1‑150 (MQ=255)
                                                                                                                                                cctcTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCTGTATGTCATTGCagaag  <  1:96265/148‑1 (MQ=255)
                                                                                                                                                ccGCTGCACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCTGTATGTCATTGCaga    >  2:404770/1‑149 (MQ=255)
                                                                                                                                                ccGCTGAACGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCTGTATGTCATTGCagaag  <  4:578690/151‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                               
GGGCATTTAACCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCCAGCCAGTTGATGGACCGAATGAA‑‑‑TCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGACAATTACCCGCTGCGCGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGGAGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCTGTATGTCATTGCAGAAG  >  NC_000913/57550‑57841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑