Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,700,875 T→C T42A (ACG→GCG)  acpS ← holo‑[acyl‑carrier‑protein] synthase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,700,8750TC98.0% 367.5 / ‑4.2 100T42A (ACG→GCG) acpSholo‑[acyl‑carrier‑protein] synthase 1
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (48/50);  total (49/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 102 total aligned reads displayed.

AGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCACGCGCTTCCAGCATC  >  NC_000913/2700727‑2701019
                                                                                                                                                    |                                                                                                                                                
agccgtagccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGctt                                                                                                                                                >  2:599259/1‑151 (MQ=255)
  ccgtagccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTc                                                                                                                                              >  3:259442/1‑151 (MQ=255)
  ccgtagccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTc                                                                                                                                              <  1:425912/151‑1 (MQ=255)
       gccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGAt                                                                                                                                         <  1:449445/151‑1 (MQ=255)
       gccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGAt                                                                                                                                         <  2:1327682/151‑1 (MQ=255)
        ccgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATa                                                                                                                                        <  3:182869/151‑1 (MQ=255)
         cgtGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGAt                                                                                                                                         >  4:1523447/1‑149 (MQ=255)
             gTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGccc                                                                                                                                    >  4:440498/1‑150 (MQ=255)
             gTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCa                                                                                                                                   <  2:339707/151‑1 (MQ=255)
              tttGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCAt                                                                                                                                  >  4:1472561/1‑151 (MQ=255)
                 gCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTc                                                                                                                                <  4:1684419/150‑1 (MQ=255)
                 gCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCg                                                                                                                               <  2:802843/151‑1 (MQ=255)
                     aGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTAt                                                                                                                           <  2:741638/151‑1 (MQ=255)
                      gCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATc                                                                                                                          >  2:943329/1‑151 (MQ=255)
                       cTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCg                                                                                                                         >  4:46514/1‑151 (MQ=255)
                          atcatTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCtt                                                                                                                      >  4:1489161/1‑151 (MQ=255)
                             atTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAAt                                                                                                                   >  4:1492652/1‑151 (MQ=255)
                             atTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAAt                                                                                                                   >  2:69003/1‑151 (MQ=255)
                               tGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATAc                                                                                                                 >  2:910678/1‑151 (MQ=255)
                                 taTACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATAcg                                                                                                                >  2:1657002/2‑150 (MQ=255)
                                 aaTACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATAcgc                                                                                                               <  2:257964/151‑1 (MQ=255)
                                  aTACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATAcgcg                                                                                                              >  2:564163/1‑151 (MQ=255)
                                    aCTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACgcggc                                                                                                            <  4:933348/151‑1 (MQ=255)
                                        cAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGc                                                                                                        <  4:1594143/151‑1 (MQ=255)
                                        cAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACACTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTTCGCTTTCCAGATAGCCCATTCGTTATCTCTTAATACGCGTCGTg                                                                                                         >  4:1570755/1‑150 (MQ=255)
                                          aaTTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGcc                                                                                                       >  4:1534729/1‑150 (MQ=255)
                                          aaTTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGcc                                                                                                       >  2:635693/1‑150 (MQ=255)
                                          aaTTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCa                                                                                                      <  4:720392/151‑1 (MQ=255)
                                          aaTTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCa                                                                                                      <  2:263204/151‑1 (MQ=255)
                                          aaTTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGGGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGgcgcgc                                                                                                        >  2:866102/1‑149 (MQ=255)
                                             tGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAgg                                                                                                    <  2:722282/150‑1 (MQ=255)
                                              gATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCg                                                                                                  >  4:321261/1‑151 (MQ=255)
                                               aTTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCg                                                                                                  >  4:281088/1‑150 (MQ=255)
                                               aTTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCg                                                                                                  >  4:1122837/1‑150 (MQ=255)
                                                  aaaCGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATc                                                                                               >  2:1640508/1‑150 (MQ=255)
                                                   aaCGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCAc                                                                                             <  4:1001511/151‑1 (MQ=255)
                                                     cGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCg                                                                                           >  4:535978/1‑151 (MQ=255)
                                                      gCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCgg                                                                                          >  4:1491111/1‑151 (MQ=255)
                                                      gCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCgg                                                                                          >  4:1594385/1‑151 (MQ=255)
                                                         aGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATc                                                                                       >  2:768525/1‑151 (MQ=255)
                                                               ttGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCg                                                                                  >  2:210058/1‑150 (MQ=255)
                                                                     aTCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCg                                                                           >  4:646803/1‑151 (MQ=255)
                                                                      tCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGc                                                                          >  4:1298975/1‑151 (MQ=255)
                                                                      tCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGc                                                                          >  1:437848/1‑151 (MQ=255)
                                                                      tCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGc                                                                          >  2:384425/1‑151 (MQ=255)
                                                                        ccGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCtt                                                                        >  2:583848/1‑151 (MQ=255)
                                                                         cGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTc                                                                       <  4:1512571/151‑1 (MQ=255)
                                                                          ggTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCg                                                                      >  4:679443/1‑151 (MQ=255)
                                                                             gCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCTGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATGCGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGAt                                                                    <  2:1673508/150‑1 (MQ=255)
                                                                                 aaCGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgcgag                                                               <  4:440714/151‑1 (MQ=255)
                                                                                 aaCGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgcgag                                                               <  4:911854/151‑1 (MQ=255)
                                                                                  aCGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgcgagc                                                              <  3:150995/151‑1 (MQ=255)
                                                                                  aCGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgcgagc                                                              <  2:1301770/151‑1 (MQ=255)
                                                                                  aCGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgcgagc                                                              <  2:1425696/151‑1 (MQ=255)
                                                                                  aCGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgcgagc                                                              <  2:1067126/151‑1 (MQ=255)
                                                                                   cGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgcgagcg                                                             <  2:1649859/151‑1 (MQ=255)
                                                                                     ctgttGCTGCGGCTTCTTTCACAGCGAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGCGCGCTTTCCAGATAGCCCAGGCGTTATCGATTAATACTAGGCGTGCCATTCGATCACCGGTTCGGCCGATCACCGCTTCGATgcgagcgtt                                                           >  1:360452/4‑149 (MQ=255)
                                                                                      ttttGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGAtc                                                          >  3:424216/1‑151 (MQ=255)
                                                                                         tgctgcGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTcc                                                       >  4:1023023/1‑151 (MQ=255)
                                                                                         tgctgcGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTcc                                                       >  4:888187/1‑151 (MQ=255)
                                                                                                gCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAatat                                                >  1:221515/1‑151 (MQ=255)
                                                                                                   tcttTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATct                                              <  2:1679915/150‑2 (MQ=255)
                                                                                                   tcttTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCg                                             >  1:7876/1‑151 (MQ=255)
                                                                                                      ttCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGt                                            <  4:555961/149‑1 (MQ=255)
                                                                                                           aGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTaaa                                     <  4:770824/151‑1 (MQ=255)
                                                                                                           aGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTaa                                      >  4:671440/1‑150 (MQ=255)
                                                                                                               aaaCGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTa                                 <  4:1636880/151‑1 (MQ=255)
                                                                                                                aaCGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTaa                                <  2:280642/151‑1 (MQ=255)
                                                                                                                       cGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGcc                         <  4:682288/151‑1 (MQ=255)
                                                                                                                       cGCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGcc                         >  2:1470303/1‑151 (MQ=255)
                                                                                                                        gCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGcc                         >  4:665474/1‑150 (MQ=255)
                                                                                                                        gCCAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCa                        <  4:216429/151‑1 (MQ=255)
                                                                                                                         ccAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCAt                       >  2:77058/1‑151 (MQ=255)
                                                                                                                          cAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCAtt                      <  4:498590/151‑1 (MQ=255)
                                                                                                                          cAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCAtt                      >  4:1523484/1‑151 (MQ=255)
                                                                                                                          cAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCAtt                      <  1:305900/151‑1 (MQ=255)
                                                                                                                          cAGAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATAGCCGTGCCTAAACCTAATATTGCCAtt                      <  2:543946/151‑1 (MQ=255)
                                                                                                                            gAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCt                                        >  3:44789/1‑131 (MQ=255)
                                                                                                                            gAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCt                                        <  1:44786/131‑1 (MQ=255)
                                                                                                                            gAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAg                    <  4:1331768/151‑1 (MQ=255)
                                                                                                                            gAAAACGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAg                    <  2:1096117/151‑1 (MQ=255)
                                                                                                                             aaaaCGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGc                   >  1:190963/1‑151 (MQ=255)
                                                                                                                             aaaaCGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGc                   <  4:564939/151‑1 (MQ=255)
                                                                                                                               aaCGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCa                 <  4:1476674/151‑1 (MQ=255)
                                                                                                                               aaCGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCa                 <  4:1145561/151‑1 (MQ=255)
                                                                                                                                aCGCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCAc                >  2:53528/1‑151 (MQ=255)
                                                                                                                                  gCACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCAcgc              <  4:384986/151‑1 (MQ=255)
                                                                                                                                   cACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCAcgcg             <  4:530827/151‑1 (MQ=255)
                                                                                                                                   cACCGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCAcgcg             >  2:1424642/1‑151 (MQ=255)
                                                                                                                                      cGGCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCACGCGCtt          <  3:436487/151‑1 (MQ=255)
                                                                                                                                       ggCGGGTGTTGCGCTTTCCGGATAGCCCATTCGTTATCGCTTAATCCGCGGCGTGCCGGGCGATCACCGGTTCGGGGGATCACGGCTTCGATGCGGGCGATCTCCACAATATCCGTGCCTAAACCTATTTTTCCCATAGCCACGCGCTTcc        <  2:1150761/151‑1 (MQ=255)
                                                                                                                                        gCTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCACGCGCTTcc        <  3:183835/151‑1 (MQ=255)
                                                                                                                                         cTGGTGGTGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCACGCGCTTcc        >  1:180048/1‑150 (MQ=255)
                                                                                                                                           ggtggtGCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCACGCGCTTCCa       <  2:44912/149‑1 (MQ=255)
                                                                                                                                            gtggtgCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCCTGCCAGGCGATCATCGTATTTTTTTATTTCCGATTCGAGGCGAGCCACCTCCACAATATCAGTGCCTACCCCTTTTACCGCCAATAGCCCCGCGCCTccc       <  2:1683256/148‑2 (MQ=255)
                                                                                                                                            gtggtgCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCACGCGCTTCCAGc     >  3:256786/1‑150 (MQ=255)
                                                                                                                                            gtggtgCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCACGCGCTTCCAGCa    <  4:1642512/151‑1 (MQ=255)
                                                                                                                                            gtggtgCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCACGCGCTTCCAGCa    <  2:1521582/151‑1 (MQ=255)
                                                                                                                                            gtggtgCGCTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCACGCGCTTCCAGCa    <  4:944552/151‑1 (MQ=255)
                                                                                                                                                 gcgcTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCACGCGCTTCCAGCATc  >  3:300035/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATTGCCATTAGCCACGCGCTTCCAGCATC  >  NC_000913/2700727‑2701019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: