Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,550,463 T→C D28G (GAC→GGC)  yfeX ← porphyrinogen oxidase, cytoplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,550,4630TC100.0% 453.2 / NA 117D28G (GAC→GGC) yfeXporphyrinogen oxidase, cytoplasmic
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (53/64);  total (53/64)

Only 100 of 117 total aligned reads displayed.

TCAGCTCTTCTGCCCCAACGCCGCCGCTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTG  >  NC_000913/2550317‑2550607
                                                                                                                                                  |                                                                                                                                                
tCAGCTCTTCTGCCCCAACGCCGCCGCTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAAc                                                                                                                                              >  4:905436/1‑151 (MQ=255)
 cAGCTCTTCTGCCCCAACGCCGCCGCTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACt                                                                                                                                             <  2:947372/151‑1 (MQ=255)
      cttctGCCCCAACGCCGCCGCTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTcccc                                                                                                                                        >  2:876426/1‑151 (MQ=255)
      cttctGCCCCAACGCCGCCGCTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTcc                                                                                                                                          <  4:903735/149‑1 (MQ=255)
       ttctGCCCCAACGCCGCCGCTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCt                                                                                                                                       >  1:345588/1‑151 (MQ=255)
       ttctGCCCCAACGCCGCCGCTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCt                                                                                                                                       >  2:575773/1‑151 (MQ=255)
        tctGCCCCAACGCCGCCGCTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTcccc                                                                                                                                        <  4:1119364/149‑1 (MQ=255)
           gCCCCAACGCCGCCGCTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCtt                                                                                                                                      <  2:256871/148‑1 (MQ=255)
           gCCCCAACGCCGCCGCTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTc                                                                                                                                    >  4:811425/1‑150 (MQ=255)
            ccccAACGCCGCCGCTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCAc                                                                                                                                  <  2:420091/151‑1 (MQ=255)
             cccAACGCCGCCGCTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACg                                                                                                                                 >  4:593657/1‑151 (MQ=255)
             cccAACGCCGCCGCTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACg                                                                                                                                 >  3:137700/1‑151 (MQ=255)
                  cgccgccgcTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGc                                                                                                                            <  2:803289/151‑1 (MQ=255)
                     tgccgcTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTc                                                                                                                         >  2:1568159/2‑151 (MQ=255)
                       ccgcTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGa                                                                                                                       <  1:306089/151‑1 (MQ=255)
                        cgcTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGAt                                                                                                                      >  3:357456/1‑151 (MQ=255)
                         gcTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATc                                                                                                                     >  4:246107/1‑151 (MQ=255)
                         gcTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATc                                                                                                                     >  2:1287117/1‑151 (MQ=255)
                           tCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATcc                                                                                                                    >  2:353926/1‑150 (MQ=255)
                           tCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATcc                                                                                                                    >  2:899043/1‑150 (MQ=255)
                            cAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCaa                                                                                                                  <  2:65623/151‑1 (MQ=255)
                            cAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCa                                                                                                                   <  1:41279/150‑1 (MQ=255)
                               agCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATc                                                                                                               <  2:829830/151‑1 (MQ=255)
                                gcgcgcCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATcg                                                                                                              >  4:1422202/1‑151 (MQ=255)
                                gcgcgcCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATcg                                                                                                              >  4:1316656/1‑151 (MQ=255)
                                gcgcgcCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATcg                                                                                                              <  3:344641/151‑1 (MQ=255)
                                   cgcCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATcgccg                                                                                                           >  2:454956/1‑151 (MQ=255)
                                   cgcCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATcgccg                                                                                                           <  2:279007/151‑1 (MQ=255)
                                    gcCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATcgccgc                                                                                                          <  4:794580/151‑1 (MQ=255)
                                     ccAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCcgcg                                                                                                         <  2:1616558/151‑1 (MQ=255)
                                      cAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCcgcgc                                                                                                        <  2:605765/151‑1 (MQ=255)
                                       aGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCcgcgc                                                                                                        >  2:1425416/1‑150 (MQ=255)
                                          tgttgtTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGc                                                                                                     >  2:1355308/1‑150 (MQ=255)
                                            ttgttACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAAt                                                                                                  >  1:139337/1‑151 (MQ=255)
                                               ttACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGtt                                                                                               >  2:897209/1‑151 (MQ=255)
                                                tACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTc                                                                                              >  2:189487/1‑151 (MQ=255)
                                                 aCCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCt                                                                                             >  4:1515491/1‑151 (MQ=255)
                                                 aCCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCt                                                                                             <  2:307829/151‑1 (MQ=255)
                                                   cAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTgg                                                                                           >  3:430483/1‑151 (MQ=255)
                                                   cAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTgg                                                                                           <  2:285032/151‑1 (MQ=255)
                                                    aaaGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGc                                                                                          <  2:1239653/151‑1 (MQ=255)
                                                    aaaGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGc                                                                                          >  2:1516144/1‑151 (MQ=255)
                                                     aaGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGc                                                                                          >  2:121744/1‑150 (MQ=255)
                                                     aaGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGc                                                                                          >  4:382733/1‑150 (MQ=255)
                                                       ggCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCaaa                                                                                       <  2:172875/151‑1 (MQ=255)
                                                       ggCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCaaa                                                                                       <  1:366665/151‑1 (MQ=255)
                                                               ccGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCAct                                                                               >  1:111306/1‑151 (MQ=255)
                                                                 gCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCActct                                                                             <  4:1633756/151‑1 (MQ=255)
                                                                  cACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTg                                                                            <  3:301792/151‑1 (MQ=255)
                                                                  cACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGCTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTg                                                                            <  4:383231/151‑1 (MQ=255)
                                                                    ccAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGTGCGCCAACTTCCCCTGTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGac                                                                          <  1:47512/151‑2 (MQ=255)
                                                                     cAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAAc                                                                         <  1:367384/151‑1 (MQ=255)
                                                                           gcgcGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTgag                                                                    <  2:1524638/150‑1 (MQ=255)
                                                                            cgcgTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTgaga                                                                   >  4:435423/1‑150 (MQ=255)
                                                                            cgcgTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGAc                                                                  >  4:1325552/1‑151 (MQ=255)
                                                                               gTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACAtt                                                               >  2:1175051/1‑151 (MQ=255)
                                                                                      aaTTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTtcctc                                                        <  4:1089965/151‑1 (MQ=255)
                                                                                          tCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTg                                                     >  1:458644/1‑150 (MQ=255)
                                                                                          tCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTg                                                     >  4:203244/1‑150 (MQ=255)
                                                                                           cGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGa                                                    >  1:150004/1‑150 (MQ=255)
                                                                                           cGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGa                                                    <  2:1121839/150‑1 (MQ=255)
                                                                                                 aaaaGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAaataat                                             <  2:402001/151‑1 (MQ=255)
                                                                                                   aaGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAaataataa                                           <  2:904263/151‑1 (MQ=255)
                                                                                                   aaGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAaataata                                            >  4:1156425/1‑150 (MQ=255)
                                                                                                    aGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAaataataat                                          <  4:1105702/151‑1 (MQ=255)
                                                                                                    aGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAaataataat                                          <  2:1608512/151‑1 (MQ=255)
                                                                                                     gTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATg                                         <  1:113797/151‑1 (MQ=255)
                                                                                                     gTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATg                                         <  1:45424/151‑1 (MQ=255)
                                                                                                      ttGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGc                                        >  4:388552/1‑151 (MQ=255)
                                                                                                       tGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGc                                        <  2:825526/150‑1 (MQ=255)
                                                                                                        gCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCa                                      <  4:711449/151‑1 (MQ=255)
                                                                                                          cAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACg                                    <  1:450456/151‑1 (MQ=255)
                                                                                                           aGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGc                                   >  2:1619165/1‑151 (MQ=255)
                                                                                                           aGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGc                                   <  2:976963/151‑1 (MQ=255)
                                                                                                            gTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCa                                  <  4:67276/151‑1 (MQ=255)
                                                                                                            gTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCa                                  <  1:46517/151‑1 (MQ=255)
                                                                                                                gTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATcc                              >  4:1671046/1‑151 (MQ=255)
                                                                                                                gTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATc                               <  2:245719/150‑1 (MQ=255)
                                                                                                                  cGGCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGt                            <  4:1443429/151‑1 (MQ=255)
                                                                                                                   ggCAAATGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTg                           <  4:764570/151‑1 (MQ=255)
                                                                                                                      aaaTGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGc                         >  1:144318/1‑150 (MQ=255)
                                                                                                                      aaaTGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGc                         >  1:359892/1‑150 (MQ=255)
                                                                                                                       aaTGTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCAt                       >  1:124989/1‑151 (MQ=255)
                                                                                                                          gTTTTACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCAttg                     <  4:1530022/150‑1 (MQ=255)
                                                                                                                            tttACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCAttgtt                   <  3:214435/150‑1 (MQ=255)
                                                                                                                            tttACTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCAttgtt                   <  3:445454/150‑1 (MQ=255)
                                                                                                                               aCTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAgc               <  2:872046/151‑1 (MQ=255)
                                                                                                                               aCTGGCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAgc               >  3:221747/1‑151 (MQ=255)
                                                                                                                                  ggCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGTCATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCt            <  4:373725/151‑1 (MQ=255)
                                                                                                                                   gCCGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTa           <  4:576569/151‑1 (MQ=255)
                                                                                                                                    ccGCACGCAGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTAt          >  2:1084719/1‑151 (MQ=255)
                                                                                                                                      gcacgcaGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTAttt        <  2:1258617/151‑1 (MQ=255)
                                                                                                                                       cacgcaGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTAtttt       >  2:1524509/1‑151 (MQ=255)
                                                                                                                                       cacgcaGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTAtttt       <  4:816464/151‑1 (MQ=255)
                                                                                                                                         cgcaGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTAc     <  4:1356999/151‑1 (MQ=255)
                                                                                                                                          gcaGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACg    >  4:1543767/1‑151 (MQ=255)
                                                                                                                                           caGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCTCGCAATCCGTGGCATTGTTGAGCGCTATTTTACgt   <  2:457042/151‑1 (MQ=255)
                                                                                                                                            aGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACgtg  >  4:550325/1‑151 (MQ=255)
                                                                                                                                            aGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACgtg  <  1:330190/151‑1 (MQ=255)
                                                                                                                                            aGGGCGCCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACgtg  >  2:281658/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TCAGCTCTTCTGCCCCAACGCCGCCGCTCAGAGCGCGCCAGGTGTTGTTACCAAAGGCAACCACCGCACCAAGATGCGCGTCCGGGAATTTCGCTTCAAAAGTTGCCAGTTTGTCGGCAAATGTTTTACTGGCCGCACGCAGGGCGTCAACTTCCCCTTTCACGTTGGCTTCGATCCAAATCGCCGCGCGGCAATGTTCTGGCAAAATGCCACTCTGAACCTGAGACATTGTTCCTCCTGAAAATAATAATGCCACGCAATCCGTGGCATTGTTGAGCGCTATTTTACGTG  >  NC_000913/2550317‑2550607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: