BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,109,596	T→C	Y88H (TAC→CAC)	opgG →	OPG biosynthetic periplasmic beta‑1,6 branching glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,109,596	0	T	C	100.0%	606.2 / NA	152	Y88H (TAC→CAC)	opgG	OPG biosynthetic periplasmic beta‑1,6 branching glycosyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (79/73); total (79/73)

Only 100 of 152 total aligned reads displayed.

CTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTT  >  NC_000913/1109451‑1109743

cTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTc                                                                                                                                                >  4:339600/1‑151 (MQ=255)

 tACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCg                                                                                                                                               >  1:86812/1‑151 (MQ=255)

  aCGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTc                                                                                                                                                <  2:993765/149‑1 (MQ=255)

   cGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGAt                                                                                                                                             <  4:448994/151‑1 (MQ=255)

      gaCGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATAcc                                                                                                                                          >  4:826943/1‑151 (MQ=255)

      gaCGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATAcc                                                                                                                                          <  3:471628/151‑1 (MQ=255)

         gCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCg                                                                                                                                       <  2:1117571/151‑1 (MQ=255)

           ccAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGt                                                                                                                                     <  4:924009/151‑1 (MQ=255)

           ccAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGt                                                                                                                                     <  2:795391/151‑1 (MQ=255)

               aaGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCaaa                                                                                                                                 <  2:1676433/151‑1 (MQ=255)

               aaGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCaaa                                                                                                                                 <  1:238394/151‑1 (MQ=255)

               aaGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCaa                                                                                                                                  >  4:256789/1‑150 (MQ=255)

                 gCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAaaat                                                                                                                               <  1:159914/151‑1 (MQ=255)

                  cAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAaaat                                                                                                                               >  4:1611225/1‑150 (MQ=255)

                   aaCTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAaaata                                                                                                                              >  2:69789/1‑150 (MQ=255)

                      ttGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATg                                                                                                                          >  2:1478731/1‑151 (MQ=255)

                       tGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATg                                                                                                                          >  2:814523/1‑150 (MQ=255)

                       tGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATg                                                                                                                          <  2:1359589/150‑1 (MQ=255)

                         cccTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGa                                                                                                                         >  2:530277/1‑149 (MQ=255)

                          ccTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGt                                                                                                                      <  4:152541/151‑1 (MQ=255)

                             ccGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTg                                                                                                                     <  4:1532488/149‑1 (MQ=255)

                             ccGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTg                                                                                                                     <  3:475056/149‑1 (MQ=255)

                             ccGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTg                                                                                                                     <  2:570065/149‑1 (MQ=255)

                              cGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACt                                                                                                                  <  4:236295/151‑1 (MQ=255)

                              cGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACt                                                                                                                  <  3:345425/151‑1 (MQ=255)

                              cGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACt                                                                                                                  <  4:839039/151‑1 (MQ=255)

                               gTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTg                                                                                                                 >  2:80129/1‑151 (MQ=255)

                                 tttCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGcc                                                                                                               >  3:328523/1‑151 (MQ=255)

                                      gcgATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAGGTGACTGCCACCGc                                                                                                          >  4:609445/1‑151 (MQ=255)

                                       cgATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCa                                                                                                         <  1:228384/151‑1 (MQ=255)

                                        gATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGc                                                                                                          >  3:306596/1‑149 (MQ=255)

                                         atatGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGt                                                                                                       >  2:863452/1‑151 (MQ=255)

                                          tatGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTc                                                                                                      >  1:352036/1‑151 (MQ=255)

                                           atGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTc                                                                                                      <  1:433245/150‑1 (MQ=255)

                                           atGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCa                                                                                                     <  1:112518/151‑1 (MQ=255)

                                             gAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCaa                                                                                                    >  1:448425/1‑150 (MQ=255)

                                              aaaTACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTAGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAAc                                                                                                  <  4:1147417/151‑1 (MQ=255)

                                               taTACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACg                                                                                                 >  2:740357/2‑151 (MQ=255)

                                               aaTACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACg                                                                                                 <  4:1399975/151‑1 (MQ=255)

                                                  aCGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGaa                                                                                               <  1:31090/150‑1 (MQ=255)

                                                  aCGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAAt                                                                                              <  4:1608532/151‑1 (MQ=255)

                                                   cgcgGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCTAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATc                                                                                             <  1:253974/151‑1 (MQ=255)

                                                    gcgGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATc                                                                                             >  2:1302708/1‑150 (MQ=255)

                                                     cgGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACGATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCa                                                                                            >  4:248525/1‑150 (MQ=255)

                                                       gACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAAt                                                                                         <  1:264661/151‑1 (MQ=255)

                                                       gACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGAATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAAt                                                                                         >  4:1380489/1‑151 (MQ=255)

                                                           aTCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATAc                                                                                       <  4:1253383/149‑1 (MQ=255)

                                                           aTCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATAc                                                                                       <  4:1660008/149‑1 (MQ=255)

                                                           aTCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATAc                                                                                       >  4:1162981/1‑149 (MQ=255)

                                                           aTCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATAc                                                                                       >  2:335766/1‑149 (MQ=255)

                                                             cagcagATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGcc                                                                                   <  2:476638/151‑1 (MQ=255)

                                                              agcagATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGccc                                                                                  <  4:368827/151‑1 (MQ=255)

                                                                cagATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCgg                                                                                >  1:170246/1‑151 (MQ=255)

                                                                 agATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGa                                                                               <  2:1451791/151‑1 (MQ=255)

                                                                  gATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGat                                                                              <  4:633037/151‑1 (MQ=255)

                                                                     ccAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGattat                                                                           >  2:688078/1‑151 (MQ=255)

                                                                     ccAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGattat                                                                           >  2:1204998/1‑151 (MQ=255)

                                                                     ccAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGattat                                                                           >  2:1414836/1‑151 (MQ=255)

                                                                        gTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTc                                                                        >  3:166740/1‑151 (MQ=255)

                                                                         tttAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTc                                                                        >  1:276131/1‑150 (MQ=255)

                                                                           tAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACt                                                                     >  4:1104638/1‑151 (MQ=255)

                                                                             aTCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACttt                                                                   >  4:467019/1‑151 (MQ=255)

                                                                              tCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTc                                                                  >  4:753670/1‑151 (MQ=255)

                                                                               cATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTc                                                                  <  2:1271131/150‑1 (MQ=255)

                                                                               cATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCg                                                                 >  4:25955/1‑151 (MQ=255)

                                                                               cATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCg                                                                 <  1:311678/151‑1 (MQ=255)

                                                                                  gACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGc                                                               <  2:1458469/150‑1 (MQ=255)

                                                                                   aCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCg                                                              <  4:1243188/150‑1 (MQ=255)

                                                                                     aaaGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATg                                                           >  3:448688/1‑151 (MQ=255)

                                                                                      aaGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGt                                                          >  4:1553546/1‑151 (MQ=255)

                                                                                       aGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGtt                                                         >  2:1482210/1‑151 (MQ=255)

                                                                                        gCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTc                                                        >  2:1355720/1‑151 (MQ=255)

                                                                                         cGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTc                                                        >  4:274842/1‑150 (MQ=255)

                                                                                         cGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCa                                                       <  2:256377/151‑1 (MQ=255)

                                                                                               ggAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATg                                                  <  4:312944/150‑1 (MQ=255)

                                                                                               ggAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGa                                                 <  4:1349293/151‑1 (MQ=255)

                                                                                                   cAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACaaa                                             <  4:480161/151‑1 (MQ=255)

                                                                                                    aaTCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAg                                            <  2:1609262/151‑1 (MQ=255)

                                                                                                     aTCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACaa                                              >  4:1251927/1‑148 (MQ=255)

                                                                                                     aTCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACaa                                              >  2:1132285/1‑148 (MQ=255)

                                                                                                        tGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGGCAAAGATAc                                        >  2:1205736/1‑151 (MQ=255)

                                                                                                         gAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGAt                                          <  4:621316/148‑1 (MQ=255)

                                                                                                           aGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGt                                     >  3:203445/1‑151 (MQ=255)

                                                                                                            gACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTa                                    <  1:40860/151‑1 (MQ=255)

                                                                                                             aCCCCATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTaa                                   <  2:1650434/151‑1 (MQ=255)

                                                                                                                ccATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTaaa                                  >  3:230825/1‑149 (MQ=255)

                                                                                                                ccATTCAAACTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAg                                <  2:1006087/151‑1 (MQ=255)

                                                                                                                      aaaCTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTg                          >  4:1549950/1‑151 (MQ=255)

                                                                                                                       aaCTCGAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTgg                         >  3:123971/1‑151 (MQ=255)

                                                                                                                            gAGTTCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTg                    <  2:533507/151‑1 (MQ=255)

                                                                                                                               ttCTACCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCg                 >  3:359228/1‑151 (MQ=255)

                                                                                                                                   aCCATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGttt             >  4:526280/1‑151 (MQ=255)

                                                                                                                                    ccATCAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGtttt            >  2:1643027/1‑151 (MQ=255)

                                                                                                                                        cAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTaaa         <  3:170260/150‑1 (MQ=255)

                                                                                                                                        cAGGGTATGCACTTCGATACCCCGGTCAAAATAAATGAAATGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTaaa         <  2:254747/150‑1 (MQ=255)

                                                                                                                                          gggTATGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTg      <  2:1551630/151‑1 (MQ=255)

                                                                                                                                              aTGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCttt  >  2:854965/1‑151 (MQ=255)

                                                                                                                                              aTGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCttt  >  2:1683519/1‑151 (MQ=255)

                                                                                                                                              aTGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCtt   <  4:1645031/150‑1 (MQ=255)

                                                                                                                                              aTGCACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCtt   <  3:411823/150‑1 (MQ=255)

CTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGATTATTTCACTTTCGGCGATGTTCAGCATGACAAAGATACGGTAAAAGACCTTGGCTTTGCCGGTTTTAAAGTGCTTT  >  NC_000913/1109451‑1109743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`