Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,816,806 A→G R110R (CGT→CGC chbG ← chito‑oligosaccharide deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,816,8060AG99.0% 360.1 / ‑5.2 95R110R (CGT→CGCchbGchito‑oligosaccharide deacetylase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (46/48);  total (47/48)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAA  >  NC_000913/1816658‑1816951
                                                                                                                                                    |                                                                                                                                                 
gCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCg                                                                                                                                                 <  4:654448/151‑1 (MQ=255)
        aaCGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATact                                                                                                                                         <  2:966970/151‑1 (MQ=255)
         aCGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATactg                                                                                                                                        >  2:11911/1‑151 (MQ=255)
          cGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATactg                                                                                                                                        >  1:476334/1‑150 (MQ=255)
           gCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATactgac                                                                                                                                      >  3:369998/1‑151 (MQ=255)
             cATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATactgactg                                                                                                                                    <  3:195984/151‑1 (MQ=255)
               tCTGACGATCCGCCCGCAACGCAATGCCCTACTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGACCAAATAGCTCAATGAAGCGCAAATACtgactgac                                                                                                                                  >  4:1543748/1‑151 (MQ=255)
                 tGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACa                                                                                                                                 >  2:389658/1‑150 (MQ=255)
                  gACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACa                                                                                                                                 <  4:4252/149‑1 (MQ=255)
                      aTCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTcc                                                                                                                           >  4:992181/1‑151 (MQ=255)
                        ccgcccgcAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTg                                                                                                                         <  4:454203/151‑1 (MQ=255)
                         cgcccgcAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTg                                                                                                                         >  4:278089/1‑150 (MQ=255)
                           cccgcAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGt                                                                                                                      <  2:883030/151‑1 (MQ=255)
                            ccgcAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTa                                                                                                                     <  2:574518/151‑1 (MQ=255)
                              gcaacgcaaTGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAAt                                                                                                                   <  1:423108/151‑1 (MQ=255)
                              gcaacgcaaTGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAAt                                                                                                                   >  2:504691/1‑151 (MQ=255)
                                aacgcaaTGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAAttt                                                                                                                 <  2:849975/151‑1 (MQ=255)
                                aacgcaaTGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAAttt                                                                                                                 >  1:295734/1‑151 (MQ=255)
                                  cgcaaTGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATttct                                                                                                               >  2:1486146/1‑151 (MQ=255)
                                  cgcaaTGCCCTGCTCTGCCGCAAACATGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATttct                                                                                                               >  3:456285/1‑151 (MQ=255)
                                   gcaaTGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATttctt                                                                                                              >  4:451859/1‑151 (MQ=255)
                                      aTGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTcc                                                                                                            >  3:326730/1‑150 (MQ=255)
                                      aTGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCa                                                                                                           <  4:1431101/151‑1 (MQ=255)
                                          ccTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCgg                                                                                                       <  2:960775/151‑1 (MQ=255)
                                          ccTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCgg                                                                                                       <  4:768362/151‑1 (MQ=255)
                                             gCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGt                                                                                                      <  1:136700/149‑1 (MQ=255)
                                             gCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGt                                                                                                      <  2:939362/149‑1 (MQ=255)
                                             gCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTaa                                                                                                    <  1:401243/151‑1 (MQ=255)
                                               tctGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGCTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTaa                                                                                                    >  3:50130/1‑149 (MQ=255)
                                                ctGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTaaa                                                                                                   <  1:428570/149‑1 (MQ=255)
                                                 tGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGc                                                                                                 >  2:96170/1‑150 (MQ=255)
                                                  gCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAg                                                                                                  <  2:1331606/148‑1 (MQ=255)
                                                  gCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGc                                                                                                 <  4:1554194/149‑1 (MQ=255)
                                                  gCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCCCCTGTGTAATTTCTTCCAGCGGTaaatc                                                                                                 <  2:1207339/149‑3 (MQ=255)
                                                   ccGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCAtc                                                                                              >  4:40803/1‑151 (MQ=255)
                                                   ccGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCAtc                                                                                              >  2:1593108/1‑151 (MQ=255)
                                                       aaaCCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCAtcttct                                                                                          >  4:1021441/1‑151 (MQ=255)
                                                       aaaCCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCAtcttct                                                                                          <  2:1120981/151‑1 (MQ=255)
                                                            tGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGc                                                                                     >  2:1571120/1‑151 (MQ=255)
                                                              gCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTg                                                                                      <  4:1115163/148‑1 (MQ=255)
                                                                aacaaTCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCaa                                                                                  <  2:995463/150‑1 (MQ=255)
                                                                aacaaTCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAAc                                                                                 >  4:1366271/1‑151 (MQ=255)
                                                                   aaTCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGc                                                                              <  2:899514/151‑1 (MQ=255)
                                                                       gggAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGa                                                                          <  4:160636/151‑1 (MQ=255)
                                                                           aaaTCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATcc                                                                       >  1:455184/1‑150 (MQ=255)
                                                                            aaTCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCAt                                                                     >  4:856764/1‑151 (MQ=255)
                                                                            aaTCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCAt                                                                     <  4:518293/151‑1 (MQ=255)
                                                                                 gCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTcc                                                                >  4:86404/1‑151 (MQ=255)
                                                                                   gggAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTccc                                                               >  4:1455117/1‑150 (MQ=255)
                                                                                   gggAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCa                                                              <  2:1280856/151‑1 (MQ=255)
                                                                                      aaCATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGca                                                           <  2:880541/151‑1 (MQ=255)
                                                                                      aaCATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGc                                                            >  2:932928/1‑150 (MQ=255)
                                                                                       aCATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGcac                                                          <  4:416391/151‑1 (MQ=255)
                                                                                           atGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACAcc                                                       <  4:1030896/150‑1 (MQ=255)
                                                                                             gCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCAt                                                     <  2:1447357/150‑1 (MQ=255)
                                                                                             gCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATc                                                    >  4:628194/1‑151 (MQ=255)
                                                                                              cacaTGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATcg                                                   <  4:811584/151‑1 (MQ=255)
                                                                                              cacaTGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATcg                                                   >  4:1099786/1‑151 (MQ=255)
                                                                                               acaTGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGc                                                                                     >  3:232111/1‑116 (MQ=255)
                                                                                               acaTGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATcgc                                                  <  2:643635/151‑1 (MQ=255)
                                                                                                   gatgatgGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCggg                                               <  4:344621/150‑1 (MQ=255)
                                                                                                   gatgatgGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGt                                              <  4:934137/151‑1 (MQ=255)
                                                                                                   gatgatgGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGt                                              <  2:1235499/151‑1 (MQ=255)
                                                                                                    atgatgGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGtt                                             >  1:193587/1‑151 (MQ=255)
                                                                                                      gatgGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTa                                            <  1:266101/150‑1 (MQ=255)
                                                                                                        tgGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAAcc                                         >  2:736383/1‑151 (MQ=255)
                                                                                                         ggCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAAcc                                         <  4:73735/150‑1 (MQ=255)
                                                                                                          gCTATCAAGATGCGTAGGGTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAAcccc                                       >  2:1361731/1‑151 (MQ=255)
                                                                                                             aTCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCgg                                     <  2:285094/150‑1 (MQ=255)
                                                                                                             aTCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCgg                                     <  3:281106/150‑1 (MQ=255)
                                                                                                             aTCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCgg                                     <  4:1051525/150‑1 (MQ=255)
                                                                                                             aTCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGc                                    <  2:617978/151‑1 (MQ=255)
                                                                                                               cAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCAt                                  <  2:697673/151‑1 (MQ=255)
                                                                                                               cAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCAt                                  >  2:1206020/1‑151 (MQ=255)
                                                                                                                   aTGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCt                              >  2:232756/1‑151 (MQ=255)
                                                                                                                   aTGCGTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCt                              >  3:327901/1‑151 (MQ=255)
                                                                                                                       gTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCa                          <  4:175683/151‑1 (MQ=255)
                                                                                                                       gTAGGTTTGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCa                          <  3:74448/151‑1 (MQ=255)
                                                                                                                             ttGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCt                    >  2:839367/1‑151 (MQ=255)
                                                                                                                             ttGCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCt                    >  1:373446/1‑151 (MQ=255)
                                                                                                                               gCGTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCtt                   >  4:1560377/1‑150 (MQ=255)
                                                                                                                                 gTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTAcc                >  2:1158973/1‑151 (MQ=255)
                                                                                                                                 gTCCAAATAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTAcc                >  2:1382452/1‑151 (MQ=255)
                                                                                                                                 gTCCAAATAGACCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGAAAAACATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTAcc                >  1:357354/1‑151 (MQ=255)
                                                                                                                                      aaTAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCAt             >  2:634523/1‑149 (MQ=255)
                                                                                                                                      aaTAGCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCAt             >  1:31929/1‑149 (MQ=255)
                                                                                                                                          gCTCAGGGAAACGCAGCTGCTGGGTGACAAGGTGATGAGTAATTTCTTCTAGCGGGAGAGCATCTTCTTCTAACAAAAAACAGATCCATTTTCCCAGCACACTATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAgca         >  2:1539396/1‑147 (MQ=255)
                                                                                                                                          gCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTa         >  2:1333391/1‑149 (MQ=255)
                                                                                                                                          gCTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAg       <  3:337275/151‑1 (MQ=255)
                                                                                                                                           cTCAATGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAgg      >  1:25420/1‑151 (MQ=255)
                                                                                                                                              aaTGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACa   <  2:35200/151‑1 (MQ=255)
                                                                                                                                              aaTGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACa   <  4:87578/151‑1 (MQ=255)
                                                                                                                                               aTGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACaa  <  4:1518233/151‑1 (MQ=255)
                                                                                                                                                tGAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACaa  >  2:164126/1‑150 (MQ=255)
                                                                                                                                                 gAAGCGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACaa  >  2:1367681/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTGACTGACAAGCTCCTGAGTAATTTCTTCCAGCGGTAAAGCATCTTCTTCTGCCAACTGCCAGATCCATTTTCCCAGCACACCATCGCGGGTTAACCCCGGCATAGCTGTCAGTGGCTTACCCATAGTAAGGACAA  >  NC_000913/1816658‑1816951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: