Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,315,727 G→A G747D (GGC→GAC)  rcsD → phosphotransfer intermediate protein in two‑component regulatory system with RcsBC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,315,7270GA99.1% 430.1 / NA 113G747D (GGC→GAC) rcsDphosphotransfer intermediate protein in two‑component regulatory system with RcsBC
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (65/47);  total (65/48)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

GTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAAGAGGTGACAGAATCGCC  >  NC_000913/2315579‑2315874
                                                                                                                                                    |                                                                                                                                                   
gtggATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGact                                                                                                                                                   >  3:461255/1‑151 (MQ=255)
  ggATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGacttg                                                                                                                                                 >  2:643548/1‑151 (MQ=255)
   gATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGacttgc                                                                                                                                                >  2:174354/1‑151 (MQ=255)
    aTGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGacttgct                                                                                                                                               <  2:898349/151‑1 (MQ=255)
    aTGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGacttgct                                                                                                                                               >  2:259641/1‑151 (MQ=255)
     tGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGacttgctt                                                                                                                                              >  1:317851/1‑151 (MQ=255)
        tACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCtttt                                                                                                                                            <  4:752414/150‑1 (MQ=255)
           ttCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGc                                                                                                                                        >  4:789540/1‑151 (MQ=255)
             cGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCg                                                                                                                                       >  1:184153/1‑150 (MQ=255)
             cGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCg                                                                                                                                       <  4:283143/150‑1 (MQ=255)
                cAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCgatg                                                                                                                                    >  4:989583/1‑150 (MQ=255)
                  gAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGatgatg                                                                                                                                 >  1:123569/1‑151 (MQ=255)
                   aaaTTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGatgatg                                                                                                                                 >  4:815819/1‑150 (MQ=255)
                   aaaTTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGatgatg                                                                                                                                 >  2:1276030/1‑150 (MQ=255)
                   aaaTTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGAtgatga                                                                                                                                <  4:392667/151‑1 (MQ=255)
                   aaaTTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGAtgatga                                                                                                                                <  4:700672/151‑1 (MQ=255)
                        cGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCt                                                                                                                            <  2:1289629/150‑1 (MQ=255)
                        cGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTg                                                                                                                           <  4:394595/151‑1 (MQ=255)
                         tgAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTgg                                                                                                                          >  2:966043/2‑151 (MQ=255)
                           aaTATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCg                                                                                                                        <  4:814401/151‑1 (MQ=255)
                            atatTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGt                                                                                                                       >  4:521501/1‑151 (MQ=255)
                             tatTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGt                                                                                                                       >  2:1542243/1‑150 (MQ=255)
                              atTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTAc                                                                                                                     >  1:313101/1‑151 (MQ=255)
                              atTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAACGATGATGAGTCTGGCGTAc                                                                                                                     >  2:1068951/1‑151 (MQ=255)
                                tGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACg                                                                                                                    <  1:271019/150‑1 (MQ=255)
                                 gTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACggg                                                                                                                  <  4:50516/151‑1 (MQ=255)
                                       cGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAAtt                                                                                                             <  4:1130811/150‑1 (MQ=255)
                                       cGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTg                                                                                                            <  1:467604/151‑1 (MQ=255)
                                         ccAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTggg                                                                                                          >  2:548919/1‑151 (MQ=255)
                                          cAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGc                                                                                                         >  2:1577005/1‑151 (MQ=255)
                                           aGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGcc                                                                                                        >  2:758029/1‑151 (MQ=255)
                                           aGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGACGATGAGTCTGGCGTACGGGAAATTGGGcc                                                                                                        >  4:246287/1‑151 (MQ=255)
                                               aGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTgg                                                                                                     <  2:177217/150‑1 (MQ=255)
                                                 aaaaTTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTc                                                                                                   <  2:727509/150‑1 (MQ=255)
                                                     ttGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATtg                                                                                              >  3:268081/1‑151 (MQ=255)
                                                          gTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGt                                                                                         <  4:201065/151‑1 (MQ=255)
                                                           tGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTc                                                                                        >  4:610085/1‑151 (MQ=255)
                                                           tGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTc                                                                                        >  4:1556462/1‑151 (MQ=255)
                                                            gCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCa                                                                                       <  2:1475739/151‑1 (MQ=255)
                                                             cAACCTGTATCACACCCGATGAAAGATTAATGAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTACTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGATCAATTGTGCGTCaa                                                                                      <  3:133634/151‑1 (MQ=255)
                                                              aaCCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAAc                                                                                     >  1:152333/1‑151 (MQ=255)
                                                                   gTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCa                                                                                 >  3:405800/1‑150 (MQ=255)
                                                                     aTCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAat                                                                               >  4:635697/1‑150 (MQ=255)
                                                                     aTCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAat                                                                               >  3:326060/1‑150 (MQ=255)
                                                                        acacCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATg                                                                            >  2:1359306/1‑150 (MQ=255)
                                                                         cacCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAg                                                                          <  3:276816/151‑1 (MQ=255)
                                                                          acCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGc                                                                         <  3:313118/151‑1 (MQ=255)
                                                                           cccGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCa                                                                        <  2:1325148/151‑1 (MQ=255)
                                                                            ccGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCaa                                                                       <  4:536652/151‑1 (MQ=255)
                                                                              gATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACg                                                                     >  4:401472/1‑151 (MQ=255)
                                                                              gATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACg                                                                     >  4:607431/1‑151 (MQ=255)
                                                                                 gAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTa                                                                  <  1:461235/151‑1 (MQ=255)
                                                                                  aaaGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTAt                                                                 >  4:1306700/1‑151 (MQ=255)
                                                                                   aaGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATg                                                                >  2:1605994/1‑151 (MQ=255)
                                                                                    aGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGc                                                               >  4:1660474/1‑151 (MQ=255)
                                                                                      attaattaGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGc                                                               >  2:492649/1‑149 (MQ=255)
                                                                                      attaattaGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAg                                                             <  2:1311192/151‑1 (MQ=255)
                                                                                      attaattaGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAg                                                             <  2:402740/151‑1 (MQ=255)
                                                                                           ttaGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGc                                                        >  2:267105/1‑151 (MQ=255)
                                                                                            taGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCg                                                       >  1:436123/1‑151 (MQ=255)
                                                                                             aGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCgg                                                      <  4:827259/151‑1 (MQ=255)
                                                                                             aGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCgg                                                      >  4:856349/1‑151 (MQ=255)
                                                                                               tCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTc                                                    >  3:46731/1‑151 (MQ=255)
                                                                                                cAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTc                                                    <  3:448625/150‑1 (MQ=255)
                                                                                                cAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCt                                                   <  2:1657549/151‑1 (MQ=255)
                                                                                                cAAGATTATGATATATTTTTAACGGCTAATGCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGACATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTc                                                    >  1:200665/1‑150 (MQ=255)
                                                                                                 aaGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCtt                                                  >  2:184748/1‑151 (MQ=255)
                                                                                                   gATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTAc                                                >  4:1630890/1‑151 (MQ=255)
                                                                                                   gATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTAc                                                >  2:948246/1‑151 (MQ=255)
                                                                                                        tGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACaatt                                            >  2:27353/1‑150 (MQ=255)
                                                                                                         gATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACaatta                                           >  2:1277560/1‑150 (MQ=255)
                                                                                                            atCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACaattaatt                                        <  3:218305/150‑1 (MQ=255)
                                                                                                                ttttTACGGATAATCCCTCTAATCTTAATGGCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGCGGGTCAACTTTAATATTAGCAACGCTATGCAGGAAGCTGTCTTACAATTAATTGAAg                                    <  4:1644655/150‑1 (MQ=255)
                                                                                                                ttttAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAg                                    >  4:487216/1‑150 (MQ=255)
                                                                                                                ttttAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGt                                   >  2:1481184/1‑151 (MQ=255)
                                                                                                                  ttAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTg                                  <  2:1414933/150‑1 (MQ=255)
                                                                                                                  ttAACGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGc                                 >  2:315239/1‑151 (MQ=255)
                                                                                                                    aaCGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCa                                <  2:922394/150‑1 (MQ=255)
                                                                                                                     aCGGATAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAAc                              >  2:1380667/1‑151 (MQ=255)
                                                                                                                         aTAATCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGgc                          <  4:942654/151‑1 (MQ=255)
                                                                                                                           aaTCCGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGgcgc                        <  4:48880/151‑1 (MQ=255)
                                                                                                                              ccGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAg                      <  4:644418/150‑1 (MQ=255)
                                                                                                                               cGTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGACAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGa                    <  2:1186978/151‑1 (MQ=255)
                                                                                                                                gTCTAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGaa                   <  4:174641/151‑1 (MQ=255)
                                                                                                                                   tAATCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAag                  >  2:408136/1‑149 (MQ=255)
                                                                                                                                    aaTCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAagag                <  3:439742/150‑1 (MQ=255)
                                                                                                                                    aaTCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAAGAgg               >  1:255050/1‑151 (MQ=255)
                                                                                                                                     aTCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAAGAGGt              >  4:108880/1‑151 (MQ=255)
                                                                                                                                      tCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAAGAGGTg             >  1:161641/1‑151 (MQ=255)
                                                                                                                                      tCTTACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAAGAGGTg             >  2:798876/1‑151 (MQ=255)
                                                                                                                                         tACTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAAGAGGTGAc           >  1:304244/1‑150 (MQ=255)
                                                                                                                                          aCTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAAGAGGTGACa          <  2:424127/150‑1 (MQ=255)
                                                                                                                                          aCTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAAGAGGTGACa          <  4:1035187/150‑1 (MQ=255)
                                                                                                                                           cTGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAAGAGGTGAc           >  1:437828/1‑148 (MQ=255)
                                                                                                                                            tGCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGAGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAAGAGGTGACAGa        >  4:935734/1‑150 (MQ=255)
                                                                                                                                             gCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAAGAGGTGACAGaa       <  4:1310970/150‑1 (MQ=255)
                                                                                                                                             gCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAAGAGGTGACAGAAt      <  2:254465/151‑1 (MQ=255)
                                                                                                                                             gCCTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAAGAGGTGACAGAAt      >  4:1032708/1‑151 (MQ=255)
                                                                                                                                              ccTCTGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAAGAGGTGACAGAATc     >  4:385883/1‑151 (MQ=255)
                                                                                                                                                 ctGACTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAAGAGGTGACAGAATCGcc  >  1:413460/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTGGATGTTACTTCGGCAGAAATTCGGAATATTGTCACTCGCCAGTTAGAAAATTGGGGTGCAACCTGTATCACACCCGATGAAAGATTAATTAGTCAAGATTATGATATCTTTTTAACGGATAATCCGTCTAATCTTACTGCCTCTGGCTTGCTTTTAAGCGATGATGAGTCTGGCGTACGGGAAATTGGGCCTGGTCAATTGTGCGTCAACTTCAATATGAGCAACGCTATGCAGGAAGCGGTCTTACAATTAATTGAAGTGCAACTGGCGCAGGAAGAGGTGACAGAATCGCC  >  NC_000913/2315579‑2315874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: