BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,325,092	T→C	V116A (GTG→GCG)	yciQ →	enhancer of membrane protein expression; putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,325,092	0	T	C	100.0%	437.3 / NA	116	V116A (GTG→GCG)	yciQ	enhancer of membrane protein expression; putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (51/65); total (51/65)

Only 100 of 116 total aligned reads displayed.

CACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGA  >  NC_000913/1324944‑1325238

cACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCgg                                                                                                                                                  <  4:103149/151‑1 (MQ=255)

 aCGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCggg                                                                                                                                                 >  4:1623763/1‑151 (MQ=255)

 aCGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCggg                                                                                                                                                 >  4:233394/1‑151 (MQ=255)

        ttATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATaa                                                                                                                                           <  2:25888/150‑1 (MQ=255)

        ttATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATaa                                                                                                                                           <  1:209606/150‑1 (MQ=255)

         tATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATaaa                                                                                                                                          <  2:1516168/150‑1 (MQ=255)

          aTCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACTCGTGATGGTATTCCTGAACCCTGGCATCTTGACCGGCATATCGGGTTAATGAGCATTGTCGCGGGCGATAaac                                                                                                                                         >  4:1312244/1‑150 (MQ=255)

          aTCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAaac                                                                                                                                         >  2:831617/1‑150 (MQ=255)

            cGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAaacaac                                                                                                                                      <  2:422725/151‑1 (MQ=255)

              tGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGt                                                                                                                                    >  1:154678/1‑151 (MQ=255)

                       aCGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCt                                                                                                                           <  3:283104/151‑1 (MQ=255)

                         gACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTc                                                                                                                          >  4:603667/1‑150 (MQ=255)

                         gACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCa                                                                                                                         <  2:1166521/151‑1 (MQ=255)

                          aCTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCa                                                                                                                         >  4:1287674/1‑150 (MQ=255)

                            tATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAgg                                                                                                                      >  2:1456073/1‑151 (MQ=255)

                             aTGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAga                                                                                                                      <  1:272015/150‑2 (MQ=255)

                             aTGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAg                                                                                                                       <  3:47091/149‑1 (MQ=255)

                             aTGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAg                                                                                                                       <  2:1335206/149‑1 (MQ=255)

                             aTGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGc                                                                                                                     <  4:376087/151‑1 (MQ=255)

                             aTGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGc                                                                                                                     <  4:1429669/151‑1 (MQ=255)

                               ggATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCg                                                                                                                    <  3:207689/150‑1 (MQ=255)

                               ggATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCg                                                                                                                    >  4:1644199/1‑150 (MQ=255)

                                   gCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTAt                                                                                                               <  2:634935/151‑1 (MQ=255)

                                     aGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATct                                                                                                             <  2:734238/151‑2 (MQ=255)

                                     aGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATc                                                                                                              >  4:1445929/1‑150 (MQ=255)

                                      gCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCAt                                                                                                            <  2:781795/151‑1 (MQ=255)

                                           aTGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCa                                                                                                       <  4:1402058/151‑1 (MQ=255)

                                             ggggACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCaaa                                                                                                     <  4:951432/151‑1 (MQ=255)

                                             ggggACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCaa                                                                                                      <  1:214840/150‑1 (MQ=255)

                                              gggACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAAt                                                                                                    >  3:58543/1‑151 (MQ=255)

                                              gggACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAAt                                                                                                    >  4:293597/1‑151 (MQ=255)

                                               ggACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAAtt                                                                                                   <  3:408600/151‑1 (MQ=255)

                                                gACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTc                                                                                                  <  4:1260073/151‑1 (MQ=255)

                                                 aCGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCa                                                                                                 <  2:1142553/151‑1 (MQ=255)

                                                   gCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCAtt                                                                                               <  2:1064393/151‑1 (MQ=255)

                                                     cacACTGCTGAACTATCATATTGTCGGCGTCACCCGTAATGGTATTCCTGAACCATGGCATATTGATTGGCATATCAGGTTAATGAGTATTGTTGCGGGCGATAAACAACGTTTCTTGCGTCAAGCCGACTATCGTTATCAAATTCCTTa                                                                                              >  1:273038/3‑150 (MQ=255)

                                                      gCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATc                                                                                            <  4:1322889/151‑1 (MQ=255)

                                                       cACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATc                                                                                            >  2:436882/1‑150 (MQ=255)

                                                       cACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATc                                                                                            >  1:429081/1‑150 (MQ=255)

                                                        aCTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAg                                                                                          <  2:811859/151‑1 (MQ=255)

                                                          tgctgAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGt                                                                                        >  4:342416/1‑151 (MQ=255)

                                                            ctgAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGtt                                                                                       >  4:646750/1‑150 (MQ=255)

                                                              gAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTaa                                                                                     <  2:566635/150‑1 (MQ=255)

                                                              gAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTaa                                                                                     <  2:202465/150‑1 (MQ=255)

                                                              gAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTa                                                                                      <  3:238876/149‑1 (MQ=255)

                                                              gAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTa                                                                                      <  4:1067436/149‑1 (MQ=255)

                                                              gAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTa                                                                                      <  1:126860/149‑1 (MQ=255)

                                                              gAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTa                                                                                      <  1:397688/149‑1 (MQ=255)

                                                                aCTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTaaaa                                                                                   >  4:1380697/1‑150 (MQ=255)

                                                                  tATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATg                                                                                >  1:421268/1‑151 (MQ=255)

                                                                     cATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATAGCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCtt                                                                             <  1:11077/151‑1 (MQ=255)

                                                                       tatTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTc                                                                           >  2:468528/1‑151 (MQ=255)

                                                                        atTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTcc                                                                          <  2:578979/151‑1 (MQ=255)

                                                                        atTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTcc                                                                          <  2:232066/151‑1 (MQ=255)

                                                                        atTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTc                                                                           <  3:213838/150‑1 (MQ=255)

                                                                         ttgttgGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCt                                                                         >  4:1000243/1‑151 (MQ=255)

                                                                          tgttgGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCt                                                                         <  2:517904/150‑1 (MQ=255)

                                                                          tgttgGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTg                                                                        >  2:654188/1‑151 (MQ=255)

                                                                          tgttgGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTg                                                                        >  1:414172/1‑151 (MQ=255)

                                                                           gttgGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTGTCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGc                                                                       <  4:1120547/151‑1 (MQ=255)

                                                                            ttgGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCg                                                                      >  4:480538/1‑151 (MQ=255)

                                                                                   cACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAgggg                                                               <  3:354817/151‑1 (MQ=255)

                                                                                          gATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTg                                                        >  3:269268/1‑151 (MQ=255)

                                                                                           aTGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGa                                                       <  2:681969/151‑1 (MQ=255)

                                                                                             ggTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGAt                                                      <  2:86080/150‑1 (MQ=255)

                                                                                             ggTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATc                                                     <  4:1236598/151‑1 (MQ=255)

                                                                                              gTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCt                                                    >  2:828841/1‑151 (MQ=255)

                                                                                                aTTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGc                                                  >  4:1608087/1‑151 (MQ=255)

                                                                                                 ttCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCt                                                 >  2:1160767/1‑151 (MQ=255)

                                                                                                        aCCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTgg                                          >  2:1178261/1‑151 (MQ=255)

                                                                                                         cccTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTgg                                          >  2:285952/1‑150 (MQ=255)

                                                                                                          ccTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGa                                         >  4:330254/1‑150 (MQ=255)

                                                                                                             ggCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAAc                                       <  2:1642197/149‑1 (MQ=255)

                                                                                                             ggCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGt                                     <  2:606315/151‑1 (MQ=255)

                                                                                                                aTCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGAc                                  >  4:618727/1‑151 (MQ=255)

                                                                                                                 tCTTGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGAcc                                 >  4:871223/1‑151 (MQ=255)

                                                                                                                    tGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGAcc                                 <  2:1600780/148‑1 (MQ=255)

                                                                                                                    tGACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGt                              >  3:392372/1‑151 (MQ=255)

                                                                                                                     gACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTa                             <  1:395288/151‑1 (MQ=255)

                                                                                                                     gACTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTa                             <  2:1221950/151‑1 (MQ=255)

                                                                                                                       cTGGCATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAAc                           >  2:30904/1‑151 (MQ=255)

                                                                                                                           cATATCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACt                       <  4:1001444/151‑1 (MQ=255)

                                                                                                                              atCGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGc                    <  4:688181/151‑1 (MQ=255)

                                                                                                                                cGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCg                  >  3:61551/1‑151 (MQ=255)

                                                                                                                                cGGGTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCg                  <  4:437492/151‑1 (MQ=255)

                                                                                                                                 gggTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCg                  >  2:1572328/1‑150 (MQ=255)

                                                                                                                                   gTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGttt               <  2:284199/151‑1 (MQ=255)

                                                                                                                                   gTTAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGttt               >  4:1230297/1‑151 (MQ=255)

                                                                                                                                    ttAATGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTg              >  1:401193/1‑151 (MQ=255)

                                                                                                                                      aaTGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGa             <  4:220802/150‑1 (MQ=255)

                                                                                                                                      aaTGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGa             <  4:1457895/150‑1 (MQ=255)

                                                                                                                                      aaTGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGa             <  4:1267055/150‑1 (MQ=255)

                                                                                                                                       aTGAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGaaa           <  4:1285571/151‑1 (MQ=255)

                                                                                                                                       aTGAGTATTGTCGCGAGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGaaa           <  2:539427/151‑1 (MQ=255)

                                                                                                                                         gAGTATTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAAtt         <  2:602844/151‑1 (MQ=255)

                                                                                                                                             aTTGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTtat      <  4:370174/150‑1 (MQ=255)

                                                                                                                                               tGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATaa    <  2:868459/150‑1 (MQ=255)

                                                                                                                                               tGTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATaa    <  2:83429/150‑1 (MQ=255)

                                                                                                                                                gTCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTtata     >  4:1041493/1‑148 (MQ=255)

                                                                                                                                                 tCGCGGGCGATAAACAACGTTTCTTGCCTCAAGGCGGCTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTCACCGGTAACCACTGGCCGTTTGAACTTtat      <  2:1607033/146‑1 (MQ=255)

CACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGATGGGGACGCTGCACTGCTGAACTATCATATTGTTGGCGTCACCCGTGATGGTATTCCTGAACCCTGGCATCTTGACTGGCATATCGGGTTAATGAGTATTGTCGTGGGCGATAAACAACGTTTCTTGCCTCAAGGCGACTATCATTATCAAATTCATTATCAGGTTAAAAATGCTTTCCTGCGTGAGGGGGATTCTGATCTGCTAATCTGGAACGTGACCGGTAACCACTGGCCGTTTGAAATTTATAAGA  >  NC_000913/1324944‑1325238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`