Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,797,110 G→A R279W (CGG→TGG)  pheT ← phenylalanine tRNA synthetase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,797,1100GA99.1% 413.8 / NA 108R279W (CGG→TGG) pheTphenylalanine tRNA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (49/58);  minor base C (0/1);  total (49/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

GTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAG  >  NC_000913/1796962‑1797256
                                                                                                                                                    |                                                                                                                                                  
gtTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCcaca                                                                                                                                                  <  2:736479/151‑1 (MQ=255)
gtTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCcaca                                                                                                                                                  <  4:107634/151‑1 (MQ=255)
    cGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCAcaccac                                                                                                                                              <  4:1442539/151‑1 (MQ=255)
    cGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCAcaccac                                                                                                                                              >  4:1585023/1‑151 (MQ=255)
       cATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAAt                                                                                                                                           <  2:1607886/151‑1 (MQ=255)
       cATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAAt                                                                                                                                           >  2:268132/1‑151 (MQ=255)
       cATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAAt                                                                                                                                           <  4:392800/151‑1 (MQ=255)
        aTTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTCCCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATg                                                                                                                                          <  1:440417/151‑1 (MQ=255)
          tCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATgcc                                                                                                                                        >  4:1617064/1‑151 (MQ=255)
          tCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATgc                                                                                                                                         <  3:112601/150‑1 (MQ=255)
          tCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATgc                                                                                                                                         <  1:243382/150‑1 (MQ=255)
           cACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATgccg                                                                                                                                       >  3:313876/1‑151 (MQ=255)
             cGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATgccgcc                                                                                                                                     <  2:175296/151‑1 (MQ=255)
             cGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATgccgc                                                                                                                                      <  1:271261/150‑1 (MQ=255)
                cAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTc                                                                                                                                  <  4:823438/151‑1 (MQ=255)
                 agagTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTc                                                                                                                                  >  4:118972/1‑150 (MQ=255)
                    gtgtTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATg                                                                                                                              <  1:425705/151‑1 (MQ=255)
                     tgtTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGc                                                                                                                             >  4:866221/1‑151 (MQ=255)
                      gtTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCg                                                                                                                            <  1:255143/151‑1 (MQ=255)
                        tCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGa                                                                                                                           <  4:671755/150‑1 (MQ=255)
                         cGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATc                                                                                                                         <  4:468621/151‑1 (MQ=255)
                           ccACCGAGGATGCCGCCCATCGCCAGCGCCTGGTTGTGGTCGGCGATGACCAGAGTTTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCACCACCAGCGTTTCGCCCTCTTTCGCCATCCCCACCACAATGCCGCCTTCAATGCGATCtt                                                                                                                       <  1:420356/151‑1 (MQ=255)
                            cACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCttt                                                                                                                      >  2:728660/1‑151 (MQ=255)
                            cACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCttt                                                                                                                      >  2:609275/1‑151 (MQ=255)
                             aCCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCttt                                                                                                                      >  2:382934/1‑150 (MQ=255)
                                gAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCg                                                                                                                  <  4:1240305/151‑1 (MQ=255)
                                gAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCg                                                                                                                  <  1:143470/151‑1 (MQ=255)
                                  aGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGaa                                                                                                                <  2:555676/151‑1 (MQ=255)
                                    aTGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAg                                                                                                              <  4:20126/151‑1 (MQ=255)
                                     tGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGc                                                                                                             >  2:906868/1‑151 (MQ=255)
                                       ccgccCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGt                                                                                                           <  4:1409109/151‑1 (MQ=255)
                                       ccgccCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGt                                                                                                           <  4:935762/151‑1 (MQ=255)
                                       ccgccCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGt                                                                                                           >  2:676146/1‑151 (MQ=255)
                                        cgccCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTg                                                                                                          <  4:793247/151‑1 (MQ=255)
                                           ccATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCAt                                                                                                       <  4:898149/151‑1 (MQ=255)
                                             aTCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCg                                                                                                     <  3:476516/151‑1 (MQ=255)
                                               cGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATc                                                                                                      <  3:107161/148‑1 (MQ=255)
                                                gCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCt                                                                                                  >  4:502434/1‑151 (MQ=255)
                                                gCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCt                                                                                                  >  4:1112880/1‑151 (MQ=255)
                                                gCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCt                                                                                                  >  2:1465068/1‑151 (MQ=255)
                                                 ccAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTg                                                                                                 >  2:489311/1‑151 (MQ=255)
                                                   aGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGc                                                                                               >  4:1048918/1‑151 (MQ=255)
                                                     cgcCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGcca                                                                                             <  2:782273/151‑2 (MQ=255)
                                                      gcCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCTTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCa                                                                                            <  4:564588/151‑1 (MQ=255)
                                                           gttgtGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTc                                                                                        <  1:434630/150‑1 (MQ=255)
                                                           gttgtGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTc                                                                                        <  2:657828/150‑1 (MQ=255)
                                                           gttgtGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCg                                                                                       >  4:1668452/1‑151 (MQ=255)
                                                            ttgtGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCg                                                                                       >  4:1006670/1‑150 (MQ=255)
                                                                ggTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGagca                                                                                   <  1:177185/150‑1 (MQ=255)
                                                                  tCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGagcagc                                                                                 >  2:1224469/1‑150 (MQ=255)
                                                                   cGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGcac                                                                               >  4:818901/1‑151 (MQ=255)
                                                                    ggCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGcaca                                                                              <  4:377760/151‑1 (MQ=255)
                                                                      cGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACAt                                                                             >  4:1443687/1‑150 (MQ=255)
                                                                       gATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAg                                                                           <  2:69924/151‑1 (MQ=255)
                                                                         tGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGtt                                                                         >  2:950815/1‑151 (MQ=255)
                                                                         tGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGtt                                                                         >  1:14162/1‑151 (MQ=255)
                                                                         tGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGtt                                                                         >  2:382013/1‑151 (MQ=255)
                                                                          gACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTg                                                                        >  4:939034/1‑151 (MQ=255)
                                                                          gACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTg                                                                        >  2:59014/1‑151 (MQ=255)
                                                                          gACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTg                                                                        <  2:1106916/151‑1 (MQ=255)
                                                                            ccAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGt                                                                      <  2:44609/151‑1 (MQ=255)
                                                                             cAGAGTTTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCTCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTg                                                                     <  4:1030286/151‑1 (MQ=255)
                                                                              agagTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGa                                                                    <  4:240963/151‑1 (MQ=255)
                                                                                agTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACg                                                                  <  1:182145/151‑1 (MQ=255)
                                                                                agTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACg                                                                  <  3:295985/151‑1 (MQ=255)
                                                                                  tgtCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTc                                                                >  3:331887/1‑151 (MQ=255)
                                                                                   gtCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTca                                                               <  4:882369/151‑1 (MQ=255)
                                                                                    tCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTca                                                               >  1:331955/1‑150 (MQ=255)
                                                                                     cAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTcaac                                                             <  2:585755/151‑1 (MQ=255)
                                                                                      aGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTcaac                                                             >  4:573361/1‑150 (MQ=255)
                                                                                              gcttcgcttcAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATc                                                    <  4:1648070/151‑1 (MQ=255)
                                                                                               cttcgcttcAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCg                                                   >  1:364770/1‑151 (MQ=255)
                                                                                                ttcgcttcAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATc                                                    >  2:1187397/1‑149 (MQ=255)
                                                                                                  cgcttcAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGAt                                                 >  4:363587/1‑150 (MQ=255)
                                                                                                   gcttcAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGa                                                  >  2:762703/1‑148 (MQ=255)
                                                                                                   gcttcAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAg                                               <  4:1028060/151‑1 (MQ=255)
                                                                                                           aCCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATc                                       <  2:118773/151‑1 (MQ=255)
                                                                                                            ccGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATcc                                      <  2:1246240/151‑1 (MQ=255)
                                                                                                              gTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTCGCCCAGTACGAGCAGCACATAGTTGGTGCCGTCAACAACTGCATCGATAGAACGGATCCCg                                    <  2:143487/151‑1 (MQ=255)
                                                                                                                cGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCg                                    <  2:232601/149‑1 (MQ=255)
                                                                                                                cGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCa                                  <  1:377961/151‑1 (MQ=255)
                                                                                                                  agcagcACCAGCGTTTCACCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAac                                <  4:737196/151‑1 (MQ=255)
                                                                                                                   gcagcaCCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAacg                               >  1:465881/1‑151 (MQ=255)
                                                                                                                     agcaCCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAacgac                             >  1:251835/1‑151 (MQ=255)
                                                                                                                      gcaCCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAacgacg                            >  3:288933/1‑151 (MQ=255)
                                                                                                                        aCCAGCGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGc                           >  1:424973/1‑150 (MQ=255)
                                                                                                                             cGTTTCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGtttt                     <  2:1363905/151‑1 (MQ=255)
                                                                                                                                ttCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCCGTTCGAGCAGCACATAGTTGGTGACGTCAACAACCGCATCGATAGAACGGATCCCGCCACGACGCAGttttttt                  >  1:350698/1‑149 (MQ=255)
                                                                                                                                ttCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTtttct                  >  1:336662/1‑151 (MQ=255)
                                                                                                                                 tCGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTtttctt                 >  4:872641/1‑151 (MQ=255)
                                                                                                                                  cGCCCTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTtttcttt                >  4:336436/1‑151 (MQ=255)
                                                                                                                                     ccTCTTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACACCTGCATCGATAGAACGGATCCCGCAACGACGCATTTTTTCTTTCAt             <  3:104075/151‑1 (MQ=255)
                                                                                                                                      ctctTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATc            >  4:585146/1‑151 (MQ=255)
                                                                                                                                        ctTTCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATc            >  1:235403/1‑149 (MQ=255)
                                                                                                                                          ttCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCcac         <  2:1080516/150‑1 (MQ=255)
                                                                                                                                           tCGCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCcaca        <  4:14371/150‑1 (MQ=255)
                                                                                                                                            tgCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAAAGACGCAGTTTTTCTTTCATCCACAGc      >  2:1334170/2‑151 (MQ=255)
                                                                                                                                             gCCATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCg     <  3:394560/151‑1 (MQ=255)
                                                                                                                                              ccATCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCgg    >  2:506519/1‑151 (MQ=255)
                                                                                                                                                aTCCACACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAg  <  3:229271/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAG  >  NC_000913/1796962‑1797256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: