Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,490,592	A→G	S453S (AGT→AGC)	oxc ←	oxalyl CoA decarboxylase, ThDP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,490,592	0	A	G	100.0%	359.4 / NA	97	S453S (AGT→AGC)	oxc	oxalyl CoA decarboxylase, ThDP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (54/43);  total (54/43)

CATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCAATAATA  >  NC_000913/2490444‑2490739                                                                                                                                                     |                                                                                                                                                    cATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCt                                                                                                                                                   <  4:833244/151‑1 (MQ=255)    aCCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGa                                                                                                                                                 >  2:377250/1‑150 (MQ=255)      cTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAAc                                                                                                                                              >  2:1536100/1‑151 (MQ=255)        tGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAAcc                                                                                                                                             <  2:1113347/150‑1 (MQ=255)         gCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCaa                                                                                                                                           <  4:836625/151‑1 (MQ=255)         gCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCa                                                                                                                                            <  4:976437/150‑1 (MQ=255)           aTGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCa                                                                                                                                            <  4:785509/148‑1 (MQ=255)              gTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGc                                                                                                                                       >  4:1383383/1‑150 (MQ=255)              gTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCa                                                                                                                                      <  4:99264/151‑1 (MQ=255)               tGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCAc                                                                                                                                     >  3:315638/1‑151 (MQ=255)                gCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCAc                                                                                                                                     <  4:1251771/150‑1 (MQ=255)                  aaCAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTAt                                                                                                                                  <  4:1315578/151‑1 (MQ=255)                  aaCAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTAt                                                                                                                                  >  2:1264488/1‑151 (MQ=255)                    cAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATc                                                                                                                                 >  4:612263/1‑150 (MQ=255)                         ccGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCtt                                                                                                                            <  3:436109/150‑1 (MQ=255)                           gTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCa                                                                                                                          >  4:1171341/1‑150 (MQ=255)                           gTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCa                                                                                                                          >  2:1061202/1‑150 (MQ=255)                             tGGTGATGGTGCACCAGCGCCACTGAGATCAATACCGTCTCCTCTGTAGATGCCGACATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAAAAAAAAGCACTATCACCTTCAAt                                                                                                                        >  2:952646/1‑150 (MQ=255)                              ggTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATg                                                                                                                       <  2:509559/150‑1 (MQ=255)                               gTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGc                                                                                                                     <  4:679002/151‑1 (MQ=255)                                    ggTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACa                                                                                                                 <  4:929604/150‑1 (MQ=255)                                       gCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAAcc                                                                                                              <  2:1335881/150‑1 (MQ=255)                                        cACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCgg                                                                                                            >  2:1474846/1‑151 (MQ=255)                                               gcCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCa                                                                                                     <  4:1643749/151‑1 (MQ=255)                                                ccACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAAcc                                                                                                      >  4:313382/1‑149 (MQ=255)                                                  aCTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGa                                                                                                   >  2:1497323/1‑150 (MQ=255)                                                    tGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGt                                                                                                >  3:444739/1‑151 (MQ=255)                                                     gagaTCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTc                                                                                               <  2:686533/151‑1 (MQ=255)                                                     gagaTCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTc                                                                                               >  4:1055492/1‑151 (MQ=255)                                                         tCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGc                                                                                           >  4:679866/1‑151 (MQ=255)                                                         tCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGc                                                                                           >  2:496528/1‑151 (MQ=255)                                                             cacCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAg                                                                                        <  4:43991/150‑1 (MQ=255)                                                             cacCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTGCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAg                                                                                        <  3:72920/150‑1 (MQ=255)                                                              acCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGc                                                                                       >  4:29408/1‑150 (MQ=255)                                                                cGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCAcc                                                                                    >  4:954224/1‑151 (MQ=255)                                                                cGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCAcc                                                                                    <  4:1436119/151‑1 (MQ=255)                                                                     cctcTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGAt                                                                                 <  1:444720/149‑1 (MQ=255)                                                                     cctcTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGAt                                                                                 <  2:411428/149‑1 (MQ=255)                                                                       tctGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGc                                                                              >  3:53857/1‑150 (MQ=255)                                                                        ctGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGc                                                                              <  2:266143/149‑1 (MQ=255)                                                                        ctGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCAt                                                                            >  3:346267/1‑151 (MQ=255)                                                                         tGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCAt                                                                            >  2:1589985/1‑150 (MQ=255)                                                                          gTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCa                                                                             >  2:560879/1‑148 (MQ=255)                                                                          gTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATa                                                                           <  2:46316/150‑1 (MQ=255)                                                                           tAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGc                                                                         >  4:1458373/1‑151 (MQ=255)                                                                            aGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGcc                                                                        <  1:317030/151‑1 (MQ=255)                                                                            aGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGcc                                                                        >  4:1094046/1‑151 (MQ=255)                                                                               tGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCAt                                                                     >  2:273058/1‑151 (MQ=255)                                                                                  cgccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATAcc                                                                  >  1:191707/1‑151 (MQ=255)                                                                                    ccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCg                                                                 >  2:1382651/1‑150 (MQ=255)                                                                                        tattaAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGcc                                                            >  4:1609021/1‑151 (MQ=255)                                                                                         attaAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGccc                                                           >  2:237889/1‑151 (MQ=255)                                                                                         attaAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGccc                                                           >  4:626634/1‑151 (MQ=255)                                                                                         attaAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGccc                                                           >  3:95519/1‑151 (MQ=255)                                                                                          ttaAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGccc                                                           >  2:67036/1‑150 (MQ=255)                                                                                           taAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCa                                                          <  2:1210868/150‑1 (MQ=255)                                                                                           taAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCAt                                                         >  3:428003/1‑151 (MQ=255)                                                                                                aTAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGacac                                                    >  4:1513418/1‑151 (MQ=255)                                                                                                   aCGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACAcccc                                                 >  2:1347314/1‑151 (MQ=255)                                                                                                    cGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACAcccc                                                 >  2:1636068/1‑150 (MQ=255)                                                                                                       tCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCAt                                                                     <  3:79300/127‑1 (MQ=255)                                                                                                       tCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCAt                                                                     >  1:79296/1‑127 (MQ=255)                                                                                                                   ggTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATcc                                   >  2:424891/1‑149 (MQ=255)                                                                                                                   ggTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATcc                                   >  2:719769/1‑149 (MQ=255)                                                                                                                   ggTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAg                                 <  4:8345/151‑1 (MQ=255)                                                                                                                    gTTATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAga                                <  2:1169862/151‑1 (MQ=255)                                                                                                                     ttATATCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAga                                >  1:11583/1‑150 (MQ=255)                                                                                                                      tataTCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTGCCACAATCCAgac                               <  2:1076413/150‑1 (MQ=255)                                                                                                                       atatCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacg                              >  2:1023032/1‑150 (MQ=255)                                                                                                                         atCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacg                           >  4:684488/1‑151 (MQ=255)                                                                                                                         atCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacg                           <  4:5651/151‑1 (MQ=255)                                                                                                                         atCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgac                            <  2:730682/150‑1 (MQ=255)                                                                                                                         atCGACAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgac                            <  4:1106053/150‑1 (MQ=255)                                                                                                                            gACAAATCGTTTCAATTTCCATCCCGCTGAACCCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCGCAATCCAgacgacgac                         <  4:38055/150‑1 (MQ=255)                                                                                                                             aCAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGt                       >  4:768387/1‑151 (MQ=255)                                                                                                                              cAAATCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTg                      <  4:1677807/151‑1 (MQ=255)                                                                                                                               aaaTCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTgg                     <  4:130686/151‑1 (MQ=255)                                                                                                                                 aTCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGtt                   >  4:937829/1‑151 (MQ=255)                                                                                                                                 aTCGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGtt                   <  4:1538009/151‑1 (MQ=255)                                                                                                                                   cGTTTCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTta                 <  4:1450726/151‑1 (MQ=255)                                                                                                                                     tttCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTtat                >  3:231350/1‑150 (MQ=255)                                                                                                                                     tttCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGACGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTtat                >  2:811568/1‑150 (MQ=255)                                                                                                                                      ttCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTtata               <  2:628819/150‑1 (MQ=255)                                                                                                                                      ttCAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATAc              >  2:1256691/1‑151 (MQ=255)                                                                                                                                        cAATTTCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACat            >  3:135854/1‑151 (MQ=255)                                                                                                                                           tttCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATc         >  4:752933/1‑151 (MQ=255)                                                                                                                                           tttCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATc         >  2:535407/1‑151 (MQ=255)                                                                                                                                            ttCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATc         <  3:2518/150‑1 (MQ=255)                                                                                                                                             tCCATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCa        >  4:893416/1‑150 (MQ=255)                                                                                                                                               cATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCaata     <  4:429833/151‑1 (MQ=255)                                                                                                                                               cATCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCaat      >  4:107381/1‑150 (MQ=255)                                                                                                                                                aTCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCaataa    <  4:1572255/151‑1 (MQ=255)                                                                                                                                                aTCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCaataa    <  2:1363965/151‑1 (MQ=255)                                                                                                                                                aTCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCaata     >  4:384026/1‑150 (MQ=255)                                                                                                                                                 tCCCGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCaataat   >  3:423112/1‑151 (MQ=255)                                                                                                                                                  cccGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCaataat   <  4:918667/150‑1 (MQ=255)                                                                                                                                                  cccGCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCAataata  <  2:360638/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCAATAATA  >  NC_000913/2490444‑2490739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑