Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,228,980 C→T R56C (CGC→TGC)  ycgN → UPF0153 family cysteine cluster protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,228,9800CT99.1% 433.9 / NA 112R56C (CGC→TGC) ycgNUPF0153 family cysteine cluster protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (58/53);  total (59/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

GAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTCGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCTTATCGTTTGCTGGCGGAAGG  >  NC_000913/1228832‑1229125
                                                                                                                                                    |                                                                                                                                                 
gaGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGAGGCGGAATGGGAGTCGTTGTGAGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTCGc                                                                                                                                                 >  4:532810/1‑151 (MQ=255)
 aGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGcc                                                                                                                                                >  4:1347407/1‑151 (MQ=255)
     aTGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCt                                                                                                                                            <  4:1319971/151‑1 (MQ=255)
      tGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTc                                                                                                                                           >  2:520274/1‑151 (MQ=255)
           cTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAatat                                                                                                                                      >  2:274235/1‑151 (MQ=255)
            tttCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATAtt                                                                                                                                     >  2:775542/1‑151 (MQ=255)
            tttCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATAtt                                                                                                                                     >  4:1561116/1‑151 (MQ=255)
             ttCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATAtt                                                                                                                                     <  4:1563434/150‑1 (MQ=255)
                 ggCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAAc                                                                                                                                >  4:1202803/1‑151 (MQ=255)
                   cAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCt                                                                                                                              <  4:104767/151‑1 (MQ=255)
                     aaGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTTAATATTAAAACCTGt                                                                                                                            <  2:1482988/151‑1 (MQ=255)
                       gTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCa                                                                                                                          <  4:1686395/151‑1 (MQ=255)
                        tAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCa                                                                                                                          >  4:610400/1‑150 (MQ=255)
                         aaaaCCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCaa                                                                                                                         <  4:816783/150‑1 (MQ=255)
                         aaaaCCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCaa                                                                                                                         <  2:902151/150‑1 (MQ=255)
                         aaaaCCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAAt                                                                                                                        >  2:147737/1‑151 (MQ=255)
                         aaaaCCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAAt                                                                                                                        <  2:1665435/151‑1 (MQ=255)
                          aaaCCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATg                                                                                                                       >  4:437251/1‑151 (MQ=255)
                            aCCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTc                                                                                                                     <  4:222176/151‑1 (MQ=255)
                             cccTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGAGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCg                                                                                                                    >  2:565492/1‑151 (MQ=255)
                              ccTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCgg                                                                                                                   <  2:775402/151‑1 (MQ=255)
                               cTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACCCCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTc                                                                                                                     <  3:29458/148‑1 (MQ=255)
                                tGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGa                                                                                                                  >  2:199972/1‑150 (MQ=255)
                                tGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGa                                                                                                                  >  1:424498/1‑150 (MQ=255)
                                 ggACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAAc                                                                                                                <  4:789183/151‑1 (MQ=255)
                                    cGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTAc                                                                                                             >  4:251633/1‑151 (MQ=255)
                                     gAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACg                                                                                                            >  2:828282/1‑151 (MQ=255)
                                     gAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACg                                                                                                            >  4:452800/1‑151 (MQ=255)
                                        aTGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAAc                                                                                                         <  4:1596094/151‑1 (MQ=255)
                                        aTGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAAc                                                                                                         <  4:1242978/151‑1 (MQ=255)
                                          gAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAAcg                                                                                                        <  4:772272/150‑1 (MQ=255)
                                           aGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAAcgtc                                                                                                      <  1:233670/151‑1 (MQ=255)
                                           aGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAAcgtc                                                                                                      >  4:276537/1‑151 (MQ=255)
                                            gCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAAcgtcg                                                                                                     <  4:256767/151‑1 (MQ=255)
                                            gCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAAcgtcg                                                                                                     >  1:415549/1‑151 (MQ=255)
                                               aTGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGttt                                                                                                  <  4:200302/151‑1 (MQ=255)
                                               aTGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGttt                                                                                                  >  4:793175/1‑151 (MQ=255)
                                                tGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTc                                                                                                 >  4:10/1‑151 (MQ=255)
                                                 gCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTc                                                                                                 <  4:1028359/150‑1 (MQ=255)
                                                 gCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCg                                                                                                <  2:625505/151‑1 (MQ=255)
                                                 gCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCg                                                                                                <  3:459373/151‑1 (MQ=255)
                                                   ggAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAg                                                                                              <  1:309036/151‑1 (MQ=255)
                                                    gAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGt                                                                                             >  2:13296/1‑151 (MQ=255)
                                                          gAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAAc                                                                                       >  2:40118/1‑151 (MQ=255)
                                                               gTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGAc                                                                                  >  4:1686937/1‑151 (MQ=255)
                                                                ttGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACt                                                                                 >  4:136761/1‑151 (MQ=255)
                                                                 tgtgtgATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTg                                                                                >  4:518756/1‑151 (MQ=255)
                                                                  gtgtgATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTg                                                                                <  2:1587406/150‑1 (MQ=255)
                                                                  gtgtgATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTg                                                                                <  4:1629918/150‑1 (MQ=255)
                                                                   tgtgATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGc                                                                               >  2:757162/1‑150 (MQ=255)
                                                                    gtgATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCat                                                                             <  1:409472/151‑1 (MQ=255)
                                                                       aTGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAGCCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCattaa                                                                          <  1:353204/151‑1 (MQ=255)
                                                                         ggTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCattaaat                                                                        <  4:881017/151‑1 (MQ=255)
                                                                          gTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCattaaatt                                                                       <  2:819474/151‑1 (MQ=255)
                                                                          gTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCattaaatt                                                                       >  2:125867/1‑151 (MQ=255)
                                                                           ttGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCattaaatt                                                                       >  2:446853/1‑150 (MQ=255)
                                                                           ttGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCattaaatt                                                                       <  2:387846/150‑1 (MQ=255)
                                                                           ttGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCattaaatt                                                                       >  2:1010591/1‑150 (MQ=255)
                                                                               ggTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAAccc                                                                  >  3:399121/1‑151 (MQ=255)
                                                                                 tCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCg                                                                 >  2:590079/1‑150 (MQ=255)
                                                                                    gtgtTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGa                                                              >  1:332933/1‑150 (MQ=255)
                                                                                    gtgtTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGa                                                              >  4:1417355/1‑150 (MQ=255)
                                                                                     tgtTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGaa                                                             <  3:129715/150‑1 (MQ=255)
                                                                                         gCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATc                                                         <  2:1375089/150‑1 (MQ=255)
                                                                                          ccTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCt                                                        >  4:1420085/1‑150 (MQ=255)
                                                                                             gCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCa                                                    <  2:827664/151‑1 (MQ=255)
                                                                                             gCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCa                                                    <  4:1232077/151‑1 (MQ=255)
                                                                                                  aaCTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACAtt                                               >  2:141146/1‑151 (MQ=255)
                                                                                                       atggatgAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGaa                                           <  4:1430210/150‑1 (MQ=255)
                                                                                                       atggatgAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAAt                                          <  3:424516/151‑1 (MQ=255)
                                                                                                       atggatgAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAAt                                          <  4:324322/151‑1 (MQ=255)
                                                                                                       atggatgAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAAt                                          >  2:60938/1‑151 (MQ=255)
                                                                                                          gatgAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGc                                       >  2:941401/1‑151 (MQ=255)
                                                                                                              aaGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGcc                                   <  4:66050/151‑1 (MQ=255)
                                                                                                               aGACACCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGcc                                   >  3:46555/1‑150 (MQ=255)
                                                                                                                  cacCGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATg                               >  4:153924/1‑151 (MQ=255)
                                                                                                                    ccGACGAAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGAc                             >  2:640137/1‑151 (MQ=255)
                                                                                                                     cgacgaAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGAcc                            >  3:85227/1‑151 (MQ=255)
                                                                                                                      gacgaAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCt                           <  2:792134/151‑1 (MQ=255)
                                                                                                                         gaAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCTATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTgcg                        <  2:516118/151‑1 (MQ=255)
                                                                                                                         gaAATCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTgcg                        >  4:797139/1‑151 (MQ=255)
                                                                                                                           aaTCTACTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCt                      <  4:1013215/151‑1 (MQ=255)
                                                                                                                                aCTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAATATTCGAATGGCTGCCAATGACCTGCGCTTATCg                 <  2:1216815/151‑1 (MQ=255)
                                                                                                                                aCTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCtt                     <  4:829377/147‑1 (MQ=255)
                                                                                                                                aCTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCTTa                    <  3:397846/148‑1 (MQ=255)
                                                                                                                                aCTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCTTAt                   <  4:1235889/149‑1 (MQ=255)
                                                                                                                                aCTTCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCTTATCg                 <  4:417732/151‑1 (MQ=255)
                                                                                                                                   tCACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCTTATCg                 <  2:1201295/148‑1 (MQ=255)
                                                                                                                                    cACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCTTATCGttt              <  4:1603170/150‑1 (MQ=255)
                                                                                                                                    cACTAACGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATAACCTGCGCTTATCGTTTg             <  1:182337/151‑1 (MQ=255)
                                                                                                                                      cTAACGTCGACTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCTTATCGTTTGCt           >  2:17692/1‑151 (MQ=255)
                                                                                                                                        aaCGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCTTATCGTTTGCt           <  4:447487/149‑1 (MQ=255)
                                                                                                                                        aaCGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCTTATCGTTTGCTgg         <  4:1575573/151‑1 (MQ=255)
                                                                                                                                        aaCGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCTTATCGTTTGCTgg         >  4:753311/1‑151 (MQ=255)
                                                                                                                                        aaCGTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCTTATCGTTTGCTgg         >  2:63668/1‑151 (MQ=255)
                                                                                                                                         aCGCCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCTTATCGTTTGCTGGc        >  2:263989/1‑151 (MQ=255)
                                                                                                                                           gTCGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCTTATCGTTTGCTGGCgg      >  4:44857/1‑151 (MQ=255)
                                                                                                                                             cGCCTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCTTATCGTTTGCTGGCGGa     >  4:484878/1‑150 (MQ=255)
                                                                                                                                                cTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCTTATCGTTTGCTGGCGGAAgg  >  4:1290892/1‑150 (MQ=255)
                                                                                                                                                cTGTTGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCTTATCGTTTGCTGGCGGAAgg  >  4:225001/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTCGCCAGCTCAATATTAAAACCTGTCAATGTCGGAACTACGAACGTCGTTTCGAGTTTGAACCCGACTGCATTAAATTAACCCGTGAAAATCTGCCAACATTCGAATGGCTGCCAATGACCTGCGCTTATCGTTTGCTGGCGGAAGG  >  NC_000913/1228832‑1229125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑