Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,111,899 A→G D346G (GAT→GGT)  opgH → OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,111,8990AG100.0% 427.2 / NA 113D346G (GAT→GGT) opgHOPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (56/57);  total (56/57)

Only 100 of 114 total aligned reads displayed.

AAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGC  >  NC_000913/1111752‑1112044
                                                                                                                                                   |                                                                                                                                                 
aaGCCTGGATGGCGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGCAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTg                                                                                                                                                 >  2:426249/1‑150 (MQ=255)
 aGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCt                                                                                                                                               >  4:240550/1‑151 (MQ=255)
 aGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCt                                                                                                                                               <  2:715144/151‑1 (MQ=255)
  gCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCta                                                                                                                                              <  2:442826/151‑2 (MQ=255)
  gCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGCCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGTTGGGTGCTg                                                                                                                                              >  2:713563/1‑151 (MQ=255)
   ccTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTggtgctgggtgctg                                                                                                                                              >  2:1267130/1‑150 (MQ=255)
      ggatggaGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGAc                                                                                                                                            <  4:819828/149‑1 (MQ=255)
      ggatggaGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTc                                                                                                                                          <  2:618881/151‑1 (MQ=255)
      ggatggaGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTc                                                                                                                                          <  2:1303083/151‑1 (MQ=255)
       gatggaGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCg                                                                                                                                         >  4:491404/1‑151 (MQ=255)
          ggaGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGt                                                                                                                                       <  2:43774/150‑1 (MQ=255)
            aGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAAt                                                                                                                                    <  2:1513922/151‑1 (MQ=255)
            aGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAAt                                                                                                                                    <  3:212460/151‑1 (MQ=255)
              cTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATg                                                                                                                                   >  2:379101/1‑150 (MQ=255)
                      tGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGAt                                                                                                                          >  1:81006/1‑151 (MQ=255)
                        aaGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGAttg                                                                                                                        >  4:739278/1‑151 (MQ=255)
                             ggTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGAttgtttgt                                                                                                                   <  4:1371475/151‑1 (MQ=255)
                                  cGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCggg                                                                                                              <  4:282050/151‑1 (MQ=255)
                                    aaGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCt                                                                                                            <  4:1633191/151‑1 (MQ=255)
                                     aGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTg                                                                                                           >  3:443420/1‑151 (MQ=255)
                                     aGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTg                                                                                                           >  1:290962/1‑151 (MQ=255)
                                      ggTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTgg                                                                                                          >  4:1466345/1‑151 (MQ=255)
                                       gTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGt                                                                                                         <  2:354754/151‑1 (MQ=255)
                                        tCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTg                                                                                                        >  2:764984/1‑151 (MQ=255)
                                        tCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTg                                                                                                        >  4:345299/1‑151 (MQ=255)
                                          aGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTgcg                                                                                                      <  1:406473/151‑1 (MQ=255)
                                           gATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTgcgc                                                                                                     >  3:190711/1‑151 (MQ=255)
                                              tttCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTTGGTGCTGACTCGGTAATGTCCGGTGATTGTTTGTGCGGGCTGGTGCGCCt                                                                                                   <  4:66213/150‑1 (MQ=255)
                                              tttCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCt                                                                                                   <  4:905746/150‑1 (MQ=255)
                                               ttCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTg                                                                                                  >  4:291818/1‑150 (MQ=255)
                                                tCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGa                                                                                                 <  2:306314/150‑1 (MQ=255)
                                                  tATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCGGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATgg                                                                                              >  2:1453161/1‑151 (MQ=255)
                                                  tATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATgg                                                                                              >  2:1278426/1‑151 (MQ=255)
                                                   aTCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATgg                                                                                              >  4:1207448/1‑150 (MQ=255)
                                                   aTCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATgg                                                                                              <  4:344514/150‑1 (MQ=255)
                                                   aTCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATg                                                                                               <  4:941891/149‑1 (MQ=255)
                                                   aTCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATg                                                                                               <  2:239079/149‑1 (MQ=255)
                                                    tCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGa                                                                                             >  4:1490645/1‑150 (MQ=255)
                                                      gccgccgccGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGc                                                                                          >  1:115497/1‑151 (MQ=255)
                                                       ccgccgccgTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGcc                                                                                         <  2:1176649/151‑1 (MQ=255)
                                                         gccgccgTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCa                                                                                        >  2:14635/1‑150 (MQ=255)
                                                          ccgccgTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAAc                                                                                      >  4:1206362/1‑151 (MQ=255)
                                                          ccgccgTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAAc                                                                                      <  4:1065627/151‑1 (MQ=255)
                                                                 cgccgcGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAAc                                                                                <  2:994569/150‑1 (MQ=255)
                                                                 cgccgcGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAAc                                                                                <  2:1673603/150‑1 (MQ=255)
                                                                  gccgcGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACg                                                                               <  2:240167/150‑1 (MQ=255)
                                                                   ccgcGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGcc                                                                             >  4:1031567/1‑151 (MQ=255)
                                                                    cgcgTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCg                                                                            <  3:374129/151‑1 (MQ=255)
                                                                      cgTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCggg                                                                          <  2:1562918/151‑1 (MQ=255)
                                                                        tGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGAt                                                                        >  2:583539/1‑151 (MQ=255)
                                                                        tGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGAt                                                                        >  4:1666429/1‑151 (MQ=255)
                                                                         gAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGAt                                                                        <  3:476078/150‑1 (MQ=255)
                                                                          aaGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCa                                                                      <  4:593924/151‑1 (MQ=255)
                                                                             cGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTc                                                                   >  4:1086540/1‑151 (MQ=255)
                                                                             cGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTc                                                                   >  2:27607/1‑151 (MQ=255)
                                                                              gTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGAttgtt                                                                                                                      >  3:218609/1‑99 (MQ=255)
                                                                              gTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGAttgtt                                                                                                                      <  1:218594/99‑1 (MQ=255)
                                                                                aaaaGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAgt                                                                >  4:940149/1‑151 (MQ=255)
                                                                                  aaGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAgtcg                                                              >  2:36335/1‑151 (MQ=255)
                                                                                    gCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAgtcgtc                                                            <  4:1078378/151‑1 (MQ=255)
                                                                                     cGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGtcgtcg                                                           <  2:105974/151‑1 (MQ=255)
                                                                                          atatCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGaa                                                      <  4:584348/151‑1 (MQ=255)
                                                                                           tatCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGATCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGaa                                                      >  4:1448075/1‑150 (MQ=255)
                                                                                           tatCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGaa                                                      >  1:106915/1‑150 (MQ=255)
                                                                                           tatCGATGACTTCTGCCGTCGCGGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGaa                                                      >  2:1296573/1‑150 (MQ=255)
                                                                                            atCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAg                                                    <  2:662671/151‑1 (MQ=255)
                                                                                            atCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAg                                                    <  4:541498/151‑1 (MQ=255)
                                                                                              cGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGc                                                   <  4:1656831/150‑1 (MQ=255)
                                                                                              cGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCg                                                  <  1:462979/151‑1 (MQ=255)
                                                                                              cGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCg                                                  >  4:574766/1‑151 (MQ=255)
                                                                                                 tGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTcc                                               >  4:1316428/1‑151 (MQ=255)
                                                                                                  gACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCg                                              <  4:6208/151‑1 (MQ=255)
                                                                                                    cTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGt                                            >  3:165858/1‑151 (MQ=255)
                                                                                                     ttCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGt                                            >  4:1554884/1‑150 (MQ=255)
                                                                                                       cTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATg                                         >  4:1559138/1‑151 (MQ=255)
                                                                                                         gCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATgg                                        >  4:341035/1‑150 (MQ=255)
                                                                                                         gCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATg                                         <  4:844348/149‑1 (MQ=255)
                                                                                                            gTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATAc                                    >  4:108144/1‑151 (MQ=255)
                                                                                                             tCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACg                                   >  3:209033/1‑151 (MQ=255)
                                                                                                              cGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACg                                   <  3:427611/150‑1 (MQ=255)
                                                                                                                 tGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGt                               >  4:103830/1‑151 (MQ=255)
                                                                                                                 tGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGt                               >  4:1120396/1‑151 (MQ=255)
                                                                                                                 tGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGt                               >  2:1652157/1‑151 (MQ=255)
                                                                                                                  ggggCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGt                               <  4:165838/150‑1 (MQ=255)
                                                                                                                  ggggCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTa                              <  1:390666/151‑1 (MQ=255)
                                                                                                                  ggggCAGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTa                              <  2:564791/151‑1 (MQ=255)
                                                                                                                       aGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATgcgc                         >  4:858223/1‑151 (MQ=255)
                                                                                                                       aGCCAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATgcgc                         >  3:116464/1‑151 (MQ=255)
                                                                                                                          cAGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCt                      <  2:738289/151‑1 (MQ=255)
                                                                                                                           aGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTg                     >  3:441416/1‑151 (MQ=255)
                                                                                                                           aGTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTg                     <  2:127007/151‑1 (MQ=255)
                                                                                                                            gTACAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGt                    >  2:893683/1‑151 (MQ=255)
                                                                                                                               cAGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTcag                 <  3:149592/151‑1 (MQ=255)
                                                                                                                                aGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTcagc                <  3:88971/151‑1 (MQ=255)
                                                                                                                                aGCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGCATGGATACGCTGTATGCGCGCTGTcagc                >  2:1529424/1‑151 (MQ=255)
                                                                                                                                 gCTACATGGTGGTGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTcagca               <  3:81010/151‑1 (MQ=255)
                                                                                                                                        ggtggtGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTcgcg        <  2:616072/151‑1 (MQ=255)
                                                                                                                                        ggtggtGCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTcgc         <  3:409751/150‑1 (MQ=255)
                                                                                                                                            gtgCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGAccc    >  4:1636175/1‑151 (MQ=255)
                                                                                                                                              gCTGGGTGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCcgc  <  4:205197/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGGAAGCCAACCCGAACGCCGGGATCATTCAGTCGTCGCCGAAAGCGTCCGGTATGGATACGCTGTATGCGCGCTGTCAGCAGTTCGCGACCCGC  >  NC_000913/1111752‑1112044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: