Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,920,830	T→C	V203A (GTG→GCG)	rsmF →	16S rRNA m(5)C1407 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,920,830	0	T	C	100.0%	335.9 / NA	92	V203A (GTG→GCG)	rsmF	16S rRNA m(5)C1407 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/48);  total (44/48)

GTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAG  >  NC_000913/1920691‑1920975                                                                                                                                            |                                                                                                                                                  gtgtTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGAt                                                                                                                                         <  4:260687/150‑1 (MQ=255)        aTGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATg                                                                                                                                  <  2:1302722/150‑1 (MQ=255)          gCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATgcgc                                                                                                                               >  4:276185/1‑151 (MQ=255)           ccAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGGTGCGCt                                                                                                                              >  2:1382870/1‑151 (MQ=255)           ccAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATgcgat                                                                                                                              <  4:553654/151‑3 (MQ=255)           ccAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCt                                                                                                                              >  2:644483/1‑151 (MQ=255)            cAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTa                                                                                                                             <  2:1153779/151‑1 (MQ=255)              atatCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTaa                                                                                                                            >  4:764395/1‑150 (MQ=255)              atatCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTaa                                                                                                                            >  2:196620/1‑150 (MQ=255)                atCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTaaaaa                                                                                                                         <  1:473023/151‑1 (MQ=255)                    gCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTg                                                                                                                     >  3:425223/1‑151 (MQ=255)                     ccGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTgg                                                                                                                    >  4:951290/1‑151 (MQ=255)                     ccGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTgg                                                                                                                    >  4:1512433/1‑151 (MQ=255)                           tgGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCAcc                                                                                                               >  3:121109/1‑150 (MQ=255)                            ggTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCa                                                                                                              <  4:198205/150‑1 (MQ=255)                             gTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAg                                                                                                             <  4:1271833/150‑1 (MQ=255)                              tATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGa                                                                                                            >  2:642880/1‑150 (MQ=255)                               aTCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGaaa                                                                                                          <  4:1609166/151‑1 (MQ=255)                               aTCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGaa                                                                                                           <  4:1584998/150‑1 (MQ=255)                                 cAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGc                                                                                                        <  4:776448/151‑1 (MQ=255)                                 cAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGc                                                                                                        >  4:562819/1‑151 (MQ=255)                                    tAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAAt                                                                                                     >  4:1199300/1‑151 (MQ=255)                                      aTGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATc                                                                                                    >  2:375521/1‑150 (MQ=255)                                         ttGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAgg                                                                                                 >  3:138411/1‑150 (MQ=255)                                           gcgcTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGaaa                                                                                              <  2:199765/151‑1 (MQ=255)                                             gcTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATc                                                                                            <  4:1315169/151‑1 (MQ=255)                                             gcTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATc                                                                                            <  4:840070/151‑1 (MQ=255)                                                cacacaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCg                                                                                           <  4:1436660/149‑1 (MQ=255)                                                cacacaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGc                                                                                          >  4:633347/1‑150 (MQ=255)                                                cacacaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGc                                                                                          >  2:865338/1‑150 (MQ=255)                                                 acacaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGc                                                                                          >  4:1438363/1‑149 (MQ=255)                                                 acacaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAg                                                                                        <  4:284683/151‑1 (MQ=255)                                                 acacaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAg                                                                                        <  4:249950/151‑1 (MQ=255)                                                 acacaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAg                                                                                        <  3:364269/151‑1 (MQ=255)                                                    caTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTa                                                                                     <  1:97182/151‑1 (MQ=255)                                                       tttGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTaca                                                                                   <  3:460069/150‑1 (MQ=255)                                                           aTGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTACGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACaa                                                                                <  2:663880/149‑1 (MQ=255)                                                                 gcgTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCt                                                                        <  3:390777/151‑1 (MQ=255)                                                                 gcgTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCt                                                                        <  4:807594/151‑1 (MQ=255)                                                                  cgTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCtt                                                                       >  2:1015386/1‑151 (MQ=255)                                                                       ttGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCg                                                                   >  4:728408/1‑150 (MQ=255)                                                                        tGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGa                                                                  <  4:1328279/150‑1 (MQ=255)                                                                          gTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAg                                                               <  4:1287204/151‑1 (MQ=255)                                                                            gCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAgc                                                              <  4:296091/150‑1 (MQ=255)                                                                             cGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAgcgc                                                            >  4:1160131/1‑151 (MQ=255)                                                                             cGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAgcgc                                                            <  2:690529/151‑1 (MQ=255)                                                                               gCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCt                                                          >  4:620774/1‑151 (MQ=255)                                                                               gCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCt                                                          <  2:189337/151‑1 (MQ=255)                                                                               gCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCt                                                          <  4:196943/151‑1 (MQ=255)                                                                                cAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCtt                                                         >  3:304448/1‑151 (MQ=255)                                                                                 aGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCttt                                                        <  4:577658/151‑1 (MQ=255)                                                                                 aGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCttt                                                        <  4:168407/151‑1 (MQ=255)                                                                                    gCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCAt                                                     <  4:517306/151‑1 (MQ=255)                                                                                       aGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGc                                                   >  2:1623309/1‑150 (MQ=255)                                                                                       aGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCa                                                  <  3:77972/151‑1 (MQ=255)                                                                                         aaaTGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCAtt                                                <  4:911447/151‑1 (MQ=255)                                                                                          aaTGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTa                                               <  3:179298/151‑1 (MQ=255)                                                                                             gTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGt                                            >  4:141993/1‑151 (MQ=255)                                                                                              ttCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTc                                           >  4:1418464/1‑151 (MQ=255)                                                                                               tCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGAAAGCGCCTTTCATGCATTACGAcc                                          >  2:1076366/1‑151 (MQ=255)                                                                                                cGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCt                                         <  2:632497/151‑1 (MQ=255)                                                                                                cGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCt                                         >  2:1668347/1‑151 (MQ=255)                                                                                                cGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCt                                         >  4:1685275/1‑151 (MQ=255)                                                                                                 gATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCtg                                        <  2:1045750/151‑1 (MQ=255)                                                                                                  aTGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCtgg                                       <  2:537937/151‑1 (MQ=255)                                                                                                  aTGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCtgg                                       >  4:1503254/1‑151 (MQ=255)                                                                                                  aTGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCtgg                                       <  3:245730/151‑1 (MQ=255)                                                                                                   tGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCtggt                                      >  4:246884/1‑151 (MQ=255)                                                                                                    gCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCtggtg                                     <  2:1043733/151‑1 (MQ=255)                                                                                                     ccATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCtggtgg                                    >  4:1218711/1‑151 (MQ=255)                                                                                                       aTTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTggtggt                                   >  1:345476/1‑150 (MQ=255)                                                                                                        ttttGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTAc                                 >  4:396518/1‑151 (MQ=255)                                                                                                         tttGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACg                                >  4:360127/1‑151 (MQ=255)                                                                                                         tttGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACg                                >  3:330396/1‑151 (MQ=255)                                                                                                           tGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGc                               <  4:84198/150‑1 (MQ=255)                                                                                                            gCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTg                             <  4:1271485/151‑1 (MQ=255)                                                                                                               ggACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTgg                            <  2:119332/149‑1 (MQ=255)                                                                                                               ggACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGtt                          <  4:1376493/151‑1 (MQ=255)                                                                                                                gACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGttt                         <  1:76285/151‑1 (MQ=255)                                                                                                                gACGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGttt                         <  2:1154449/151‑1 (MQ=255)                                                                                                                 aCGCTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTa                        <  2:947775/151‑1 (MQ=255)                                                                                                                    cTCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACt                      >  4:1356974/1‑150 (MQ=255)                                                                                                                     tCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCg                    >  2:1519130/1‑151 (MQ=255)                                                                                                                     tCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCg                    >  3:405055/1‑151 (MQ=255)                                                                                                                     tCCCTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCg                    >  2:1685474/1‑151 (MQ=255)                                                                                                                      cccTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCg                    >  4:1531798/1‑150 (MQ=255)                                                                                                                      cccTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCg                    <  2:95333/150‑1 (MQ=255)                                                                                                                        cTGCTCTGGCGAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCGAcc                 >  2:1642294/1‑151 (MQ=255)                                                                                                                                  gAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTa        >  2:1221771/1‑150 (MQ=255)                                                                                                                                  gAAGGCGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTa        >  2:1336708/1‑150 (MQ=255)                                                                                                                                       tgtggcgCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAAcc    >  2:507864/2‑149 (MQ=255)                                                                                                                                       cGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAAcc    >  4:1472703/1‑149 (MQ=255)                                                                                                                                       cGTGGCGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAg  <  3:313902/151‑1 (MQ=255)                                                                                                                                            |                                                                                                                                                  GTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAG  >  NC_000913/1920691‑1920975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑