Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,048,7210AC39.2% 194.7 / 21.7 107T1262P (ACC→CCC) yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base A (48/17);  new base C (0/42);  total (48/59)
Fisher's exact test for biased strand distribution p-value = 3.14e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

TCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGG  >  NC_000913/2048572‑2048869
                                                                                                                                                     |                                                                                                                                                    
tcGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTa                                                                                                                                                      >  4:76938/1‑150 (MQ=255)
tcGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTAc                                                                                                                                                     >  1:57464/1‑151 (MQ=255)
tcGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTAc                                                                                                                                                     >  3:1336064/1‑151 (MQ=255)
 cGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTa                                                                                                                                                      <  3:527212/149‑1 (MQ=255)
  gCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTAccc                                                                                                                                                   >  4:44180/1‑151 (MQ=255)
   cAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTAcccc                                                                                                                                                  >  3:468678/1‑151 (MQ=255)
    aGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCg                                                                                                                                                 >  3:422944/1‑151 (MQ=255)
       cGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGc                                                                                                                                                >  2:435999/1‑149 (MQ=255)
          tGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGaa                                                                                                                                            <  3:424226/150‑1 (MQ=255)
          tGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGaa                                                                                                                                            <  3:12527/150‑1 (MQ=255)
          tGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGaa                                                                                                                                            <  2:263849/150‑1 (MQ=255)
           gCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAAc                                                                                                                                           <  2:381839/150‑1 (MQ=255)
             cTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGc                                                                                                                                        >  3:597245/1‑151 (MQ=255)
                tGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTcc                                                                                                                                     >  1:343755/1‑151 (MQ=255)
                aggTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTcc                                                                                                                                     >  1:1384223/2‑151 (MQ=255)
                 ggTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCg                                                                                                                                    <  2:287491/151‑1 (MQ=255)
                 ggTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCg                                                                                                                                    <  3:255812/151‑1 (MQ=255)
                  gTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCgg                                                                                                                                   >  3:1398610/1‑151 (MQ=255)
                      gaagaCGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAg                                                                                                                                >  3:237909/1‑150 (MQ=255)
                      gaagaCGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGc                                                                                                                               <  1:53592/151‑1 (MQ=255)
                       aagaCGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCg                                                                                                                              >  1:682302/1‑151 (MQ=255)
                         gaCGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGtc                                                                                                                            <  3:1274369/151‑1 (MQ=255)
                              ttACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCAc                                                                                                                       >  1:1125892/1‑151 (MQ=255)
                              ttACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCAc                                                                                                                       >  1:816452/1‑151 (MQ=255)
                               tACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCAcc                                                                                                                      >  2:291485/1‑151 (MQ=255)
                                aCTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCg                                                                                                                     >  3:1008138/1‑151 (MQ=255)
                                   gCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGc                                                                                                                    <  2:490811/149‑1 (MQ=255)
                                      tCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCcac                                                                                                                 >  3:173702/1‑149 (MQ=255)
                                       cACTGCCAAAAAATGGTGCCAGCAAAAACAAACCTGTGCATTTTATGGGCGAAACGGCGGCGGAAAAAATTACCGAGTGGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGAAGAACAGCTCCGGCAGCGTCATCACCGCCACAGt                                                                                                              <  3:1036038/151‑1 (MQ=255)
                                         cTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGTGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCg                                                                                                            >  1:237340/1‑151 (MQ=255)
                                           ggCTAATAATGGTGCCAGCAACAACAAAACTGTGCATTTTATGGGCGACACGGCGGCGGCAAAAATTATCGAGTTGGCGCCTGCCCCAGACAGCATAACCGCCGGTCCCCCGCAGAACAGCTCCGGCGGCGTCATCACCGCCACAGTCGt                                                                                                           <  4:288929/150‑1 (MQ=255)
                                           ggCTAAAAGGGGTGCCAGCGACAACAAAACTGTGCATTTTTTTGGCGACACGGCGGCGGCAAAAATTCTCGAGTTGGCGCCTGCCCCAGACAGCAAAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGtt                                                                                                          <  2:153476/151‑1 (MQ=255)
                                           ggCTAAAAATGGTGCCAGCGACAACAAAACTGTGATTTTTATTGGCGACACAGCGGCGGAAAAAATTATCGATTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTc                                                                                                             <  2:562388/148‑1 (MQ=255)
                                            gCTAATAATGGTGCCAGCGACAACAAAACTGTGCTTTTTATTGGCGACACAGCGGCGGAAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTCCCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTg                                                                                                         <  3:1167880/151‑1 (MQ=255)
                                                 taatGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGataat                                                                                                    >  1:896587/1‑151 (MQ=255)
                                                     ggTCCCAGCAAAAAAAAACTTGTGTTTTTTATGGGCAACACGGGGGCGGAAAAAATTATCGAGTTGGCCCCTGTCCCAGACAGCATAATCGCCGGTCCCCCGAAAAACAGCCCCGGCAGCGTCATCACCGCCACAGTCGTTGATaataat                                                                                                 <  1:597166/150‑1 (MQ=255)
                                                         ccAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCtt                                                                                            >  1:1236924/1‑151 (MQ=255)
                                                         ccAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCtt                                                                                            >  4:69462/1‑151 (MQ=255)
                                                            gcaaaaacaaAACTGTGCTTTTTATGGGCGACACAGCGGCGGAAAAAATTATCGGGTGGGCGCCTGCCCCAAACAGCATAATCGCCGGTCCCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCtt                                                                                            <  3:794326/143‑1 (MQ=255)
                                                             cGACAACAAAACTGTGCATTTTATGGGCGACACAGCGGCGAAAAAAATTATCGAGTGGGCGCCTGTCCCAGACAGCATAACCGCCGGTCCCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCttt                                                                                           <  3:1128351/148‑1 (MQ=255)
                                                               aaaacaaAACTGTGCATTTTATTGGCGCCACAGGGGCGGAAAAAATTACCGAGTTGGCGCCTGTCCCAGACAAAAAAATCGCCGGTCCCCCGAAAAACGGCCCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCgg                                                                                       <  1:466768/148‑1 (MQ=255)
                                                                caacaaAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATACTAATGGCTTTCCGGt                                                                                      >  4:189815/1‑150 (MQ=255)
                                                                 aacaaAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCgg                                                                                       >  4:466539/1‑148 (MQ=255)
                                                                 aacaaAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTg                                                                                     >  3:1395126/1‑150 (MQ=255)
                                                                 aaaaaaaCTGTGCATTTTATTGGCGACACAGCGGCGGAAAAAATTATCGATTTGGCGCCTGCCCCAGACAGAATAATCGCCGGTCCCCCGCAAAACAGCTCGGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGa                                                                                    <  2:236106/148‑1 (MQ=255)
                                                                 aaaaaaaCTGTCCATTTTATGGCCAACACAGCGCCGACAAAAATTATCGAGTGGCCGCCTGCCCCAGCCAGCATACCCGCCGGTCCCCCGCAAAACGGCTCCGGCGGCGTCACCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGt                                                                                      <  1:1209321/146‑1 (MQ=255)
                                                                  aaaaaccggtgCTTTTTATTGGCAACACGGGGGCGGAAAAATTTATCGATTTGGCGCCTGCCCAAGACAGAATAATCGCCGGTCCCCCGAAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGaa                                                                                   <  3:744431/143‑1 (MQ=255)
                                                                   caaAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGaa                                                                                   >  1:1011058/1‑150 (MQ=255)
                                                                    aaaaCTGTGCATTTTATTGGCGACACAGCGGCGGAAAAAATTATCGAGTTGGCGCCTGCCCCAGACAGCATAATCGCCGGTCCCCCGCAGAACAGCTCCGGCGGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAg                                                                                 <  2:57998/151‑1 (MQ=255)
                                                                        cTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGgtgt                                                                             >  1:41050/1‑151 (MQ=255)
                                                                         tgtgCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGtgtg                                                                            >  3:823691/1‑151 (MQ=255)
                                                                         tgtgCATTTTAGTGGCGACAGAGGGGCGAAAAAAATTCTCGAGTTGGGGCCTTTCCAACACAGCAAAATCGCCGGTCCCCCGGAAAACAGCTCCGGCGGCGGCAACCCCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGgtgt                                                                             <  3:852527/150‑1 (MQ=255)
                                                                           tgCATTTTATTGGCGACACAGCGGCGGAAAAAATTACCGAGTGGGCGCCTTCCCCAGACAGCATAATCGCCGGTACCCCGAAGAACGGCTCCGGCGGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGa                                                                           <  1:852503/150‑1 (MQ=255)
                                                                             cATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACt                                                                         >  4:300728/1‑150 (MQ=255)
                                                                               ttttGTTGGCGACACACCGGCGAAAAAAATTATGGATTTGGCCCCTGCCCCAGACAGAATAACCGCCGTTACCCCGAAGAACAGCCCCGGCGGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGtgtg                                                                            <  1:247695/145‑1 (MQ=255)
                                                                                 ttatggccaacacTGCGGCGGAAAAAATTATCAAGGTGGCCCATAACCCAGACAAATTAATCGCCTGTCCCCCATAAAACCGCTCGGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGa                                                                     <  1:1257957/141‑1 (MQ=255)
                                                                                 ttATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGa                                                                     >  1:864556/1‑150 (MQ=255)
                                                                                  tATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAAc                                                                   >  2:306634/1‑151 (MQ=255)
                                                                                   aTTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACt                                                                  >  3:697186/1‑151 (MQ=255)
                                                                                    tgggCGACACGGGGGCGGAAAAAATTATGGGGTGGGCGCCTGTCCCAGACACCATAACCGCCGGTCCCCCGCAGAACCGCTCGGGAGGCGCCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGaa                                                                    <  1:1184754/146‑1 (MQ=255)
                                                                                      ggCGACACAGCGGCGGCAAAAATTATCGATTTGGCGCCTGCCCCAGACAGCATAACCGCCGTTCCCCCGCAGAACAGCTCCGGCGGGGTCATCCCCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCa                                                               <  2:132946/151‑1 (MQ=255)
                                                                                      ggCGACACAGCGGCGGAAAAAATTATCGAGTTGGCGCTTGCCCCAGACAGCATAATCGCCGGTCCCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCa                                                               <  1:159160/151‑1 (MQ=255)
                                                                                      ggCGACAAGGGGGCGAAAAAAATAAACGATTGGGCGCATGTCCCAAAAAACATATTCGCCGGTCCCCCGCAGAACAGCCCCGGCATCATCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACtt                                                                 <  3:1380212/149‑1 (MQ=255)
                                                                                      ggCAACACCGCGGCGGAAAAAATTAACGGGTGGGCGCTTATCCCAACAAGAAAACTCGCCGATACCCCGCAAAACAGCCCGGGGAGTATAATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACtt                                                                 <  4:429134/149‑1 (MQ=255)
                                                                                          acacAGCGGCGAAAAAAATTATGGAGTGGGCCCCTGCCCCAGACAGCATAACCGCCGGTCCCCCGAAGAACAGCTCGGGCGGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCa                                                            <  1:248774/150‑1 (MQ=255)
                                                                                                 ggcggaAAAAATTATCGAGTGGGCCCCTGCCCAAGACAGAATAACCGCCGGTCCCCCGAAGAACAGCTCCGGCGGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCaa                                                        <  1:362644/147‑1 (MQ=255)
                                                                                                 ggcgaaaaaaaTTCGCGAGTTGGCGCCTGTCCCAGACAGCATAACCGCCGGTCCCCCGAAGAACGGCTCCGGCGGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCAcc                                                             <  1:1111856/136‑1 (MQ=255)
                                                                                                     gaaaaaaTTAGCGAGTTGGCCCTTGCCCCAGACACCAAAATCGCGGTTCCCCCGAAGAACAGCCCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCg                                                 <  4:480382/148‑1 (MQ=255)
                                                                                                       aaaaaTTATGAATTTGGCGCTGGCCCCAAAAAAAATATTCGCTGATCCCCCGCAAAAAAGCTCCGGCACCCACAACACAGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACa                                              <  3:952783/151‑1 (MQ=255)
                                                                                                       aaaaaTTATCGAGTTGGCGCCTGCCCCAGACAGCATAATCGCCGGTCCCCCGCAGAACAGCCCCGGCGGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACa                                              <  1:159843/151‑1 (MQ=255)
                                                                                                       aaaaaTAACTGAGTGGGCGTCTGCCCCAGCCAGCTTACCCGCAGGCCCCCCGCAAAGCAGCCCGGGCGGAGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACa                                              <  1:400652/151‑1 (MQ=255)
                                                                                                        aaaaTTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGAc                                               >  1:161868/1‑149 (MQ=255)
                                                                                                        aaaaTTATCGAGTGGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCGGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAg                                             <  2:294711/151‑1 (MQ=255)
                                                                                                          aattcccGAGTGGGCGCCTCCCCCAGACAGCAAAATCGCCGTTCCCCCGCAGAACAGCTCCGGCAGCTTCATCCCCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGcc                                           <  4:79225/145‑1 (MQ=255)
                                                                                                                 gggTTGGCGCCTGCCCCAGACAGCAAAACCGCCGGTCCCCCGCAGAACAGCCCCGGCGGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATg                                     <  3:711713/148‑1 (MQ=255)
                                                                                                                 gAGTTGGCGCCTCCCCCAGACAGCATATCCGCCGGTCCCCCGCAGAACAGCTCGGGCGGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGaa                                        <  1:468602/147‑1 (MQ=255)
                                                                                                                    ttGGCCCCTTCCCCAGACAGCATAATCCCCGGTCCCCCCAAAAACAGCCCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACg                                  <  3:445461/150‑1 (MQ=255)
                                                                                                                        ccccttccccAGACACAATAATCGCCGGTCCCCCGCAAAAAAGCTCCGGCAGCTTCATCGCCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATg                                     <  3:797852/136‑1 (MQ=255)
                                                                                                                         gcCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAAcggc                            >  1:662892/1‑151 (MQ=255)
                                                                                                                          ccTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAAcggcg                           >  1:1407105/1‑151 (MQ=255)
                                                                                                                          ccTGTCCCAGACAGAATAATCGCCGGTCCCCCGCAGAACAGCTCCGGCGGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAAcgg                             <  3:53401/149‑1 (MQ=255)
                                                                                                                          ccTGTCCCAAACAGCATAATCGCCGGTCCCCCGCAGAACAGCTCCGGCAGCTTCACCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAAcgg                             <  3:237360/149‑1 (MQ=255)
                                                                                                                           cTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAAcggcgg                          >  3:572997/1‑151 (MQ=255)
                                                                                                                              tCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTc                        >  2:499474/1‑150 (MQ=255)
                                                                                                                              cccaaGACAACATAACCGCGGGTCCCCCGCAAAACAGCTCCGGCGGCGTAATCCCCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAAcg                              <  1:1300853/140‑1 (MQ=255)
                                                                                                                               cccAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCa                       >  4:490549/1‑150 (MQ=255)
                                                                                                                               cccAGACAGAATAATCGCCGGTCCCCCGCAGAACAGCCCCGGCGGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAAcggcg                           <  1:502728/146‑1 (MQ=255)
                                                                                                                               cccAGACACCAAAATCGCCGGGCCCCCGCAGAACACCTCCGGCAGCTTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAAcggc                            <  3:57467/145‑1 (MQ=255)
                                                                                                                                   gACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCg                  >  2:199929/1‑151 (MQ=255)
                                                                                                                                      acaaTAACCGCCGTTCCCCCGAAAAACACCCCCGGCGGCGTCACCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGt                 <  1:794683/146‑1 (MQ=255)
                                                                                                                                      aacaaaaccGCCGGTACCCCGCAGAACAGCCCCGGCGGCTTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTg                <  1:237888/142‑1 (MQ=255)
                                                                                                                                       gCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGac              >  2:207801/1‑151 (MQ=255)
                                                                                                                                          taacccccgtaaCCCCACAGAACACCTCCGCCTGCTTAATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGacga            <  3:343796/139‑1 (MQ=255)
                                                                                                                                          tAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGacga            >  3:1171257/1‑150 (MQ=255)
                                                                                                                                           attccccGGTCCCCCGAAAAACGGCTCGGGGGGGGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGacgaa           <  2:249705/145‑1 (MQ=255)
                                                                                                                                           aaTCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGacgaac          >  3:970154/1‑151 (MQ=255)
                                                                                                                                              cGCCGGTCCCCCGAAGAACAGCCCCGCCGGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGAcgaacgaa       <  1:1225468/151‑1 (MQ=255)
                                                                                                                                                  ggTCCCCCGCAGAACAGCCCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAgg   <  2:251918/151‑1 (MQ=255)
                                                                                                                                                  ggTCCCCCGAAGAACCGCTCCGGCGGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGacgaacg         <  3:1126027/145‑1 (MQ=255)
                                                                                                                                                    tACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAggg  >  2:164437/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCGCAGGCGTTGCCTTTGGTGAGAAGACGGTTACTGCATCACTGGCTAATAATGGTGCCAGCGACAACAAAACTGTGCATTTTATTGGCGACACAGCGGCGGCAAAAATTATCGAGTTGGCGCCTGTCCCAGACAGCATAATCGCCGGTACCCCGCAGAACAGCTCCGGCAGCGTCATCACCGCCACAGTCGTTGATAATAATGGCTTTCCGGTGAAAGGTGTGACTGTGAACTTCACCAGCAACGCAGCGACAGCCGAAATGACGAACGGCGGTCAAGCCGTGACGAACGAACAGGG  >  NC_000913/2048572‑2048869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑