Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,371,563 T→C V219A (GTT→GCT)  arnT → 4‑amino‑4‑deoxy‑L‑arabinose transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,371,5630TC98.9% 319.4 / NA 92V219A (GTT→GCT) arnT4‑amino‑4‑deoxy‑L‑arabinose transferase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (46/45);  total (46/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

ATCACCTGCGGCATGGGGGTGATGACCAAAGGTTTTCTCGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGTTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTGGTACTACGTGCCGGTCATCATTGCCGGTA  >  NC_000913/2371415‑2371709
                                                                                                                                                    |                                                                                                                                                  
aTCACCTGCGGCATGGGGGTGATGACCAAAGGTTTTCTCGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCtc                                                                                                                                                  >  4:1467923/1‑151 (MQ=255)
  cACCTGCGGCATGGGGGTGATGACCAAAGGTTTTCTCGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGctct                                                                                                                                                 <  2:1094512/150‑1 (MQ=255)
  cACCTGCGGCATGGGGGTGATGACCAAAGGTTTTCTCGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGctct                                                                                                                                                 >  4:1619631/1‑150 (MQ=255)
  cACCTGCGGCATGGGGGTGATGACCAAAGGTTTTCTCGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGctct                                                                                                                                                 <  2:737380/150‑1 (MQ=255)
   aCCTGCGGCATGGGGGTGATGACCAAAGGTTTTCTCGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGCTATCAGTTGCGTACTGACGGCTCTcc                                                                                                                                               >  2:416811/1‑151 (MQ=255)
    ccTGCGGCATGGGGGTGATGACCAAAGGTTTTCTCGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTccc                                                                                                                                              <  2:1651107/151‑1 (MQ=255)
              gggggTGATGACCAAAGGTTTTCTCGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACt                                                                                                                                     <  4:145416/150‑1 (MQ=255)
               ggggTGATGACCAAAGGTTTTCTCGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACt                                                                                                                                     <  2:853639/149‑1 (MQ=255)
                gggTGATGACCAAAGGTTTTCTCGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTgg                                                                                                                                   >  4:1000250/1‑150 (MQ=255)
                 ggTGATGACCAAAGGTTTTCTCGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCg                                                                                                                                 <  4:1438249/151‑1 (MQ=255)
                    gatgaCCAAAGGTTTTCTCGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGAt                                                                                                                               >  4:1567855/1‑150 (MQ=255)
                     atgaCCAAAGGTTTTCTCGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGa                                                                                                                                >  2:671602/1‑148 (MQ=255)
                     atgaCCAAAGGTTTTCTCGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGAt                                                                                                                               >  4:660754/1‑149 (MQ=255)
                      tgaCCAAAGGTTTTCTCGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAg                                                                                                                             >  4:1357250/1‑150 (MQ=255)
                      tgaCCAAAGGTTTTCTCGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAg                                                                                                                             >  4:574934/1‑150 (MQ=255)
                              ggTTTTCTCGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCggg                                                                                                                    <  4:1423523/151‑1 (MQ=255)
                                     tcGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGGGCCTa                                                                                                              >  2:1000768/1‑150 (MQ=255)
                                         ccTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTc                                                                                                         <  4:48804/151‑1 (MQ=255)
                                           ttGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCt                                                                                                        >  2:566133/1‑150 (MQ=255)
                                            tgccgtgccgGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCt                                                                                                        <  4:392120/149‑1 (MQ=255)
                                            tgccgtgccgGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTg                                                                                                       <  4:372137/150‑1 (MQ=255)
                                            tgccgtgccgGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTg                                                                                                       <  4:54841/150‑1 (MQ=255)
                                            tgccgtgccgGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACAGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCt                                                                                                        <  4:181543/149‑1 (MQ=255)
                                             gccgtgccgGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTgg                                                                                                      <  4:1321975/150‑1 (MQ=255)
                                              ccgtgccgGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGc                                                                                                     >  2:956840/1‑150 (MQ=255)
                                              ccgtgccgGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGc                                                                                                     >  3:8177/1‑150 (MQ=255)
                                                 tgccgGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACt                                                                                                  >  4:1112944/1‑150 (MQ=255)
                                                 tgccgGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACt                                                                                                  >  4:312012/1‑150 (MQ=255)
                                                  gccgGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTAt                                                                                                <  2:683242/151‑1 (MQ=255)
                                                      gTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTAttttt                                                                                            <  2:1637945/151‑1 (MQ=255)
                                                      gTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTAttttt                                                                                            <  4:703737/151‑1 (MQ=255)
                                                       tATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTAtttttt                                                                                           >  2:408970/1‑151 (MQ=255)
                                                        aTTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTAtttttt                                                                                           <  1:252149/150‑1 (MQ=255)
                                                        aTTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTc                                                                                          <  4:1037075/151‑1 (MQ=255)
                                                           aaGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTgg                                                                                       <  4:990114/151‑1 (MQ=255)
                                                           aaGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAAATTCTGGCACTATTTTTTCTgg                                                                                       >  4:1655038/1‑151 (MQ=255)
                                                             gCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTggg                                                                                      >  1:347584/1‑150 (MQ=255)
                                                             gCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGt                                                                                     <  4:303249/151‑1 (MQ=255)
                                                                tgctgcCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTg                                                                                   >  1:103896/1‑150 (MQ=255)
                                                                 gctgcCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAg                                                                                 <  4:1360893/151‑1 (MQ=255)
                                                                    gcCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCat                                                                              <  4:1533640/151‑1 (MQ=255)
                                                                    gcCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCat                                                                              <  4:934113/151‑1 (MQ=255)
                                                                     ccATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCata                                                                             <  1:208160/151‑1 (MQ=255)
                                                                      cATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCatat                                                                            >  4:707615/1‑151 (MQ=255)
                                                                      cATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCatat                                                                            >  1:42690/1‑151 (MQ=255)
                                                                          ggTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCa                                                                         >  2:375941/1‑150 (MQ=255)
                                                                           gTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAAc                                                                       <  4:1639319/151‑1 (MQ=255)
                                                                             aGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGc                                                                     >  2:1208197/1‑151 (MQ=255)
                                                                              gCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCt                                                                    <  4:1613860/151‑1 (MQ=255)
                                                                               cGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCtt                                                                   >  4:386578/1‑151 (MQ=255)
                                                                                gACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCttt                                                                  <  3:252270/151‑1 (MQ=255)
                                                                                 aCGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCttt                                                                  >  2:1321678/1‑150 (MQ=255)
                                                                                 aCGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTg                                                                 <  3:347601/151‑1 (MQ=255)
                                                                                 aCGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTg                                                                 >  4:405153/1‑151 (MQ=255)
                                                                                   gCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGc                                                                <  2:388587/150‑1 (MQ=255)
                                                                                   gCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGATGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGc                                                                >  3:64024/1‑150 (MQ=255)
                                                                                     aaaaaCGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGCTGCGTACCGCCGGCTCTCCCTTGGGGACTGGCGAAAGCGCAACGGGAGCCTAACTTCTGGGACTATTTTTTCTGGGGTGAGCATATTCAACGCTTTGCACt                                                             >  1:375997/1‑151 (MQ=255)
                                                                                          cGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCCGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATTTTCAACGCTTTGCACTGgat                                                         >  4:1396091/1‑150 (MQ=255)
                                                                                          cGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGgatg                                                        <  2:1589170/151‑1 (MQ=255)
                                                                                          cGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGgatg                                                        <  2:1617658/151‑1 (MQ=255)
                                                                                           gCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGGACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGgatg                                                        >  1:50191/1‑150 (MQ=255)
                                                                                               gAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGTGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGcc                                                   >  2:1648758/1‑151 (MQ=255)
                                                                                                 aaGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCa                                                 <  2:535455/151‑1 (MQ=255)
                                                                                                  aGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCa                                                 >  1:22115/1‑150 (MQ=255)
                                                                                                    acctctTTATTTTCGGCTGGCTGTCGGTTATCAGTTGCGTACTGACGTTTCTCACTTGTGGTCTGGCTATAGCTCATTTGGATCCTAACGTCTCGCACGATTTTTTCTCTTTTGACCATCTTCTTCGCCCTGCACTGGCTGTTTCCCAAcc                                              <  2:1435586/149‑2 (MQ=255)
                                                                                                       tctTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACAt                                             >  4:684190/1‑149 (MQ=255)
                                                                                                        ctTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAAc                                               <  2:1653030/146‑1 (MQ=255)
                                                                                                        ctTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACa                                              >  1:416623/1‑147 (MQ=255)
                                                                                                        ctTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACAt                                             <  4:637458/148‑1 (MQ=255)
                                                                                                          ttatttaCGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATaga                                          <  2:915512/149‑1 (MQ=255)
                                                                                                          ttatttaCGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATaga                                          <  3:452856/149‑1 (MQ=255)
                                                                                                          ttatttaCGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATag                                           <  2:998953/148‑1 (MQ=255)
                                                                                                            atttaCGGCTGGCTGGCGATTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTc                                      <  4:815606/151‑1 (MQ=255)
                                                                                                               taCGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCg                                    >  2:645106/1‑150 (MQ=255)
                                                                                                                 cGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGtt                                  >  4:719709/1‑150 (MQ=255)
                                                                                                                 cGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTc                                 >  3:214330/1‑151 (MQ=255)
                                                                                                                  ggctggctGGCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCt                                <  2:1225402/151‑1 (MQ=255)
                                                                                                                    ctggctggCGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTgg                              >  3:167670/1‑151 (MQ=255)
                                                                                                                     tggctggcGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTGGt                             >  4:1278850/1‑151 (MQ=255)
                                                                                                                      ggctggcGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTgg                              <  2:1565910/149‑1 (MQ=255)
                                                                                                                         tggcGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGGCTAACTTCTGGCACTATTTTTTTTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTGGtact                          >  2:1026656/1‑150 (MQ=255)
                                                                                                                          ggcGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTGGtact                          <  2:1102796/149‑1 (MQ=255)
                                                                                                                            cGGTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTGGTACTACGt                      >  4:1303055/1‑151 (MQ=255)
                                                                                                                              gTTATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTGGTACTACGTGc                    <  4:409557/151‑1 (MQ=255)
                                                                                                                                tATCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTGGTACTACGTGCCg                  >  2:851407/1‑151 (MQ=255)
                                                                                                                                 aTCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTGGTACTACGTGCCgg                 >  2:552581/1‑151 (MQ=255)
                                                                                                                                 aTCAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTGGTACTACGTGCCg                  <  1:237427/150‑1 (MQ=255)
                                                                                                                                   cAGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTGGTACTACGTGCCGGtc               >  2:717698/1‑151 (MQ=255)
                                                                                                                                    aGTTGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTGGTACTACGTGCCGGtca              <  2:342192/151‑1 (MQ=255)
                                                                                                                                       tGCGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTGGTACTACGTGCCGGtcatc            >  4:736462/1‑150 (MQ=255)
                                                                                                                                         cGTACTGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGTACTGGATGATGCCCAACATAGAGCTCCGTTCTGGTACTACGTGCCGGTCATCAtt         <  4:1002071/151‑1 (MQ=255)
                                                                                                                                              tGACGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTGGTACTACGTGCCGGTCATCATTGCCgg    >  4:1038503/1‑151 (MQ=255)
                                                                                                                                                aCGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTGGTACTACGTGCCGGTCATCATTGCCGGt   >  2:30528/1‑150 (MQ=255)
                                                                                                                                                aCGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTGGTACTACGTGCCGGTCATCATTGCCGGTa  <  4:1295343/151‑1 (MQ=255)
                                                                                                                                                aCGGCTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTGGTACTACGTGCCGGTCATCATTGCCGGTa  <  2:311541/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ATCACCTGCGGCATGGGGGTGATGACCAAAGGTTTTCTCGCCCTTGCCGTGCCGGTATTAAGCGTGCTGCCATGGGTAGCGACGCAAAAACGCTGGAAAGATCTCTTTATTTACGGCTGGCTGGCGGTTATCAGTTGCGTACTGACGGTTCTCCCTTGGGGACTGGCGATAGCGCAGCGGGAGCCTAACTTCTGGCACTATTTTTTCTGGGTTGAGCATATTCAACGCTTTGCACTGGATGATGCCCAACATAGAGCTCCGTTCTGGTACTACGTGCCGGTCATCATTGCCGGTA  >  NC_000913/2371415‑2371709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: