BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,484,572	A→G	T67A (ACG→GCG)	evgS →	hybrid sensory histidine kinase in two‑component regulatory system with EvgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,484,572	0	A	G	100.0%	452.0 / NA	118	T67A (ACG→GCG)	evgS	hybrid sensory histidine kinase in two‑component regulatory system with EvgA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (44/74); total (44/74)

Only 100 of 118 total aligned reads displayed.

CATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCA  >  NC_000913/2484424‑2484715

cATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCg                                                                                                                                               <  4:874548/151‑1 (MQ=255)

 aTAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGC‑AAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGc                                                                                                                                             <  3:182198/151‑1 (MQ=255)

   aaGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGc                                                                                                                                             <  4:36425/150‑1 (MQ=255)

        tCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACgtt                                                                                                                                       >  4:149311/1‑151 (MQ=255)

        tCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACgtt                                                                                                                                       >  2:845198/1‑151 (MQ=255)

         cGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACgttg                                                                                                                                      >  2:430850/1‑151 (MQ=255)

         cGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACgttg                                                                                                                                      <  4:611646/151‑1 (MQ=255)

              aCGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGc                                                                                                                                  >  4:1235383/1‑150 (MQ=255)

              aCGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCa                                                                                                                                 <  2:96950/151‑1 (MQ=255)

                gAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATa                                                                                                                               <  4:1280542/151‑1 (MQ=255)

                        cATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCg                                                                                                                       >  4:1069085/1‑151 (MQ=255)

                        cATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCg                                                                                                                       >  2:143357/1‑151 (MQ=255)

                        cATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCg                                                                                                                       <  1:182248/151‑1 (MQ=255)

                         aTCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCgc                                                                                                                      >  2:1288063/1‑151 (MQ=255)

                           cGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCgcag                                                                                                                    <  4:310009/151‑1 (MQ=255)

                            gAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCgcagc                                                                                                                   >  2:1366124/1‑151 (MQ=255)

                             aaTATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCgcagca                                                                                                                  <  2:1273693/151‑1 (MQ=255)

                               tatCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAAc                                                                                                                >  4:608266/1‑151 (MQ=255)

                                atCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAAc                                                                                                                <  2:136848/150‑1 (MQ=255)

                                atCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACg                                                                                                               <  2:1569701/151‑1 (MQ=255)

                                  cGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAAc                                                                                                                <  4:832873/148‑1 (MQ=255)

                                  cGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACg                                                                                                               <  2:72070/149‑1 (MQ=255)

                                    tGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGtt                                                                                                           >  1:350471/1‑151 (MQ=255)

                                     ggCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTc                                                                                                          <  1:292935/151‑1 (MQ=255)

                                     ggCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTc                                                                                                          <  2:449451/151‑1 (MQ=255)

                                      gCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCg                                                                                                         >  3:70348/1‑151 (MQ=255)

                                        aTCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTg                                                                                                       >  2:1226055/1‑151 (MQ=255)

                                          cAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGt                                                                                                     >  1:258344/1‑151 (MQ=255)

                                           agtagtAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTa                                                                                                    <  4:1683428/151‑1 (MQ=255)

                                           agtagtAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTa                                                                                                    <  2:399892/151‑1 (MQ=255)

                                              agtaACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTTTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTa                                                                                                 <  1:184659/151‑1 (MQ=255)

                                                 aacaacCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATg                                                                                              >  2:296217/1‑151 (MQ=255)

                                                  acaacCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGc                                                                                             <  4:463563/151‑1 (MQ=255)

                                                   caacCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCt                                                                                            <  1:353154/151‑1 (MQ=255)

                                                    aacCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTg                                                                                           >  2:952316/1‑151 (MQ=255)

                                                     acCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTg                                                                                           >  1:375905/1‑150 (MQ=255)

                                                          gtCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGattat                                                                                      <  2:531678/150‑1 (MQ=255)

                                                          gtCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGattat                                                                                      <  2:212260/150‑1 (MQ=255)

                                                            cacaCTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTAttt                                                                                    <  3:96232/150‑1 (MQ=255)

                                                            cacaCTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTAttt                                                                                    <  2:884234/150‑1 (MQ=255)

                                                              cacTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTaa                                                                                  <  4:1413698/150‑1 (MQ=255)

                                                               acTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTATATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAAt                                                                                <  3:351369/151‑1 (MQ=255)

                                                               acTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAAt                                                                                <  4:1277082/151‑1 (MQ=255)

                                                                 ttGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCt                                                                              >  4:810187/1‑151 (MQ=255)

                                                                  tGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCt                                                                              <  4:134056/150‑1 (MQ=255)

                                                                   gATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCttt                                                                            <  2:185565/151‑1 (MQ=255)

                                                                   gATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCttt                                                                            >  2:57801/1‑151 (MQ=255)

                                                                      ccACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTaa                                                                         <  4:1443165/151‑1 (MQ=255)

                                                                      ccACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTaa                                                                         <  1:336631/151‑1 (MQ=255)

                                                                      ccACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTa                                                                          <  4:444419/150‑1 (MQ=255)

                                                                        aCTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTaaaa                                                                       <  2:1011876/151‑1 (MQ=255)

                                                                         cTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTaaaa                                                                       >  4:1294271/1‑150 (MQ=255)

                                                                          tACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTaaaa                                                                       >  4:219838/1‑149 (MQ=255)

                                                                           aCGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTaaaaa                                                                      <  4:1310018/149‑1 (MQ=255)

                                                                           aCGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTaaaa                                                                       >  2:845214/1‑148 (MQ=255)

                                                                             gTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGc                                                                  >  3:84443/1‑151 (MQ=255)

                                                                                 gAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCg                                                                 <  4:1417565/148‑1 (MQ=255)

                                                                                 gAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGt                                                                <  3:350488/149‑1 (MQ=255)

                                                                                  aGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTaa                                                             <  4:281180/151‑1 (MQ=255)

                                                                                    caacaaGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAat                                                           >  2:1430079/1‑151 (MQ=255)

                                                                                    caacaaGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAat                                                           <  4:1367826/151‑1 (MQ=255)

                                                                                     aacaaGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAata                                                          <  4:16127/151‑1 (MQ=255)

                                                                                      acaaGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAatat                                                         <  4:431451/151‑1 (MQ=255)

                                                                                        aaGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATc                                                        >  4:143629/1‑150 (MQ=255)

                                                                                          ggAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCaaa                                                     <  3:312203/151‑1 (MQ=255)

                                                                                            aaTTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAAtt                                                   <  2:935906/151‑1 (MQ=255)

                                                                                                aCGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAac                                                <  4:1625/150‑1 (MQ=255)

                                                                                                  gTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACt                                             >  4:1106909/1‑151 (MQ=255)

                                                                                                  gTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACt                                             <  2:1265259/151‑1 (MQ=255)

                                                                                                  gTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACt                                             <  4:296675/151‑1 (MQ=255)

                                                                                                     ggTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTcc                                           <  4:1549365/150‑1 (MQ=255)

                                                                                                        tAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCggg                                        <  4:1106496/150‑1 (MQ=255)

                                                                                                        tAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCggg                                        <  2:344411/150‑1 (MQ=255)

                                                                                                         aGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGa                                       <  2:520361/150‑1 (MQ=255)

                                                                                                         aGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGa                                       >  2:513566/1‑150 (MQ=255)

                                                                                                            gAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATAc                                   <  3:163194/151‑1 (MQ=255)

                                                                                                             aGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACg                                  >  2:1556714/1‑151 (MQ=255)

                                                                                                               cAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGc                                 >  2:310473/1‑150 (MQ=255)

                                                                                                                  aaaaaaTCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGc                                 >  1:184965/1‑147 (MQ=255)

                                                                                                                   aaaaaTCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGc                                 >  4:464688/1‑146 (MQ=255)

                                                                                                                     aaaTCTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGatcat                          <  4:840939/151‑1 (MQ=255)

                                                                                                                         cTTGTGATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCaa                       >  4:798446/1‑150 (MQ=255)

                                                                                                                           tgtgATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGAtcatca                        <  3:54560/147‑1 (MQ=255)

                                                                                                                           tgtgATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCaaaa                     >  3:455521/1‑150 (MQ=255)

                                                                                                                            gtgATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCaaaaa                    <  3:28332/150‑1 (MQ=255)

                                                                                                                              gATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGc                  <  4:358362/150‑1 (MQ=255)

                                                                                                                              gATTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCa                 <  1:250512/151‑1 (MQ=255)

                                                                                                                               aTTGCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCaa                <  2:192198/151‑1 (MQ=255)

                                                                                                                                  gCAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATgg             <  2:887376/151‑1 (MQ=255)

                                                                                                                                   cAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGa            <  4:335506/151‑1 (MQ=255)

                                                                                                                                   cAGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGa            <  4:953565/151‑1 (MQ=255)

                                                                                                                                    aGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGAc           >  2:1143648/1‑151 (MQ=255)

                                                                                                                                    aGTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGAc           >  2:945949/1‑151 (MQ=255)

                                                                                                                                     gTACATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGAcg          >  4:49469/1‑151 (MQ=255)

                                                                                                                                       aCATAAGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGAcgcg        <  4:577582/151‑1 (MQ=255)

                                                                                                                                           aaGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCtt     <  4:90946/150‑1 (MQ=255)

                                                                                                                                            aGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGc   >  4:1141459/1‑151 (MQ=255)

                                                                                                                                            aGTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGc   <  1:38748/151‑1 (MQ=255)

                                                                                                                                             gTCCCAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCa  <  3:258359/151‑1 (MQ=255)

                                                                                                                                               cccAAGCGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGAATTAATGCTGAATATTTAAATCTTTTAAAAAGAGCGTTAACTATCAAATTAACACTCCGTAAATACGCAGATCATCAAAAAGCAATGGACGCGCTTg    >  4:787747/1‑147 (MQ=255)

CATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTACGTTGTTGCATACCGATTCGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCA  >  NC_000913/2484424‑2484715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`