Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,072,993	A→G	V340A (GTG→GCG)	rutA ←	pyrimidine oxygenase, FMN‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,072,993	0	A	G	100.0%	315.1 / NA	86	V340A (GTG→GCG)	rutA	pyrimidine oxygenase, FMN‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/45);  total (41/45)

GAGCGGTTAAGGTCGTCATCATGCCACCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCGGAGCGGGTATCTTTCTGACTTTGTTCGG  >  NC_000913/1072845‑1073140                                                                                                                                                     |                                                                                                                                                    gAGCGGTTAAGGTCGTCATCATGCCACCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGcgcg                                                                                                                                                   >  2:773997/1‑151 (MQ=255)   gCGGTTAAGGTCGTCATCATGCCACCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCt                                                                                                                                                 <  4:604840/151‑1 (MQ=255)   gCGGTTAAGGTCGTCATCATGCCACCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCt                                                                                                                                                 <  2:1655374/151‑1 (MQ=255)    cGGTTAAGGTCGTCATCATGCCACCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCtt                                                                                                                                                <  3:23288/151‑1 (MQ=255)     ggTTAAGGTCGTCATCATGCCACCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTg                                                                                                                                               >  2:1677230/1‑151 (MQ=255)     ggTTAAGGTCGTCATCATGCCACCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGAGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCtt                                                                                                                                                <  4:721503/150‑1 (MQ=255)            gtcgtcATCATGCCACCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTc                                                                                                                                        <  2:531810/151‑1 (MQ=255)             tcgtcATCATGCCACCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTc                                                                                                                                        >  2:1268004/1‑150 (MQ=255)               gtcATCATGCCACCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATc                                                                                                                                     >  3:3440/1‑151 (MQ=255)                 catcatGCCACCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCt                                                                                                                                    <  1:457246/150‑1 (MQ=255)                   tcatGCCACCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAAc                                                                                                                                 >  2:1452978/1‑151 (MQ=255)                      tGCCACCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATg                                                                                                                              >  4:446164/1‑151 (MQ=255)                          aCCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATgcgcg                                                                                                                          <  2:1104111/151‑1 (MQ=255)                          aCCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATgcgc                                                                                                                           >  1:239215/1‑150 (MQ=255)                           cctcctGAGTCAGCGCAGGTAGATCGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATgcgcgc                                                                                                                         >  3:267848/1‑151 (MQ=255)                             tcctGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGcgcgcg                                                                                                                        >  4:1265801/1‑150 (MQ=255)                              cctGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGcgcgcg                                                                                                                        <  4:1681008/149‑1 (MQ=255)                                  aGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTg                                                                                                                  >  1:434339/1‑151 (MQ=255)                                    tCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGc                                                                                                                >  2:75523/1‑151 (MQ=255)                                       gcgcAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTa                                                                                                             <  2:440533/151‑1 (MQ=255)                                        cgcAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTa                                                                                                             >  4:438005/1‑150 (MQ=255)                                        cgcAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTa                                                                                                             >  3:79599/1‑150 (MQ=255)                                         gcAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACGCTGGCGTaa                                                                                                            <  2:445529/150‑1 (MQ=255)                                         gcAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTa                                                                                                             >  2:1004396/1‑149 (MQ=255)                                         gcAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAg                                                                                                           <  2:214528/151‑1 (MQ=255)                                          cAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTa                                                                                                             >  4:222621/1‑148 (MQ=255)                                               aGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCg                                                                                                      >  2:763557/1‑150 (MQ=255)                                               aGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGa                                                                                                     <  4:259675/151‑1 (MQ=255)                                                   gggCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTa                                                                                                  <  3:434357/150‑1 (MQ=255)                                                   gggCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTa                                                                                                  <  4:630349/150‑1 (MQ=255)                                                    ggCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAAc                                                                                                <  4:1442355/151‑1 (MQ=255)                                                     gcgcGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACg                                                                                               <  4:1269596/151‑1 (MQ=255)                                                     gcgcGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACg                                                                                               <  2:388215/151‑1 (MQ=255)                                                       gcgGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTc                                                                                             >  4:641803/1‑151 (MQ=255)                                                         ggCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTccc                                                                                           <  4:1471726/151‑1 (MQ=255)                                                          gCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTcccc                                                                                          >  3:289058/1‑151 (MQ=255)                                                            aTTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCat                                                                                        <  4:5539/151‑1 (MQ=255)                                                             ttGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCat                                                                                        >  1:46986/1‑150 (MQ=255)                                                              tGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCatat                                                                                      >  1:46212/1‑151 (MQ=255)                                                                cATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTg                                                                                    >  1:208139/1‑151 (MQ=255)                                                                 aTCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTg                                                                                    >  2:1653575/1‑150 (MQ=255)                                                                   cAGTGGTTGACTGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATg                                                                                 <  2:1591706/151‑1 (MQ=255)                                                                     gTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGta                                                                               <  1:279940/151‑2 (MQ=255)                                                                        gTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTAc                                                                            <  4:1356593/151‑1 (MQ=255)                                                                          tGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTAccg                                                                          >  2:261462/1‑151 (MQ=255)                                                                           gAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTAccgc                                                                         <  4:471619/151‑1 (MQ=255)                                                                             aTGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTAccgccg                                                                       <  2:1375864/151‑1 (MQ=255)                                                                               gcgcTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGa                                                                      <  4:796182/150‑1 (MQ=255)                                                                               gcgcTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGa                                                                      <  3:379374/150‑1 (MQ=255)                                                                               gcgcTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGa                                                                      <  2:1010450/150‑1 (MQ=255)                                                                                cgcTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGa                                                                      >  4:616958/1‑149 (MQ=255)                                                                                  cTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTg                                                                  >  2:1174940/1‑151 (MQ=255)                                                                                   tCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTgg                                                                 >  4:698598/1‑151 (MQ=255)                                                                                     gCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGa                                                               <  2:1052450/151‑1 (MQ=255)                                                                                      ccGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGAt                                                              <  1:335080/151‑1 (MQ=255)                                                                                       cGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATc                                                             <  2:1138357/151‑1 (MQ=255)                                                                                         aaGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCgg                                                           >  2:1332169/1‑151 (MQ=255)                                                                                            gTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCa                                                        <  2:84996/151‑1 (MQ=255)                                                                                             tttCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCAt                                                       >  2:1067974/1‑151 (MQ=255)                                                                                              ttCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATc                                                      >  2:588674/1‑151 (MQ=255)                                                                                               tCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCt                                                     >  2:645969/1‑151 (MQ=255)                                                                                                  aTTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGAc                                                  >  2:437254/1‑151 (MQ=255)                                                                                                       cGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACg                                             <  2:7491/151‑1 (MQ=255)                                                                                                         aCAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGtt                                           <  1:310327/151‑1 (MQ=255)                                                                                                         aCAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGtt                                           >  4:1011742/1‑151 (MQ=255)                                                                                                         aCAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGtt                                           >  2:146709/1‑151 (MQ=255)                                                                                                          cAGAAAATCGTCGAAGGTTAACAGCCCGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTATCGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACTTTg                                          <  2:1615008/151‑1 (MQ=255)                                                                                                          cAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTg                                          >  4:987705/1‑151 (MQ=255)                                                                                                                aTCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTc                                     >  2:893843/1‑150 (MQ=255)                                                                                                                aTCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCa                                    <  3:396850/151‑1 (MQ=255)                                                                                                                   gtcgAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAg                                   <  2:58545/149‑1 (MQ=255)                                                                                                                   gtcgAAGGTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTa                                 <  2:193943/151‑1 (MQ=255)                                                                                                                         ggTTAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCGGAg                           <  2:360518/151‑1 (MQ=255)                                                                                                                            tAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCGGAGCgg                        >  4:830049/1‑151 (MQ=255)                                                                                                                            tAACAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCGGAGCg                         <  4:842145/150‑1 (MQ=255)                                                                                                                             aaCAGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCGGAGCggg                       <  4:1104208/151‑1 (MQ=255)                                                                                                                                aGCACGCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCGGAGCGGGTAt                    >  4:1599989/1‑151 (MQ=255)                                                                                                                                     gCCTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCGGAGCGGGTATCTTTc               >  2:317978/1‑151 (MQ=255)                                                                                                                                      ccTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCGGAGCGGGTATCTTTCt              <  4:1369805/151‑1 (MQ=255)                                                                                                                                      ccTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCGGAGCGGGTATCTTTCt              <  2:1655479/151‑1 (MQ=255)                                                                                                                                      ccTTCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCGGAGCGGGTATCTTTCt              >  4:142524/1‑151 (MQ=255)                                                                                                                                        ttCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCGGAGCGGGTATCTTTCTg             >  1:128095/1‑150 (MQ=255)                                                                                                                                         tCGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCGGAGCGGGTATCTTTCTGAc           >  2:61423/1‑151 (MQ=255)                                                                                                                                          cGGCACCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCGGAGCGGGTATCTTTCTGACt          >  4:581970/1‑151 (MQ=255)                                                                                                                                              aCCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCGGAGCGGGTATCTTTCTGACTTTGt      <  2:222263/151‑1 (MQ=255)                                                                                                                                              aCCAGGCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCGGAGCGGGTATCTTTCTGACTTTGt      <  3:279220/151‑1 (MQ=255)                                                                                                                                                  ggCGCGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCGGAGCGGGTATCTTTCTGACTTTGTTCgg  <  4:1679264/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    GAGCGGTTAAGGTCGTCATCATGCCACCTCCTGAGTCAGCGCAGGTAGATGGGCGCGGCATTGCATCAGTGGTTGAATGCGCTCGCCGAAGGTTTCGATTCCCGACAGAAAATCGTCGAAGGTTAACAGCACGCCTTCGGCACCAGGCACGCTTGCGACTTCATCTAACATGCGCGCGACACTGGCGTAAGAACCGACTAACGTCCCCATATTGATGTTTACCGCCGAAGTGGGATCGGCCATCTGACGAACGTTGGTGTCAGTACCGGAGCGGGTATCTTTCTGACTTTGTTCGG  >  NC_000913/1072845‑1073140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑