Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,711,683 T→C W422R (TGG→CGG)  nadB → quinolinate synthase, L‑aspartate oxidase (B protein) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,711,6830TC100.0% 377.7 / NA 103W422R (TGG→CGG) nadBquinolinate synthase, L‑aspartate oxidase (B protein) subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/55);  total (48/55)

Only 100 of 104 total aligned reads displayed.

ATTGGCGAGGTGAGTTATACCGGCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATGCTC  >  NC_000913/2711536‑2711829
                                                                                                                                                   |                                                                                                                                                  
aTTGGCGAGGTGAGTTATACCGGCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCggg                                                                                                                                                 <  4:1650568/151‑1 (MQ=255)
  tGGCGAGGTGAGTTATACCGGCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGa                                                                                                                                                <  3:27562/150‑1 (MQ=255)
   ggCGAGGTGAGTTATGCCGGCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGAt                                                                                                                                               <  4:1560862/150‑1 (MQ=255)
      gAGGTGAGTTATACCGGCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGaa                                                                                                                                            >  1:236457/1‑150 (MQ=255)
         gTGAGTTATACCGGCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGcc                                                                                                                                        <  2:1403779/151‑1 (MQ=255)
         gTGAGTTATACCGGCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGcc                                                                                                                                        >  4:1398672/1‑151 (MQ=255)
         gTGAGTTATACCGGCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGcc                                                                                                                                        >  4:1203506/1‑151 (MQ=255)
           gAGTTATACCGGCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCcg                                                                                                                                       <  4:204807/150‑1 (MQ=255)
           gAGTTATACCGGCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCcg                                                                                                                                       <  4:322268/150‑1 (MQ=255)
             gTTATACCGGCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGt                                                                                                                                    <  2:1087778/151‑1 (MQ=255)
              ttATACCGGCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGtt                                                                                                                                   >  4:885063/1‑151 (MQ=255)
               tataCCGGCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGtt                                                                                                                                   <  2:1216435/150‑1 (MQ=255)
                ataCCGGCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTg                                                                                                                                  >  2:1297021/1‑150 (MQ=255)
                   ccGGCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTgaga                                                                                                                               >  4:1375466/1‑150 (MQ=255)
                   ccGGCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTgaga                                                                                                                               <  1:382212/150‑1 (MQ=255)
                   ccGGCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTgaga                                                                                                                               <  2:1232891/150‑1 (MQ=255)
                    cGGCTTGCCCGGCGCTAACCGCATGGCATCGAATTCATTGCTGGAGTGTCTGGTCGATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGAGGAAAGCCGCGTTgaga                                                                                                                               <  2:649390/149‑1 (MQ=255)
                     ggCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAAcc                                                                                                                            >  4:396428/1‑151 (MQ=255)
                     ggCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAAcc                                                                                                                            >  1:432402/1‑151 (MQ=255)
                       cTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCt                                                                                                                          >  4:1232528/1‑151 (MQ=255)
                        ttACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTg                                                                                                                         >  3:56717/1‑151 (MQ=255)
                         tACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTg                                                                                                                         <  1:56713/150‑1 (MQ=255)
                         tACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTg                                                                                                                         <  3:64629/150‑1 (MQ=255)
                          acacGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGAc                                                                                                                       >  2:461836/1‑151 (MQ=255)
                          acacGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGAc                                                                                                                       >  2:1454141/1‑151 (MQ=255)
                             cGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGaa                                                                                                                    <  4:1586647/151‑1 (MQ=255)
                             cGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGa                                                                                                                     <  2:226155/150‑1 (MQ=255)
                               gcgcTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACg                                                                                                                  >  4:1359764/1‑151 (MQ=255)
                                cgcTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACgg                                                                                                                 >  4:812075/1‑151 (MQ=255)
                                cgcTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACgg                                                                                                                 <  2:1302111/151‑1 (MQ=255)
                                 gcTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACggg                                                                                                                >  3:444827/1‑151 (MQ=255)
                                  cTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACggg                                                                                                                >  4:1008601/1‑150 (MQ=255)
                                   tAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCAGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGgt                                                                                                               >  2:1601597/1‑150 (MQ=255)
                                    aaCCGCATGGCCTTGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATTTCACCAGACGTATGCCTTATGCCCACGACATCAGTCCGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGgt                                                                                                               <  2:859891/149‑1 (MQ=255)
                                    aaCCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGgtag                                                                                                             <  4:390431/151‑1 (MQ=255)
                                    aaCCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGgt                                                                                                               >  2:384176/1‑149 (MQ=255)
                                    aaCCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGgt                                                                                                               >  4:44648/1‑149 (MQ=255)
                                      ccGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGgtagta                                                                                                           <  1:150375/151‑1 (MQ=255)
                                         cATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAAtt                                                                                                        <  1:118295/151‑1 (MQ=255)
                                          aTGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAGCCCTGACGAACGGGTAGTAATTc                                                                                                       <  3:394988/151‑1 (MQ=255)
                                          aTGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTc                                                                                                       <  2:1294921/151‑1 (MQ=255)
                                          aTGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTc                                                                                                       <  4:315006/151‑1 (MQ=255)
                                              ccTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCa                                                                                                   <  3:445843/151‑1 (MQ=255)
                                               cTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCAt                                                                                                  >  4:1580737/1‑151 (MQ=255)
                                               cTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCAt                                                                                                  >  4:666450/1‑151 (MQ=255)
                                                  gAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAAc                                                                                               >  2:689420/1‑151 (MQ=255)
                                                       cATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGc                                                                                           >  2:590009/1‑150 (MQ=255)
                                                       cATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCa                                                                                          <  4:1256003/151‑1 (MQ=255)
                                                        aTTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCAc                                                                                         >  4:1585093/1‑151 (MQ=255)
                                                        aTTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCAc                                                                                         <  4:1659675/151‑1 (MQ=255)
                                                           gCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACg                                                                                        >  3:128787/1‑149 (MQ=255)
                                                             tGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCt                                                                                    >  2:1353446/1‑151 (MQ=255)
                                                              ggAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGc                                                                                     <  4:439350/149‑1 (MQ=255)
                                                              ggAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGc                                                                                     <  2:281561/149‑1 (MQ=255)
                                                              ggAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTa                                                                                   <  2:860611/151‑1 (MQ=255)
                                                              ggAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTCATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCAGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGc                                                                                     <  4:803253/149‑1 (MQ=255)
                                                                aGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACg                                                                                 <  3:194025/151‑1 (MQ=255)
                                                                    tCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTg                                                                             >  4:548935/1‑151 (MQ=255)
                                                                    tCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTg                                                                             >  3:427781/1‑151 (MQ=255)
                                                                      tGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGGATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGtt                                                                           >  4:1294585/1‑151 (MQ=255)
                                                                            aTGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTAtgtg                                                                     >  4:1013714/1‑151 (MQ=255)
                                                                            aTGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTAtgtg                                                                     >  4:1566051/1‑151 (MQ=255)
                                                                               gCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGa                                                                  <  4:496082/151‑1 (MQ=255)
                                                                               gCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGa                                                                  <  2:583557/151‑1 (MQ=255)
                                                                                cTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGAt                                                                 >  2:1224511/1‑151 (MQ=255)
                                                                                  ggTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGAt                                                                 <  2:1506832/149‑1 (MQ=255)
                                                                                     cggcggcggAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACg                                                             <  1:444808/150‑1 (MQ=255)
                                                                                      ggcggcggAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTCCCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGtt                                                           <  2:1626282/151‑1 (MQ=255)
                                                                                          gcggAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGc                                                        >  2:828627/1‑150 (MQ=255)
                                                                                           cggAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCAt                                                      >  3:137417/1‑151 (MQ=255)
                                                                                            ggAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCAtt                                                     >  4:836523/1‑151 (MQ=255)
                                                                                             gAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATtg                                                    <  2:1419011/151‑1 (MQ=255)
                                                                                               aGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATtgtg                                                  >  2:1056759/1‑151 (MQ=255)
                                                                                               aGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATtgtg                                                  <  4:48029/151‑1 (MQ=255)
                                                                                                gATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTgc                                                 <  4:403900/151‑1 (MQ=255)
                                                                                                 atatCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTgcg                                                <  4:1298653/151‑1 (MQ=255)
                                                                                                  tatCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTgcgc                                               >  4:985825/1‑151 (MQ=255)
                                                                                                     cACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGcaca                                            <  2:437649/151‑1 (MQ=255)
                                                                                                      aCCAGACGTAGGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGCTTATGTGGGATTACGTTCGCCTTGTgcgcgaaa                                           <  2:382026/151‑5 (MQ=255)
                                                                                                        cAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACg                                         <  4:492813/151‑1 (MQ=255)
                                                                                                         aGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGa                                        <  2:1316580/151‑1 (MQ=255)
                                                                                                          gACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGaa                                       <  1:266172/151‑1 (MQ=255)
                                                                                                               aTGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGcc                                  <  4:663600/151‑1 (MQ=255)
                                                                                                                tGCCTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCt                                 >  4:1596383/1‑151 (MQ=255)
                                                                                                                  ccTTATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTgg                               <  2:38851/151‑1 (MQ=255)
                                                                                                                     tATGCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAAc                            >  2:812425/1‑151 (MQ=255)
                                                                                                                        gCCCACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAAcgcg                         <  2:179568/151‑1 (MQ=255)
                                                                                                                         cccACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACgcgc                        >  4:344041/1‑151 (MQ=255)
                                                                                                                          ccACGACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGcc                       >  1:88544/1‑151 (MQ=255)
                                                                                                                              gACATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTg                    >  2:801673/1‑150 (MQ=255)
                                                                                                                                cATCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTgcgg                 >  4:716714/1‑151 (MQ=255)
                                                                                                                                  tCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTgcggcg               >  2:653412/1‑151 (MQ=255)
                                                                                                                                  tCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTgcggcg               >  2:256875/1‑151 (MQ=255)
                                                                                                                                  tCAGTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTgcggcg               >  1:119234/1‑151 (MQ=255)
                                                                                                                                     gTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGAt            <  2:1203768/151‑1 (MQ=255)
                                                                                                                                     gTACGTTACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGAt            <  4:1624061/151‑1 (MQ=255)
                                                                                                                                           tACCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCa       <  4:1417471/150‑1 (MQ=255)
                                                                                                                                            aCCGCCGCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCAt      <  4:885518/150‑1 (MQ=255)
                                                                                                                                              cgccgcGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATGCt   <  3:75435/151‑1 (MQ=255)
                                                                                                                                               gccgCGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATGCTc  <  4:1128719/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ATTGGCGAGGTGAGTTATACCGGCTTACACGGCGCTAACCGCATGGCCTCGAATTCATTGCTGGAGTGTCTGGTCTATGGCTGGTCGGCGGCGGAAGATATCACCAGACGTATGCCTTATGCCCACGACATCAGTACGTTACCGCCGTGGGATGAAAGCCGCGTTGAGAACCCTGACGAACGGGTAGTAATTCAGCATAACTGGCACGAGCTACGTCTGTTTATGTGGGATTACGTTGGCATTGTGCGCACAACGAAGCGCCTGGAACGCGCCCTGCGGCGGATAACCATGCTC  >  NC_000913/2711536‑2711829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑