BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,315,572	C→T	C695C (TGC→TGT)	rcsD →	phosphotransfer intermediate protein in two‑component regulatory system with RcsBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,315,572	0	C	T	100.0%	486.7 / NA	125	C695C (TGC→TGT)	rcsD	phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (54/71); total (54/71)

Only 100 of 127 total aligned reads displayed.

TGAGCGATCAACTGGCACGTAAACTGGGCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTT  >  NC_000913/2315424‑2315716

tGAGCGATCAACTGGCACGTAAACTGGGCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTgtgt                                                                                                                                                >  4:786362/1‑151 (MQ=255)

 gAGCGATCAACTGGCACGTAAACTGGGCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTgtgt                                                                                                                                                >  2:665804/1‑150 (MQ=255)

    cGATCAACTGGCACGTAAACTGGGCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAAtg                                                                                                                                            <  2:807800/151‑1 (MQ=255)

    cGATCAACTGGCACGTAAACTGGGCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAAtg                                                                                                                                            <  4:1065804/151‑1 (MQ=255)

     gATCAACTGGCACGTAAACTGGGCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAAtgg                                                                                                                                           <  4:572947/151‑1 (MQ=255)

     gATCAACTGGCACGTAAACTGGGCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAAtgg                                                                                                                                           >  1:454071/1‑151 (MQ=255)

       tCAACTGGCACGTAAACTGGGCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGCTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGCAAtggtg                                                                                                                                         >  2:1642652/1‑151 (MQ=255)

        cAACTGGCACGTAAACTGGGCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAAtggtgg                                                                                                                                        <  3:454091/151‑1 (MQ=255)

          aCTGGCACGTAAACTGGGCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGAt                                                                                                                                      <  1:93765/151‑1 (MQ=255)

          aCTGGCACGTAAACTGGGCGGTAATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGa                                                                                                                                       <  3:473033/150‑1 (MQ=255)

                aCGTAAACTGGGCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACt                                                                                                                                <  2:1616135/151‑1 (MQ=255)

                 cGTAAACTGGGCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACtt                                                                                                                               >  2:766704/1‑151 (MQ=255)

                    aaaCTGGGCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCgg                                                                                                                            >  2:26695/1‑151 (MQ=255)

                    aaaCTGGGCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCg                                                                                                                             <  2:1265475/150‑1 (MQ=255)

                     aaCTGGGCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGc                                                                                                                           >  4:366810/1‑151 (MQ=255)

                      aCTGGGCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCa                                                                                                                          <  4:1454545/151‑1 (MQ=255)

                      aCTGGGCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCa                                                                                                                          <  4:42708/151‑1 (MQ=255)

                         gggCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGaa                                                                                                                       <  4:1335453/151‑1 (MQ=255)

                         gggCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGa                                                                                                                        >  2:548815/1‑150 (MQ=255)

                            cGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAAtt                                                                                                                    <  1:170241/151‑1 (MQ=255)

                             ggTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTc                                                                                                                   <  2:1586615/151‑1 (MQ=255)

                              gTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCg                                                                                                                  >  4:383381/1‑151 (MQ=255)

                              gTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCg                                                                                                                  >  2:542840/1‑151 (MQ=255)

                                cATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCgg                                                                                                                 >  3:175290/1‑150 (MQ=255)

                                  tttAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAat                                                                                                              >  3:353770/1‑151 (MQ=255)

                                   ttAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAat                                                                                                              >  2:1204972/1‑150 (MQ=255)

                                     aaaCATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAatat                                                                                                            <  2:1338879/150‑1 (MQ=255)

                                       aCATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGt                                                                                                         <  1:194719/151‑1 (MQ=255)

                                       aCATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGt                                                                                                         >  4:580141/1‑151 (MQ=255)

                                           cAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACt                                                                                                     >  4:1312834/1‑151 (MQ=255)

                                                cgcgGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCa                                                                                                <  1:247108/151‑1 (MQ=255)

                                                  cgGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGt                                                                                              >  2:559470/1‑151 (MQ=255)

                                                  cgGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGt                                                                                              <  2:1458014/151‑1 (MQ=255)

                                                   gggATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGtt                                                                                             >  4:882691/1‑151 (MQ=255)

                                                   gggATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGtt                                                                                             <  2:366264/151‑1 (MQ=255)

                                                     gATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGTAATATTGTCACTCGCCAGTTAg                                                                                           <  4:304484/151‑1 (MQ=255)

                                                      aTGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGtt                                                                                             >  4:403324/1‑148 (MQ=255)

                                                             tGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAAtt                                                                                     <  1:300342/149‑1 (MQ=255)

                                                             tGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTgg                                                                                   >  2:1129373/1‑151 (MQ=255)

                                                              ggTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTgg                                                                                   <  3:383535/150‑1 (MQ=255)

                                                               gTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTgggg                                                                                 <  2:385533/151‑1 (MQ=255)

                                                                    cGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCa                                                                             >  2:950545/1‑150 (MQ=255)

                                                                    cGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCa                                                                             >  4:1326803/1‑150 (MQ=255)

                                                                           ctGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTAt                                                                     >  2:50417/1‑151 (MQ=255)

                                                                            tgtgCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATc                                                                    >  4:1630916/1‑151 (MQ=255)

                                                                             gtgCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTa                                                                      <  2:1263206/148‑1 (MQ=255)

                                                                             gtgCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATc                                                                    >  4:1477585/1‑150 (MQ=255)

                                                                              tgCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATcac                                                                  >  2:48785/1‑151 (MQ=255)

                                                                                cATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCacac                                                                <  4:1049827/151‑1 (MQ=255)

                                                                                 atatCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACAcc                                                               <  3:179508/151‑1 (MQ=255)

                                                                                   atCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACAccc                                                              <  1:423406/150‑1 (MQ=255)

                                                                                   atCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACAccc                                                              <  2:540400/150‑1 (MQ=255)

                                                                                   atCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCg                                                             <  2:216428/151‑1 (MQ=255)

                                                                                      aaaaTGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACAcc                                                               <  2:1054071/146‑1 (MQ=255)

                                                                                      aaaaTGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACAcc                                                               >  4:1055422/1‑146 (MQ=255)

                                                                                       aaaTGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATg                                                          >  4:546311/1‑150 (MQ=255)

                                                                                         aTGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGaa                                                        >  4:177511/1‑150 (MQ=255)

                                                                                         aTGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGaa                                                        >  2:392106/1‑150 (MQ=255)

                                                                                          tGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGaa                                                        >  4:1416703/1‑149 (MQ=255)

                                                                                           gCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGaa                                                        >  3:158306/1‑148 (MQ=255)

                                                                                            cTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGaa                                                        >  2:813321/1‑147 (MQ=255)

                                                                                             tCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGatt                                                   >  4:728487/1‑151 (MQ=255)

                                                                                              cGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGatt                                                   <  1:187249/150‑1 (MQ=255)

                                                                                               gCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGatta                                                  >  2:535912/1‑150 (MQ=255)

                                                                                               gCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGatta                                                  >  3:304676/1‑150 (MQ=255)

                                                                                                cAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGattaat                                                >  2:200732/1‑151 (MQ=255)

                                                                                                cAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGattaat                                                >  2:941434/1‑151 (MQ=255)

                                                                                                    tGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGt                                            >  4:1106650/1‑151 (MQ=255)

                                                                                                     gACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTc                                           <  4:684251/151‑1 (MQ=255)

                                                                                                     gACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTc                                           >  1:241310/1‑151 (MQ=255)

                                                                                                      aCCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCa                                          <  4:1034724/151‑1 (MQ=255)

                                                                                                       cccGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCa                                          >  2:3439/1‑150 (MQ=255)

                                                                                                        ccGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAg                                        <  2:36694/151‑1 (MQ=255)

                                                                                                         cGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAAATAGTCAAGa                                       <  2:639789/151‑1 (MQ=255)

                                                                                                           gAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGAtt                                     >  4:1659563/1‑151 (MQ=255)

                                                                                                             aGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTAt                                   <  4:255132/151‑1 (MQ=255)

                                                                                                              gTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATg                                  <  4:774159/151‑1 (MQ=255)

                                                                                                                  aaGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGatat                              <  2:785330/151‑1 (MQ=255)

                                                                                                                  aaGAGGAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGatat                              <  2:357491/151‑1 (MQ=255)

                                                                                                                      ggAAGAAGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCttt                          >  4:923576/1‑151 (MQ=255)

                                                                                                                       gaagaaGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCtttt                         <  4:868457/151‑1 (MQ=255)

                                                                                                                       gaagaaGAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCtttt                         <  4:709448/151‑1 (MQ=255)

                                                                                                                        aagaagAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGTTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCtttt                         <  2:440360/150‑1 (MQ=255)

                                                                                                                        aagaagAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCttttt                        <  3:84641/151‑1 (MQ=255)

                                                                                                                        aagaagAGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCttttt                        >  1:394130/1‑151 (MQ=255)

                                                                                                                           aagaGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAAc                     <  1:257533/151‑1 (MQ=255)

                                                                                                                           aagaGCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAAc                     >  2:1663929/1‑151 (MQ=255)

                                                                                                                              agCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACgg                   >  2:394030/1‑150 (MQ=255)

                                                                                                                               gCGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGAt                 >  4:1620662/1‑151 (MQ=255)

                                                                                                                                cGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGAt                 >  2:653212/1‑150 (MQ=255)

                                                                                                                                cGTTTACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATa                <  2:967835/151‑1 (MQ=255)

                                                                                                                                  tttACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAAt              >  2:699726/1‑151 (MQ=255)

                                                                                                                                    tACTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATcc            >  4:1482800/1‑151 (MQ=255)

                                                                                                                                     aCTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATcc            <  4:543591/150‑1 (MQ=255)

                                                                                                                                     aCTGGATGATGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATcc            <  4:513843/150‑1 (MQ=255)

                                                                                                                                         gatgatGTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTa       <  2:934119/151‑1 (MQ=255)

                                                                                                                                           tgatgTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAAt     >  4:254869/1‑151 (MQ=255)

                                                                                                                                            gatgTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAAt     <  4:1201435/150‑1 (MQ=255)

                                                                                                                                             atgTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCt   <  1:175276/151‑1 (MQ=255)

                                                                                                                                               gTCTGTGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCtt  <  4:666716/150‑1 (MQ=255)

TGAGCGATCAACTGGCACGTAAACTGGGCGGTCATTTAAACATCAAAACGCGGGATGGGCTTGGTACACGCTACTCTGTGCATATCAAAATGCTCGCAGCTGACCCGGAAGTTGAAGAGGAAGAAGAGCGTTTACTGGATGATGTCTGCGTAATGGTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTT  >  NC_000913/2315424‑2315716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`