Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,373,305	T→C	E78E (GAA→GAG)	pmrD ←	inactive two‑component system connector protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,373,305	0	T	C	100.0%	365.0 / NA	99	E78E (GAA→GAG)	pmrD	inactive two‑component system connector protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/54);  total (45/54)

CAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTCGGCGATCATT  >  NC_000913/2373163‑2373452                                                                                                                                               |                                                                                                                                                    cAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACt                                                                                                                                              <  4:1230723/150‑1 (MQ=255) cAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTc                                                                                                                                             >  4:993340/1‑151 (MQ=255) cAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTc                                                                                                                                             >  2:1170526/1‑151 (MQ=255)  aaTGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCa                                                                                                                                            <  2:388383/151‑1 (MQ=255)   aTGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCAt                                                                                                                                           >  2:1632863/1‑151 (MQ=255)   aTGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCAt                                                                                                                                           <  4:154648/151‑1 (MQ=255)      gTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCgg                                                                                                                                        <  4:1200756/151‑1 (MQ=255)          ttCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCt                                                                                                                                    >  4:1664901/1‑151 (MQ=255)               ggttGGAAAGGATCTTTTTCGCTGAAAACACTACTGGGAGTACCCTGTATTGTGAGCGGTTTGATGCTGATTTTCCTCCCCACGACAAAAAAATGTTGCCGAGTTTCCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAAc                                                                                                                               <  2:633582/148‑1 (MQ=255)                  tGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGc                                                                                                                            >  1:420921/1‑151 (MQ=255)                   gggAAGGGACTTTTCCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTCCTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTg                                                                                                                             <  2:520688/149‑1 (MQ=255)                     gAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTg                                                                                                                         >  2:54794/1‑151 (MQ=255)                      aaGGGACTTTTTCGCTGAAAGCACTACGGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGAAAAAACACCGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTg                                                                                                                         <  4:1656258/150‑1 (MQ=255)                        gggACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCg                                                                                                                      >  2:172610/1‑151 (MQ=255)                          gACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCgag                                                                                                                    <  4:236081/151‑1 (MQ=255)                            cTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCgag                                                                                                                    >  2:545563/1‑149 (MQ=255)                                 tagCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACtt                                                                                                             >  2:1562373/3‑151 (MQ=255)                                    cTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTc                                                                                                           <  3:186135/150‑1 (MQ=255)                                     tGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTc                                                                                                           <  4:211610/149‑1 (MQ=255)                                      gAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCAcc                                                                                                        >  2:1555259/1‑151 (MQ=255)                                       aaaGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCg                                                                                                       >  2:856435/1‑151 (MQ=255)                                        aaGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGt                                                                                                      <  2:1535698/151‑1 (MQ=255)                                          gCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTAt                                                                                                    <  2:128949/151‑1 (MQ=255)                                           cACTACTGGGAGTAGCCTGTATTATGAGCGGTTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACGTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTAt                                                                                                    <  3:377219/150‑1 (MQ=255)                                           cACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGATTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTAt                                                                                                    <  1:138872/150‑1 (MQ=255)                                           cACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTAt                                                                                                    <  3:63263/150‑1 (MQ=255)                                           cACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATg                                                                                                   >  2:907860/1‑151 (MQ=255)                                            actactGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGc                                                                                                  >  4:243075/1‑151 (MQ=255)                                            actactGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGc                                                                                                  >  2:587561/1‑151 (MQ=255)                                              tactGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGc                                                                                                  >  4:80599/1‑149 (MQ=255)                                               actGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGc                                                                                               >  3:173981/1‑151 (MQ=255)                                                ctGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAg                                                                                                <  4:916599/149‑1 (MQ=255)                                                ctGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGc                                                                                               <  4:1009686/150‑1 (MQ=255)                                                  gggAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACTTTACTGATTTTCCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCt                                                                                              <  4:1628171/149‑1 (MQ=255)                                                  gggAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCttt                                                                                            >  2:1256064/1‑151 (MQ=255)                                                   ggAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCtttt                                                                                           <  4:271509/151‑1 (MQ=255)                                                   ggAGTAGCCTATATTATGAGCGGGTGTATGCTGATTTCCCTGCCCACGACAAAACAACGTTCCTGATTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGc                                                                                               <  2:1316731/147‑1 (MQ=255)                                                    gAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTc                                                                                          <  4:96173/151‑1 (MQ=255)                                                       tAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCAc                                                                                        <  4:1342353/150‑1 (MQ=255)                                                        aGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACg                                                                                       >  1:76291/1‑150 (MQ=255)                                                          ccTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGAt                                                                                     <  1:229325/150‑1 (MQ=255)                                                          ccTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGAt                                                                                     <  2:1306535/150‑1 (MQ=255)                                                            tGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTa                                                                                   >  4:1413338/1‑150 (MQ=255)                                                                ttatGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATAc                                                                               <  4:928048/150‑1 (MQ=255)                                                                 tatGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACa                                                                              <  4:1620666/150‑1 (MQ=255)                                                                   tGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTCTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAAt                                                                            >  2:736853/1‑150 (MQ=255)                                                                    gAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATa                                                                           >  3:115101/1‑150 (MQ=255)                                                                      gCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATaaa                                                                         <  4:1449074/150‑1 (MQ=255)                                                                       cGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAgc                                                                       >  2:894474/1‑151 (MQ=255)                                                                        gggTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAgc                                                                       <  4:207368/150‑1 (MQ=255)                                                                        gggTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAgc                                                                       <  1:332612/150‑1 (MQ=255)                                                                         ggTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAgcgc                                                                     >  2:486832/1‑151 (MQ=255)                                                                          gTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAgcgc                                                                     >  3:366244/1‑150 (MQ=255)                                                                           ttGATCCTGATTTTCCTGCCCACGACAAAACAACGTTCCTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCa                                                                    <  1:88068/150‑1 (MQ=255)                                                                            tGATGCTGATTTTCCTGCCCACGACAAAACAAGGTTACTTGGTTTCCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCAt                                                                   <  2:989945/150‑1 (MQ=255)                                                                            tGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCAtt                                                                  >  4:1136048/1‑151 (MQ=255)                                                                                 cTGATTTTCCTCCCCCCGAAAAAACAACGTTACTGAGTTTCCCCTGCCACTTTACACTGCCGCTCCCACTCATCGTGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTg                                                               <  1:441157/149‑1 (MQ=255)                                                                                  tGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCa                                                             >  4:447091/1‑150 (MQ=255)                                                                                     ttttCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAg                                                          <  4:289399/150‑1 (MQ=255)                                                                                     ttttCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAg                                                          <  2:365849/150‑1 (MQ=255)                                                                                        tCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCg                                                       >  4:1166485/1‑150 (MQ=255)                                                                                             cccACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCgaga                                                    <  2:1210411/148‑1 (MQ=255)                                                                                                 cGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGTGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATc                                             >  1:463196/1‑151 (MQ=255)                                                                                                 cGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATc                                             <  3:133034/151‑1 (MQ=255)                                                                                                 cGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATc                                             <  4:664959/151‑1 (MQ=255)                                                                                                  gACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATcc                                            <  3:167482/151‑1 (MQ=255)                                                                                                  gACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATcc                                            >  2:168111/1‑151 (MQ=255)                                                                                                  gACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATcc                                            >  2:28713/1‑151 (MQ=255)                                                                                                   aCAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATccc                                           >  2:558290/1‑151 (MQ=255)                                                                                                     aaaaCAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATccccc                                         <  3:381801/151‑1 (MQ=255)                                                                                                     aaaaCAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATccccc                                         <  2:62345/151‑1 (MQ=255)                                                                                                       aacaacGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCAc                                       >  4:1475105/1‑151 (MQ=255)                                                                                                        acaacGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACt                                      >  2:1044758/1‑151 (MQ=255)                                                                                                         caacGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACtt                                     >  3:404149/1‑151 (MQ=255)                                                                                                          aacGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACttt                                    <  2:514822/151‑1 (MQ=255)                                                                                                           acGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTc                                   >  4:857773/1‑151 (MQ=255)                                                                                                            cGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACttt                                    <  4:626578/149‑1 (MQ=255)                                                                                                                 cTGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACgg                               <  2:242687/149‑1 (MQ=255)                                                                                                                  tGAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCg                             >  4:1329269/1‑150 (MQ=255)                                                                                                                   gAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCg                             <  4:1381210/149‑1 (MQ=255)                                                                                                                   gAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGaa                           <  4:450987/151‑1 (MQ=255)                                                                                                                   gAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGaa                           <  4:474903/151‑1 (MQ=255)                                                                                                                   gAGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGa                            <  4:1557182/150‑1 (MQ=255)                                                                                                                    aGTTTTCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCg                             <  3:83006/148‑1 (MQ=255)                                                                                                                        ttCCCTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCa                       <  2:1208983/150‑1 (MQ=255)                                                                                                                           ccTGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCActct                   >  4:989803/1‑151 (MQ=255)                                                                                                                             tGCCACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTc                 >  4:407590/1‑151 (MQ=255)                                                                                                                               ccACTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTCAc               <  4:371964/151‑1 (MQ=255)                                                                                                                                  cTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTc            >  1:450636/1‑151 (MQ=255)                                                                                                                                  cTTTACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTc            >  3:430299/1‑151 (MQ=255)                                                                                                                                   tttACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTCg           >  2:237497/1‑151 (MQ=255)                                                                                                                                    ttACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTc            <  4:277499/149‑1 (MQ=255)                                                                                                                                    ttACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTc            <  3:98195/149‑1 (MQ=255)                                                                                                                                    ttACACTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTCg           <  4:405677/150‑1 (MQ=255)                                                                                                                                       cacTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTCGGCg        >  2:1461841/1‑150 (MQ=255)                                                                                                                                        acTGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTCGGCGAt      >  4:683424/1‑151 (MQ=255)                                                                                                                                          tGCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTCGGCGATc     >  4:89369/1‑150 (MQ=255)                                                                                                                                           gCCGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTCGGCGATc     <  2:446457/149‑1 (MQ=255)                                                                                                                                            ccGCTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTCGGCGATCAtt  >  3:15126/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                                    CAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCTATAACTGCTTGCCGAGAGGACTTTCACCGTATGCAGCTTTTCACGATTAATACAATAAAGCGCATTCTGCAAAGGCGAGAGTAAATCCCCCACTTTCACGGCGAAGTCACTCTTCACTTCGGCGATCATT  >  NC_000913/2373163‑2373452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑