Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,867,340 A→T N145Y (AAC→TAC)  yeaG → protein kinase, endogenous substrate unidentified; autokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,867,3400AT98.8% 312.6 / ‑3.4 80N145Y (AAC→TAC) yeaGprotein kinase, endogenous substrate unidentified; autokinase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (39/40);  total (39/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

CAGGGGCTGGAAGAGAAGAAACAAATCCTGTATCTGCTGGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAA  >  NC_000913/1867196‑1867485
                                                                                                                                                |                                                                                                                                                 
cAGGGGCTGGAAGAGAAGAAACAAATCCTGTATCTGCTGGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGatc                                                                                                                                             <  4:1067791/151‑1 (MQ=255)
cAGGGGCTGGAAGAGAAGAAACAAATCCTGTATCTGCTGGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGa                                                                                                                                               <  2:1388163/149‑1 (MQ=255)
 aGGGGCTGGAAGAGAAGAAACAAATCCTGTATCTGCTGGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGatc                                                                                                                                             >  4:75543/1‑150 (MQ=255)
  ggggCTGGAAGAGAAGAAACAAATCCTGTATCTGCTGGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGatcat                                                                                                                                           <  2:645/151‑1 (MQ=255)
     gCTGGAAGAGAAGAAACAAATCCTGTATCTGCTGGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCg                                                                                                                                        >  2:838917/1‑151 (MQ=255)
       tGGAAGAGAAGAAACAAATCCTGTATCTGCTGGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGtt                                                                                                                                      >  1:56958/1‑151 (MQ=255)
         gaagagaagaAACAAATCCTGTATCTGCTGGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTTCCGATTTATGTATTGAGCGCGAACGGTGGGCGTAGCCCGGTCTACGATCATCCGTTCt                                                                                                                                    <  2:290433/151‑1 (MQ=255)
           tgagaagaAACAAATCCTGTATCTGCTGGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGt                                                                                                                                  >  1:78257/2‑151 (MQ=255)
             agaagaAACAAATCCTGTATCTGCTGGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCt                                                                                                                                <  2:746959/151‑1 (MQ=255)
             agaagaAACAAATCCTGTATCTGCTGGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCt                                                                                                                                >  4:101793/1‑151 (MQ=255)
                    aCAAATCCTGTATCTGCTGGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAAt                                                                                                                         <  2:1339941/151‑1 (MQ=255)
                         tCCTGTATCTGCTGGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGc                                                                                                                     >  1:179714/1‑150 (MQ=255)
                          ccTGTATCTGCTGGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAg                                                                                                                   <  3:211579/151‑1 (MQ=255)
                             gTATCTGCTGGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAgg                                                                                                                  <  2:202083/149‑1 (MQ=255)
                                 ctgctgGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATg                                                                                                            >  2:389515/1‑151 (MQ=255)
                                  tgctgGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATgc                                                                                                           >  3:2075/1‑151 (MQ=255)
                                  tgctgGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATgc                                                                                                           >  2:1512395/1‑151 (MQ=255)
                                   gctgGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATgcg                                                                                                          <  4:420186/151‑1 (MQ=255)
                                   gctgGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATgcg                                                                                                          >  4:1596354/1‑151 (MQ=255)
                                   gctgGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATg                                                                                                            <  4:690141/149‑1 (MQ=255)
                                    ctgGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATgcgc                                                                                                         >  3:337942/1‑151 (MQ=255)
                                     tgGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATgcgc                                                                                                         >  2:1422294/1‑150 (MQ=255)
                                      ggggCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCa                                                                                                        <  3:70509/150‑1 (MQ=255)
                                      ggggCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCa                                                                                                        <  2:1360652/150‑1 (MQ=255)
                                        ggCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGAt                                                                                                     <  2:1017632/151‑1 (MQ=255)
                                             gtgggtggGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTgg                                                                                                >  2:1050355/1‑151 (MQ=255)
                                             gtgggtggGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTgg                                                                                                >  4:775946/1‑151 (MQ=255)
                                             gtgggtggGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTg                                                                                                 <  4:629288/150‑1 (MQ=255)
                                             gtgggtggGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTg                                                                                                 <  3:392948/150‑1 (MQ=255)
                                                ggtgggGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGaaa                                                                                             <  3:204314/151‑1 (MQ=255)
                                                 gtgggGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGCTTCTGGaaa                                                                                             >  2:36918/1‑150 (MQ=255)
                                                     gggTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAagag                                                                                        <  2:630644/151‑1 (MQ=255)
                                                      ggTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGt                                                                                       <  2:128673/151‑1 (MQ=255)
                                                         aaaTCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATg                                                                                    <  2:1636868/151‑1 (MQ=255)
                                                         aaaTCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATg                                                                                    >  2:147905/1‑151 (MQ=255)
                                                          aaTCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATgg                                                                                   <  3:375155/151‑1 (MQ=255)
                                                           atcatcGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATggt                                                                                  <  2:20464/151‑2 (MQ=255)
                                                           atcatcGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGc                                                                                  <  1:203105/151‑1 (MQ=255)
                                                            tcatcGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATgg                                                                                   >  2:1334183/1‑149 (MQ=255)
                                                              atcGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCAtt                                                                               >  2:1180715/1‑151 (MQ=255)
                                                              atcGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCAtt                                                                               <  2:1526181/151‑1 (MQ=255)
                                                                 gCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCt                                                                            <  2:598674/151‑1 (MQ=255)
                                                                  cTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTc                                                                           >  2:929140/1‑151 (MQ=255)
                                                                  cTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTc                                                                           >  3:348476/1‑151 (MQ=255)
                                                                        gAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGtt                                                                     >  2:758967/1‑151 (MQ=255)
                                                                        gAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGtt                                                                     >  4:1604476/1‑151 (MQ=255)
                                                                        gAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGtt                                                                     <  2:221879/151‑1 (MQ=255)
                                                                         aGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGtt                                                                     >  2:1139244/1‑150 (MQ=255)
                                                                         aGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGtt                                                                     >  4:1170431/1‑150 (MQ=255)
                                                                           cGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTAtc                                                                  >  2:30989/1‑151 (MQ=255)
                                                                               tGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCgg                                                              >  2:1258690/1‑151 (MQ=255)
                                                                                gAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCgg                                                              <  2:548423/150‑1 (MQ=255)
                                                                                    tCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGTGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCAcc                                                          >  4:1673225/1‑150 (MQ=255)
                                                                                     cATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCGGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACcat                                                        >  2:784883/1‑151 (MQ=255)
                                                                                      aTTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACcatc                                                       >  4:854259/1‑151 (MQ=255)
                                                                                         aaTGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACcatcat                                                     >  2:966232/1‑150 (MQ=255)
                                                                                         aaTGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACcatcat                                                     <  2:65818/150‑1 (MQ=255)
                                                                                         aaTGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATg                                                    >  3:389478/1‑151 (MQ=255)
                                                                                         aaTGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATg                                                    <  3:78261/151‑1 (MQ=255)
                                                                                         aaTGCAGCTCGTACCAACTTATGTATGGAGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTTTTTTTTAAATCCGCAGGAAGCTGCGAAGATTCTGAAAAAAGCCTACGCCTTTCCCCGCCGCTACCTCGTCACgatc                                                       <  2:9881/148‑1 (MQ=255)
                                                                                          aTGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGt                                                   <  2:1267542/151‑1 (MQ=255)
                                                                                            gCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCg                                                 <  4:218672/151‑1 (MQ=255)
                                                                                            gCAGCTCGTACCGATTTAGGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTc                                                  <  2:471693/150‑1 (MQ=255)
                                                                                                cTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGt                                             >  1:204521/1‑151 (MQ=255)
                                                                                                  cGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTgg                                           >  2:458073/1‑151 (MQ=255)
                                                                                                        gATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGggcggc                                      >  3:395066/1‑150 (MQ=255)
                                                                                                         aTTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCa                                     >  3:58601/1‑150 (MQ=255)
                                                                                                           ttATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCaaa                                   <  3:152781/150‑1 (MQ=255)
                                                                                                                 aTTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTg                            >  2:1348216/1‑151 (MQ=255)
                                                                                                                 aTTGAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTg                            >  3:121487/1‑151 (MQ=255)
                                                                                                                    gAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCa                          <  2:576425/150‑1 (MQ=255)
                                                                                                                    gAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCAt                         <  1:160582/151‑1 (MQ=255)
                                                                                                                    gAGCGCGAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCAt                         >  2:145547/1‑151 (MQ=255)
                                                                                                                        gcgAACGGTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAAt                     <  4:1269513/151‑1 (MQ=255)
                                                                                                                              ggTGAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCACTCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTtggtg               >  2:140557/1‑151 (MQ=255)
                                                                                                                                 gAGCGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGc             <  4:1527226/150‑1 (MQ=255)
                                                                                                                                    cGTAGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGata         <  2:679228/151‑1 (MQ=255)
                                                                                                                                       aGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATc       <  4:148160/150‑1 (MQ=255)
                                                                                                                                       aGCCCGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATc       <  1:352584/150‑1 (MQ=255)
                                                                                                                                           cGGTCTACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTaa  <  2:763194/151‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CAGGGGCTGGAAGAGAAGAAACAAATCCTGTATCTGCTGGGGCCTGTGGGTGGGGGTAAATCATCGCTTGCTGAGCGACTGAAATCATTAATGCAGCTCGTACCGATTTATGTATTGAGCGCGAACGGTGAGCGTAGCCCGGTCAACGATCATCCGTTCTGTCTTTTCAATCCGCAGGAAGATGCGCAGATTCTGGAAAAAGAGTATGGCATTCCTCGCCGTTATCTCGGCACCATCATGTCGCCGTGGGCGGCAAAACGCCTGCATGAATTTGGTGGCGATATCACTAA  >  NC_000913/1867196‑1867485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: