BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,307,362	C→T	W428* (TGG→TGA)	clsA ←	cardiolipin synthase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,307,362	0	C	T	99.1%	427.5 / ‑4.6	113	W428* (TGG→TGA)	clsA	cardiolipin synthase 1
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (55/57); total (55/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

Only 100 of 113 total aligned reads displayed.

TCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGC  >  NC_000913/1307215‑1307508

tCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTcac                                                                                                                                                 >  4:20416/1‑151 (MQ=255)

tCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTcac                                                                                                                                                 >  2:760521/1‑151 (MQ=255)

 cGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTcac                                                                                                                                                 >  2:85907/1‑150 (MQ=255)

  gCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTcaca                                                                                                                                                <  2:350725/150‑1 (MQ=255)

  gCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAg                                                                                                                                               <  4:3215/151‑1 (MQ=255)

     cGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGa                                                                                                                                              <  3:132721/149‑1 (MQ=255)

     cGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGAc                                                                                                                                             <  4:1257970/150‑1 (MQ=255)

     cGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGAc                                                                                                                                             <  4:103872/150‑1 (MQ=255)

      ggCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACt                                                                                                                                            >  4:1557388/1‑150 (MQ=255)

        cGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACg                                                                                                                                         >  2:1072042/1‑151 (MQ=255)

          acacGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCa                                                                                                                                       <  4:161735/151‑1 (MQ=255)

            acGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCat                                                                                                                                      >  3:144859/1‑150 (MQ=255)

                tGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCa                                                                                                                                  >  2:427474/1‑150 (MQ=255)

                tGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCa                                                                                                                                  <  3:429666/150‑1 (MQ=255)

                 gCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCaa                                                                                                                                 <  1:299494/150‑1 (MQ=255)

                  ccATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAgg                                                                                                                               >  3:207114/1‑151 (MQ=255)

                  ccATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAgg                                                                                                                               <  4:781965/151‑1 (MQ=255)

                    aTAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGtt                                                                                                                             <  2:823307/151‑1 (MQ=255)

                    aTAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGtt                                                                                                                             <  4:1656410/151‑1 (MQ=255)

                         ggACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACtg                                                                                                                        <  4:1165899/151‑1 (MQ=255)

                         ggACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACt                                                                                                                         <  3:313475/150‑1 (MQ=255)

                          gACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACtgt                                                                                                                       >  1:66027/1‑151 (MQ=255)

                           aCGTTTTATCCAGAAACGGGCATCGAGCAGACGTGAACGCGAAATATATTCGCCCTGAAAGGCGGCGAGCTCAGCTCCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAACTTTAGTCACAGACTACGCATATCATTGTTAACtg                                                                                                                        <  2:1017051/149‑1 (MQ=255)

                            cGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGc                                                                                                                     >  2:441579/1‑151 (MQ=255)

                             gTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGcc                                                                                                                    >  4:547768/1‑151 (MQ=255)

                                    ccATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGa                                                                                                             <  1:440403/151‑1 (MQ=255)

                                     cATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGAc                                                                                                            <  4:1639880/151‑1 (MQ=255)

                                        aaaCGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACtt                                                                                                          >  3:283089/1‑150 (MQ=255)

                                           tgggCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGtt                                                                                                      >  1:658/2‑151 (MQ=255)

                                           tgggCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGtt                                                                                                      >  1:345608/2‑151 (MQ=255)

                                           cGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGtt                                                                                                      >  4:568429/1‑151 (MQ=255)

                                            gggCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTc                                                                                                     >  2:978609/1‑151 (MQ=255)

                                                aTCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCGGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCa                                                                                                 <  1:178678/151‑1 (MQ=255)

                                                  cGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATc                                                                                               <  2:993813/151‑1 (MQ=255)

                                                   gAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCg                                                                                              >  4:296898/1‑151 (MQ=255)

                                                    aGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGa                                                                                             <  1:355883/151‑1 (MQ=255)

                                                       aGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGAcc                                                                                           >  4:1125037/1‑150 (MQ=255)

                                                       aGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGAcc                                                                                           >  4:1520763/1‑150 (MQ=255)

                                                        gACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAg                                                                                         <  4:465258/151‑1 (MQ=255)

                                                            tGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACg                                                                                     >  4:418401/1‑151 (MQ=255)

                                                             gAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCGGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGc                                                                                    >  4:382300/1‑151 (MQ=255)

                                                                 gcgAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCtt                                                                                <  3:448371/151‑1 (MQ=255)

                                                                   gAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTgg                                                                              >  4:338608/1‑151 (MQ=255)

                                                                     aaTATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGt                                                                             >  2:152860/1‑150 (MQ=255)

                                                                       tataATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATg                                                                          >  2:430327/1‑151 (MQ=255)

                                                                           aTCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGt                                                                      >  3:254321/1‑151 (MQ=255)

                                                                           aTCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGt                                                                      >  4:873824/1‑151 (MQ=255)

                                                                              gTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAAc                                                                   >  4:251414/1‑151 (MQ=255)

                                                                               tCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAAcc                                                                  >  3:215431/1‑151 (MQ=255)

                                                                                ccTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAAccc                                                                 >  3:371138/1‑151 (MQ=255)

                                                                                   gAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGc                                                               <  1:110036/150‑1 (MQ=255)

                                                                                     aCGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCtt                                                            <  2:1102235/151‑1 (MQ=255)

                                                                                      cggcggCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTc                                                           <  4:659748/151‑1 (MQ=255)

                                                                                      cggcggCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTc                                                           >  1:38064/1‑151 (MQ=255)

                                                                                      cggcggCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTc                                                           <  1:76337/151‑1 (MQ=255)

                                                                                      cggcggCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTc                                                           <  2:1397437/151‑1 (MQ=255)

                                                                                      cggcggCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTc                                                           >  4:736173/1‑151 (MQ=255)

                                                                                       ggcggcGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTc                                                           >  4:1670985/1‑150 (MQ=255)

                                                                                       ggcggcGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCa                                                          <  2:324470/151‑1 (MQ=255)

                                                                                        gcggcgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCaa                                                         <  3:65032/151‑1 (MQ=255)

                                                                                         cggcgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCaaa                                                        <  4:1440205/151‑1 (MQ=255)

                                                                                         cggcgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCaaa                                                        <  4:1342760/151‑1 (MQ=255)

                                                                                          ggcgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAAc                                                       >  2:1480872/1‑151 (MQ=255)

                                                                                            cgAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTg                                                     >  4:199567/1‑151 (MQ=255)

                                                                                                gTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATaa                                                 <  4:1313211/151‑1 (MQ=255)

                                                                                                 tCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATaa                                                 >  3:473462/1‑150 (MQ=255)

                                                                                                 tCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATaa                                                 >  4:940683/1‑150 (MQ=255)

                                                                                                  cAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATaaa                                                <  3:249102/150‑1 (MQ=255)

                                                                                                  cAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATaaa                                                <  1:376188/150‑1 (MQ=255)

                                                                                                   aGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAAtt                                              <  3:441715/151‑1 (MQ=255)

                                                                                                   aGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAAtt                                              <  4:1147539/151‑1 (MQ=255)

                                                                                                     cACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAAtttt                                            <  4:983795/151‑1 (MQ=255)

                                                                                                        cAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAAc                                         <  4:388176/151‑1 (MQ=255)

                                                                                                          aaaCCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAAccc                                       >  3:71676/1‑151 (MQ=255)

                                                                                                            aCCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCcca                                     <  3:29225/151‑1 (MQ=255)

                                                                                                                 tATCGTCGATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcag                                 <  2:338099/150‑1 (MQ=255)

                                                                                                                    cgtcgATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagccag                             <  2:260683/151‑1 (MQ=255)

                                                                                                                      tcgATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagccagc                            >  4:117508/1‑150 (MQ=255)

                                                                                                                      tcgATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCccagcagccagc                            >  3:71214/1‑150 (MQ=255)

                                                                                                                        gATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGt                         >  4:724375/1‑151 (MQ=255)

                                                                                                                        gATTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGt                         >  2:185178/1‑151 (MQ=255)

                                                                                                                         aTTGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGtt                        <  4:1073407/151‑1 (MQ=255)

                                                                                                                          ttGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTc                       >  4:1051773/1‑151 (MQ=255)

                                                                                                                           tGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTcc                      >  2:854093/1‑151 (MQ=255)

                                                                                                                           tGCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTc                       <  2:1543562/150‑1 (MQ=255)

                                                                                                                            gCCAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTcc                      <  2:749531/150‑1 (MQ=255)

                                                                                                                             ccAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGt                    <  3:284718/151‑1 (MQ=255)

                                                                                                                              cAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGt                    >  3:13959/1‑150 (MQ=255)

                                                                                                                              cAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTa                   <  2:1524571/151‑1 (MQ=255)

                                                                                                                              cAGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTa                   <  1:469580/151‑1 (MQ=255)

                                                                                                                               aGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTa                   >  4:472745/1‑150 (MQ=255)

                                                                                                                               aGGGTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTa                   >  1:178174/1‑150 (MQ=255)

                                                                                                                                gggTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTaa                  >  2:763302/1‑150 (MQ=255)

                                                                                                                                  gTAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTaa                  >  4:973163/1‑148 (MQ=255)

                                                                                                                                   tAATCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGa               >  3:54576/1‑150 (MQ=255)

                                                                                                                                    aaTCTCGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAAt             <  2:828886/151‑1 (MQ=255)

                                                                                                                                         cGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGcgcg        <  3:115750/151‑1 (MQ=255)

                                                                                                                                         cGAAATTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGcgcg        >  2:401826/1‑151 (MQ=255)

                                                                                                                                             aTTTAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTg    <  2:20380/151‑1 (MQ=255)

                                                                                                                                               ttAGTCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGc  >  2:577766/1‑151 (MQ=255)

TCGCTCGGCGACACGTTGCCATAATGGACGTTTTAGCCATAAACGGGCATCGAGCAGACGTGAACGCGAAATATAATCGTCCTGAACGGCGGCGAGGTCAGCACCAAAACCTTTATCGTCGATTGCCAGGGTAATCTCGAAATTTAGCCACAGACTACGCATATCAAGGTTAACTGTGCCAACCAGACTTAGTTCGCCATCGACCAGCACGCTCTTGGTATGCAGTAACCCGCCTTCAAACTGATAAATTTTAACCCCAGCAGCCAGCAGTTCCGTAAAGAATGCGCGACTGGC  >  NC_000913/1307215‑1307508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`