Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,596,461 T→C T93A (ACC→GCC)  ypfJ ← putative neutral zinc metallopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,596,4610TC100.0% 409.5 / NA 110T93A (ACC→GCC) ypfJputative neutral zinc metallopeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (52/58);  total (52/58)

Only 100 of 110 total aligned reads displayed.

ATAAACCGTGCCATCCGCCGGGCAATAGAACGGCCCCATTATGGACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCAC  >  NC_000913/2596318‑2596608
                                                                                                                                               |                                                                                                                                                   
ctaAACCGTGCCATCCGCCGGGCAATAGAACGGCCCCATTCTGGACTGCCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGGACTGTTGTCCCCAGGCGTCTTc                                                                                                                                               >  1:92045/2‑150 (MQ=255)
     ccGTGCCATCCGCCGGGCAATAGAACGGCCCCATTATGGACTGGCCCGCCCCGCAGCCGGTCCGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTTCCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGt                                                                                                                                         <  2:1094053/151‑1 (MQ=255)
            aTCCGCCGGGCAATAGAACGGCCCCATTATGGACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGa                                                                                                                                  <  4:1567989/151‑1 (MQ=255)
              ccgccgGGCAATAGAACGGCCCCATTATGGACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGaa                                                                                                                                 <  4:1362343/150‑1 (MQ=255)
              ccgccgGGCAATAGAACGGCCCCATTATGGACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAAt                                                                                                                                >  2:1227120/1‑151 (MQ=255)
              ccgccgGGCAATAGAACGGCCCCATTATGGACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAAt                                                                                                                                <  2:1261725/151‑1 (MQ=255)
              ccgccgGGCAATAGAACGGCCCCATTATGGACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAAt                                                                                                                                <  2:673993/151‑1 (MQ=255)
                  cgGGCAATAGAACGGCCCCATTATGGACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCAcc                                                                                                                            >  4:1550377/1‑151 (MQ=255)
                           gAACGGCCCCATTATGGACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAAt                                                                                                                   <  4:118978/151‑1 (MQ=255)
                              cGGCCCCATTATGGACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAAtttt                                                                                                                <  1:289574/151‑1 (MQ=255)
                               ggCCCCATTATGGACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTg                                                                                                               <  4:435363/151‑1 (MQ=255)
                                gCCCCATTATGGACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGc                                                                                                              <  2:1556376/151‑1 (MQ=255)
                                gCCCCATTATGGACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGc                                                                                                              <  1:27248/151‑1 (MQ=255)
                                 ccccATTATGGACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCg                                                                                                             >  2:1543201/1‑151 (MQ=255)
                                    cATTATGGACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCt                                                                                                          >  3:283228/1‑151 (MQ=255)
                                    cATTATGGACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCt                                                                                                          <  3:177520/151‑1 (MQ=255)
                                       tATGGACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCg                                                                                                       >  4:1261283/1‑151 (MQ=255)
                                          ggACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCt                                                                                                    >  2:435515/1‑151 (MQ=255)
                                           gACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCtt                                                                                                   >  4:1678248/1‑151 (MQ=255)
                                            aCTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTc                                                                                                  <  4:1227154/151‑1 (MQ=255)
                                               ggCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCa                                                                                                 <  2:1282459/149‑1 (MQ=255)
                                               ggCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCAtt                                                                                               <  2:1029639/151‑1 (MQ=255)
                                               ggCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCAt                                                                                                <  2:1340489/150‑1 (MQ=255)
                                                gcccgcccCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCAttt                                                                                              <  2:1157681/151‑1 (MQ=255)
                                                  ccgccccgcAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTgg                                                                                            >  2:750577/1‑151 (MQ=255)
                                                   cgccccgcAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTggg                                                                                           >  2:1552943/1‑151 (MQ=255)
                                                    gccccgcAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGc                                                                                          >  2:1000481/1‑151 (MQ=255)
                                                         gcAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAAtt                                                                                     <  2:1365684/151‑1 (MQ=255)
                                                          cAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTg                                                                                    >  1:214284/1‑151 (MQ=255)
                                                             ccGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGAcc                                                                                 <  3:133171/151‑1 (MQ=255)
                                                             ccGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGAcc                                                                                 <  2:1571729/151‑1 (MQ=255)
                                                              cGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGCGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGAcc                                                                                 >  2:1597338/1‑150 (MQ=255)
                                                               ggTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACcgc                                                                               >  3:136599/1‑151 (MQ=255)
                                                                gTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACcgcg                                                                              >  4:1002838/1‑151 (MQ=255)
                                                                  aCGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGtt                                                                            >  4:779419/1‑151 (MQ=255)
                                                                  aCGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGtt                                                                            <  2:120539/151‑1 (MQ=255)
                                                                  aCGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGtt                                                                            >  2:166419/1‑151 (MQ=255)
                                                                   cgcgTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTg                                                                           >  4:769044/1‑151 (MQ=255)
                                                                    gcgTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGa                                                                          <  1:334463/151‑1 (MQ=255)
                                                                     cgTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAt                                                                         >  3:327444/1‑151 (MQ=255)
                                                                     cgTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAt                                                                         >  2:868808/1‑151 (MQ=255)
                                                                     cgTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGCCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAt                                                                         <  2:263310/151‑1 (MQ=255)
                                                                       acATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAttg                                                                       >  2:19065/2‑151 (MQ=255)
                                                                         aTTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAttgtt                                                                     <  4:884058/151‑1 (MQ=255)
                                                                          ttCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGAGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAttgtt                                                                     >  4:324112/1‑150 (MQ=255)
                                                                           tCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAttgttgt                                                                   >  2:556941/1‑151 (MQ=255)
                                                                           tCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAttgttg                                                                    <  4:313569/150‑1 (MQ=255)
                                                                           tCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAttgttg                                                                    <  2:1061199/150‑1 (MQ=255)
                                                                             cACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAttgttgttg                                                                 <  2:435275/151‑1 (MQ=255)
                                                                             cACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGAttgttgttg                                                                 <  4:885021/151‑1 (MQ=255)
                                                                               cGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTggg                                                               <  1:217532/151‑1 (MQ=255)
                                                                                 gTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGaa                                                             <  2:833006/151‑1 (MQ=255)
                                                                                   aCATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAAc                                                           <  4:603792/151‑1 (MQ=255)
                                                                                   aCATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAAc                                                           <  2:1008857/151‑1 (MQ=255)
                                                                                   aCATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAAc                                                           >  2:1408575/1‑151 (MQ=255)
                                                                                     aTGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAAcca                                                         <  2:271773/151‑2 (MQ=255)
                                                                                     aTGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCg                                                         <  4:184325/151‑1 (MQ=255)
                                                                                       gACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCgg                                                        <  4:743047/150‑1 (MQ=255)
                                                                                       gACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGc                                                       <  4:910619/151‑1 (MQ=255)
                                                                                         ccAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTg                                                     >  2:79539/1‑151 (MQ=255)
                                                                                         ccAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTg                                                     <  4:102586/151‑1 (MQ=255)
                                                                                         ccAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTg                                                     >  2:305488/1‑151 (MQ=255)
                                                                                         ccAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTg                                                     <  2:812582/151‑1 (MQ=255)
                                                                                          cAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGc                                                    >  2:1119920/1‑151 (MQ=255)
                                                                                           aGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGcc                                                   >  2:48246/1‑151 (MQ=255)
                                                                                            gCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGccc                                                  >  3:100641/1‑151 (MQ=255)
                                                                                               tCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCgg                                                >  2:8226/1‑150 (MQ=255)
                                                                                                 ggTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGtca                                             <  3:296467/151‑1 (MQ=255)
                                                                                                  gTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGtcat                                            <  3:29281/151‑1 (MQ=255)
                                                                                                  gTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGtcat                                            >  2:1675823/1‑151 (MQ=255)
                                                                                                    tGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGtcatc                                           >  2:917235/1‑150 (MQ=255)
                                                                                                    tGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGtcatc                                           >  1:63660/1‑150 (MQ=255)
                                                                                                     gCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCaa                                         <  4:1396301/151‑1 (MQ=255)
                                                                                                        gATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAAcc                                       <  4:865922/150‑1 (MQ=255)
                                                                                                         aTAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCg                                     >  1:186627/1‑151 (MQ=255)
                                                                                                           aGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGt                                   <  2:1358889/151‑1 (MQ=255)
                                                                                                             gTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTa                                 <  2:432727/151‑1 (MQ=255)
                                                                                                                 tACCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATc                             >  2:378567/1‑151 (MQ=255)
                                                                                                                  aCCCATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCa                            <  2:1058445/151‑1 (MQ=255)
                                                                                                                   cccATCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCaa                           <  1:296418/151‑1 (MQ=255)
                                                                                                                      aTCTTCTCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAacac                        >  2:1269076/1‑151 (MQ=255)
                                                                                                                       tcttctCGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACAcc                       >  4:1294438/1‑151 (MQ=255)
                                                                                                                           ctcGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCAt                     >  1:403322/1‑149 (MQ=255)
                                                                                                                           ctcGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCAt                     >  4:840030/1‑149 (MQ=255)
                                                                                                                            tcGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCAtagt                  >  4:361565/1‑151 (MQ=255)
                                                                                                                             cGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCAtagt                  >  4:757610/1‑150 (MQ=255)
                                                                                                                             cGAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCAtagt                  <  1:327427/150‑1 (MQ=255)
                                                                                                                              gAACTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCAtagt                  >  2:804642/1‑149 (MQ=255)
                                                                                                                               aaCTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGc               >  4:107903/1‑151 (MQ=255)
                                                                                                                                 cTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCt             >  2:350032/1‑151 (MQ=255)
                                                                                                                                 cTGTTGTCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCt             >  2:1307320/1‑151 (MQ=255)
                                                                                                                                   gttgtCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGcca             <  2:178475/149‑2 (MQ=255)
                                                                                                                                   gttgtCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCt             <  1:307983/149‑1 (MQ=255)
                                                                                                                                      gtCCCCAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAAc        >  2:329434/1‑151 (MQ=255)
                                                                                                                                         cccAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAg     <  2:588961/151‑1 (MQ=255)
                                                                                                                                          ccAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGc    >  4:297160/1‑151 (MQ=255)
                                                                                                                                           cAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGc    >  4:1133435/1‑150 (MQ=255)
                                                                                                                                           cAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCAGCCCGGTCATCAACCCGGTTAAATCAACCCCATAGTAGCCTGCAACCAGCa   <  2:888419/151‑1 (MQ=255)
                                                                                                                                           cAGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCAGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCa   <  3:86501/151‑1 (MQ=255)
                                                                                                                                            aGGCGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCAc  <  2:149560/151‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
ATAAACCGTGCCATCCGCCGGGCAATAGAACGGCCCCATTATGGACTGGCCCGCCCCGCAGCCGGTACGCGTCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCAC  >  NC_000913/2596318‑2596608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: