Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,379,556 T→C L558P (CTG→CCG)  ycjT → putative family 65 glycosyl hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,379,5560TC99.3% 553.6 / NA 147L558P (CTG→CCG) ycjTputative family 65 glycosyl hydrolase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (75/71);  total (76/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

TTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACTGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCACTATTCACG  >  NC_000913/1379409‑1379702
                                                                                                                                                   |                                                                                                                                                  
ttATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATAccgc                                                                                                                                                  <  4:1134469/150‑1 (MQ=255)
ttATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATAccgc                                                                                                                                                  <  4:588379/150‑1 (MQ=255)
 tATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATAccgctg                                                                                                                                                >  4:1505670/1‑151 (MQ=255)
  aTCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATAccgctg                                                                                                                                                <  2:1597082/150‑1 (MQ=255)
    ccATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGat                                                                                                                                             <  4:656785/151‑1 (MQ=255)
      aTCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGatta                                                                                                                                           <  4:1598477/151‑1 (MQ=255)
      aTCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGatta                                                                                                                                           <  4:337161/151‑1 (MQ=255)
        cgcgCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGattatt                                                                                                                                         <  3:259732/151‑1 (MQ=255)
        cgcgCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGattat                                                                                                                                          <  2:993526/150‑1 (MQ=255)
             cGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCAcgc                                                                                                                                    <  2:1121601/151‑1 (MQ=255)
              gAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCAcgcg                                                                                                                                   >  2:909167/1‑151 (MQ=255)
              gAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCAcgcg                                                                                                                                   <  4:1492859/151‑1 (MQ=255)
                aaTGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCa                                                                                                                                 <  4:752554/151‑1 (MQ=255)
                  tGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACgcgc                                                                                                                                  >  1:278882/1‑148 (MQ=255)
                   gTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGa                                                                                                                               <  2:1682710/150‑1 (MQ=255)
                    ttCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGa                                                                                                                               >  4:499350/1‑149 (MQ=255)
                     tCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGt                                                                                                                            >  3:469261/1‑151 (MQ=255)
                      ccTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGt                                                                                                                            <  2:224513/150‑1 (MQ=255)
                          aaaGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACg                                                                                                                       >  4:619238/1‑151 (MQ=255)
                             gagCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACg                                                                                                                       >  4:1273939/1‑148 (MQ=255)
                              agCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGAt                                                                                                                   <  4:597178/151‑1 (MQ=255)
                                cTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGc                                                                                                                 >  4:1538099/1‑151 (MQ=255)
                                cTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGc                                                                                                                 >  1:449201/1‑151 (MQ=255)
                                 tATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGc                                                                                                                 >  4:957449/1‑150 (MQ=255)
                                    ggATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGAt                                                                                                             <  2:1599656/151‑1 (MQ=255)
                                     gATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGc                                                                                                                 >  2:906591/1‑146 (MQ=255)
                                       tGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATcc                                                                                                           >  2:311407/1‑150 (MQ=255)
                                        gCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCt                                                                                                          <  4:1368207/150‑1 (MQ=255)
                                          cAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAGATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCaa                                                                                                       <  4:10623/151‑1 (MQ=255)
                                          cAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTc                                                                                                         >  4:1631436/1‑149 (MQ=255)
                                                  cAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTg                                                                                               >  2:823401/1‑151 (MQ=255)
                                                   aGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGa                                                                                              <  3:278898/151‑1 (MQ=255)
                                                      ccGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGAtgt                                                                                           <  2:259823/151‑1 (MQ=255)
                                                      ccGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGAtg                                                                                            >  2:576029/1‑150 (MQ=255)
                                                        gACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATgtgg                                                                                         >  2:1375638/1‑151 (MQ=255)
                                                            gCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGAt                                                                                     <  2:1272984/151‑1 (MQ=255)
                                                             cGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATg                                                                                    >  3:467254/1‑151 (MQ=255)
                                                                tttGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTc                                                                                 >  3:221117/1‑151 (MQ=255)
                                                                   gCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCaa                                                                               <  2:897009/150‑1 (MQ=255)
                                                                        aGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACAt                                                                         >  3:335768/1‑151 (MQ=255)
                                                                        aGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACAt                                                                         <  4:426008/151‑1 (MQ=255)
                                                                        aGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACAt                                                                         <  2:1526806/151‑1 (MQ=255)
                                                                         ggATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACAtg                                                                        >  2:115268/1‑151 (MQ=255)
                                                                             gatTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACAtgctgc                                                                    <  1:292522/151‑1 (MQ=255)
                                                                             gatTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACAtgctg                                                                     <  4:256275/150‑1 (MQ=255)
                                                                                tCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCgg                                                                 >  3:222965/1‑151 (MQ=255)
                                                                                  gTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGa                                                                <  2:599296/150‑1 (MQ=255)
                                                                                    ttATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGc                                                              <  2:268631/150‑1 (MQ=255)
                                                                                     tATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGc                                                              >  1:217409/1‑149 (MQ=255)
                                                                                      aTGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAg                                                            <  2:1355441/150‑1 (MQ=255)
                                                                                       tGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGtt                                                          >  2:1552278/1‑151 (MQ=255)
                                                                                        ggCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTtc                                                         <  1:467234/151‑1 (MQ=255)
                                                                                            aaGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTtctc                                                       >  2:67668/1‑149 (MQ=255)
                                                                                            aaGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCa                                                      <  3:104770/150‑1 (MQ=255)
                                                                                            aaGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAg                                                     <  2:375673/151‑1 (MQ=255)
                                                                                             aGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGc                                                    >  2:1064047/1‑151 (MQ=255)
                                                                                                cGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGc                                                 <  2:706638/151‑1 (MQ=255)
                                                                                                 ggCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGc                                                 >  4:1509102/1‑150 (MQ=255)
                                                                                                  gCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCAt                                               <  2:516197/151‑1 (MQ=255)
                                                                                                  gCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCAt                                               >  3:370425/1‑151 (MQ=255)
                                                                                                    taTTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATc                                              >  4:328270/2‑150 (MQ=255)
                                                                                                       tAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCgtgt                                          >  3:354226/1‑151 (MQ=255)
                                                                                                       tAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCgtg                                           <  2:784580/150‑1 (MQ=255)
                                                                                                        aaTCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCgtgt                                          >  2:976/1‑150 (MQ=255)
                                                                                                        aaTCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCgtgt                                          <  2:846124/150‑1 (MQ=255)
                                                                                                         aTCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCt                                        >  2:187608/1‑151 (MQ=255)
                                                                                                          tCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCtt                                       >  4:386471/1‑151 (MQ=255)
                                                                                                            tGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGc                                     >  2:1003665/1‑151 (MQ=255)
                                                                                                             ggCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCtt                                       <  2:927033/148‑1 (MQ=255)
                                                                                                             ggCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCtt                                       <  2:25547/148‑1 (MQ=255)
                                                                                                             ggCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCt                                        <  4:1439608/147‑1 (MQ=255)
                                                                                                             ggCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCt                                        <  3:349722/147‑1 (MQ=255)
                                                                                                              gCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGcc                                    >  4:500385/1‑150 (MQ=255)
                                                                                                                gAAATACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAAt                                 <  1:308215/151‑1 (MQ=255)
                                                                                                                 aaaTACAAAGCGGCGGCGGGGAAGTAAACCATTCTGCTGGATATTTCAAGCGCAGAAGTGGAGGAGAAGCAGATCATCAAAAAAGCTGATGTGGGGAAGGTCAATTATAAGCTGCCGGACCAGATCTAAGCGGCAGCGAGTCTTGCCAAt                                 >  2:1110571/1‑150 (MQ=255)
                                                                                                                 aaaTACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATc                                >  4:928171/1‑151 (MQ=255)
                                                                                                                  aaTACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCt                               <  2:1503793/151‑1 (MQ=255)
                                                                                                                  aaTACAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCt                               >  2:740355/1‑151 (MQ=255)
                                                                                                                     aCAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGc                             <  4:984722/150‑1 (MQ=255)
                                                                                                                     aCAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGc                             >  1:182743/1‑150 (MQ=255)
                                                                                                                     aCAAAGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGc                             >  3:164399/1‑150 (MQ=255)
                                                                                                                       aaaGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAAt                          <  4:398625/151‑1 (MQ=255)
                                                                                                                       aaaGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAAt                          >  4:1364113/1‑151 (MQ=255)
                                                                                                                        aaGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAAtt                         >  2:65772/1‑151 (MQ=255)
                                                                                                                         aGCGGCGGCGGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAAttt                        >  4:252154/1‑151 (MQ=255)
                                                                                                                          gcggcggcgGGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAAtttt                       >  1:265058/1‑151 (MQ=255)
                                                                                                                           cggcggcggGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTa                      <  4:359011/151‑1 (MQ=255)
                                                                                                                            ggcggcggGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTAt                     >  2:519136/1‑151 (MQ=255)
                                                                                                                              cggcggGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTAt                     >  1:72190/1‑149 (MQ=255)
                                                                                                                               ggcggGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGaa                  <  2:385909/151‑1 (MQ=255)
                                                                                                                               ggcggGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGa                   >  2:239710/1‑150 (MQ=255)
                                                                                                                                gcggGGAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAAc                 <  4:120598/151‑1 (MQ=255)
                                                                                                                                  ggggAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACcg               >  4:276954/1‑151 (MQ=255)
                                                                                                                                   gggAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACcgc              >  4:327654/1‑151 (MQ=255)
                                                                                                                                    ggAAGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACcgcg             <  3:165738/151‑1 (MQ=255)
                                                                                                                                      aaGCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCa           <  2:1316460/151‑1 (MQ=255)
                                                                                                                                        gCAAACCATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCACt         <  2:713582/151‑1 (MQ=255)
                                                                                                                                              cATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCACTATTCAc   <  4:804532/151‑1 (MQ=255)
                                                                                                                                              cATACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCACTATTCAc   <  4:1580655/151‑1 (MQ=255)
                                                                                                                                               aTACCGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCACTATTCACg  <  2:1552464/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAAATACAAAGCGGCGGCGGGGAAGCAAACCATACTGCTGGATTATTCACGCGCAGAAGTGAACGAGATGCAGATCCTCAAACAAGCTGATGTGGTGATGCTCAATTACATGCTGCCGGAGCAGTTCTCAGCGGCATCGTGTCTTGCCAATCTGCAATTTTATGAACCGCGCACTATTCACG  >  NC_000913/1379409‑1379702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: