Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,399,186 T→C Q114R (CAG→CGG)  fnr ← oxygen‑sensing anaerobic growth regulon transcriptional regulator FNR; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,399,1860TC98.2% 385.6 / ‑1.3 109Q114R (CAG→CGG) fnroxygen‑sensing anaerobic growth regulon transcriptional regulator FNR; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (57/50);  total (59/50)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 110 total aligned reads displayed.

TCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAA  >  NC_000913/1399037‑1399333
                                                                                                                                                     |                                                                                                                                                   
tcttcGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTg                                                                                                                                                    >  1:373513/1‑151 (MQ=255)
   tcGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCgc                                                                                                                                                   <  4:737515/149‑1 (MQ=255)
    cGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCgcgc                                                                                                                                                 <  4:239652/150‑1 (MQ=255)
    cGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCgcgc                                                                                                                                                 <  2:923467/150‑1 (MQ=255)
     gACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCcgcgcg                                                                                                                                                >  2:1611446/1‑150 (MQ=255)
       caacaGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAg                                                                                                                                             <  3:464030/151‑1 (MQ=255)
           agcagGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCg                                                                                                                                         <  4:1414197/151‑1 (MQ=255)
            gcagGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCgg                                                                                                                                        <  4:1620676/151‑1 (MQ=255)
              agGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGt                                                                                                                                      >  4:508779/1‑151 (MQ=255)
              agGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGt                                                                                                                                      <  2:883168/151‑1 (MQ=255)
                gATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTg                                                                                                                                     >  4:1605213/1‑150 (MQ=255)
                  tCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATg                                                                                                                                  >  4:780223/1‑151 (MQ=255)
                      gTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCg                                                                                                                              >  2:919079/1‑151 (MQ=255)
                      gTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCg                                                                                                                              >  2:919102/1‑151 (MQ=255)
                        ccTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGc                                                                                                                             <  4:166095/150‑1 (MQ=255)
                         cTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTg                                                                                                                           >  4:627377/1‑151 (MQ=255)
                           gATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGc                                                                                                                          <  2:1070409/150‑1 (MQ=255)
                           gATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGc                                                                                                                          <  4:1220217/150‑1 (MQ=255)
                            aTCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCg                                                                                                                        >  4:226244/1‑151 (MQ=255)
                            aTCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCg                                                                                                                        >  2:683883/1‑151 (MQ=255)
                             tCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCg                                                                                                                        >  4:1319242/1‑150 (MQ=255)
                                   ttGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCg                                                                                                                  <  2:1359826/150‑1 (MQ=255)
                                    tGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGt                                                                                                                 <  3:80688/150‑1 (MQ=255)
                                    tGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTc                                                                                                                >  2:674510/1‑151 (MQ=255)
                                    tGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTc                                                                                                                >  2:1491042/1‑151 (MQ=255)
                                    tGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTc                                                                                                                >  2:1575939/1‑151 (MQ=255)
                                     gATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTc                                                                                                                >  4:1209401/1‑150 (MQ=255)
                                     gATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTc                                                                                                                <  3:98720/150‑1 (MQ=255)
                                     gATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTc                                                                                                                >  1:219544/1‑150 (MQ=255)
                                       tttCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCaa                                                                                                              >  4:1088536/1‑150 (MQ=255)
                                         tCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAAt                                                                                                            <  2:582117/150‑1 (MQ=255)
                                           aCCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATccc                                                                                                         <  4:377833/151‑1 (MQ=255)
                                              gCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCccacc                                                                                                      >  2:1601006/1‑151 (MQ=255)
                                                tcatcaGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCccacc                                                                                                      >  2:416692/1‑149 (MQ=255)
                                                 catcaGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAgg                                                                                                   >  1:448701/1‑151 (MQ=255)
                                                    caGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTc                                                                                                 <  4:46818/150‑1 (MQ=255)
                                                       aCGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGcc                                                                                              >  1:359487/1‑150 (MQ=255)
                                                          catcatCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCt                                                                                          >  2:402447/1‑151 (MQ=255)
                                                          catcatCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCt                                                                                          <  4:83792/151‑1 (MQ=255)
                                                          catcatCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCt                                                                                          <  1:70190/151‑1 (MQ=255)
                                                          catcatCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCt                                                                                          <  2:179258/151‑1 (MQ=255)
                                                                  gctgACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAAt                                                                                      <  2:149089/147‑1 (MQ=255)
                                                                     gACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGaaa                                                                                 <  4:839249/149‑1 (MQ=255)
                                                                     gACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAAc                                                                                <  4:1416524/150‑1 (MQ=255)
                                                                      aCGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAAcc                                                                               >  3:397829/1‑150 (MQ=255)
                                                                      aCGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCa                                                                              <  4:198562/151‑1 (MQ=255)
                                                                         cAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTg                                                                           >  4:1247729/1‑151 (MQ=255)
                                                                           gATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGAt                                                                         >  2:485544/1‑151 (MQ=255)
                                                                            aTTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGAtt                                                                        >  4:757180/1‑151 (MQ=255)
                                                                               cGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGc                                                                     >  2:976447/1‑151 (MQ=255)
                                                                               cGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGc                                                                     >  4:433752/1‑151 (MQ=255)
                                                                                ggCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCt                                                                    <  2:1657982/151‑1 (MQ=255)
                                                                                  cATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCtcg                                                                  >  2:1060423/1‑151 (MQ=255)
                                                                                     tttACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCtcgtc                                                                <  2:1271537/150‑1 (MQ=255)
                                                                                     tttACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCtcgtc                                                                <  4:515866/150‑1 (MQ=255)
                                                                                      ttACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCtcgtcg                                                               <  2:1136753/150‑1 (MQ=255)
                                                                                       tACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGcc                                                             >  3:358127/1‑151 (MQ=255)
                                                                                         ccGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCt                                                            <  4:31984/150‑1 (MQ=255)
                                                                                           ggACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGc                                                         >  2:862185/1‑151 (MQ=255)
                                                                                             aCAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTc                                                       <  2:508074/151‑1 (MQ=255)
                                                                                               aaaTCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTc                                                       >  2:1564554/1‑149 (MQ=255)
                                                                                                 aTCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTg                                                   >  4:26719/1‑151 (MQ=255)
                                                                                                 aTCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTg                                                   <  2:395070/151‑1 (MQ=255)
                                                                                                  tCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTg                                                   >  4:52969/1‑150 (MQ=255)
                                                                                                   cGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGAt                                                 <  1:187030/151‑1 (MQ=255)
                                                                                                    gTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATg                                                <  2:1147143/151‑1 (MQ=255)
                                                                                                     tCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATgg                                               >  2:79522/1‑151 (MQ=255)
                                                                                                      ccAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATgg                                               >  4:612646/1‑150 (MQ=255)
                                                                                                      ccAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGt                                              <  4:1032798/151‑1 (MQ=255)
                                                                                                       cAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATgg                                               >  3:447275/1‑149 (MQ=255)
                                                                                                       cAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGt                                              >  2:1528228/1‑150 (MQ=255)
                                                                                                        aGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGTAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGtat                                            <  4:1261514/151‑1 (MQ=255)
                                                                                                         gCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGtat                                            >  2:1558004/1‑150 (MQ=255)
                                                                                                             ttCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACtt                                       >  2:1103221/1‑151 (MQ=255)
                                                                                                               cGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACtttt                                     >  1:204480/1‑151 (MQ=255)
                                                                                                                 aaCGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTa                                    >  1:41309/1‑150 (MQ=255)
                                                                                                                  aCGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAAt                                  <  3:414721/151‑1 (MQ=255)
                                                                                                                   cGGGATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAAt                                  <  4:782831/150‑1 (MQ=255)
                                                                                                                    gggATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCg                                >  4:412674/1‑151 (MQ=255)
                                                                                                                     ggATTTCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGt                               <  3:15309/151‑1 (MQ=255)
                                                                                                                          tCACATACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCg                           <  4:65002/150‑1 (MQ=255)
                                                                                                                           cacaTACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCgg                          >  2:93023/1‑150 (MQ=255)
                                                                                                                           cacaTACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGa                         <  1:127053/151‑1 (MQ=255)
                                                                                                                              aTACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCg                      >  2:1302130/1‑151 (MQ=255)
                                                                                                                              aTACCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCg                      <  4:1150633/151‑1 (MQ=255)
                                                                                                                                aCCATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGa                    <  4:236709/151‑1 (MQ=255)
                                                                                                                                 ccATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGa                    <  4:532045/150‑1 (MQ=255)
                                                                                                                                 ccATCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGAt                   >  4:117209/1‑151 (MQ=255)
                                                                                                                                   aTCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATgg                 <  3:275905/151‑1 (MQ=255)
                                                                                                                                    tCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGc                >  1:37994/1‑151 (MQ=255)
                                                                                                                                    tCGAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGc                >  1:266245/1‑151 (MQ=255)
                                                                                                                                      gAGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCAt              <  2:587841/151‑1 (MQ=255)
                                                                                                                                       aGGTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCAt              >  2:53715/1‑150 (MQ=255)
                                                                                                                                        ggTTTCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATa             >  4:133917/1‑150 (MQ=255)
                                                                                                                                            tCCAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGc         <  2:327125/150‑1 (MQ=255)
                                                                                                                                             ccAGCGCCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCg        <  2:1167764/150‑1 (MQ=255)
                                                                                                                                                 cgcCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGAttt    >  2:44482/1‑150 (MQ=255)
                                                                                                                                                 cgcCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTa   <  3:392254/151‑1 (MQ=255)
                                                                                                                                                  gcCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTaa  <  4:1525563/151‑1 (MQ=255)
                                                                                                                                                  gcCCGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTa   >  4:760279/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAA  >  NC_000913/1399037‑1399333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑