BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,541,618	C→T	D325N (GAC→AAC)	narZ ←	nitrate reductase 2 (NRZ), alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,541,618	0	C	T	100.0%	406.8 / NA	110	D325N (GAC→AAC)	narZ	nitrate reductase 2 (NRZ), alpha subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (54/56); total (54/56)

Only 100 of 110 total aligned reads displayed.

TGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTAATGGCGATGGTTTT  >  NC_000913/1541473‑1541762

tGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTgg                                                                                                                                             >  4:123839/1‑151 (MQ=255)

 gCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCTGTCGGTGCTAAAGTAGCTGCTggg                                                                                                                                            >  2:603177/1‑151 (MQ=255)

 gCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGGTAAAGTAGTTGCTggg                                                                                                                                            >  4:465765/1‑151 (MQ=255)

  cGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGa                                                                                                                                           <  2:1556539/151‑1 (MQ=255)

    gATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGAtt                                                                                                                                         >  2:591634/1‑151 (MQ=255)

       tgttgCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTAt                                                                                                                                       >  4:1406016/1‑150 (MQ=255)

       tgttgCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATc                                                                                                                                      >  1:360185/1‑151 (MQ=255)

         ttgCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCg                                                                                                                                     >  3:153266/1‑150 (MQ=255)

          tgCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCga                                                                                                                                    <  2:418976/150‑1 (MQ=255)

           gCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCgag                                                                                                                                   <  2:603115/150‑1 (MQ=255)

           gCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCg                                                                                                                                     >  2:651349/1‑148 (MQ=255)

            cTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCg                                                                                                                                     >  3:439011/1‑147 (MQ=255)

              ttCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGAt                                                                                                                                 <  2:590028/149‑1 (MQ=255)

              ttCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATg                                                                                                                                <  2:1221230/150‑1 (MQ=255)

                cGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGaa                                                                                                                              <  4:145618/150‑1 (MQ=255)

                cGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGaa                                                                                                                              <  4:1046080/150‑1 (MQ=255)

                   ccAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGAAGTTGCTGGGGTTATCGAGATGAAActc                                                                                                                          >  2:1208334/1‑151 (MQ=255)

                    cAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAActct                                                                                                                         >  4:165722/1‑151 (MQ=255)

                    cAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAActct                                                                                                                         <  2:209911/151‑1 (MQ=255)

                     aGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCtt                                                                                                                        <  2:1146369/151‑1 (MQ=255)

                        ccatccaCCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTa                                                                                                                     <  4:397418/151‑1 (MQ=255)

                         catccaCCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTa                                                                                                                     >  3:364899/1‑150 (MQ=255)

                          atccaCCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCCGTAGGTGATAAAGAAGTTGCTGGGGTTATCGAGATGAAACTCTTTTaa                                                                                                                    >  2:892925/1‑150 (MQ=255)

                           tccaCCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGGAGTTGCTGGGATTATCGAGATGAAACTCTTTTaaa                                                                                                                   >  2:1682356/1‑150 (MQ=255)

                            ccaccaGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTaaaa                                                                                                                  <  3:450990/150‑1 (MQ=255)

                            ccaccaGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTaaaa                                                                                                                  >  4:1136224/1‑150 (MQ=255)

                            ccaccaGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTaaaa                                                                                                                  <  3:178464/150‑1 (MQ=255)

                            ccaccaGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAAt                                                                                                                 <  4:1224555/151‑1 (MQ=255)

                              accaGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATca                                                                                                               <  3:161259/151‑1 (MQ=255)

                                caGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACAATCGTCGCGAGGCTCCAGAATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGGAGTTGATAAAGCAGGTTCTGGGATTCTCATGTAGAAAATCCTTTAAAAacac                                                                                                              >  2:368388/1‑150 (MQ=255)

                                  ggTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATg                                                                                                           <  4:494458/151‑1 (MQ=255)

                                      aGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGccc                                                                                                       <  3:178533/151‑1 (MQ=255)

                                        aTGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCAt                                                                                                     >  4:198220/1‑151 (MQ=255)

                                         tGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTTCAGCATTACCAGCATCGGCATGTCGCTGTAGCGACGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCAt                                                                                                     <  1:359962/150‑1 (MQ=255)

                                          gcgcGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCAtt                                                                                                    <  2:1464097/150‑1 (MQ=255)

                                          gcgcGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCAtt                                                                                                    <  4:1556005/150‑1 (MQ=255)

                                          gcgcGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTg                                                                                                   <  3:397549/151‑1 (MQ=255)

                                            gcgGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGcc                                                                                                 >  2:58753/1‑151 (MQ=255)

                                            gcgGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGcc                                                                                                 <  4:234829/151‑1 (MQ=255)

                                               gATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTTAAATCACATGGCCCATTGCCAt                                                                                               >  2:1255286/1‑150 (MQ=255)

                                                aTCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCg                                                                                             <  2:767511/151‑1 (MQ=255)

                                                aTCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCg                                                                                             <  3:50563/151‑1 (MQ=255)

                                                  cATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGcc                                                                                           >  2:1036938/1‑151 (MQ=255)

                                                    tGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGGAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGcc                                                                                           >  4:1198908/1‑149 (MQ=255)

                                                     gcgcCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAgg                                                                                        >  4:216567/1‑151 (MQ=255)

                                                     gcgcCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAgg                                                                                        <  1:446950/151‑1 (MQ=255)

                                                        cccGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAGCTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGgc                                                                                      <  2:223444/150‑1 (MQ=255)

                                                         ccGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGgcg                                                                                     >  4:1122911/1‑150 (MQ=255)

                                                             gAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTAt                                                                                 >  1:353436/1‑150 (MQ=255)

                                                                         cATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGttt                                                                    >  3:121807/1‑151 (MQ=255)

                                                                          aTCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGttt                                                                    >  1:175767/1‑150 (MQ=255)

                                                                           tcgtcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCg                                                                  >  2:656281/1‑151 (MQ=255)

                                                                            cgtcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCg                                                                  <  3:474392/150‑1 (MQ=255)

                                                                            cgtcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCg                                                                  <  2:389329/150‑1 (MQ=255)

                                                                             gtcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCgg                                                                 >  3:50278/1‑150 (MQ=255)

                                                                             gtcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGt                                                                <  2:1470641/151‑1 (MQ=255)

                                                                              tcgCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTg                                                               >  1:321829/1‑151 (MQ=255)

                                                                                gcgAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTgcc                                                             >  2:823828/1‑151 (MQ=255)

                                                                                 tgAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTgcc                                                             >  2:481805/2‑150 (MQ=255)

                                                                                   aGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTgccagc                                                          <  1:50274/151‑1 (MQ=255)

                                                                                    ggCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTgccagcc                                                         <  2:165444/151‑1 (MQ=255)

                                                                                       tCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCCGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCATTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGGGGCAGCCACt                                                      >  2:1420956/1‑151 (MQ=255)

                                                                                           gCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTc                                                  <  4:592455/151‑1 (MQ=255)

                                                                                            cATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGGTGAAACTCTTTTAAAATCACATGGCCCATTGCCCTCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTgg                                                    >  2:755454/1‑148 (MQ=255)

                                                                                             aTTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGc                                                <  2:1197387/151‑1 (MQ=255)

                                                                                                aCCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGGAGTTGCTGGGATTATCGAGATGAAACTCTTTTTAAATCACATGGCCCCTTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGca                                               >  2:937521/1‑149 (MQ=255)

                                                                                                  cAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAAt                                           <  3:441588/151‑1 (MQ=255)

                                                                                                   aGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAAtt                                          <  4:163580/151‑1 (MQ=255)

                                                                                                   aGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGGTGATAAAGTAGGTGCTGGGATTATCGAGATGAAACTCTTTTTAAATCACATGGCCCCATGCCATCCCCAGGGCGCTATCAGTGGCTTGTTTCGGTGGCAGCCACTGGTCGCACAAtt                                          >  2:1428504/1‑151 (MQ=255)

                                                                                                      aTCGGCATGTCGCTGTAGCGGCGGCAGGAGGTGGTAAAGTAGTTGCTGGGGTTATCGAGGTGAAAATCTTTTTAAAACACATGGCCCCTTGCCATCGCCCGGGCGCTATCAGTGCCTTTTTTTGGTGGCAGCCCCTGGGCGCACAAttttg                                       >  4:188335/1‑149 (MQ=255)

                                                                                                       tCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGc                                      >  2:1484829/1‑151 (MQ=255)

                                                                                                        cGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGcc                                     <  3:333829/151‑1 (MQ=255)

                                                                                                          gCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCAc                                   <  4:1144832/151‑1 (MQ=255)

                                                                                                            aTGTCGCTGTAGCGGCGGCCGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCCCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACCATTTGGCCACtt                                 >  4:874709/1‑151 (MQ=255)

                                                                                                            aTGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACtt                                 <  1:449080/151‑1 (MQ=255)

                                                                                                            aTGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACtt                                 <  1:62923/151‑1 (MQ=255)

                                                                                                              gTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTc                                <  4:1037186/150‑1 (MQ=255)

                                                                                                              gTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCa                               <  2:1134779/151‑1 (MQ=255)

                                                                                                               tCGCTGTAGCGGCGGCAGTAGTTGATAAAGGAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACtt                                 >  1:71322/1‑148 (MQ=255)

                                                                                                                 gCTGTAGCGGCGGCAGGAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCag                              >  2:1441725/1‑149 (MQ=255)

                                                                                                                    gTAGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGagta                          <  4:429742/150‑1 (MQ=255)

                                                                                                                      aGCGGCGGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTc                       <  4:1209851/151‑1 (MQ=255)

                                                                                                                       gcggcgGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTc                       >  4:711458/1‑150 (MQ=255)

                                                                                                                       gcggcgGCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCa                      <  2:205364/151‑1 (MQ=255)

                                                                                                                        cggcggCAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAg                     <  2:399368/151‑1 (MQ=255)

                                                                                                                         ggcggcAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAgg                    <  2:465114/151‑1 (MQ=255)

                                                                                                                           tggcAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAgggg                  >  2:1214321/2‑151 (MQ=255)

                                                                                                                           cggcAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAgggg                  >  2:1630291/1‑151 (MQ=255)

                                                                                                                           cggcAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAgggg                  >  3:368531/1‑151 (MQ=255)

                                                                                                                            ggcAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGt                 >  4:792876/1‑151 (MQ=255)

                                                                                                                             gcAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTa                <  2:256364/151‑1 (MQ=255)

                                                                                                                              cAGTAGTTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTaa               <  4:725496/151‑1 (MQ=255)

                                                                                                                               agtagtTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTAAt              >  2:558596/1‑151 (MQ=255)

                                                                                                                                  agtTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTAATgg            <  2:710505/150‑1 (MQ=255)

                                                                                                                                  agtTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTAATGGc           >  4:840212/1‑151 (MQ=255)

                                                                                                                                   gtTGATAAAGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTAATGGCg          <  4:248476/151‑1 (MQ=255)

                                                                                                                                         aaaGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTAATGGCGATGGtt    <  2:1284264/151‑1 (MQ=255)

                                                                                                                                          aaGTAGTTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTAATGGCGATGGttt   >  2:997925/1‑151 (MQ=255)

                                                                                                                                           agtagtTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTAATGGCGATGGtttt  >  1:375862/1‑151 (MQ=255)

                                                                                                                                            gtagtTGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTAATGGCGATGGtttt  >  4:110545/1‑150 (MQ=255)

TGCGGATTGTTGCTTTCGCCCAGTCCATCCACCAGGTCAGATGCGCGGATCATGCGCCCGGGAACGTAGCTACCATCGTCGCGAGGCTCCAGCATTACCAGCATCGGCATGTCGCTGTAGCGGCGGCAGTAGTTGATAAAGTAGTCGCTGGGATTATCGAGATGAAACTCTTTTAAAATCACATGGCCCATTGCCATCGCCAGGGCGCTATCAGTGCCTTGTTTCGGTGCCAGCCACTGGTCGCACAATTTGGCCACTTCAGAGTAGTCAGGGGTAATGGCGATGGTTTT  >  NC_000913/1541473‑1541762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`