Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,213,613 A→T G344G (GGT→GGA yehU ← inner membrane putative sensory kinase in two‑component system with YehT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,213,6130AT99.0% 387.1 / ‑3.7 99G344G (GGT→GGAyehUinner membrane putative sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (41/57);  total (41/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 101 total aligned reads displayed.

CGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCACCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGGTAAGGT  >  NC_000913/2213466‑2213760
                                                                                                                                                   |                                                                                                                                                   
cGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTccc                                                                                                                                                  <  4:809760/151‑1 (MQ=255)
       aTGGTGTTAAGCGCATTACACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGc                                                                                                                                           >  2:1303936/1‑151 (MQ=255)
         ggTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCgg                                                                                                                                         <  1:30370/151‑1 (MQ=255)
         ggTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCgg                                                                                                                                         <  1:460635/151‑1 (MQ=255)
         ggTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCg                                                                                                                                          <  4:373877/150‑1 (MQ=255)
           tgtTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGtt                                                                                                                                       >  1:33278/1‑151 (MQ=255)
            gtTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGttg                                                                                                                                      <  4:905193/151‑1 (MQ=255)
            gtTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGtt                                                                                                                                       <  2:1316982/150‑1 (MQ=255)
             ttAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGttg                                                                                                                                      >  4:1452732/1‑150 (MQ=255)
               aaGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGttgatt                                                                                                                                   <  4:1412009/151‑1 (MQ=255)
               aaGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGttgatt                                                                                                                                   <  2:135805/151‑1 (MQ=255)
                aGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGttgattg                                                                                                                                  >  2:548869/1‑151 (MQ=255)
                aGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGttgattg                                                                                                                                  <  3:390751/151‑1 (MQ=255)
                 gcgcATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGttgattga                                                                                                                                 <  2:1092568/151‑1 (MQ=255)
                 gcgcATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGttgattg                                                                                                                                  >  3:142771/1‑150 (MQ=255)
                   gcATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAAc                                                                                                                               <  2:1376705/151‑1 (MQ=255)
                   gcATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAAc                                                                                                                               <  2:217092/151‑1 (MQ=255)
                       tAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGa                                                                                                                            >  4:747927/1‑150 (MQ=255)
                       tAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGa                                                                                                                            <  4:1399843/150‑1 (MQ=255)
                        aaaCAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAAt                                                                                                                          <  4:201515/151‑1 (MQ=255)
                           cAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATaaa                                                                                                                       <  1:363232/151‑1 (MQ=255)
                             aaaaaTGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACg                                                                                                                     <  3:167633/151‑1 (MQ=255)
                             aaaaaTGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACg                                                                                                                     >  4:1234337/1‑151 (MQ=255)
                              aaaaTGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACgg                                                                                                                    >  3:243827/1‑151 (MQ=255)
                               aaaTGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGt                                                                                                                   >  4:1580811/1‑151 (MQ=255)
                               aaaTGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGt                                                                                                                   >  4:107617/1‑151 (MQ=255)
                               aaaTGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGt                                                                                                                   <  2:1416753/151‑1 (MQ=255)
                                aaTGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGtt                                                                                                                  <  3:36098/151‑1 (MQ=255)
                                 aTGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGttt                                                                                                                 <  4:1213533/151‑1 (MQ=255)
                                   ggggATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGttttt                                                                                                               >  4:674175/1‑151 (MQ=255)
                                         tCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCtt                                                                                                          <  2:369468/150‑1 (MQ=255)
                                           aCCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCAt                                                                                                       >  4:1534274/1‑151 (MQ=255)
                                             cTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATAc                                                                                                     >  4:1084411/1‑151 (MQ=255)
                                               gggCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACaa                                                                                                   <  2:1285824/151‑1 (MQ=255)
                                               gggCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACa                                                                                                    <  2:548563/150‑1 (MQ=255)
                                                ggCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAAt                                                                                                  <  3:340310/151‑1 (MQ=255)
                                                 gCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAAtt                                                                                                 >  4:1264701/1‑151 (MQ=255)
                                                     gAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGa                                                                                              <  1:142757/150‑1 (MQ=255)
                                                     gAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGAt                                                                                             <  4:1398400/151‑1 (MQ=255)
                                                     gAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGAt                                                                                             >  4:1432808/1‑151 (MQ=255)
                                                     gAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGAt                                                                                             <  4:1256133/151‑1 (MQ=255)
                                                      aaGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATg                                                                                            <  4:665382/151‑1 (MQ=255)
                                                        gCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATgg                                                                                           >  2:971955/1‑150 (MQ=255)
                                                        gCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGt                                                                                          >  4:631172/1‑151 (MQ=255)
                                                        gCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGt                                                                                          <  4:1050480/151‑1 (MQ=255)
                                                         cAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTg                                                                                         <  1:472647/151‑1 (MQ=255)
                                                          aGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGc                                                                                        >  2:470647/1‑151 (MQ=255)
                                                              tGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCa                                                                                     <  2:421666/150‑1 (MQ=255)
                                                                 tctcTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCAc                                                                                 >  3:461005/1‑151 (MQ=255)
                                                                    ctGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCa                                                                                  <  2:49308/147‑1 (MQ=255)
                                                                      gACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCAccc                                                                               <  2:571173/148‑1 (MQ=255)
                                                                       aCTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTg                                                                           >  2:173092/1‑151 (MQ=255)
                                                                         tGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGAt                                                                         >  4:837475/1‑151 (MQ=255)
                                                                            gTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGAtttt                                                                      <  4:66641/151‑1 (MQ=255)
                                                                                 cATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAGAGCGATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATGGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTGcacca                                                                 <  2:1558754/151‑1 (MQ=255)
                                                                                 cATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTcacca                                                                 <  4:904871/151‑1 (MQ=255)
                                                                                  aTCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTcaccac                                                                >  4:1546846/1‑151 (MQ=255)
                                                                                    cGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGc                                                              >  4:1168768/1‑151 (MQ=255)
                                                                                    cGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGc                                                              >  4:926568/1‑151 (MQ=255)
                                                                                     gCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGc                                                              >  2:1099474/1‑150 (MQ=255)
                                                                                     gCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCa                                                             <  2:1274997/151‑1 (MQ=255)
                                                                                         tttGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACgg                                                         >  2:1451743/1‑151 (MQ=255)
                                                                                           tGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGa                                                        >  3:458802/1‑150 (MQ=255)
                                                                                             ccGCTCATATTGCCCGGCAAGGATCTGCGCCTAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAActtaa                                                       <  2:201222/149‑5 (MQ=255)
                                                                                             ccGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAAtt                                                     <  2:1604575/151‑1 (MQ=255)
                                                                                                cTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTAcc                                                  >  3:109746/1‑151 (MQ=255)
                                                                                                cTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTAcc                                                  >  2:188288/1‑151 (MQ=255)
                                                                                                  cATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTAcc                                                  <  2:1152202/149‑1 (MQ=255)
                                                                                                   atatTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGc                                               >  1:181203/1‑151 (MQ=255)
                                                                                                    tatTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCg                                              >  2:530452/1‑151 (MQ=255)
                                                                                                     atTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCg                                              <  2:1381934/150‑1 (MQ=255)
                                                                                                     atTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGt                                             <  4:487151/151‑1 (MQ=255)
                                                                                                       tGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTc                                            <  1:404968/150‑1 (MQ=255)
                                                                                                         cccGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGAc                                         <  2:546563/151‑1 (MQ=255)
                                                                                                          ccGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGAcc                                        >  1:319979/1‑151 (MQ=255)
                                                                                                           cGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGAccc                                       >  3:223497/1‑151 (MQ=255)
                                                                                                               aaGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGt                                   >  2:931431/1‑151 (MQ=255)
                                                                                                                 ggATCTGCGCCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGttt                                 <  2:1674199/151‑1 (MQ=255)
                                                                                                                       gcgcCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTATCAGCGTCGACCCCAGTTTGCATTg                           <  2:189264/151‑1 (MQ=255)
                                                                                                                       gcgcCGAAAGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCAtt                            <  3:176829/150‑1 (MQ=255)
                                                                                                                             aaaGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATg                     >  1:19240/1‑151 (MQ=255)
                                                                                                                              aaGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGc                    >  4:1065481/1‑151 (MQ=255)
                                                                                                                               aGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGc                    >  1:315242/1‑150 (MQ=255)
                                                                                                                               aGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCa                   <  4:1305570/151‑1 (MQ=255)
                                                                                                                               aGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCa                   <  4:169301/151‑1 (MQ=255)
                                                                                                                               aGCAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCa                   <  2:430216/151‑1 (MQ=255)
                                                                                                                                 cAATTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCaa                  >  4:539231/1‑150 (MQ=255)
                                                                                                                                  aaTTGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCaa                  >  2:721581/1‑149 (MQ=255)
                                                                                                                                    ttGCGCAATCCCCTCACCCAACGTGCGGTTAATTGAACTGAATAAACGGTTTTTGGCTTCATACATTTTTATGTTTCCTATCACCCGCTGACTCACACGACGCAACGGTATTACCATCCTCTTCCCCCGTCTGCATTGCTGATGCaacc                <  2:210113/149‑3 (MQ=255)
                                                                                                                                     tGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAagag              <  4:709134/150‑1 (MQ=255)
                                                                                                                                     tGCGCAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGc             >  1:110744/1‑151 (MQ=255)
                                                                                                                                       cgcAATCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCaa           <  1:85295/151‑1 (MQ=255)
                                                                                                                                          aaTCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACgg        <  2:1167294/151‑1 (MQ=255)
                                                                                                                                           aTCCCCTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGGt       <  1:192604/151‑1 (MQ=255)
                                                                                                                                             ccccTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGGTaa     <  4:1489573/151‑1 (MQ=255)
                                                                                                                                             ccccTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGGTa      <  2:630325/150‑1 (MQ=255)
                                                                                                                                              cccTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGGTAAg    <  2:1608807/151‑1 (MQ=255)
                                                                                                                                               ccTCTCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGGTAAgg   <  4:645495/151‑1 (MQ=255)
                                                                                                                                                ctctCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGGTAAGGt  >  1:22171/1‑151 (MQ=255)
                                                                                                                                                ctctCCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGGTAAGGt  >  3:84896/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CGCTTTAATGGTGTTAAGCGCATTAAACAAAAAATGGGGATTCACCTGGGCGTGAAGCAGTTTGATCTCTGACTGGGTGAGCATCGCTTTTTGCCGCTCATATTGCCCGGCAAGGATCTGCGCCGAAAGCAATTGCGCAATCCCCTCACCCAACGTGCGGTTGATTGAACTGAATAAACGGTTTTTGGCTTCATACAATTTGATGGTGCCCATCACCCGCTGATTTTCACCACGCAACGGAATTACCAGCGTCGACCCCAGTTTGCATTGCGGATGCAAAGAGCAACGGTAAGGT  >  NC_000913/2213466‑2213760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: