Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,478,3240TG42.5% 199.5 / 57.7 120G33G (GGT→GGG) ynbBputative CDP‑diglyceride synthase
Reads supporting (aligned to +/- strand):  ref base T (9/60);  new base G (51/0);  total (60/60)
Fisher's exact test for biased strand distribution p-value = 1.17e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

TGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACA  >  NC_000913/1478178‑1478470
                                                                                                                                                    |                                                                                                                                                    
tGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACTTGGGCG‑AAAAAGGGGGGAgg                                                                                                                                                  >  1:291795/1‑151 (MQ=255)
 gCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAgg                                                                                                                                                  <  2:307774/150‑1 (MQ=255)
 gCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAg                                                                                                                                                   <  4:301895/149‑1 (MQ=255)
 gCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGt                                                                                                                                                 <  4:67312/151‑1 (MQ=255)
  cGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTTCGGGGAG‑AAAAGGGGGGGAgg                                                                                                                                                  >  1:1119231/1‑149 (MQ=255)
        aTCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTa                                                                                                                                          <  3:1216283/151‑1 (MQ=255)
        aTCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAA‑GGGGTGAGGTTACATTAc                                                                                                                                         <  1:573028/151‑1 (MQ=255)
          cGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTATCCGAGAG‑AAAAAGGGGGGGGGGTACAtt                                                                                                                                           >  4:215054/1‑148 (MQ=255)
           gTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTCCCGGGAG‑AAAAGGGGGGGGGGGTACAtt                                                                                                                                           >  3:253500/1‑147 (MQ=255)
              gTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTAt                                                                                                                                     <  1:389053/150‑1 (MQ=255)
               tCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTACGGGGAG‑AAAAGGGGGGGAgggttaattttgtctt                                                                                                                                    >  1:1126453/1‑136 (MQ=255)
                cGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGGG‑AAAAGGGGGGGGGGGTTCATTAAGTAtt                                                                                                                                    >  3:871804/1‑149 (MQ=255)
                 ggAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑Gt                                                                                                                                <  3:1297385/151‑1 (MQ=255)
                     tgtgCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑GTAACGGGGGA‑AAAAGGGGGGGAGGTTAGATTATGTTTTT‑GTTcc                                                                                                                             >  3:1384390/1‑150 (MQ=255)
                      gtgCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCt‑g                                                                                                                          <  3:1043310/151‑1 (MQ=255)
                        gCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTACGGGGAG‑AAAAAGGGGGGGGGGTAAATTACGTATTC‑GTACCt                                                                                                                            >  2:425848/1‑148 (MQ=255)
                        gCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGAG‑AAAAGGGGGGGGGGGTACATTTCGTATTT‑GTATCttg                                                                                                                          >  1:1335921/1‑148 (MQ=255)
                              tATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTgggg                                                                                                                    <  1:1395120/149‑1 (MQ=255)
                              tATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTggg                                                                                                                     <  3:1240/148‑1 (MQ=255)
                              tATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAAGGGGGGAGGGTAAATTACGTATTC‑GTACCT‑Ggggggg                                                                                                                    >  3:1175542/1‑149 (MQ=255)
                               aTGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCAt                                                                                                                 <  1:1153882/151‑1 (MQ=255)
                               aTGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTAACGGAGAG‑AAAAGGGGGGGGGGGTAAATTACGTATTC‑GTACCT‑GGTGGGGGAt                                                                                                                 >  3:554341/1‑151 (MQ=255)
                                 gAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATc                                                                                                                <  4:475523/150‑1 (MQ=255)
                                 gAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGCTTACATTACGCATTC‑GTACCg‑ggtttcggatc                                                                                                                <  4:413872/150‑9 (MQ=255)
                                   gcgcTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGt                                                                                                              <  1:846052/150‑1 (MQ=255)
                                    cgcTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGAG‑GAAAGGGGGGGGGGGTACATTACCTATTT‑TTACCT‑GGGGGGGCgtcgtc                                                                                                             >  3:1295567/1‑150 (MQ=255)
                                     gcTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTACGGGGAG‑AAAAAGGGGGGAGGGTATAATTCGTATTC‑GTAACT‑GGTGGGGGATCGTCa                                                                                                            >  2:170444/1‑150 (MQ=255)
                                      cTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTAACGGGGAG‑GAAAGGGGGGGAGGGTACATTTCCTATTCTGTTCCT‑GGGGGGGctcgatctt                                                                                                           >  4:262240/1‑143 (MQ=255)
                                       tGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGAG‑AAAAGGGGGGGGGGGTAAATTATGTATTT‑GTACCT‑GGTGGGGGATCGTCAttt                                                                                                         >  4:222855/1‑151 (MQ=255)
                                        gAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTCCGGAGAG‑AAAAGGGGGGGAGGGTAAATTAAGTATTT‑CTCCCT‑GGTGGGGGATCGTCAttt                                                                                                         >  2:256695/1‑150 (MQ=255)
                                        gAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCAttt                                                                                                         <  3:954314/150‑1 (MQ=255)
                                        gAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCAttt                                                                                                         <  4:505753/150‑1 (MQ=255)
                                          aaTATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGtt                                                                                                      <  1:813511/151‑1 (MQ=255)
                                            tataATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTCTTGGGGG‑AAAAAGGGGGGAGAGTTCCTTTTGATTTT‑GTTCCT‑TGTGGGGGAGCGTCAtttttttt                                                                                                    >  1:692399/1‑151 (MQ=255)
                                             ataATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGtttt                                                                                                    <  3:67742/150‑1 (MQ=255)
                                              taATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGttttt                                                                                                   <  3:923082/150‑1 (MQ=255)
                                               aaTGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTAACGGAGAG‑GAAAGGGGGGGAGGGTACAATTAGGATTC‑GTACCT‑TGGGGGGGATCGTCATTTGtttttt                                                                                                  >  4:422403/1‑149 (MQ=255)
                                                   cTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTCCGGAGAG‑AAAAGGGGGGGAGGGTAAAATACGTATTC‑GTAACT‑GGTGGGGGATCGTCCTtttttttt                                                                                                   >  3:1233011/1‑145 (MQ=255)
                                                    tGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAAGGGGGGGGGTTAGATTAAGTATTC‑TTACCTGGGTGGGGC‑GCGGCATttttttttttattg                                                                                             >  1:1318791/1‑144 (MQ=255)
                                                    tGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTAACGGAGGG‑GAAAGGGGGGGGGGGTACATTACGTATTC‑GTTCCT‑GGTGGGGGATCGTCATttttttttttat                                                                                               >  4:499127/1‑144 (MQ=255)
                                                     ggAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTg                                                                                           <  3:619345/151‑1 (MQ=255)
                                                        aaaaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTCCGGAGAG‑AAAAAGGGGGGAGGGTAAATTACGTATTT‑GTAACT‑GGTGGGGGGTCGGCATTTTTTTTTTAATGGGGAtt                                                                                        >  1:350647/1‑151 (MQ=255)
                                                           aTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTCCGGGGGG‑AAAAAGGGGGGAGGGTTAATTACGTATTC‑TTACCT‑GGTGGGGGGTCGGCATTTGttttttttagggtatt                                                                                        >  1:1209754/1‑137 (MQ=255)
                                                           aTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTAACGGGGGG‑AAAAGGGGGGGGGGGTACATTACGTATTC‑GTACCT‑GGGGGGGGATCGTCATTTGTTTTTTAATGGTGAttt                                                                                       >  3:291424/1‑149 (MQ=255)
                                                            tctcTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTAACGGGGAG‑AAAAGGGGGGGAGGGTACATTACGTATTT‑GTACCT‑TGGGGGGGATCGTCATTTTTTTTTTTATGGGGAttt                                                                                       >  1:85323/1‑148 (MQ=255)
                                                             ctctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTATAGGGGAG‑AAAAAGGGGGGGGGGTTCATTTCGTATTT‑GTACCT‑TGTGGGGGATCGTGCTTTGTTTTTTTATTGGGAttt                                                                                       >  4:362962/1‑147 (MQ=255)
                                                             ctctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAAGGGGTGAGGGTACATTACGGATTC‑GTACCT‑GGGGGGGGATCGTCATTTGTTTTTCAATGGGGATTTTAgg                                                                                   >  2:76754/1‑151 (MQ=255)
                                                               ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCa                                                                                     <  3:659394/147‑1 (MQ=255)
                                                                 ggCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCa                                                                               <  1:1212048/151‑1 (MQ=255)
                                                                 ggCAACACTTATCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCa                                                                               <  3:721493/151‑1 (MQ=255)
                                                                  gCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTACCGG‑GGG‑GAAAGGGGGGGGGGGTACATTTCGGATTT‑CTACCT‑GGGGGGGGACCGCCCTTTTTTTTTTTATggggggtttagggt                                                                                 >  1:1137005/1‑136 (MQ=255)
                                                                    aaCACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑GAAAGGGGGGGGGGGTTCATTACCTATTC‑TTACCT‑GGTGGGGCATCGTCCTTTGTTTTTTAATGGTGATTTCAGGTCCAccc                                                                            >  2:545621/1‑149 (MQ=255)
                                                                    aaCACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGc                                                                            <  3:259021/151‑1 (MQ=255)
                                                                        cTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTgg                                                                         <  1:739915/150‑1 (MQ=255)
                                                                        cTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTgg                                                                         <  1:717668/150‑1 (MQ=255)
                                                                         ttttCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTAACCGGGGG‑AAAAGGGGGGGGGGGTAAATTAAGTATTC‑GTAACT‑GGGGGGGGGTCGTCCTTTGTTTTTTAATTGGGGTTTTAGGGGCACGCTgg                                                                         >  3:549681/1‑149 (MQ=255)
                                                                          tttCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGa                                                                        <  2:552673/149‑1 (MQ=255)
                                                                              gcgTTGTTAATTTTAGCAACGCTGATAGACCGTTTTCTGCGATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTGCATTACGTGTTC‑GTAGCT‑GGGGGGGGATCGTCATTTGTTTTTGAGTGGTGATTTGAGGTCGGCGCTGGAtgag                                                                    <  1:363615/149‑2 (MQ=255)
                                                                              gcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGAG‑AAAAGGGGGGGGGGGTACATTACGTATTC‑GTACCT‑GGTGGGGGATCGTCATTTTTTTTTTAATTGGGATTTTTGGTCCACGCTGGAtgacg                                                                   >  4:300921/1‑150 (MQ=255)
                                                                                     tAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGAG‑AAAAGGGGGGGGGGATACATTAAGTATTC‑GTAACT‑GGTGGGGCATCGTCATTTGTTTTTTAATGGGGATTTTAGGGCCACGCTGGAtgacgttgacgat                                                           >  2:12300/1‑149 (MQ=255)
                                                                                      aaTTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATgacgttgacgtt                                                           <  2:230150/150‑1 (MQ=255)
                                                                                      aaTTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGGGAGGGTACATTACGTATTC‑GTACCT‑GGGGGGGGATCGTCATTTTTTTTTTTATGGTGATTTTAGGGCCACGCTGGGTGACGTTGACGgtt                                                          >  2:112139/1‑151 (MQ=255)
                                                                                      aaTTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTAACGGGGAG‑GAAAGGGGGGGAGGGTACATTTATTTTTT‑GTACCT‑GGGGGGGGGTCGTCCTTTTTTTTTTTATTGTGGTTTTCAGTCCACGCTGGATGACGGTGACGttt                                                          >  4:367749/1‑151 (MQ=255)
                                                                                       aTTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTTACTGGGAGAAAAAGGGGGGGGGGTTA‑ATTAAGTATTT‑GTACCT‑TGGGGGGCATCGGCATTTTTTTTTTTAATGTGATTTTAGGTCCACGCTGGGTGGCGGTTGCGtttt                                                         >  4:441836/1‑151 (MQ=255)
                                                                                       aTTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGGG‑AAAAGGGGGGGGGGGTACATTACGTATTT‑GTACCT‑GGTGGGGGGTCGTCATTTTTTTTTTTATGGGGGTTTTTGGGCCACGCCGGGTGACGTTGGCGtttt                                                         >  2:284476/1‑151 (MQ=255)
                                                                                       aTTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGtttt                                                         <  1:1042468/151‑1 (MQ=255)
                                                                                         tttAGCTTCGCTGATAAACCGTTTTCTGCTATGGCGT‑TTACCGGAGAG‑AAAGGGGGGTGAGGTTACATTACATATTC‑GTACCT‑GGTGTGGCATCGTGATTTGTTTTTCAATGGTGATTTCAAGTCCACGCTGGATGACGTTGACGttttt                                                        <  4:190995/150‑1 (MQ=255)
                                                                                          ttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGGG‑AAAAGGGGGGGGGGGTTCATTACGTATTC‑GTACCT‑GGTGGGGGATCGGCATTTTTTTTTTTATGGTGGTTTTTGGGCCCCGCTGGGTGACGGTGGCGgttttt                                                       >  3:945368/1‑150 (MQ=255)
                                                                                              cAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGGGAG‑AAAAGGGGGGGAGGGTACATTACGTATTT‑GTACCT‑GGGGGGGGATCGTCATTTGTTTTTTTATGGTGGTTTTAGGGCCACGCTGGGTGACGTTGACGtttttt                                                       >  3:211486/1‑146 (MQ=255)
                                                                                              cAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCt                                                  <  1:193482/151‑1 (MQ=255)
                                                                                               aaCGCTGATAAACCGTTTTCTGCTATGGCGG‑TTAACGGCGAG‑AAAAAGGGGGGAGGGTACATTCAGAATTC‑GGACCT‑GGGGGGGGCTTCTCAATTGGTTTTTTATGGTGATTTTATGTCCCCGCTGGCAGACGTTTAAGTTTTTTggcc                                                   >  2:474698/1‑146 (MQ=255)
                                                                                                aCGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTCGCGCTGa                                                <  1:316437/151‑1 (MQ=255)
                                                                                                  tcTGATAAACCGTTTTCTGCTATGGCGG‑CTAACAGAGGG‑AAAAGGGGGGGGGGGTAAATTATGTGTTC‑GTAACT‑GGGGGGGCAGCGTCATTTGTTTTTTAATTGTGTTTTTAAGGCCCCGCCGGGGGAAGGTGGCGttttttttgct                                                  >  3:712215/2‑142 (MQ=255)
                                                                                                  gCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATa                                              <  3:291819/151‑1 (MQ=255)
                                                                                                     gATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGt                                           <  3:370997/151‑1 (MQ=255)
                                                                                                      aTAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGtt                                          <  1:685468/151‑1 (MQ=255)
                                                                                                        aaaCCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGttt                                         <  2:270826/150‑1 (MQ=255)
                                                                                                        aaaCCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGttt                                         <  1:915039/150‑1 (MQ=255)
                                                                                                        aaaCCGTTTTCTGCTATGGCGG‑TTACCCGGGAG‑AAAAAGGGGGGAGGGTACATTTCGTATTT‑GTACCT‑GGGGGGGGATCGGCCTTTGTTTTTTTATTGGGGTTTTTGGGCCACGCTGGGTGAGGTTGTCGGTTTTTTTGCTGGTAAGtttt                                        >  1:117800/1‑151 (MQ=255)
                                                                                                           ccGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTg                                     <  1:1047547/151‑1 (MQ=255)
                                                                                                                    gCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTg                               <  4:28242/148‑1 (MQ=255)
                                                                                                                     cTATGGCGG‑TTACCGGGGAG‑GAAAGGGGGGGGGGGTACATTACGTATTC‑GTACCT‑GGGGGGGGATCGTCATTTGTTTTTTTATGGGGGTTTTTGGTCCACGCCGGGTGGCGTTGACGTTTTTTTTGCTGGTAAGTTTTTTTGCAttttaa                            >  1:997911/1‑146 (MQ=255)
                                                                                                                        tGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGGGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAat                        >  3:499924/1‑151 (MQ=255)
                                                                                                                        tGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GGACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGaa                         >  1:594578/1‑150 (MQ=255)
                                                                                                                         ggCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAata                       <  1:795469/151‑1 (MQ=255)
                                                                                                                         ggCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTGTTTGCGCTGATAAGTTTTCTGGCCTTGAAAGaag                        <  4:106603/150‑2 (MQ=255)
                                                                                                                          gCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAatat                      >  3:664296/1‑151 (MQ=255)
                                                                                                                           cGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATAtt                     <  3:1246525/151‑1 (MQ=255)
                                                                                                                                tACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTa                  <  4:565445/150‑1 (MQ=255)
                                                                                                                                 aCCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACg                >  4:291178/1‑151 (MQ=255)
                                                                                                                                 aCCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACg                <  2:376043/151‑1 (MQ=255)
                                                                                                                                  ccGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGc               >  3:578606/1‑151 (MQ=255)
                                                                                                                                   cGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCt              >  2:565226/1‑151 (MQ=255)
                                                                                                                                     gagag‑aAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTta            <  3:1117710/151‑1 (MQ=255)
                                                                                                                                       gag‑aAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTtata          <  1:1163940/151‑1 (MQ=255)
                                                                                                                                       gag‑aAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTtat           >  1:24297/1‑150 (MQ=255)
                                                                                                                                            aaaGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTg      >  3:134034/1‑151 (MQ=255)
                                                                                                                                            aaaGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTg      <  4:204741/151‑1 (MQ=255)
                                                                                                                                               gggggTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTAc   <  3:953725/151‑1 (MQ=255)
                                                                                                                                                ggggTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACa  <  3:1054869/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGG‑TTACCGGAGAG‑AAAAGGGGGTGAGGTTACATTACGTATTC‑GTACCT‑GGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACA  >  NC_000913/1478178‑1478470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑