Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 257,644 A→G D373G (GAC→GGC)  frsA → fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913257,6440AG99.2% 439.7 / ‑4.4 118D373G (GAC→GGC) frsAfermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (61/56);  total (62/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 118 total aligned reads displayed.

CGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTT  >  NC_000913/257496‑257787
                                                                                                                                                    |                                                                                                                                               
cGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCt                                                                                                                                               >  4:1450720/1‑151 (MQ=255)
 gTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCt                                                                                                                                               <  4:106183/150‑1 (MQ=255)
 gTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTc                                                                                                                                              >  4:435024/1‑151 (MQ=255)
 gTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTc                                                                                                                                              >  4:457008/1‑151 (MQ=255)
   ttGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCa                                                                                                                                             >  2:1087336/1‑150 (MQ=255)
     ggggATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGc                                                                                                                                          >  2:364486/1‑151 (MQ=255)
      gggATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCt                                                                                                                                         >  2:946712/1‑151 (MQ=255)
       ggATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCtt                                                                                                                                        <  4:223399/151‑1 (MQ=255)
          tGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAAt                                                                                                                                     >  3:331231/1‑151 (MQ=255)
          tGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAAt                                                                                                                                     >  4:65672/1‑151 (MQ=255)
          tGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAAt                                                                                                                                     >  4:1442756/1‑151 (MQ=255)
            cATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCa                                                                                                                                   <  2:1147787/151‑1 (MQ=255)
             atgatgCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCAc                                                                                                                                  <  2:337863/151‑1 (MQ=255)
               gatgCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCt                                                                                                                                >  2:423872/1‑151 (MQ=255)
                    ttCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCtcatc                                                                                                                            >  4:1147982/1‑150 (MQ=255)
                     tCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTcatcat                                                                                                                          >  2:1665835/1‑151 (MQ=255)
                       cGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATct                                                                                                                        >  4:94651/1‑151 (MQ=255)
                           gAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATctgctg                                                                                                                    >  4:538968/1‑151 (MQ=255)
                              gCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACg                                                                                                                 <  2:1198002/151‑1 (MQ=255)
                              gCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACg                                                                                                                 >  4:1094031/1‑151 (MQ=255)
                                gTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGt                                                                                                               <  1:458363/151‑1 (MQ=255)
                                 ttGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGt                                                                                                               >  2:1003630/1‑150 (MQ=255)
                                      cgTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAatta                                                                                                         <  4:1077647/151‑1 (MQ=255)
                                      cgTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAatt                                                                                                          >  2:868373/1‑150 (MQ=255)
                                         ggAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAattatt                                                                                                       >  4:864207/1‑150 (MQ=255)
                                         ggAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAattatt                                                                                                       <  4:1539733/150‑1 (MQ=255)
                                          gAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAattatt                                                                                                       >  4:1365271/1‑149 (MQ=255)
                                          gAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTag                                                                                                     <  4:1682810/151‑1 (MQ=255)
                                           aGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTaga                                                                                                    <  3:276316/151‑1 (MQ=255)
                                            gCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTaga                                                                                                    <  2:1246147/150‑1 (MQ=255)
                                              tGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTag                                                                                                     >  1:338118/1‑147 (MQ=255)
                                               gAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGAt                                                                                                 <  2:1080825/150‑1 (MQ=255)
                                               gAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGAt                                                                                                 <  4:1558761/150‑1 (MQ=255)
                                                aaTCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATcc                                                                                               >  2:292695/1‑151 (MQ=255)
                                                   cGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCa                                                                                             <  4:1019936/150‑1 (MQ=255)
                                                      tATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCAttt                                                                                          >  1:409184/1‑150 (MQ=255)
                                                      tATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCAttt                                                                                          >  2:245430/1‑150 (MQ=255)
                                                       aTTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTa                                                                                         >  3:108523/1‑150 (MQ=255)
                                                           aTTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCg                                                                                    >  2:443594/1‑151 (MQ=255)
                                                           aTTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCg                                                                                    >  2:1531902/1‑151 (MQ=255)
                                                             tAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCgg                                                                                   <  4:184368/150‑1 (MQ=255)
                                                             tAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGgt                                                                                  >  3:312560/1‑151 (MQ=255)
                                                             tAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGgt                                                                                  >  2:1222160/1‑151 (MQ=255)
                                                              aaaaGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGgtg                                                                                 <  4:1111121/151‑1 (MQ=255)
                                                              aaaaGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGgtg                                                                                 <  4:819931/151‑1 (MQ=255)
                                                                  gTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATc                                                                             >  4:697119/1‑151 (MQ=255)
                                                                   tGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCg                                                                            >  2:366708/1‑151 (MQ=255)
                                                                     cAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCgg                                                                           >  2:1494483/1‑150 (MQ=255)
                                                                      aaGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGa                                                                          >  2:837409/1‑150 (MQ=255)
                                                                        ggATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAAtt                                                                       <  2:889749/151‑1 (MQ=255)
                                                                         gATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAAttt                                                                      >  4:1625408/1‑151 (MQ=255)
                                                                            tGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTg                                                                    >  4:1462058/1‑150 (MQ=255)
                                                                             gCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGa                                                                   <  1:30689/150‑1 (MQ=255)
                                                                             gCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGAc                                                                  >  4:408122/1‑151 (MQ=255)
                                                                              cTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGAc                                                                  >  2:795828/1‑150 (MQ=255)
                                                                              cTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGAc                                                                  >  4:590941/1‑150 (MQ=255)
                                                                                  gACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAgg                                                             >  4:1565332/1‑151 (MQ=255)
                                                                                   aCGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGt                                                            >  4:958967/1‑151 (MQ=255)
                                                                                     gTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCt                                                          >  2:571531/1‑151 (MQ=255)
                                                                                       cGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCtt                                                         <  2:144741/150‑1 (MQ=255)
                                                                                           gCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCTCCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAgg                                                     <  4:1311734/150‑1 (MQ=255)
                                                                                            cccAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGaa                                                   <  1:442024/151‑1 (MQ=255)
                                                                                             ccAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGaa                                                   >  2:643594/1‑150 (MQ=255)
                                                                                                   ccAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCg                                            <  4:1121959/151‑1 (MQ=255)
                                                                                                    cAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGa                                           <  3:54361/151‑1 (MQ=255)
                                                                                                     aaTGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGAc                                          >  2:965361/1‑151 (MQ=255)
                                                                                                        gTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTgg                                       <  4:306911/151‑1 (MQ=255)
                                                                                                        gTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTgg                                       >  4:1248191/1‑151 (MQ=255)
                                                                                                        gTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTg                                        <  4:424475/150‑1 (MQ=255)
                                                                                                         ttATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTgg                                       >  2:1452169/1‑150 (MQ=255)
                                                                                                          tataAGGCTACTGGAAGAACGATCAGTTCAGCCCGGCAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGAt                                     >  1:83109/1‑151 (MQ=255)
                                                                                                          tATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGa                                      <  2:434419/150‑1 (MQ=255)
                                                                                                           aTCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCGCCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAAGCACCGGCTGGATc                                    <  2:260839/151‑1 (MQ=255)
                                                                                                           aTCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGAt                                     <  4:590216/150‑1 (MQ=255)
                                                                                                           aTCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATc                                    >  4:447437/1‑151 (MQ=255)
                                                                                                           aTCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATc                                    <  2:407539/151‑1 (MQ=255)
                                                                                                             cAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGa                                  <  1:331214/151‑1 (MQ=255)
                                                                                                              aGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGaa                                 <  3:202291/151‑1 (MQ=255)
                                                                                                              aGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGaa                                 <  4:214336/151‑1 (MQ=255)
                                                                                                               ggCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGaaa                                <  2:1440956/151‑1 (MQ=255)
                                                                                                               ggCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGaaa                                <  2:1146526/151‑1 (MQ=255)
                                                                                                                   aCTGGAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGaaaaa                              <  4:1059303/149‑1 (MQ=255)
                                                                                                                      ggAAGAACGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCtt                         <  4:1563075/151‑1 (MQ=255)
                                                                                                                       gaagaaCGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTtg                        <  4:1222176/151‑1 (MQ=255)
                                                                                                                        aagaaCGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTtgt                       >  2:103753/1‑151 (MQ=255)
                                                                                                                        aagaaCGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTtgt                       <  3:424168/151‑1 (MQ=255)
                                                                                                                         agaaCGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTtgtg                      <  3:201067/151‑1 (MQ=255)
                                                                                                                           aaCGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGtt                    >  2:616777/1‑151 (MQ=255)
                                                                                                                           aaCGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGCAAATCACCGACTGGATCGAAAAACGCTTGTGtt                    <  4:513225/151‑1 (MQ=255)
                                                                                                                            aCGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGtt                    >  4:1518095/1‑150 (MQ=255)
                                                                                                                             cGATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTaa                  <  2:898382/151‑1 (MQ=255)
                                                                                                                              gATCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTaaa                 <  2:1448907/151‑1 (MQ=255)
                                                                                                                               aTCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTaaaa                <  2:570909/151‑1 (MQ=255)
                                                                                                                                tCCGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTaaaa                >  3:148049/1‑150 (MQ=255)
                                                                                                                                 ccGTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAAt              <  3:189078/151‑1 (MQ=255)
                                                                                                                                   gTTCAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAAttt            <  4:1088718/151‑1 (MQ=255)
                                                                                                                                      cAGCCCGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCt         >  4:913529/1‑151 (MQ=255)
                                                                                                                                           cGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAAtt    <  1:65269/151‑1 (MQ=255)
                                                                                                                                           cGGAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAAtt    <  4:1335773/151‑1 (MQ=255)
                                                                                                                                             gAAGAGGGCTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAAtttt  <  4:1667861/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CGTTTGGGGATGCATGATGCTTCCGATGAAGCGTTGCGCGTGGAGCTGAATCGCTATTCATTAAAAGTGCAAGGATTGCTGGGACGTCGCTGCCCAACGCCAATGTTATCAGGCTACTGGAAGAACGATCCGTTCAGCCCGGAAGAGGACTCACGCTTAATCACCTCATCATCTGCTGACGGTAAATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTT  >  NC_000913/257496‑257787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: