Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,476,832 T→C Y563Y (TAT→TAC)  ydbD → PF10971 family putative periplasmic methylglyoxal resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,476,8320TC100.0% 500.9 / NA 132Y563Y (TAT→TAC) ydbDPF10971 family putative periplasmic methylglyoxal resistance protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (67/65);  total (67/65)

Only 100 of 133 total aligned reads displayed.

ACAACGGTTATTGACACGTTTCAACGATCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCT  >  NC_000913/1476685‑1476979
                                                                                                                                                   |                                                                                                                                                   
aCAACGGTTATTGACACGTTTCAACGATCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGaa                                                                                                                                                  <  4:261924/151‑1 (MQ=255)
  aaCGGTTATTGACACGTTTCAACGATCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAAc                                                                                                                                                >  2:1156095/1‑151 (MQ=255)
    cGGTTATTGACACGTTTCAACGATCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAAcc                                                                                                                                               >  4:450518/1‑150 (MQ=255)
       ttATTGACACGTTTCAACGATCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAgcg                                                                                                                                           >  4:1128775/1‑151 (MQ=255)
         aTTGACACGTTTCAACGATCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCt                                                                                                                                         >  2:292053/1‑151 (MQ=255)
         aTTGACACGTTTCAACGATCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCt                                                                                                                                         <  3:58715/151‑1 (MQ=255)
           tGACACGTTTCAACGATCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTg                                                                                                                                        <  2:29523/150‑1 (MQ=255)
           tGACACGTTTCAACGATCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTg                                                                                                                                        <  4:114655/150‑1 (MQ=255)
            gACACGTTTCAACGATCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGat                                                                                                                                      <  4:369675/151‑1 (MQ=255)
               acGTTTCAACGATCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATg                                                                                                                                   <  4:98466/151‑1 (MQ=255)
                   ttCAACGATCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAGAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGAtt                                                                                                                                <  1:43625/150‑1 (MQ=255)
                   ttCAACGATCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGAtt                                                                                                                                <  4:1648215/150‑1 (MQ=255)
                    tCAACGATCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGAtttt                                                                                                                              >  4:311899/1‑151 (MQ=255)
                      aaCGATCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGt                                                                                                                            >  4:1405944/1‑151 (MQ=255)
                       aCGATCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTc                                                                                                                           >  2:769303/1‑151 (MQ=255)
                        cGATCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCt                                                                                                                          <  2:537192/151‑1 (MQ=255)
                          aTCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGa                                                                                                                        <  4:1348147/151‑1 (MQ=255)
                           tCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTg                                                                                                                         >  4:1260649/1‑149 (MQ=255)
                           tCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTg                                                                                                                         >  2:990676/1‑149 (MQ=255)
                               gAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCt                                                                                                                     <  1:318362/149‑1 (MQ=255)
                               gAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTg                                                                                                                    <  4:1139671/150‑1 (MQ=255)
                               gAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTg                                                                                                                    >  2:230995/1‑150 (MQ=255)
                               gAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTg                                                                                                                    >  2:940773/1‑150 (MQ=255)
                               gAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTg                                                                                                                    >  4:1404004/1‑150 (MQ=255)
                                aaGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGAt                                                                                                                  >  2:922837/1‑151 (MQ=255)
                                  gggTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTa                                                                                                                <  2:1473428/151‑1 (MQ=255)
                                  gggTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTa                                                                                                                <  2:1137654/151‑1 (MQ=255)
                                      ggAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGcc                                                                                                             <  4:609245/150‑1 (MQ=255)
                                          tAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAACCAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACgg                                                                                                         >  4:1133657/1‑150 (MQ=255)
                                           aaTCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGc                                                                                                        <  2:1591744/150‑1 (MQ=255)
                                               tGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGt                                                                                                   >  2:433513/1‑151 (MQ=255)
                                                 gCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTtg                                                                                                 <  4:280129/151‑1 (MQ=255)
                                                   gAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTtgat                                                                                               >  2:1414590/1‑151 (MQ=255)
                                                     aaaaTCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTtgatga                                                                                             <  2:774885/151‑1 (MQ=255)
                                                     aaaaTCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTtgatg                                                                                              >  3:134313/1‑150 (MQ=255)
                                                      aaaTCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGAc                                                                                            <  4:758452/151‑1 (MQ=255)
                                                         tCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGAcc                                                                                           >  2:286799/1‑149 (MQ=255)
                                                         tCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGAcc                                                                                           >  4:1278313/1‑149 (MQ=255)
                                                          cAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGa                                                                                        <  1:385604/151‑1 (MQ=255)
                                                            gTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATg                                                                                      >  3:23340/1‑151 (MQ=255)
                                                                  gAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCt                                                                                    <  4:1518230/147‑1 (MQ=255)
                                                                  gAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTAt                                                                                  <  2:1333123/149‑1 (MQ=255)
                                                                  gAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTa                                                                                <  2:628502/151‑1 (MQ=255)
                                                                     cACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAAt                                                                             >  2:103810/1‑151 (MQ=255)
                                                                      aCGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATg                                                                            <  4:223429/151‑1 (MQ=255)
                                                                       cGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGc                                                                           >  2:1221706/1‑151 (MQ=255)
                                                                       cGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGc                                                                           >  4:1483602/1‑151 (MQ=255)
                                                                          cGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCAc                                                                        >  4:717424/1‑151 (MQ=255)
                                                                          cGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCAc                                                                        >  1:175636/1‑151 (MQ=255)
                                                                          cGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCAc                                                                        >  2:1114252/1‑151 (MQ=255)
                                                                               aaaCAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGccc                                                                   <  3:175650/151‑1 (MQ=255)
                                                                                aaCAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGccc                                                                   >  4:473377/1‑150 (MQ=255)
                                                                                aaCAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCt                                                                  <  2:142677/151‑1 (MQ=255)
                                                                                  cAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTg                                                                 >  3:54895/1‑150 (MQ=255)
                                                                                    aaaaaTTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGAt                                                               >  4:667700/1‑150 (MQ=255)
                                                                                     aaaaTTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATAc                                                             <  2:813852/151‑1 (MQ=255)
                                                                                      aaaTTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACg                                                            <  2:368106/151‑1 (MQ=255)
                                                                                          ttGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTc                                                         <  1:199423/150‑1 (MQ=255)
                                                                                           tGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCgg                                                       >  2:724544/1‑151 (MQ=255)
                                                                                           tGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCg                                                        <  4:35512/150‑1 (MQ=255)
                                                                                            ggCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCgg                                                       <  3:39763/150‑1 (MQ=255)
                                                                                             gCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGgac                                                     <  1:310853/151‑1 (MQ=255)
                                                                                              cAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGgacg                                                    <  2:709154/151‑1 (MQ=255)
                                                                                              cAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGgacg                                                    <  4:1601684/151‑1 (MQ=255)
                                                                                                aCGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGgacgac                                                  >  1:185263/1‑151 (MQ=255)
                                                                                                  gTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTg                                                <  4:1010476/151‑1 (MQ=255)
                                                                                                  gTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTg                                                >  2:392164/1‑151 (MQ=255)
                                                                                                    gCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTgg                                               <  2:1565450/150‑1 (MQ=255)
                                                                                                    gCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTgg                                               <  2:1259063/150‑1 (MQ=255)
                                                                                                     cTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTggg                                              >  4:320266/1‑150 (MQ=255)
                                                                                                      tttATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGAc                                            >  4:830566/1‑151 (MQ=255)
                                                                                                        tATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACt                                           <  2:371566/150‑1 (MQ=255)
                                                                                                        tATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACt                                           <  4:559906/150‑1 (MQ=255)
                                                                                                         aTCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACtt                                          <  4:1485818/150‑1 (MQ=255)
                                                                                                           cGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACttttt                                       >  2:1500249/1‑151 (MQ=255)
                                                                                                             cATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACttttt                                       >  2:1175804/1‑149 (MQ=255)
                                                                                                             cATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTag                                     <  3:185277/151‑1 (MQ=255)
                                                                                                              aTCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACttttt                                       >  1:118652/1‑148 (MQ=255)
                                                                                                               tCGGAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTag                                     >  2:558579/1‑149 (MQ=255)
                                                                                                                 ggAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTaga                                    >  3:337417/1‑148 (MQ=255)
                                                                                                                  gAAAAATAATCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGt                                <  4:1636894/151‑1 (MQ=255)
                                                                                                                      aataatCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCat                            <  2:449878/151‑1 (MQ=255)
                                                                                                                      aataatCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCat                            <  2:1526778/151‑1 (MQ=255)
                                                                                                                       ataatCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCatt                           <  2:429214/151‑1 (MQ=255)
                                                                                                                         aatCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCatta                          >  4:772344/1‑150 (MQ=255)
                                                                                                                          atCCGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCattaat                        >  4:1026449/1‑151 (MQ=255)
                                                                                                                             cGAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAg                     <  4:941994/151‑1 (MQ=255)
                                                                                                                              gAAAGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGt                    >  3:230367/1‑151 (MQ=255)
                                                                                                                               aaaGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGtt                   <  4:1249664/151‑1 (MQ=255)
                                                                                                                               aaaGCAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGtt                   >  4:1047386/1‑151 (MQ=255)
                                                                                                                                   cAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTggg                >  4:179333/1‑150 (MQ=255)
                                                                                                                                   cAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGa               <  4:1157561/151‑1 (MQ=255)
                                                                                                                                   cAGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGa               <  4:770329/151‑1 (MQ=255)
                                                                                                                                    aGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGAt              >  4:1102584/1‑151 (MQ=255)
                                                                                                                                    aGATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGAt              <  3:428148/151‑1 (MQ=255)
                                                                                                                                     gATTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGAt              >  2:1209317/1‑150 (MQ=255)
                                                                                                                                      aTTCAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATa             >  3:212621/1‑150 (MQ=255)
                                                                                                                                         cAGATGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAAttt         >  4:1067775/1‑151 (MQ=255)
                                                                                                                                             tGCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTAccc     >  4:379949/1‑151 (MQ=255)
                                                                                                                                              gCATACGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCg    >  2:71600/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ACAACGGTTATTGACACGTTTCAACGATCCTGAAGGGTGGAGTAATCTGGCGAAAAATCAGTATCTGAGCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTATTAAATGCCACCGCCCTGATACGTTCGGACGACTGGGACTTTTTAGAGAGTGCATTAATTAGTTGGGATAATTTACCCGCT  >  NC_000913/1476685‑1476979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑