Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 92,324 A→G E304E (GAA→GAG ftsI → transpeptidase involved in septal peptidoglycan synthesis; penicillin‑binding protein 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091392,3240AG99.1% 420.9 / ‑4.0 116E304E (GAA→GAGftsItranspeptidase involved in septal peptidoglycan synthesis; penicillin‑binding protein 3
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (47/68);  total (48/68)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

Only 100 of 116 total aligned reads displayed.

CAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGAC  >  NC_000913/92177‑92470
                                                                                                                                                   |                                                                                                                                                  
cAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCg                                                                                                                                                 <  3:352787/151‑1 (MQ=255)
  aGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCggg                                                                                                                                               >  3:441040/1‑151 (MQ=255)
  aGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCggg                                                                                                                                               >  2:937051/1‑151 (MQ=255)
    gCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCt                                                                                                                                             <  4:234399/151‑1 (MQ=255)
       gAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCaa                                                                                                                                          <  4:611042/151‑1 (MQ=255)
        aaTCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCaa                                                                                                                                          <  2:149625/150‑1 (MQ=255)
        aaTCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAAc                                                                                                                                         <  3:125300/151‑1 (MQ=255)
           cTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGt                                                                                                                                      >  3:321274/1‑151 (MQ=255)
             ggTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGt                                                                                                                                      <  2:1016148/149‑1 (MQ=255)
                     cGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGAtg                                                                                                                            <  4:9425/151‑1 (MQ=255)
                     cGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGAtg                                                                                                                            <  2:1530629/151‑1 (MQ=255)
                      gTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGAtgg                                                                                                                           >  4:1459469/1‑151 (MQ=255)
                        gCTGGTGGATGTCAACGCCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAGCCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGAtg                                                                                                                            <  2:157521/148‑1 (MQ=255)
                        gCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGAtggtg                                                                                                                         >  2:368622/1‑151 (MQ=255)
                        gCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGAtggt                                                                                                                          <  4:1446935/150‑1 (MQ=255)
                         cTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGAtggtgg                                                                                                                        >  4:1386141/1‑151 (MQ=255)
                          tggtggATGTCAACACCGGTGAAGGGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGAtggtgg                                                                                                                        >  4:429289/1‑150 (MQ=255)
                           ggtggATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGAtggtgg                                                                                                                        <  2:761675/149‑1 (MQ=255)
                             tggATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACGACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAAt                                                                                                                    >  2:200821/1‑151 (MQ=255)
                              ggATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATg                                                                                                                   <  4:939440/151‑1 (MQ=255)
                               gATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGa                                                                                                                  <  2:1220002/151‑1 (MQ=255)
                               gATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGa                                                                                                                  <  1:251345/151‑1 (MQ=255)
                                aTGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGAc                                                                                                                 <  4:1147877/151‑1 (MQ=255)
                                 tGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGAcc                                                                                                                >  1:268713/1‑151 (MQ=255)
                                    cAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACcgcg                                                                                                             <  3:432989/151‑1 (MQ=255)
                                    cAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACcgcg                                                                                                             >  2:1637026/1‑151 (MQ=255)
                                    cAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACcgcg                                                                                                             >  2:1100053/1‑151 (MQ=255)
                                     aaCACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGt                                                                                                            >  4:1042653/1‑151 (MQ=255)
                                      acacCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGtt                                                                                                           >  4:196389/1‑151 (MQ=255)
                                        acCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGc                                                                                                         >  3:194949/1‑151 (MQ=255)
                                            gTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAAcg                                                                                                     >  3:95185/1‑151 (MQ=255)
                                             tGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAAcgt                                                                                                    >  4:609299/1‑151 (MQ=255)
                                                   gCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAAcgtggcgtg                                                                                              <  2:1338436/151‑1 (MQ=255)
                                                   gCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAAcgtggcgt                                                                                               <  2:1626875/150‑1 (MQ=255)
                                                       gCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGc                                                                                          <  4:1005651/151‑1 (MQ=255)
                                                        cGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCg                                                                                         <  2:650362/151‑1 (MQ=255)
                                                         gATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCgg                                                                                        <  4:92501/151‑1 (MQ=255)
                                                          aTGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCggg                                                                                       <  4:740528/151‑1 (MQ=255)
                                                            ggCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGa                                                                                      <  2:494690/150‑1 (MQ=255)
                                                            ggCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGa                                                                                      <  2:901010/150‑1 (MQ=255)
                                                               tAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGaaa                                                                                    <  4:1598208/149‑1 (MQ=255)
                                                                aaCAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGaaaa                                                                                   <  4:306786/149‑1 (MQ=255)
                                                                 aCAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTc                                                                                <  3:197421/151‑1 (MQ=255)
                                                                  cAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCg                                                                               <  2:614215/151‑1 (MQ=255)
                                                                    gCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGt                                                                             >  4:147831/1‑151 (MQ=255)
                                                                     cccGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTa                                                                            <  2:1467368/151‑1 (MQ=255)
                                                                     cccGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTa                                                                            <  4:331692/151‑1 (MQ=255)
                                                                     cccGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGAAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGt                                                                             >  2:668988/1‑150 (MQ=255)
                                                                      ccGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTAc                                                                           <  4:1433675/151‑1 (MQ=255)
                                                                       cGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACt                                                                          <  4:128399/151‑1 (MQ=255)
                                                                        gTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTc                                                                         <  4:498598/151‑1 (MQ=255)
                                                                            tACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAAt                                                                      >  2:143686/1‑150 (MQ=255)
                                                                             aCAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATa                                                                     <  2:1341770/150‑1 (MQ=255)
                                                                              cAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATAc                                                                    <  2:956818/150‑1 (MQ=255)
                                                                                aCCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCAt                                                                 >  3:424850/1‑151 (MQ=255)
                                                                                  ccTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTc                                                               <  1:342532/151‑1 (MQ=255)
                                                                                   cTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGaaa                                                                                    >  3:218204/1‑129 (MQ=255)
                                                                                    tAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCt                                                             >  3:340995/1‑151 (MQ=255)
                                                                                     aaCAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTcca                                                             <  2:1109543/150‑2 (MQ=255)
                                                                                     aaCAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCt                                                             <  1:349628/150‑1 (MQ=255)
                                                                                     aaCAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCAGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCtt                                                            <  2:1129155/151‑1 (MQ=255)
                                                                                        aaTCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATc                                                         <  4:355847/151‑1 (MQ=255)
                                                                                        aaTCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATc                                                         <  4:1293954/151‑1 (MQ=255)
                                                                                         aTCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCg                                                        >  4:691145/1‑151 (MQ=255)
                                                                                         aTCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCg                                                        >  3:10718/1‑151 (MQ=255)
                                                                                           cTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGa                                                       >  3:74485/1‑150 (MQ=255)
                                                                                             gAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGaa                                                      <  4:378231/149‑1 (MQ=255)
                                                                                             gAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGa                                                       <  2:1500377/148‑1 (MQ=255)
                                                                                              aGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAAtt                                                    >  2:1237288/1‑150 (MQ=255)
                                                                                              aGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTa                                                   <  1:442756/151‑1 (MQ=255)
                                                                                               gCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTa                                                   >  4:1291342/1‑150 (MQ=255)
                                                                                                cGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAAc                                                 <  2:1066109/151‑1 (MQ=255)
                                                                                                cGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAAc                                                 <  4:255741/151‑1 (MQ=255)
                                                                                                cGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAAc                                                 >  4:360364/1‑151 (MQ=255)
                                                                                                   cACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGc                                              <  4:1029714/151‑1 (MQ=255)
                                                                                                     cGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGcc                                             >  4:1326286/1‑150 (MQ=255)
                                                                                                         gAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGaa                                        <  2:1414159/151‑1 (MQ=255)
                                                                                                            agagGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATc                                     >  4:1179265/1‑151 (MQ=255)
                                                                                                            agagGCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATc                                     >  2:1048983/1‑151 (MQ=255)
                                                                                                               ggCGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCaaa                                  <  4:173335/151‑1 (MQ=255)
                                                                                                                 cGATGCGTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCaa                                   >  2:1068225/1‑148 (MQ=255)
                                                                                                                       gTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGc                          >  4:1321550/1‑151 (MQ=255)
                                                                                                                       gTAACCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGc                          <  2:706500/151‑1 (MQ=255)
                                                                                                                         aaCCGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCAc                        <  4:1072095/151‑1 (MQ=255)
                                                                                                                            cGTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCt                     <  2:922954/151‑1 (MQ=255)
                                                                                                                             gTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCt                     >  4:422516/1‑150 (MQ=255)
                                                                                                                             gTACCATCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTa                    <  2:1420466/151‑1 (MQ=255)
                                                                                                                                  aTCACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCg               >  3:210903/1‑151 (MQ=255)
                                                                                                                                   tCACCGACGTGTTTGAACCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAg                 >  2:1064067/1‑148 (MQ=255)
                                                                                                                                    cACCGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGa              <  2:621380/150‑1 (MQ=255)
                                                                                                                                      ccGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAAtt           >  1:214920/1‑151 (MQ=255)
                                                                                                                                      ccGACGTGTTTGAGCAGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAAtt           <  4:1177964/151‑1 (MQ=255)
                                                                                                                                       cGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTa          <  4:1345832/151‑1 (MQ=255)
                                                                                                                                       cGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTa          <  4:1540697/151‑1 (MQ=255)
                                                                                                                                       cGACGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTa          <  4:1304917/151‑1 (MQ=255)
                                                                                                                                          cGTGTTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAAcc       >  4:873992/1‑151 (MQ=255)
                                                                                                                                           gtgtTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAAccc      >  4:481628/1‑151 (MQ=255)
                                                                                                                                             gtTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAATCCt     <  1:216122/150‑1 (MQ=255)
                                                                                                                                             gtTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCt     <  4:618258/150‑1 (MQ=255)
                                                                                                                                             gtTTGAGCCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTg    >  4:929689/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGTCAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGATGCGTAACCGTACCATCACCGACGTGTTTGAACCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGTGGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTACAGCGAATTAACCCTGAC  >  NC_000913/92177‑92470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: